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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 985 results for: nin

Showing: 561 - 580 of 985 results

  1. Mentoring for Scientists: I Have a Mentor, What Now?

    Type
    Blog Post
    ...– it is the discussion after that will lead to learning. Listen to our podcast interview with Harvard...this point, consider that I try to exercise every morning, but the temptation to skip the gym is strong. ...out for me that gets me out of bed and into my running shoes. Be a mentor to become a better mentee One...

  2. "Build Your Plasmid": The Game - Play to Teach

    Type
    Blog Post
    ...media or the phones hiding under their desks than listening to me. When I got home, I saw my daughters playing...get the engagement I wanted. I therefore started planning a game that would force my students to use concepts...basic lab techniques Plasmid Land: The plasmid cloning game ...

  3. FPbase: A new community-editable fluorescent protein database

    Type
    Blog Post
    ...posts by Joachim Goedhart on the Addgene blog – outlining various important considerations when choosing...contributions (using simple web forms) while also maintaining a relatively strict database design (schema)....inaccurate data submissions, so FPbase includes a versioning system that allows users to see when a given ...

  5. Improving Transduction Efficiency with Exosome AAVs

    Type
    Blog Post
    ...supernatant containing exo-AVVs from producer cells, and then to enrich the microvesicles containing AAVs (...Exosome-Associated AAV by Overexpression of the Tetraspanin CD9." Molecular Therapy-Methods & Clinical Development...

  6. Addgene-Seeding Labs plasmid grant round 3: microbial drug resistance and biofilms, antidiabetic plant components, visualizing brain injury - and a glowing robot fish

    Type
    Blog Post
    ...fluorescent proteins and a new two-photon laser scanning microscope will allow Tsakanova to image cortical...insights into functional network interactions. Combining synthetic biology with engineering: glowing bacteria... bacterial sensor, he will construct plasmids containing promoter regions that activate fluorescent protein...

  7. Visualizing Translation at the Single Molecule Level

    Type
    Blog Post
    ...used TRICK to examine translation of Drosophila patterning gene oskar. Early in development, the osk-TRICK...with the red reporter mRNA. Yan et al. used harringtonine, a translation inhibitor that stalls ribosomes...sequences into the coding sequence and 3’ UTR. Before beginning experiments, it’s important to verify that these...

  9. Addgene Depositors Get More Citations

    Type
    Blog Post
    ...publications from these journals into two groups: those containing plasmids that the authors deposited with Addgene... other reasons for the correlation. However, determining this exactly is difficult. For example, it is... papers in the same journals! So, is all this remaining difference caused by depositing the related plasmids...

  10. DAP arrays for multiplex gene editing

    Type
    Blog Post
    ...potential in vivo applications or multiplex CRISPR screening.   Using tRNAs Luckily, a fun feature of tRNA...given tRNA (derived from human or plant source), by tuning the length of 5’ leader sequences of a given tRNA...engineering of crops or allow for sophisticated CRISPR screenings that would perturb the unprecedented number of...

  11. 5 Great Apps for Lab Life

    Type
    Blog Post
    ...mass spectrometry app. I digress. When I started learning proper cell culture technique, I tried to find...my lab we do a lot of genetic construction and cloning experiments. Not all of our lab enzymes are made...be beneficial for new researchers or students beginning to learn about biochemistry. The least this app...

  12. Plasmids 101: How to Verify Your Plasmid Using a Restriction Digest Analysis

    Type
    Blog Post
    ...cleave the DNA molecules at specific sites and 2) running the reaction on an agarose gel to determine the...the gel will ensure you keep smaller bands from running off; however, you may need to run the gel for longer...such as how to set up your digests and pouring/running a DNA gel.  More Plasmid Eductional Resources:...

  13. Advice for Scientists Starting a Lab

    Type
    Blog Post
    ... your academic lab, download Science Careers' "Running Your Lab" booklet. What has been the most surprising...with us every day and in the pub with us in the evening, wanting to know our latest results as soon as ...Resources for Early Career Scientists Science Careers: Running Your Lab (pdf) Advice for Choosing a Research Project...

  14. Targeted Mutagenesis with EvolvR

    Type
    Blog Post
    ...forward genetics harnesses this at a large scale by screening diverse libraries of genetic variants. Several...enzyme. AID, however, only changes cytidines or guanines to the other three bases, which limits the level...bacteria. Its modular nature provides versatility in defining the mutational rate and editing window of the ...

  17. Improved Plasmid Maps Powered by SnapGene

    Type
    Blog Post
    ...are commonly used for Golden Gate cloning or for CRISPR gRNA cloning. With this more functional display...display, you can start thinking about your next cloning experiment early on. Beyond these simple but powerful...

  18. A Practical Approach to Choosing the B(right)est Fluorescent Protein

    Type
    Blog Post
    ... versus eukaryote, background fluorescence). Determining practical brightness When a dish of mammalian...cells is transiently transfected with a plasmid containing a fluorescent protein (fusion) there is huge ... under realistic experimental conditions. By determining the brightness for a set of fluorescent proteins...

  19. Top Requested Lentivirus and AAV of 2016

    Type
    Blog Post
    ...Sanjana et al., 2014). It is a dual-vector system, meaning it is typically used in conjunction with a second...(Gi)-mCherry from this virus is cre-dependent, meaning that once injected, the DREADD will only be expressed... vectors and genome-wide libraries for CRISPR screening. Nat Methods. 2014 Aug;11(8):783-4. PubMed PMID...

  20. Viral Vectors 101: Biosensors

    Type
    Blog Post
    ... pair on actinin will produce different signals based on changes to the structure of actinin in low vs...associated with specific proteins of interest such as actinin. When tension is high vs. low in the cell the donor...
Showing: 561 - 580 of 985 results