We narrowed to 783 results for: SCR;
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TypeBlog Post...involve needles or scalpels (sharps). Cuts and scrapes from sharp equipment are one of the most common...
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Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)
TypeBlog Post...ELISA is a modification of the sandwich ELISA as described here, the direct and indirect ELISA (more details... -
Plasmid-based Recombinant Monoclonal Antibodies: What They Are and Why You Should Be Excited About Them
TypeBlog Post...to share or remix the songs, the CDs could get scratched over time, and it was difficult to keep track ... -
Meet an Addgenie: Insights into Grad School, Career Paths, and More
TypeBlog Post... I can have on an organization beyond my job description.” Our resident bagel provider, source of energy... -
Science communication: 9 Strategies to Get Your Foot in the Door
TypeBlog Post...now. These 9 strategies will get you started: Subscribe to Career Blog Posts from Addgene 1) Twitter –... -
Overcoming the Challenges of Lentiviral Production
TypeBlog Post...systems, this system incorporates the Tet-off transcriptional activation system to drive high levels of expression... -
BeHeard Award 2018: Diseases of Glycosylation, Arginine Mutagenesis, & Neural Development
TypeBlog Post...proteins in the lab, clinicians might consider prescribing GdnHCl to patients suffering from rare diseases... -
Adenoviral Vector Production and Troubleshooting
TypeBlog Post...20 additional days at which time the cells are scraped, and lysed by multiple freeze-thaw cycles. The ... -
Rabies and Neuronal Tracing
TypeBlog Post...widely used in neuroscience. In this post, I’ll describe how rabies virus (RABV) can be used in the brain... -
Pathways Over Time Plasmids Engage Students in Functional Genomics Research
TypeBlog Post...corresponding S. cerevisiae met deficiencies (manuscript in preparation), indicating that Met protein ... -
A Guide to Designing a Scientific Poster: Content, Layout, and More
TypeBlog Post...poster format differs in many ways from writing a manuscript. If you have ever attended a scientific conference... -
Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells
TypeBlog Post... can also deliver Cas9 variants, such as a transcriptional activator dCas9-VPR, if these variants are ... -
Hot Plasmids - October 2020
TypeBlog Post...Addgene The Cas Hybrid for Multiplexed Editing and screening Applications (CHyMErA) Knockout Libraries express... -
6 Tips for Grant Writing
TypeBlog Post...are unlikely to get past the initial round of screening. I STRONGLY recommend that you ask a PI or mentor... -
Tips for Technical Support Calls
TypeBlog Post...plasmids and pooled libraries were originally described in a peer-reviewed scientific publication. We ... -
Cpf1 Update: Comparison to Cas9 and NgAgo
TypeBlog Post...for Cas9 (42 nt vs 100 nt.) However, the newly described non-CRISPR protein NgAgo is smaller than any of... -
Experimenting in an Open Source lab: from CRISPR to Cats
TypeBlog Post...manual skills at the regular microscope, and the scripting skills at the automated microscope to keep our... -
How-to: 5 Steps to a Great Panel Discussion
TypeBlog Post...organizations, I asked audience members to stand and describe unique nonprofit models that differed from the... -
Hot Plasmids: Summer 2025
TypeBlog Post... separately). Figure 5: Mechanism of ATLASCre. VAMP2 = synaptobrevin 2; At = ALFA-tag; BACE ... -
Twenty Years of Addgene Sharing: CRISPR
TypeBlog Post...double-stranded break, while modulators regulate transcription and chimeric efforts make a precise edit or ...