Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

We narrowed to 885 results for: TRI

Showing: 41 - 60 of 885 results

  1. Plasmids 101: Yeast Vectors

    Type
    Blog Post
    ...marker at non-physiological levels. Scientists have tried varied approaches to combat these issues. One method...maintained when grown on media containing the missing nutrient. Scientists can exploit these host mutations by...auxotrophy. When grown on media NOT containing the nutrient, the host cells will die unless they have incorporated...  Marcy Patrick...

  2. Form Your Own Peer Mentoring Group: A How-To Guide for Scientists

    Type
    Blog Post
    ...these elusive teachers, supporters and advisors? I tried to start a more formal mentoring program at my company...They all found the support of the group to be intrinsic to their success. Their stories made it clear ...Goal setting is easier with more perspectives contributing to the process Senior people (of which there...

  3. CRISPR/Cas9 FAQs Answered!

    Type
    Blog Post
    ...for efficient recombination? A10: The most we've tried to insert so far has been 1 kb. We used homology...template after performing gDNA purification and have tried conditions with 5% DMSO or without 5% DMSO. My genomic...template, you could then: Confirm your HR by doing Restriction Fragment Length Polymorphism (RFLP) (see Figure...

  4. RNA Extraction Without A Kit

    Type
    Blog Post
    ...most widely known of these products is TRIzol® (also called TRI Reagent®, RNAzol®, QIAzol®  and sold by...homogenate and shaken. After a quick centrifugation (not ultracentrifugation!), the layers of phenol and chloroform...After homogenization in TRIzol®, insoluble material is removed via centrifugation and the supernatant is... loaded onto cesium-chloride gradients and ultracentrifuged for up to 20 hours to separate the intact ...have, access to one or more large, expensive ultracentrifuge.  Figure 1: An outline of the different...out to develop a protocol that skipped the ultracentrifugation altogether. Chomczynski and Sacchi showed... by many different companies). TRIzol® is an all-in-one acid-guanidinium-phenol solution that combines...

  6. Science communication: 9 Strategies to Get Your Foot in the Door

    Type
    Blog Post
    ...writing three years ago on a field trip in Indonesia. Since then, I have tried many things: photography, making...This post was contributed by guest blogger Sarah Schmidt, a Marie Curie Fellow at The Sainsbury Laboratory...hosted by Nature), and Addgene are open to guest contributions. If you decide to start your own blog, check...

  7. Tips for Technical Support Calls

    Type
    Blog Post
    ...answer should come soon, but not instantly. Addgene tries to get back to customers within 24 hours, and we...time at Addgene, I’m confident that these tips and tricks will apply to any biology-related customer support...

  8. Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

    Type
    Blog Post
    ...’t tell if the bags contained flora or fauna. I tried to find out what certain ingredients might be used...very welcoming team from China’s Ribobio, the illustrious speaker list attracted a host of sponsors based...and depositors. China is in one of the top 10 countries in number of plasmid requests from Addgene. ...

  9. xCas9: Engineering a CRISPR Variant with PAM Flexibility

    Type
    Blog Post
    ...answers come sooner rather than later. Once you’ve tried it, let us know how xCas9 is working for you in ...another method to create point mutations, also has a strict editing window about 15 nt upstream of the PAM....included continuous outflow of phage, increasing stringency and promoting survival of only the broadest-targeting...

  10. Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!

    Type
    Blog Post
    ...keywords close enough... If in the past you have tried to combine multiple terms in an Addgene plasmid ...plasmids from the James Trimmer Lab published in eLife. Addgene is currently distributing 94 plasmids expressing...Availability to Industry: Restrict search to plasmids which can be distributed to industry research scientists... GCaMP containing plasmids are available for distribution. If your experiment involves delivering a calcium...

  11. Google Forums Round Up: First Impressions of NgAgo

    Type
    Blog Post
    ... brain tumor biology. When not snipping DNA, he tries random things (101 lessons on Spanish, C++, guitar...DEMONSTRATE GENOME EDITING BY THIS TOOL This post was contributed by guest blogger Pooran Dewari. Any views in ...performs Sanger sequencing on select regions of all distributed plasmids as part of quality control, but does...with the NgAgo system indicates that it is very tricky to use and fussy to optimize. One reason could ...inconvenient. Another possible reason for the trickiness of NgAgo is the inability of mature NgAgo protein...enzyme." DNA-scissors (blog). Blog website. 4. Hsu, Patrick D., Eric S. Lander, and Feng Zhang. "Development...

