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We narrowed to 84 results for: pooled crispr library plasmids dna

Showing: 21 - 30 of 84 results

  1. New CRISPR Web Resources and #12DaysofCRISPR Recap

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    Blog Post
    ...Control for Pooled Libraries Day 12: New website resources: CRISPR Plasmids and Resources and CRISPR History...Chavez) Day 11: Pooled libraries: Genome-Wide Screening Using CRISPR/Cas9 and Plasmids 101: NGS Quality...the start of the CRISPR revolution, Addgene has distributed over 100,000 CRISPR plasmids. But that’s not...popular CRISPR blogs to keep them current. Here are some of the changes we’ve made: New CRISPR plasmid pages...We’ve given our CRISPR Plasmids and Resources a fresh new design, sorting the plasmids we have available...popular plasmids, we’ve also added four new plasmid pages that cover some of the hottest CRISPR tools. ...reversible CRISPR modification. In addition to these subpages, our updated CRISPR Guide and CRISPR History...

  2. Controlling for Off-target Effects with a New Genome-wide CRISPR Screen Design

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    Blog Post
    ... out this guide to pooled plasmid libraries Find all of the CRISPR Pooled Libraries currently available...genome-wide CRISPR screen design.   Bassik lab’s CRISPR knockout libraries Overview of Library To better...infected with Cas9. Validating the Library The CRISPR knockout library was validated via a growth screen...genome-wide CRISPR knockout screen to analyze the following unanswered questions about CRISPR screen design...genome-wide CRISPR/Cas9 screen design Are non-targeting guides an appropriate control? Many CRISPR screens...conditions for CRISPR knockout screens, the Bassik lab created a human knockout library that has 10 gRNAs...selecting guides. Guides were delivered as a pooled library via a lentiviral vector to cell lines that ...

  3. Hot Plasmids and Viral Preps - May 2021

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    Blog Post
    ...find all of the CRISPR plasmids available from Addgene, head over to our CRISPR Plasmids and Resources page...Hutchinson Cancer Research Center developed a pooled plasmid library encompassing all possible amino acid mutations...   The CRISPR corner By Jennifer Tsang Here are a few highlights from recent CRISPR plasmids. To find...subset of the new plasmids and viral preps in the repository through our hot plasmids articles. These articles...recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you need...need. Here's what you'll find in this post: Pooled library to study antibody escape in SARS-CoV-2 Recombinant...most anti-SARS-CoV-2 antibodies. In the library, each plasmid encodes the mutated spike protein and a ...

  4. Hot Plasmids - June 2020 - Barcoded CRISPR Library, Sparse Cell Labeling, Calcineurin Reporter, and DNA Staining Dye Alternative

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    Blog Post
    ...highlight a subset of the new plasmids in the repository through our hot plasmids articles. These articles ...recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you need...about a recent plasmid deposit please sign up here.  Human whole genome sgRNA iBAR library Article contributed...Bachle Figure 1: Initial steps in the CRISPR-pooled screening using sgRNAiBAR. Image from Zhu et...conventional CRISPR screens require a low multiplicity of infection (MOI) during cell library construction... as a DNA staining dye Article contributed by Jennifer Tsang Figure 4: Double stranded DNA bound... fusions with tTALE to “stain” DNA. TALE proteins can bind specific DNA sequences which brings the attached...

  5. Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

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    Blog Post
    ...interference plasmids Find bacterial CRISPR plasmids Read our CRISPR guide ...CRISPRa libraries Read all CRISPR blog posts Resources on Addgene.org Browse our CRISPR interference ...subtilis genome, the Mobile-CRISPRi ICE element forms a conjugative plasmid-like DNA circle and is transferred...suited for genome-wide CRISPRi library screening experiments where maintaining library diversity is important...Mobile-CRISPRi into bacteria. Top: A Tn7 transposon containing the CRISPRi components and a plasmid with...ready to move Mobile-CRISPRi into your bacteria of interest? Find Mobile-CRISPRi plasmids at Addgene! References...about other CRISPR methods for bacterial genome engineering   Learn about human CRISPRko, CRISPRi, and CRISPRa...

