We narrowed to 733 results for: son

1. Some Like it Hot: Thermostable GeoCas9

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   ...optimal growth temperature of 55 °C. For a brief comparison of GeoCas9 vs. SpCas9, see the table below and...

2. SunTag and Fluorescent Imaging

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   ...the Vale and Weissman labs took this biological lesson and created a system to amplify fluorescent signals...

3. CUT&RUN: An Improved Method for Studying Protein-DNA Interactions

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   ...interactions for subsequent sheering of DNA by sonification. Though it helps capture weaker or more transient...

4. ReFigure: Save Scientific Figures into Dashboards and Share Your Insights

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   ...data about a disease or therapeutic Side-by-side comparison of the same experiment from different cell lines...

5. Cellosaurus: A Cell Line Information Database

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   ...post was co-authored by Susanna Stroik and Rachel Leeson. Here at Addgene, we like to help share useful ...

6. Tips for Improving Your Next Manuscript

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   ...practical understanding of how to apply it. The personal interaction of critiquing others’ manuscripts ...

7. FastCloning: A Newer, Simpler Cloning Technique

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   ...tips for applying it in your own lab. Table 1: Comparison Between Standard Restriction Cloning and Fast...

8. Walking beside Academia and giving it a high five: My transition from graduate work to a position at Addgene

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   ...months down the line!) and to visit scientists in person to figure out ways we can make Addgene better and...

9. Addgene @ Keystone: Thoughts on Precision Genome Engineering and Synbio

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   ...found myself extremely grateful to my colleague Jason for choosing Simon Singh's "The Code Book" for this...

10. Choosing Your Perfect Empty Backbone

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    ... Lianna Swanson...

11. Advice for Choosing a Research Project

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    ...has multiple benchmarks. Think about what your personal goals are and set milestones along the way so ...

12. History of CRISPR Cas - A tale of survival and evolution

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    ...467-477. Bondy-Denomy J, Pawluk A, Maxwell KL, Davidson AR.  Nature. 2013 Jan; 493, 429–432. Cong L,...

13. Viral Vectors 101: Transductions

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    ... will not stably integrate (unless you used a transposon system, CRISPR targeted strategy, etc.) and episomal...

14. Scientific Peer-review: Providing Critical and Kind Feedback and Advocating for Open Science

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    ...she will start her own research group at Boyce Tompson Institute.  References Baldwin, Melinda. "Credibility...

15. Bright Monomeric Fluorescent Proteins: mNeonGreen, mTFP1, and mWasabi

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    ...Resources on Addgene.org Learn about the Michael Davidson fluorescent protein collection Check out Addgene...

16. Tetbow: Bright Multicolor Labeling for Neuronal Tracing

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    ...introduced at ~2 copies per cell per color, following a Poisson distribution. To overcome the limited expression...

17. Hot Plasmids - June 2020 - Barcoded CRISPR Library, Sparse Cell Labeling, Calcineurin Reporter, and DNA Staining Dye Alternative

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    ...purification of tTALE-eGFP and tTALE-mCherry. In comparison to intercalating fluorescent dyes which can cause...

18. Viral Genome Sequencing - A One Stop Shop for AAV Identity and Serotype Confirmation

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    ...strands that are packaged at the same frequency, we reasoned that, following DNA extraction, the [+] and [-...

19. Antibodies 101: Epitope Availability

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    ... Rachel Leeson...

20. Hot Plasmids and Viral Preps - September 2021

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    ...stop codons.    New from the viral service By Jason Nasse We regularly add new viral aliquots from our...