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We narrowed to 801 results for: MAK

Showing: 81 - 120 of 801 results

  1. Plan Your Experiment

    Type
    Guide
    ...reagents are appropriate for a given experiment. Make sure to check whether reagents are available to ...efficiency compared to NHEJ knockout. Base editors can make a limited set of mutations. Repress or Interfere...envelope plasmids provide the necessary components to make lentiviral particles (for details about lentivirus...

  2. Chemogenetics Guide

    Type
    Guide
    ...exhibited high levels of constitutive activity, making them less than ideal. DREADDs Designer Receptors... previously used in human populations in Japan, making it an attractive option for translational studies... workflows (such as laser or LED illumination), making LMOs a versatile option. Coupling brighter bioluminescent...

  3. Optogenetics Guide

    Type
    Guide
    ... invasive. Different activation wavelengths also make it possible to combine multiple opsins in the same...196: 1-28. PMID 22341318 Gradinaru V, Zhang F, Ramakrishnan C, Mattis J, Prakash R, Diester I, Goshen I,...PMID 20621963 Mattis J, Tye KM, Ferenczi EA, Ramakrishnan C, O'Shea DJ, Prakash R, Gunaydin LA, Hyun M...

  4. Gamma-Retroviral Vector Guide

    Type
    Guide
    ... gamma-retroviral vectors have many aspects that make them useful research tools in a variety of cell ... long-term, constitutive expression. In order to make stable cell lines, VSV-G is often put under an inducible...Lines Gamma-retroviral vectors are often used to make stable cell lines. Like their popular lentiviral...

  5. Protocol - How to Streak a Plate

    Type
    Protocol
    ...Bacteria on an LB Agar Plate You may also like... Making LB Agar Plates Bacterial Transformation Recovering...the way you would hold a pencil, so that you can make a broad stroke. Only touch the surface of the plate... diagonal lines in another section of the plate. Make sure that the first line (and only the first) in...

  6. Gibson Assembly Protocol

    Type
    Protocol
    ... with a two-part Gibson reaction if you're only making a small change in a plasmid (such as point mutations...manufacturer's instructions. You can purchase master mix or make your own. Transform bacteria with the DNA and screen...overlapping PCR primers (>60 bp) but too short to make its own part (<150 bp). Pro-Tip Please note that...

  7. Using a Light Microscope Protocol

    Type
    Protocol
    ...of a microscope are magnification (the ability to make an image larger) and resolution (the ability to ...through the sample. However, some of that light won’t make it through the sample because different components...fine focus knob (the smaller of the focus knobs) to make minor adjustments to the focus. After you are happy...

  8. Antibody Guide

    Type
    Guide
    ...plasmids. Animals - Antibodies produced in animals make use of the animal’s immune system. The antigen of...Overview of application Collect and lyse samples to make proteins available. Tissue samples may need additional...Ready to put your new knowledge to use? Addgene now makes ready-to-use recombinant antibodies made from plasmids...

  9. AAV Purification by Iodixanol Gradient Ultracentrifugation

    Type
    Protocol
    ... of the rotor and place them in a stable rack. **Make sure not to disturb the gradient!** Collect Fractions...flow from the needle set at the 40–60% interface. Make sure that the microcentrifuge tubes are well positioned...spin, add more formulation buffer and sample and make sure to pipet back and forth a few times to mix ...

  10. Protocol - pLKO.1 – TRC Cloning Vector

    Type
    Protocol
    ... mouse genes. Addgene is working with the TRC to make this shRNA cloning vector available to the scientific... microfuge tubes (do NOT use polystyrene tubes), make a cocktail for each transfection: 1 μg pLKO.1 shRNA...scientists with high-quality goods and services. Addgene makes every effort to ensure the accuracy of its literature...typographical or other errors may occur. Addgene makes no warranty of any kind regarding the contents of...

  11. AAV ddPCR Titration

    Type
    Protocol
    ...the risk of contaminating reagents we recommend making small aliquots of master mixes, primers, and probes...and place in the Biological Safety Cabinet (BSC). Make sure that the BSC to be used for dilution and the...wells. Cover the cartridge with the DG8 gasket, making sure that it is secure. Transfer the cartridge ...

  12. Lentivirus ddPCR Titration

    Type
    Protocol
    ...place it in the Biological Safety Cabinet (BSC). Make sure that the BSC is supplied with sufficient pipette...wells. Cover the cartridge with the DG8 gasket, making sure that it is secure. Transfer the cartridge ...the risk of contaminating reagents we recommend making small aliquots of master mixes, and primers/probe...

  13. Cloning

    Type
    Guide
    ...repeated throughout a specific DNA sequence, which can make it difficult at times to identify compatible restriction...site-specific recombination or a ligation step, making it an easy, cheap and rapid cloning method. So ...

  14. Protocol - Over-Agar Antibiotic Plating

    Type
    Protocol
    ...Over-Agar Antibiotic Plating You may also like... Making LB Agar Plates Bacterial Transformation Recovering...transformed E. coli . Over-agar spreading of antibiotic makes it easy for an investigator to conveniently plate...

