We narrowed to 633 results for: gats

...consequently, politics. More concretely, scientists gather information about reality informing people and ...

...Agrobacterium-compatible, Cas9 containing vectors ready for Golden Gate mediated cloning of your gRNA of interest. ...

...commonplace, researchers used restriction mapping to investigate DNA sequence content by comparing the fragments...

...experimental period, as this mark was faithfully propagated in culture and in vivo. In certain cases, epigenetic...

... of Cas cleavage, off-target mutations can be mitigated by using two gRNAs in close proximity that guide...

...where individuals are empowered to productively navigate through both their failures and their successes...

... to identify which insertions were positive or negative selected for during the experiment (Sandoval-Villegas...

...and optogenetics meet, allowing scientists to investigate the fundamental basis of both brain function ...

...mainly lost in F2 animals, removing the need to segregate away co-conversion markers. Expanding beyond Cas9...

...cells decreases with every subculture. Equipment Fumigate the laminar airflow and the lab facility periodically...

...near the N’ terminus of the protein, in order to mitigate the ability of the cell to use an alternative ...

...pursue it. It can manifest via research and data gathering, regulatory efforts, policy implementation, program...

...in a vibrating nozzle, imparting a positive or negative charge to cells that fluoresce, and then passing...

...Systems: Insights from CRISPR Loci in Integrative Conjugative Elements of Acidithiobacillia. The CRISPR Journal...

...References 1. Tye KM, Deisseroth K. Optogenetic investigation of neural circuits underlying brain disease ...

... and buffers through the matrix, usually by centrifugation or vacuum. In a bead-based approach, you mix...

...error-prone non-homologous end joining (NHEJ). To mitigate this, the team introduced the enzyme HMCES, either...

... indels. Therefore, they engineered a dominant-negative mutant of the protein MLH1, a component of the...

...functionality, it seems these kinds of features are delegated to other more suitable software such as ApE or...

...viral vectors: Sendai virus is a single stranded, negative sense RNA virus. It’s a member of the Paramyxoviridae...