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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 313 results for: bon;

Showing: 61 - 90 of 313 results

  4. In Vivo Biotinylation of Bacterial Fusion Proteins

    Type
    Blog Post
    ...amine-reactive biotin derivatives that generate covalent bonds with positively-charged amino acids. Unfortunately...create a plasmid based on the biotin ligase BirA backbone (Eric Campeau, Addgene plasmid #26624) was unsuccessful...

  5. Antibodies 101: Chimeric Antibodies

    Type
    Blog Post
    ...parental antibody (blue, rat) are cloned into a backbone expressing the constant regions from an alternative... a specific antibody are cloned into a plasmid backbone containing all constant regions, just the heavy...

  6. Viral Vectors 101: Inverted Terminal Repeats

    Type
    Blog Post
    ...immediately outside of the ITRs in the plasmid backbone and do not properly assemble, but these sequences...PMID: 31914802. Savy, A., Dickx, Y., Nauwynck, L., Bonnin, D., Merten, O.-W., & Galibert, L. (2017). Impact...

  7. K. phaffii: Rising to the Occasion

    Type
    Blog Post
    ...Number of chromosomes 16 3 4 Preferred carbon source Glucose Glucose Glucose, methanol,...meaning it can utilize methanol as its sole source of carbon and energy (as well as glucose or glycerol). K....

  8. Plasmids 101: Mammalian Vectors

    Type
    Blog Post
    ...Check Out Addgene's Empty Backbone Resource: Choosing Your Perfect Plasmid Backbone Read More: Plasmids...

  9. Building and Validating Recombinant Antibodies: A Case Study

    Type
    Blog Post
    ...create an amplicon that  was cloned into a plasmid backbone for expression of the mAb heavy and light chain...modify the CH encoding sequence within the plasmid backbone to generate R-mAbs of different subclasses, including...

  10. Antibodies 101: ChIP

    Type
    Blog Post
    ...formaldehyde or UV light to create strong but reversible bonds. This will keep the protein:DNA complexes together...sample up and then processing with a homogenizer, but bone or especially fibrous tissues may need extra steps...

  15. Plasmids 101: Blue-white Screening

    Type
    Blog Post
    ...blue-white screening Use a good control: Transform the backbone plasmid without insert. All colonies on this plate...list of Bacterial Expression Systems Find Empty Backbones for Your Experiments    ...

  16. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

    Type
    Blog Post
    ...while expressing the gene. Briefly, the vector “backbone” in this system contains ccdB. A successful insertion...propagate and prep your ccdB-containing empty backbones but should never be used when selecting for recombinant...

  17. Viral Vectors 101: Calcium Sensors

    Type
    Blog Post
    ..., and as we all know, a structural component of bones and teeth. It is an enzyme co-factor, a crosslinking...the Cameleon-based FRET sensor D1ER, and GCaMPER. Bonus: There are also Cameleon-based FRET sensors available...

  18. A Look at Addgene's QC Process

    Type
    Blog Post
    ...sequence and protein data, as well as available backbone sequences. All of these resources allow QC scientists...feature highlighted by the deposited scientist  The backbone elements based on the information provided and...

  19. Tips for Titering Your Lentiviral Preps

    Type
    Blog Post
    ...that target the common features of viral vector backbones; once validated these universal primers can be... for functional assays depending on the vector backbone. When using qPCR based methods for viral titration...

  20. Hot Plasmids: Winter 2025

    Type
    Blog Post
    ...tagging the kinesins KIF5A and KIF5B as well as backbones for generating other SNAPf-, CLIPf-, and CLAP-...biosensor in optimal proportions from a vector backbone that allows lentiviral packaging as a single payload...

  21. Plasmids 101: Degron Tags

    Type
    Blog Post
    ...Thankfully, this is a hot topic with many resources! In Bondeson et al, they review all the tags discussed here...informed choice! References and Resources References Bondeson, D.P., Mullin-Bernstein, Z., Oliver, S. et al....

  25. Plasmids 101: Optimizing Plasmid Yields

    Type
    Blog Post
    ... based on the copy number of the empty plasmid backbone or a researcher’s personal observations. If you..., buffering agents, and/or supplying additional carbon sources such as glycerol or glucose (in moderation...

  27. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    ...making the ribosome skip the synthesis of a peptide bond at the C-terminus of a 2A element, leading to separation...insert the whole multicistronic cassette into a backbone as a single unit. Although it is recommended to...

  30. Twenty Years of Sharing: Expanding Accessibility

    Type
    Blog Post
    ...compare their options for tools such as empty backbones, lentiviral packaging systems, and luciferase ...Plasmids Tetracyline Inducible Plasmids Empty Backbones Bacterial Expression Systems AAV Plasmids ...
Showing: 61 - 90 of 313 results