We narrowed to 709 results for: esc

1. CRISPR Activation: A Practical Guide

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   Blog Post

   ... Signal Amplification in Gene Expression and Fluorescence Imaging. Cell 159:635–646 . https://doi.org/...

2. The Challenges of Cell Culture

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   Blog Post

   ...free of contamination and match their original description. The oldest, largest, and most diverse of these...

3. Important Considerations in Optogenetics Behavioral Experiments

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   Blog Post

   ...of mouse transgenics. Everything that has been described is intended for the mouse; however, all the steps...

4. Plasmids 101: E. coli Strains for Protein Expression

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   Blog Post

   ...don't see protein overexpression? The strains described above should generate sufficient expression levels...

5. Data Freedom: The Expansion of Data Sharing in Research Publications

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   ...issue by requiring ever more condensed methods descriptions, legends and limiting numbers of figures such...

6. Deep Mutational Scanning with One Pot Saturation Mutagenesis

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   ... The one-pot saturation mutagenesis technique described in Wrenbeck et al is a PCR-based approach for ...

7. Plasmids 101: Biotinylation

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   Blog Post

   ...Attaching polypeptides to a substrate Flow cytometry/fluorescence-activated cell sorting (FACS) In situ hybridization...

8. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

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   ...the retroviral life cycle for a more detailed description). Since the integrated portion does not contain...

9. Hot Plasmids: Summer 2024

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   Blog Post

   ... the first demonstrated use of prime editing to rescue a genetic disease phenotype in an animal model....

10. CRISPR 101: Validating Your Genome Edit

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    Blog Post

    ...bioinformatics/btz867 Haeussler M, Schönig K, Eckert H, Eschstruth A, Mianné J, Renaud J-B, Schneider-Maunoury S...

11. Transferable Skills Guide: Conflict Resolution

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    Blog Post

    ...even better. The Thomas Kilmann Conflict Model describes 5 basic approaches to conflict resolution based...

12. Multiple Plasmids at a Low Price: Kits from Addgene

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    Blog Post

    ...Bacteria Using an Orthogonal Ribosome-Controlled Fluorescent Reporter."ACS chemical biology 11.1 (2015): 31...

13. Treating Muscular Dystrophy with CRISPR Gene Editing

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    Blog Post

    ...optimized, it’s important to ascertain how long the rescue phenotype will last, and more importantly, if it...

14. How to Negotiate a Successful Lab Start Up Budget

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    Blog Post

    ...ultra sensitive equipment (RT-PCR, Microscopy, Fluorescence Scopes, Flowcytometers, etc.). After identifying...

15. CRISPR 101: Epigenetics and Editing the Epigenome

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    ...histone modifications. Like the TALE-LSD1 system described above, dCas9-LSD1 inactivates targeted enhancers...

16. How to Design Your gRNA for CRISPR Genome Editing

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    Blog Post

    ... a specific edit, such as the insertion of a fluorescent tag or the introduction of a specific mutation...

17. Viral Vectors 101: AAV Serotypes and Tissue Tropism

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    ... capsid of another. Formally, pseudotypes are described with two numbers separated by a slash: the first...

18. A History of Genome Engineering in Popular Culture

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    Blog Post

    ...3rd year graduate student in the lab of Prof. Francesco Paesani at University of California San Diego....

19. FLEx Technology and Optogenetics: Flipping the switch on gene expression with high spatial and temporal resolution

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    Blog Post

    ...PMC3225502. Boyden, Edward S., et al. "Millisecond-timescale, genetically targeted optical control of neural...

20. Plasmids 101: Using Transposons in the Lab

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    ...genome.   References and Resources References Feschotte C, Pritham EJ (2007) DNA Transposons and the Evolution...