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We narrowed to 932 results for: omp

Showing: 561 - 580 of 932 results

  1. Inside Addgene's 10 Year Anniversary Gala

    Type
    Blog Post
    ...could take all of the credit, the success of the company is attributed to the entire Addgene family; the... on sliders, hot dogs and a mashed potato bar (complete with all the fixings), as well as plenty of delicious...course of scientific research. What was once a complicated process of requesting and sharing plasmids has...

  2. What's New in CRISPR - March 2020

    Type
    Blog Post
    ... Blue light renders the NLS accessible and the complex enters the nucleus where dCas9-Mxi1 can repress.... In the dark, the NLS is inaccessible and the complex remains in the cytoplasm. Read the paper in Cellular...CRISPR-based DNA demethylation Figure 2: The components of Casilio-ME1. Image from Taghbalout et al.,...

  3. 2023 and 2024: Reflections and Looking Ahead

    Type
    Blog Post
    ... come in the new year. I am proud of what we accomplished in 2023 and thrilled to be sharing our plans...addition, we released the MORF collection, a comprehensive transcription factor library, in lentiviral ... efficiencies typically adopted by for-profit companies, while making use of our nonprofit status to achieve...

  4. Future of Research Conference - Remarkable Opening Session

    Type
    Blog Post
    ...dire:  The current system is unsustainable, hypercompetition is corroding values, we are shifting away ...experience and encouragement.  Dr. Sibby Anderson-Thompkins, Director of the Office of Postdoctoral Affairs...such as the National Postdoc Association Core Competencies and the Science Careers’ myIDP). Dr. Galit Lahav...

  5. The Michael Davidson Collection: One-Stop Shop for Fluorescent Proteins

    Type
    Blog Post
    ..., yellow, cyan, blue, and orange spectra. A comprehensive list of cell markers all tagged with mEmerald...color variants. The mEmerald plasmids offer a comprehensive list of readily available targets since the ...work and the stage IV lung cancer that has now prompted him to deposit the tools he and his team created...

  6. Hot Plasmids and Viral Preps - July 2021

    Type
    Blog Post
    ...of this effort, members of the Rawlins Lab have compiled a new toolkit for performing genetics studies ...workflow combines thoughtfully designed plasmid components with Fluorescence Activated Cell Sorting (FACS...in high throughput screening for agrochemical compounds that have the potential to regulate the parasitization...

  7. CRISPR 101: Drug discovery and CRISPR

    Type
    Blog Post
    ...change in pathology. If the disease pathology is complex, a model may be most effective. Once drug candidates...and generate physiologically relevant models. In complex diseases, it can aid in generation of multiple ...specificity of that target and validate the hit compound. The standard is to test the drug on models which...

  8. Year of the Camelid: Antibody Style

    Type
    Blog Post
    ...through biomedical research. The Camelid family is composed of camels, llamas, and alpacas (and their undomesticated...Arbabi-Ghahroudi, 2017)!      Figure 1: Comparison of a heavy-chain only antibody and single-domain...blog over the years and have described how they compare to other antibodies and affinity reagents. But ...

  9. Addgene's a Nonprofit? Nonprofit Awareness Day 2017

    Type
    Blog Post
    ...information.” Addgene is here to help scientists accomplish their goals. Many nonprofits must spend a lot...level of positive revenue to build reserves so the company is sustainable. The difference is what nonprofits...-oriented values. Addgenies like the nonprofit company culture as well. We work every day to do a better...

  10. Oh, The Places You Can Go: Careers in Science Communication - Curriculum Development

    Type
    Blog Post
    ... expert (SME) at eScience Labs, an educational company that creates hands-on science lab kits and digital...with faculty members to make sure the courses encompass what they want to teach.” Besides working with...lab: write!” Caitlin did this by editing 15+ comprehensive exam proposals, a few PhD theses, and occasionally...

  11. Sleeping Beauty Awakens for Genome Engineering

    Type
    Blog Post
    ...transfer in vertebrates. The system is comprised of 2 components: 1) an SB transposase, the enzyme required...efficiencies of stable gene transfer using SB100x were comparable to stable transduction efficiencies of integrating...

  12. Addgene @ Keystone: Thoughts on Precision Genome Engineering and Synbio

    Type
    Blog Post
    ...CRISPR delivery systems, modifications to the RNA components, and challenging target genomes, such as the ...foremost, from the custom software used to design complex genetic circuitry to the downstream output. Tuesday...digital and analog versions of each, and Tim Lu even compared his "memory" gene codes to a Turing machine (also...

  13. Plasmids 101: Antibiotic Resistance Genes

    Type
    Blog Post
    ...labs today are semi-synthetic or fully synthetic compounds. Antibiotics can be categorized based on whether...gene of interest is cloned into/inactivates or completely removes (in the case of Gateway cloning) one ...make sure your plasmid and E. coli strain are compatible! Check out this list of common E. coli genotypes...

  16. CRISPR Challenges: Standardization and Homology Directed Repair

    Type
    Blog Post
    ...common lexicon for genome editing studies. These complementary goals are aimed at improving translation of ... loosely paraphrased in the tweet below. The complexity and challenges of homology directed repair (HDR... I think I may have finally grasped just how complicated DNA repair pathways are. I fully admit to being...

  17. Another Pathway into Cells: iTOP

    Type
    Blog Post
    ...of their luciferase assay, they concluded that components in the buffer used for protein purification must...percentages (78-79% after two rounds of transduction.) Compared to CCP transduction, iTOP is at least four times...Astolfo et al. were eager to see if iTOP would be compatible with CRISPR/Cas9 genome editing. Although CRISPR...

  18. Advice for Moving Into Sales After Your Science Postdoc

    Type
    Blog Post
    ... life science researchers. Many biotechnology companies are looking for PhD or Master’s level scientists...have done?” and “Would their academic experience compliment this sales and marketing role?”. Studying for...entrepreneurial bug and has set up an ELISA kit company on whose website you can find some great information...

  19. Revamp Your Lab Meetings With Creative Virtual Collaboration

    Type
    Blog Post
    ...writing and this recent article published in PLOS Computational Biology (Frassl et al., 2018): “Science is increasingly...collaboratively writing a multi-authored paper. PLoS Comput Biol 14:e1006508 . https://doi.org/10.1371/journal.pcbi...Simple Rules for a Successful Collaboration. PLoS Comput Biol 3:e44 . https://doi.org/10.1371/journal.pcbi...
Showing: 561 - 580 of 932 results