We narrowed to 958 results for: ache

...confluent because cells like their space. If they reach confluence, they stop growing (contact inhibition.... Additional passages will allow your cells to achieve a consistent growth rate. For every minute spent...pipettes for liquid waste) should be sterilized before each use. Also, all contaminated liquid and solid waste...

...pBAD vectors for example. Protein expression from each of these vectors is controlled by a different promoter...resulting in different levels of expression from each vector; lower expression may be required if your...components of the transcription and translation machinery. These include ribosomes, RNA polymerase, tRNAs...

...to run larger panels: five laser spectral flow machines have 64 detectors, allowing for the use of up ...be distinguished using spectral flow since the machine uses the full spectral signature, not just a narrow...it is critical to include an unstained sample for each tissue type you are running in your panel if you...

...partially because they contain some of the necessary machinery to make these viruses. The cells are also easy...they require cloning specific AAV or lentiviral machinery into a baculovirus plasmid. Production of baculovirus...releasing the virus to the supernatant. The virus will reach peak production when roughly 60–70% of cells have...

... current yellow fluorescent proteins tend to photobleach rapidly, which hinders their use for long-term...Alejo Rodriguez-Fraticelli recently developed an approach called Simultaneous Tracking of Recombinase Activation... skeletal muscle, kidney, spleen, lung, heart, stomach, and brain. C) Recent studies have shown that AAV11...

... through the specimen, which often leads to photobleaching.  Furthermore, point-by-point scanning along...focus light. Therefore, there is much less photo-bleaching or photo-damage than in conventional laser scanning...215. Pubmed. W. Spalteholz. (1914) Uber das Durchsichtigmachen von menschlichen und tierischen Praparaten...

... Cas9-gRNA ribonucleoproteins? One alternative approach, which avoids many of these complications, is ...vendor, this can often be expensive. An alternative approach is to express and purify His-tagged Cas9 from ...inner ear (Zuris et al. 2015). A more targeted approach using RNPs has recently been described in vitro...

...labels (green circles in figure 2 below) can be attached to each antibody. But being big is a double-edged... to different epitopes of a primary antibody. B) Each arm of the antibody has an antigen binding site ...

...that you will contribute openness, clarity, approachability, and professionalism to team meetings and projects...second to reflect. For example, say your boss approaches your team with a project she needs one of you...to produce such great work. Say your manager approaches you with glowing feedback from a client you helped...

...information, you can fuse each FP fragment to the N- and C-terminal end of each PIP and empirically test...practice because FRET depends on a number of hard-to-achieve factors. For example, FRET requires that fusion...

...doesn't require homology-directed repair (HDR) machinery, and could thus be used in non-dividing cells....convert adenine to inosine, which the translational machinery treats like guanine. This RNA editor would permit...as assessed using RNA-seq. Since the REPAIRv1 machinery is too large to fit into adeno-associated viral...

... and coach you. There are many grant consultants who offer grant training, mentoring and coaching services...development, certification, degrees, foundations, coaching and even Twitter Chats dedicated to the Grant ...

...regions of interest in a non-biased manner. To achieve this goal, it is necessary to isolate specific ...speakers. They made an every effort to interact with each other, which is critically important, especially...slow, but if they don't stop, they may be able to achieve something that may change the rules of the game...

...Epitope tags are short antigenic peptide sequences, attached to a protein of interest (POI), that facilitate...Spaghetti Monster is a symbol of opposition to the teaching of intelligent design in public schools and was...circuits are organized and how neurons connect to each other). Combined with AAVs, FPs can be easily expressed...

...reactions are facilitated by the recombination of attachment sites from the phage (attP) and the bacteria ...coli strain sensitive to CcdB (e.g. DH5α, TOP10, Mach1). The ccdB gene is present in the donor vectors ...toxic to the cell, Gateway cloning efficiency can reach >99%.   STEP 3: Express your Gene of Interest...

...genetic manipulation still need to be developed for each organism, but researchers are optimistic that this... providing support "for development of tools, approaches, and infrastructure necessary for direct tests...said they were hard to raise because they beat on each other in captivity. Sticklebacks demonstrate unusual...

...specificity over a single SpCas9. The drawback of this approach is that two sgRNAs targeting opposite strands ...strands must be available in moderate proximity to each other.  If having two sgRNAs isn’t feasible for your...disrupt a catalytic residue. Good news! You can achieve your precise edit without even making a break.  ...

...high-level career advice, open doors for you, and teach you valuable lessons along the way. We think it’...physically walking through the protocol, by going to each space in order to double-check you remember where...maybe you would like to take pictures/videos of a machine set up while your mentor is demonstrating it. Requests...

... types of ELISA, advantages and disadvantages of each, and some considerations when using the method. ...non-overlapping epitopes so that they do not interfere with each other. The detection antibody may either be conjugated... Overview of the advantages and disadvantages of each ELISA method Method Advantages Disadvantages...

... at the requirements in the job description. For each requirement, ask yourself if you have that skill...greeting like “Dear Hiring Manager.” Highlight your achievements with specific examples. Instead of saying “I...Soriano-Carot in September 2016 and was updated by Rachel Leeson in May 2024.     Additional Resources on...