  12. Allen Institute for Brain Science AAV Enhancer Collection

    Type
    Collection
    ...AiE0621h SYFP2 Olfactory tubercle and Fundus of striatum Striatum 214849 pAAV-AiE0621h-minBG-iCre(R297T)-BGHpA...AiE0621h Cre Olfactory tubercle and Fundus of striatum Striatum 191717 pAAV-3xSP10ins-AiE0367h_C2-minRho-SYFP2...using various delivery methods including intracerebroventricular (ICV) delivery in newborns, and retro-orbital...Isocortex Hippocampal formation Cortical subplate Striatum Pallidum Hypothalamus Cerebellum Whole brain Addgene...AiP13038 AiE0743m_3xC2 SYFP2 Cholinergic interneurons Striatum 214848 pAAV-AiE0743m_3xC2-minBG-iCre(R297T)-BGHpA...AiP13780 AiE0743m_3xC2 Cre Cholinergic interneurons Striatum 214858 pAAV-AiE0873m_3xC2-minBG-SYFP2-P2A-3XFLAG...AiP14496 AiE0873m_3xC2 SYFP2 Cholinergic interneurons Striatum 214868 pAAV-AiE0873m_3xC2-minBG-iCre(R297T)-BGHpA...

  13. Brain Armamentarium

    Type
    Collection
    ...BGHpA (Alias: CN5140) Expression of CoChR-EGFP in striatal cholinergic neurons Jonathan Ting Viviana Gradinaru...BGHpA (Alias: CN4825) Expression of iCre(R297T) in striatal medium spiny neurons Jonathan Ting Viviana Gradinaru...protein) plus separate nuclear FLAG epitope in striatal cholinergic neurons Jonathan Ting Viviana Gradinaru...BGHpA (Alias: CN4361) Expression of jGCaMP8s in striatal indirect pathway medium spiny neurons (D2 MSNs...BGHpA (Alias: CN4360) Expression of jGCaMP8s in striatal direct pathway medium spiny neurons (D1 MSNs) ...BGHpA (Alias: CN4035) Expression of CoChR-EGFP in striatal indirect pathway medium spiny neurons (D2 MSNs...BGHpA (Alias: CN4033) Expression of CoChR-EGFP in striatal direct pathway medium spiny neurons (D1 MSNs) ...

  14. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...biosensor for cyclic-di-GMP measurement Asymmetrical distribution of the second messenger c-di-GMP upon ...redox-sensitive ratiometric sensor roGFP for mitochondria or cytosol Hypoxia triggers subcellular compartmental...depdent on Cre, Flp, or Vcre Comprehensive Dual- and Triple-Feature Intersectional Single-Vector Delivery of...imaging (GECOs), including blue, green, red, and ratiometric indicators An Expanded Palette of Genetically...10.1117/1.NPh.11.2.024207. Robert Campbell Calcium Ratiometric calcium biosensors from nested reporters of green... green- and orange-emitting proteins Ratiometric Matryoshka biosensors from a nested cassette of green...anisotropy Cameleon calcium sensors Single-color, ratiometric biosensors for detecting signaling activities...

  15. Immunology Research Plasmids and Resources

    Type
    Collection
    ...KILLER, KILLER/DR5, TRAIL-R2, TRAILR2, TRICK2, TRICK2A, TRICK2B, TRICKB, ZTNFR9 TNFRSF10C tumor necrosis factor... to a specific trigger. Symbol Name Synonyms ACVR1B activin A receptor, type IB ACTRIB, ACVRLK4, ALK4,... A receptor, type IIA ACTRII, ACVR2 ACVR2B activin A receptor, type IIB ACTRIIB, ActR-IIB, MGC116908 ACVRL1...secretagogue receptor - GIP gastric inhibitory polypeptide - GIPR gastric inhibitory polypeptide receptor...FMRFAL NPPA natriuretic peptide precursor A ANF, ANP, ATFB6, CDD-ANF, PND NPPB natriuretic peptide precursor...

