We narrowed to 313 results for: bon;
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TypeBlog Post...2006, we saw an increase in popularity of empty backbones for shRNA expression, such as pLKO.1 puro and ...shRNA for use as a negative control, and empty backbones such as pBABE-puro for retroviral expression and...
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Early Career Researcher Toolbox: Free Online Molecular Biology Tools
TypeBlog Post...links can help you calculate how much insert and backbone you need for different types of cloning methods...method. You can select from five different insert:backbone ratios. In-Fusion cloning molar ratio calculators... -
CRISPR Challenges: Standardization and Homology Directed Repair
TypeBlog Post...help you tackle the challenges of DNA repair (with bonus gRNA design resources.) Subscribe to CRISPR updates...biophysics can help enhance HDR. CRISPR 101: Ribonucleoprotein (RNP) Delivery The RNP delivery method consistently... -
Antibodies 101: Chimeric Antibodies
TypeBlog Post...parental antibody (blue, rat) are cloned into a backbone expressing the constant regions from an alternative... a specific antibody are cloned into a plasmid backbone containing all constant regions, just the heavy... -
In Vivo Biotinylation of Bacterial Fusion Proteins
TypeBlog Post...amine-reactive biotin derivatives that generate covalent bonds with positively-charged amino acids. Unfortunately...create a plasmid based on the biotin ligase BirA backbone (Eric Campeau, Addgene plasmid #26624) was unsuccessful... -
K. phaffii: Rising to the Occasion
TypeBlog Post...Number of chromosomes 16 3 4 Preferred carbon source Glucose Glucose Glucose, methanol,...meaning it can utilize methanol as its sole source of carbon and energy (as well as glucose or glycerol). K.... -
Viral Vectors 101: Inverted Terminal Repeats
TypeBlog Post...immediately outside of the ITRs in the plasmid backbone and do not properly assemble, but these sequences...PMID: 31914802. Savy, A., Dickx, Y., Nauwynck, L., Bonnin, D., Merten, O.-W., & Galibert, L. (2017). Impact... -
Plasmids 101: Mammalian Vectors
TypeBlog Post...Check Out Addgene's Empty Backbone Resource: Choosing Your Perfect Plasmid Backbone Read More: Plasmids... -
Plasmids 101: Sequence and Ligation Independent Cloning (SLIC)
TypeBlog Post...short sticky ends to insert genes into plasmid backbones, base pairing between these ~4 base overhangs ...Cloning Technique Choosing Your Perfect Plasmid Backbone ... -
Viral Vectors 101: Gamma-Retroviral Packaging Systems
TypeBlog Post...the plasmid backbone is meant to be descriptive of the components found within the backbone. For example... -
Viral Vectors 101: The AAV Retrograde Serotype
TypeBlog Post...works. It’s kind of like “the head bone’s connected to the neck bone,” but with about a hundred billion... -
Building and Validating Recombinant Antibodies: A Case Study
TypeBlog Post...create an amplicon that was cloned into a plasmid backbone for expression of the mAb heavy and light chain...modify the CH encoding sequence within the plasmid backbone to generate R-mAbs of different subclasses, including... -
Antibodies 101: ChIP
TypeBlog Post...formaldehyde or UV light to create strong but reversible bonds. This will keep the protein:DNA complexes together...sample up and then processing with a homogenizer, but bone or especially fibrous tissues may need extra steps... -
Synthesized by Ginkgo Bioworks, Shared by Addgene: SARS-CoV-2 Plasmids for Many Expression Systems
TypeBlog Post... SARS-CoV-2 expression plasmids in a high copy backbone with a T7 promoter driving multiple combinations...plasmids. Each combination is present in two sets of backbones which drive expression using either TEF1 or GAL1... -
Plasmids 101: Blue-white Screening
TypeBlog Post...blue-white screening Use a good control: Transform the backbone plasmid without insert. All colonies on this plate...list of Bacterial Expression Systems Find Empty Backbones for Your Experiments ... -
Plasmids 101: CcdB - The Toxic Key to Efficient Cloning
