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We narrowed to 876 results for: NSI;

Showing: 561 - 580 of 876 results

  1. Four Factors that Differentiate the Stem Cell Field

    Type
    Blog Post
    ...site of that. 3. Stem cell science is hard, and expensive Dr. Yamanaka explained that it took 50 people ...technologies, stem cell techniques are neither fast nor inexpensive.  After talking to numerous poster presenters...

  2. R Bodies: Membrane-Rupturing Microscopic Tools

    Type
    Blog Post
    ...fluorescent protein fusions allowed us to image R bodies inside cells, and these constructs may be useful to future... S., Schleifer, K.-H., Verni, F. & Rosati, G. Defensive extrusive ectosymbionts of Euplotidium (Ciliophora...

  3. Components of CRISPR/Cas9

    Type
    Blog Post
    ...CRISPR/Cas systems have a variety of enzymes responsible for processing foreign DNA as well as the RNA...historical notes CRISPR arrays in bacterial genomes consist of repeated elements separated by unique sequences...

  4. Antibodies 101: ChIP

    Type
    Blog Post
    ...is an extremely useful technique that provides insight into protein:DNA interactions. ChIP works by using...exploring these interactions, and can give valuable insight into DNA expression and protein interactions, including...

  5. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

    Type
    Blog Post
    ... effects. However, these methods can be labor intensive and quite time consuming.   As Cas9/gRNA activity...several systems to do this but most are labor-intensive, require multiple fluorescent proteins, and aren...

  7. Developing a cold tolerant E. coli using specialized chaperones

    Type
    Blog Post
    ...inefficiently that no industrial operation would ever consider them worth the trouble.  Enter ELSA: E. coli Learning...for research.  We’ve also involved ourselves in extensive outreach programs to encourage and embolden the...

  9. Targeted Mutagenesis with EvolvR

    Type
    Blog Post
    ...the displaced strand.   Figure 1: EvolvR consists of an error-prone polymerase (PolI) that is fused...provide a better understanding of the key residues responsible for this protein-drug interaction.   The Dueber...

  11. CasPEDIA: A Functional Classification of Cas Enzymes

    Type
    Blog Post
    ...Cas enzymes and variants available. Without a comprehensive resource, it can be overwhelming to choose a...resources throughout the site. In the Experimental Considerations section on each enzyme page, you'll find a ...

  12. Viral Vectors 101: Viral Applications

    Type
    Blog Post
    ...integrated into the genome of the target cell or transiently expressed. It’s important to note that for all...vector can express your construct in target cells transiently or constitutively depending on the virus used...

  16. Multiplex Genome Editing with CRISPR-Cpf1

    Type
    Blog Post
    ...And there are the usual transfection drawbacks: transient expression and needing to work with a transfectable...For cloning, Zetsche et al used four oligos that consist of direct repeats and crRNA.  Similar to a jigsaw...

  17. Top Requested Lentivirus and AAV of 2016

    Type
    Blog Post
    ...monoclonal Cas9 background because you’ll have more consistent levels of Cas9 expression between experiments...pAAV-hSyn-DIO-hM4D(Gi)-mCherry Our current AAV inventory consists almost entirely of viruses expressing DREADDs ...

  19. Plasmids 101: Methylation and Restriction Enzymes

    Type
    Blog Post
    ... and gotten unusual or unexpected results? Or considered why DpnI will degrade your template DNA from ...level, the potential for methylation should be considered when digesting DNA. Why? Well, even though Dam...
Showing: 561 - 580 of 876 results