  16. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...CMV ALS Trina Schroer 51221 CMV-CC2 DCTN1 CMV ALS Trina Schroer 51412 CMV-p150 DCTN1 CMV ALS Trina Schroer...H1 ALS Patrick Aebischer 10881 pCCL cPPT PGK EGFP WPRE LTR H1 shSOD1mismatch SOD1 H1 ALS Patrick Aebischer...CMV Parkinson's Patrick Aebischer 36070 pAAV asyn S87A/S129A SNCA CMV Parkinson's Patrick Aebischer 36071...EGFP OPTN GFP CMV ALS Beatrice Yue 68839 pOPTN1-209-EGFP OPTN GFP CMV ALS Beatrice Yue 68840 pOPTN1-424...EGFP OPTN GFP CMV ALS Beatrice Yue 68841 pOPTN217-577-EGFP OPTN GFP CMV ALS Beatrice Yue 68842 pOPTN217-...EGFP OPTN GFP CMV ALS Beatrice Yue 68843 pOPTN425-577-EGFP OPTN GFP CMV ALS Beatrice Yue 68846 pR96L-EGFP...pR96L-EGFP OPTN GFP CMV ALS Beatrice Yue 68847 pD474N-EGFP OPTN GFP CMV ALS Beatrice Yue 68848 pE478G-EGFP OPTN...

  17. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ...Sea urchin pET mCitrine LIC cloning vector (u-mCitrine) - Bacterial Expression mCitrine-N1 - Mammalian...Expression mAmetrine 406 526 26 6 0.8 hr Monomer (A206K) pBad-mAmetrine - Bacterial Expression mAmetrine-N1 -...- Mammalian Expression mCitrine 516 529 59 5.7 Monomer (A206K) pcDNA3 mCitrine LIC cloning vector (6C)... Expression pCS2+8CmCitrine - Zebrafish/Xenopus/C.elegans/Sea urchin pCS2+8NmCitrine - Zebrafish/Xenopus...Mammalian Expression mCitrine-C1 - Mammalian Expression mCitrine-pBAD - Bacterial Expression YPet 517 530 80 ... - Mammalian Expression mAmetrine-C1 - Mammalian Expression LSSmOrange 437 572 23 5.7 2.3 hr Monomer pLSSmOrange-C1...Mammalian Plasmids - Includes tagging with mCherry, mCitrine, mCerulean Davidson Lab Plasmids - Includes many...

  18. Chemogenetics AAV Preps

    Type
    Collection
    ...pAAV-hSyn-dF-HA-KORD-IRES-mCitrine kappa-opioid receptor (KOR) - Inhibition IRES mCitrine Cre-dependent 8 Roth...-DIO-HA-hM3D(Gq)-IRES-mCitrine hM3D(Gq) - Activation HA fusion and IRES mCitrine Cre-dependent 8 Roth ...-DIO-HA-hM4D(Gi)-IRES-mCitrine hM4D(Gi) - Inhibition HA fusion and IRES mCitrine Cre-dependent 8 Roth ...pAAV-CaMKIIa-HA-hM4D(Gi)-IRES-mCitrine hM4D(Gi) - Inhibition HA fusion and IRES mCitrine none 2, 8 Roth 50476 ...pAAV-GFAP-HA-rM3D(Gs)-IRES-mCitrine rM3D(Gs) - Activation HA fusion and IRES mCitrine none 5 Roth 50478 pAAV-GFAP-hM3D...element Tag Fusion tags mCherry HA Non-fusion tags mCitrine EGFP dTomato Activity Cre-dependent Flp-dependent...

  19. Lab to Office Culture Shock

    Type
    Blog Post
    ...     Over the last decade, my peers and I have tried to move away from calling non-academic careers and...hands-on lab opportunities. You can get your hands in nitrile gloves again! The opportunities are there once ...

  20. Bioinformatics at Addgene

    Type
    Blog Post
    ...If you’ve heard of containerization, but haven’t tried it or are confused by the concept, don’t worry–you... developed for a singular purpose (ex. adapter trimming, quality score analysis, k-mer counting, or sequence...first n items. To be sure, we went through much trial and error to learn the scripting language, but our...process. For example, our bbduk process created trimmed FASTQ files as output, which we specified as input...
Showing: 41 - 60 of 885 results