TypeBlog Post...while expressing the gene. Briefly, the vector “backbone” in this system contains ccdB. A successful insertion...propagate and prep your ccdB-containing empty backbones but should never be used when selecting for recombinant... -
A Look at Addgene's QC Process
TypeBlog Post...sequence and protein data, as well as available backbone sequences. All of these resources allow QC scientists...feature highlighted by the deposited scientist The backbone elements based on the information provided and... -
Viral Vectors 101: Calcium Sensors
TypeBlog Post..., and as we all know, a structural component of bones and teeth. It is an enzyme co-factor, a crosslinking...the Cameleon-based FRET sensor D1ER, and GCaMPER. Bonus: There are also Cameleon-based FRET sensors available... -
Tips for Titering Your Lentiviral Preps
TypeBlog Post...that target the common features of viral vector backbones; once validated these universal primers can be... for functional assays depending on the vector backbone. When using qPCR based methods for viral titration... -
Hot Plasmids: Winter 2025
TypeBlog Post...tagging the kinesins KIF5A and KIF5B as well as backbones for generating other SNAPf-, CLIPf-, and CLAP-...biosensor in optimal proportions from a vector backbone that allows lentiviral packaging as a single payload... -
Plasmids 101: Degron Tags
TypeBlog Post...Thankfully, this is a hot topic with many resources! In Bondeson et al, they review all the tags discussed here...informed choice! References and Resources References Bondeson, D.P., Mullin-Bernstein, Z., Oliver, S. et al.... -
Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells
TypeBlog Post...that AdV-mediated transduction of gRNA:Cas9 ribonucleoprotein complexes into transformed and non-transformed...s0006297916070063 Maddalo D, Manchado E, Concepcion CP, Bonetti C, Vidigal JA, Han Y-C, Ogrodowski P, Crippa A,... -
Chromoproteins: Colorful Proteins For Molecular Biology Experiments
TypeBlog Post...the desired mutation and excision of the vector backbone. To do this, rhamnose is added which induces expression...point will be those that have excised the vector backbone. Vectors pTOX4, pTOX5, and pTOX6 express the ... -
Starter guide to induced pluripotent stem cells (iPSCs) part 1: A renaissance in regenerative medicine
TypeBlog Post...For example, hematopoietic stem cells found in the bone marrow only differentiate into cells of peripheral... 19343304. PubMed Central PMCID: PMC2752493. 4. Bongso, A. and M. Richards, History and perspective of... -
Plasmids 101: Optimizing Plasmid Yields
TypeBlog Post... based on the copy number of the empty plasmid backbone or a researcher’s personal observations. If you..., buffering agents, and/or supplying additional carbon sources such as glycerol or glucose (in moderation... -
CRISPR 101: Mammalian Expression Systems and Delivery Methods
TypeBlog Post...generating transgenic embryos. Cas9-gRNA ribonucleoprotein complexes Purified Cas9 protein and in vitro...Topic Page Learn about CRISPR Delivery Using Ribonucleoproteins (RNPs) Read Tips for a 1st Time CRISPR User... -
Plasmids 101: Multicistronic Vectors
TypeBlog Post...making the ribosome skip the synthesis of a peptide bond at the C-terminus of a 2A element, leading to separation...insert the whole multicistronic cassette into a backbone as a single unit. Although it is recommended to... -
Plasmids 101: Choosing an Antibiotic Resistance Gene
TypeBlog Post...expression strain capable of correctly folding disulfide bonded proteins in its cytoplasm. Microbial Cell Factories...-White Screening Choosing Your Perfect Plasmid Backbone ... -
Your Lentiviral Plasmid FAQs Answered
TypeBlog Post...plasmid backbone that was designed specifically for transient expression (find some useful backbones here... -
Twenty Years of Sharing: Expanding Accessibility
TypeBlog Post...compare their options for tools such as empty backbones, lentiviral packaging systems, and luciferase ...Plasmids Tetracyline Inducible Plasmids Empty Backbones Bacterial Expression Systems AAV Plasmids ...