...designed to have increased lentiviral titer as compared to previous lentiviral vectors. It can be used both... the Addgene Website Visit our Lentiviral Guide Pages Find Lentiviral Plasmids Find CRISPR Pooled Libraries...backbone for their many gRNAs. The high titer lentivirus production afforded by lentiCRISPR v2 is key to its...purpose As its name suggests, lentiCRISPR v2 is a lentiviral transfer vector used to deliver both Cas9 and...

...producing our ready-to-use lentivirus preps. You can find our lentivirus production protocol on our website...2015 and 2016 were CRISPR plasmids), but so are lentiviral vectors, many of which are used to deliver Cas9...Transductions Getting the Most from Your Lentiviral Transduction Additional Resources on Addgene.org Check out...Learn more about our Viral Production Process Find other Viral Vectors ... of 2017 is the lentivirus envelope plasmid pMD2.G from Didier Trono’s lab! Lentivirus envelope vector...titering your lentivirus in another blog post. If you need a quick primer on how lentiviruses work, check...promoter. Envelope proteins are a key component of lentiviruses. These proteins coat the virus and help determine...

...Addgene's most popular lentiviral plasmids Overview of Lentiviral Packaging Plasmids Lentiviral Protocols and ...frequently asked lentiviral questions. This useful resource from Addgene Depositor (and lentiviral expert) Didier...HIV-derived. Get Tips on Titering Your Lentivirus Concerning safety of lentiviral transduction Q8: You mentioned....  Addgene has an extensive collection of lentiviral plasmids created for a variety of applications including... Didier Trono's lab webpage explains how lentiviral particles are produced by co-transfecting three essential...essential ingredients: the lentiviral packaging vector, a transfer vector including the gene of interest... coding for an envelope. Three generations of lentiviral packaging systems have been developed over the...

...Additional Resources: Adenovirus Guide Virus Biosafety Lentiviral Plasmids AAV Plasmids Viral Vectors 101 eBook...expression cassette. Bunz 16402 pShuttle Shuttle For production of viruses containing transgene under a chosen...promoter Vogelstein 16403 pShuttle-CMV Shuttle For production of viruses containing transgene under CMV promoter...promoter Vogelstein 16404 pAdTrack Shuttle For production of GFP-trackable viruses containing transgene...promoter Vogelstein 16405 pAdTrack-CMV Shuttle For production of GFP-trackable viruses containing transgene... plasmids Zhang 50957 RedTrackCMV Shuttle For production of mRFP-trackable viruses containing transgene...under CMV Bamburg 50958 ShuttleNSE Shuttle For production of viruses containing transgene under Neuron-...

...transcription, and integration needed for the production of lentiviral particles. It’s capable of inducible expression...the last 15 years - the majority of these being lentiviral or CRISPR tools. How many times have these plasmids...Addgene psPAX2  - psPAX2 is a 2nd generation lentiviral packaging plasmid from the Didier Trono lab that...genes. It can be used with 2nd or 3rd generation lentiviral vectors and an envelope expressing plasmid, such...PX458). lentiCRISPR v2 - This 3rd generation lentiviral transfer vector also from the Zhang lab is used...and contains an envelope protein for producing lentiviral and MuLV retroviral particles. It can be used...with packaging plasmids such as pCMV-dR8.2 dvpr (lentiviral) or pUMVC (MuLV retroviral). Fun fact: published...

...infect. Note: While both lentiviruses and γ-retroviruses use the same gene products for packaging (Gag, Pol... well as the viral LTRs, differ. As a result, lentiviral and γ-retroviral packaging plasmids are not interchangeable...using 293T cells This method is very similar to lentiviral packaging methods. The three components described...Briefly, different envelope plasmids can direct the production of virus with various tropisms.  Packaging using...found within both the 5' and 3' LTRs of retro/lentiviral plasmids. U5; Unique 5'; region at the 5' end...Swift S, Lorens J, Achacoso P, Nolan GP. Rapid production of retroviruses for efficient gene delivery to...How do engineered γ-retroviruses differ from lentiviruses? There are a few important differences between...

... consistent, high-quality protocols for lentiviral production. Bryan Roth’s lab from the University of...about virus production protocols and quality control can be found on Addgene’s Viral Production webpage)....small but growing catalog of ready-made AAV and Lentiviral preps. This new Viral Service represents Addgene...Campus have provided valuable insight into AAV production. Finally, we are blessed to have an incredibly... team took the lead on operationalizing viral production and distribution, this project involved every...Software Engineering Team (and, added in 2018, our Product Team) continues to make improvements to our website...management systems to enable efficient, tracked production and distribution of viral samples. The Office...

...work. After all, as we like to sayat Addgene, productivity is infectious. Curious which viruses researchers...lentiCas9-Blast Most of Addgene’s lentiviral inventory is based on CRISPR tools, though we do provide a...requested lentivirus and AAV of 2016. The top viruses of 2016 were (drumroll please)... Top Lentivirus: lentiCas9... Cas9-expressing lentiviruses will no longer be carried over to the gRNA lentiviruses. This means that...lentiCas9-blast lentivirus from Addgene, you could use it in conjunction with gRNA lentiviruses easily made...Viral Service and began delivering ready-to-use lentivirus and adeno-associated virus (AAV) to scientists... 2016, we expanded our viral inventory to 25 lentiviruses and 25 AAVs. These viruses have been distributed...

...destination vector creates two products: the desires expression clone and a byproduct containing the ccdB gene...Gateway cloning to generate our desired constructs: lentiviral expression of the human KRAS gene in mammalian...sites.  Method A: recombination of an attB-PCR product or plasmid with an attP donor vector. In this case...entry clone: recombination of an attB-flanked PCR product with an attP-containing donor vector.   Method...level, and experimental purpose. For mammalian lentiviral expression, we could use a vector like pLenti...gene. Since the ccdB product is toxic to the cell, Gateway cloning efficiency can reach >99%.   STEP... tube, speeding up the cloning of the attB-PCR products directly into destination vectors. The cloning...

... Viral vectors are prone to recombination In lentiviral vectors, recombination often occurs between the...full length plasmid.   Figure 1: The full lentiviral plasmid is expected to be >12 kb. However if ...small colonies. Bacteria containing the recombined product tend to grow faster, so we recommend picking the...noticed that in Stbl3 bacterial cultures with lentiviral vectors, the flat white colonies contained the... Remarkable stability of an instability-prone lentiviral vector plasmid in Escherichia coli Stbl3. 3 Biotech... (2007) Improved recombinational stability of lentiviral expression vectors using reduced-genomeEscherichia...