  6. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

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    Blog Post
    ...using Addgene’s lentiviral CRISPR tools. Schematic overview of CRISPR/Cas9 gene editing process...whole-cell extracts from the initial stable cell pool (pool) and from individual cell expansions (monoclonal...primers flanking my edits in genomic DNA and doing PCR on genomic DNA. The surveyor assay itself was relatively...described in Improved vectors and genome-wide libraries for CRISPR screening. Sanjana NE, Shalem O, Zhang F...BRDN0000561167) (Plasmid #80034) and BRAF gRNAs (BRDN0001145345 and BRDN0001146266) (Plasmid #76479 and #76480...that Surveyor nuclease cleaves double stranded DNA (dsDNA) at basepair mismatches. To use this tool to ... denature and renature the dsDNA. Upon renaturation, you will generate DNA duplexes with one strand from...

  7. How to Design Your gRNA for CRISPR Genome Editing

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    Blog Post
    ...Your CRISPR Basics with Our CRISPR Guide Find CRISPR Plasmids for Your Research Find CRISPR Pooled Libraries...How to Conduct Genome-Wide CRISPR Pooled Library Screens Read other CRISPR Blog Posts Resources on Addgene.org...John Doench. CRISPR technology has made it easier than ever both to engineer specific DNA edits and to...incorporate new information into DNA. This also requires an exogenous DNA template. HDR, however, is a very...choice. For a genome-wide library to conduct genetic screens, however, a library composed of the latter ...Gene knockout with CRISPR technology is usually accomplished by Cas9-mediated dsDNA breaks: following ...so-called base editor Cas9, which makes DNA changes in the absence of dsDNA breaks (Rees et al., 2018). For C...

  8. How to Deposit Your Plasmids with Addgene

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    Blog Post
    ...special cases below:   Pooled Library A pooled library is a set of plasmids all built with the same ...insert. Pooled libraries are normally supplied as liquid DNA with all of the plasmids in the library in a...unique plasmids in a pooled library can range from a few hundred to millions. Pooled libraries must be...Unpublished or pre-publication plasmids": Unpublished plasmids or plasmids that will not be published are...and entering a plasmid name,plasmid type, and plasmid description/experimental purpose (right). On the...with your plasmids, and adding all the plasmids at once increases the likelihood that the plasmids are given...the help center article, "Can I deposit a pooled DNA library with Addgene?" or contact us at [email protected]...

  9. Hot Plasmids: Summer 2025

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    Blog Post
    ... LARRYv2 pooled libraries for cell barcoding Addgene’s own AAV6 and AAV11 packaging plasmids Mapping anterograde...laps, Addgene has pooled libraries for any summer project. While many barcoding libraries are available for...the new plasmids, antibodies, viral preps, and more in the repository through our Hot Plasmids articles...RNA Recovery (LARRYv2) barcoding libraries. The team used these libraries to analyze pre-existing heterogeneity... LARRY barcoding libraries By Mike Lacy Whether you’re planning on lounging poolside or swimming laps,...experiments and see the difference! Find mGold2 plasmids here! Lee, J., Lai, S., et al. (2025). Bright... TnpB, so hot right now By Alyssa Shepard While CRISPR has become a household name, the search for smaller...

  10. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    ...find all of the CRISPR plasmids available from Addgene, head over to our CRISPR Plasmids and Resources page...    The CRISPR corner By Jennifer Tsang Here are a few highlights from recent CRISPR plasmids. To find...different antibiotic resistance markers. New CRISPR pooled library from Keith Joung’s and Benjamin Kleinstiver...Tonia Rex's lab.  vfCRISPR, or very fast CRISPR, is a light-inducible CRISPR system developed by Taekjip...subset of the new plasmids and viral preps in the repository through our hot plasmids articles. These articles...recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you need...inducible degron system for C. elegans New CRISPR plasmids New items from the viral service Epigenetic...
Showing: 21 - 30 of 84 results