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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 923 results for: Tro

Showing: 561 - 580 of 923 results

  1. CRISPR 101: Any Base Transversion Editors

    Type
    Blog Post
    ... for saturation mutagenesis, and they provide a strong proof of concept for further development. ...excision repair pathway. The team behind AYBEv3 introduced the first deaminase-free base editor when they... called gTBE and gCBE, with Addgene. gTBE can introduce T → C and T → G edits, though with limited selectivity...

  2. Hot Plasmids: Fall 2024

    Type
    Blog Post
    ...degraders. But CRBN has been difficult to study in vitro, requiring either expressing it with additional ...codon-optimized for C. elegans and included artificial introns to improve expression. When using these constructs... an edited strain carrying Cas9 to allow the introduction of additional sgRNAs. Image reused from Neo ...

  3. Twenty Years of Sharing: Addgene's Viral Vector Service

    Type
    Blog Post
    ...a large number of requests for vectors in the “controls” category, which includes things like viral vectors...vectors in a variety of serotypes, including the retrograde AAVrg and the systemic capsid PHP.eB.   ...Patrick, M., Guerin, K., & Fan, M. (2023). Quality control for Adeno-associated viral vector production. Neuromethods...

  4. Avoiding the Dark Side of Fluorescent Protein Fusions with mOX FPs

    Type
    Blog Post
    ...protein instead localizes to the cytoplasm? Or more troubling, what if a significant fraction of the fusion ...these compartments using CRISPR systems should strongly consider using moxFPs over standard FPs for the...Structural Biology. His interests include the quality control of secretory proteins in the Endoplasmic Reticulum...

  5. Hot Plasmids: Summer 2024

    Type
    Blog Post
    ...epigenetic silencer Recombinant antibodies for marking astrocytes MagIC-Cryo-EM for sample enrichment and structure...determination By Mike Lacy Conventional cryogenic electron microscopy (cryo-EM) protocols typically require...glia-specific intermediate filament expressed in astrocytes throughout the brain, making it a popular target...

  6. Hot Biosensors 2022: Year-End Roundup

    Type
    Blog Post
    ...pGP-AAV-syn-jGCaMP8f-WPRE (AAV Retrograde) pGP-AAV-CAG-FLEX-jGCaMP8m-WPRE (AAV Retrograde) pGP-AAV-syn-FLEX-jGCaMP8m-WPRE...have Kd around 70 mM and 100 mM, respectively. Introducing mutations and using potassium-binding protein...

  8. Recombinase-based State Machines Enable Order-dependent Logic in vivo

    Type
    Blog Post
    ...transcriptional regulation, as long as they can control recombinase activity. For example, post-transcriptional...methods section of our paper (1). How might one troubleshoot GSRMs that don't work as expected? The successful...the parts used to build them. We recommend using strong, directional terminators and promoters with buffer...

  9. PiggyBac-ing Through the Genome Editing Field

    Type
    Blog Post
    ...the number of integration copies can be tightly controlled by titrating the transposase to transposon ratios... levels of your gene of interest, as needed. Controlling the expression levels of your gene may be particularly...splinkerette-PCR method for the isolation and sequencing of retroviral insertion sites.,” Nat. Protoc., vol. 4, no. ...

  10. An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

    Type
    Blog Post
    ...CRISPR/Cas9 allows us to delete sequences and introduce mutations and epitopes with unprecedented ease...in 1-2 kilobases of additional sequence being introduced. Cre-mediated excision of the selective cassette... ML, Jorgensen EM (2018) SapTrap vectors for introducing point mutations with unc-119+ selection. https...

  11. RNA Interference in Plant Biology: New Tools for an Old Favorite

    Type
    Blog Post
    ...and LIIbeta F 1-2 RNAi, which allows assembly of intron-spliced hpRNAs through Golden Gate cloning. Artificial...Identifying actively silenced plants Once you’ve introduced your RNAi into your organism, how do you identify...insertion of any target sequence, as well as essential control plasmids. Many thanks to our guest blogger Robert...

  12. Live and Let Dye: Self-Labeling Protein Tags

    Type
    Blog Post
    ...interaction is not covalent, later work showed that introducing a Leu28Cys mutation in eDHFR does lead to covalent...2). If you’re working with purified proteins in vitro, you can even use the same tag for multiple proteins... actin filament assembly-regulating proteins in vitro reveals complex events. a) Micrographs of a fluorescently-labeled...

  13. Plasmids 101: Modular Cloning Applications and Kits

    Type
    Blog Post
    ...happy cloning! This article was written by Susanna Stroik and Meghan Rego, with thanks to Rob Hurt for his...Plants Mediated by a Cas9 Gene Containing Multiple Introns.” Plant Communications, vol. 2, no. 2, Elsevier...Matthew S., et al. Degron-Based bioPROTACs for Controlling Signaling in CAR T Cells. Cold Spring Harbor ...

  14. Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands

    Type
    Blog Post
    ...substance (fluorophore) that has absorbed light or electromagnetic radiation at another wavelength (Heinrichs,...Heinrichs, 2009). When a fluorophore absorbs light, electrons are elevated from a ground state to an excited state...After a brief elevation in this excited state, the electron returns to its ground state by emitting a photon...

  17. CRISPR 101 eBook...en español

    Type
    Blog Post
    ...¡La primera edición de nuestro libro electrónico CRISPR 101 ya está disponible en español! Esta traducción... aumentar el acceso global a los materiales de nuestro repositorio. Este increíble regalo permitirá que...

  20. Addgene’s New Way to Help You Share Science

    Type
    Blog Post
    ...not just for a better day at the office but for stronger science as well. This blog is our way of continuing...Principal Investigator. How-to guides for troubleshooting your experiments and getting better results...
Showing: 561 - 580 of 923 results