  15. Protocol - How to Purify DNA from an Agarose Gel

    Type
    Protocol
    ... Rule For each sample you want to load on a gel, make 10% more volume than needed because several microliters...For example, if you want to load 1.0 μg in 10μL, make 1.1μg in 11μL. Reference Page Top Index...

  16. Protocol - How to Perform Sequence Analysis

    Type
    Protocol
    ...sequencing primers, including: Is the primer unique? Make sure the primer only anneals once within the entire...incorrect sequence. Look at the peaks in the area and make sure they are justifiable peaks. For instance, ...

  17. Protocol - Bacterial Transformation

    Type
    Protocol
    ... that increase the transformation efficiency and make bacteria more susceptible to either chemical or ... plasmids at once. To save money, many labs also make their own competent cells. This is a relatively ...

  18. Lentivirus Production

    Type
    Protocol
    ...endonuclease negative E. coli strain such as NEB stable. Make a mixture of a total of 500 μL PEI-OptiPro SFM with...-Tip There can be batch to batch variation when making the PEI working stock, therefore the ratio of μg...

  19. Pipetting Protocol

    Type
    Protocol
    ...plunger. Remove the entire pipette from the container making sure not to touch the sides of the container with...important for scientists to calibrate their pipettes to make sure that they are properly tuned to dispense the...

  21. Plasmid Cloning by PCR (with Protocols)

    Type
    Protocol
    ...In its simplest form, PCR based cloning is about making a copy of a piece of DNA and at the same time adding...restriction site (GAATTC) to the 5’ end of this primer, making our Forward Primer 5'-GAATTCATGTGGCATATCTCGAAGTAC...

  22. Coomassie Purity Stain of Recombinant Antibodies

    Type
    Protocol
    ...buffer. Fill the chamber with 1X MOPS running buffer. Make sure that the chamber is sealed. Load 5 µL of the...Pro-Tip Leaving clear lanes between samples will make quantifying the gel easier. Cover the electrophoresis...

  23. AAV Production in HEK293 Cells

    Type
    Protocol
    ...bottle up/down vigorously for 30 sec (it’s okay to make bubbles). Incubate at RT for 15 min. Note that longer...add the OptiMEM + DNA + PEI solution to the CS5. Make sure that all five layers are covered with media...

  24. CRISPR Library Amplification

    Type
    Protocol
    .... See below for options if the recombinant band makes up a significant proportion of the DNA pool. Last... weight of each pellet should be ~1-2 g. Pro-Tip Make sure to weigh the empty tube beforehand! If you’...

  25. Experimenting in an Open Source lab: from CRISPR to Cats

    Type
    Blog Post
    ...the Victoria Makerspace in Canada, which I belong to. The team at the Victoria Makerspace is composed ...known mutants at $7.00 a strain. Makerspaces like ours can also make accounts with  Addgene. We now have...of the aspects of this project that makes it ideal for makerspaces is the data is freely available, and...  Funding Makerspaces One of the main challenges when starting a new project at a makerspace is finding...following post was contributed by Derek Jacoby from Makerspace Victoria, CA Over the decades science has become...manifested itself in the creation of Hackerspaces and Makerspaces back in 2007, which function as centres for peer...lectures. There are currently around 1,000 active makerspaces around the world. Hackerspaces.org maintains ...

  26. Transferable Skills Guide: Teamwork

    Type
    Blog Post
    ...experience and make sure your next team works better. In fact, don’t wait to be invited onto a team. Make one yourself...Hershey-Chase...Who was I to dispute history? And boy did I make the right decision. If I’d spent my senior year ... For example, when it came time to update how we make and distribute kits, I helped assemble a crack team...Is the goal of your project to launch a product? Make an informed decision about something? Fix a problem...indeed. Mission team A Mission Team is assembled to make progress towards open-ended goals whose metrics ...Team, a like-minded group of co-workers who want to make our organization as environmentally friendly as ...There’s also no end-game here, unless we manage to make Addgene the most environmentally friendly organization...

  27. Thoughts on the Future of Research 2015 Boston Symposium

    Type
    Blog Post
    ...they don’t make in wages, they make up for in training. Unfortunately, these low wages also make it so it... trainees pay may also make them more attuned to the bang for their buck - make them demand more services...answered about the makeup and welfare of the many researchers out there who are making great progress on...academic research community to develop a plan to make postdoctoral pay/benefits commensurate with work...how many post-docs there are in the U.S., their makeup (in terms of nationality, gender, race, etc), or... One of the Saturday Hack Day projects sought to make an interactive game that highlights just this point...will help you in your academic publishing goals and make you a better communicator overall. Don’t Write...

  28. Is this the right place for me? 8 tactics for choosing a lab

    Type
    Blog Post
    ...reasoning out the window when making decisions. We think scientists make choices based on logic and reason...’s actually something you CAN choose and it will make a huge difference for your future career and life...Which route should I take to get to work?”; we often make important life decisions without much logic - even...your own style of work and communication before making this important decision. I’ll highlight some of...matter to me?”  and hone in on the factors that will make the most difference for your success. Gather data...of written questions for the PI and lab members.  Make coffee or beer dates to talk to as many lab alumni...energy level in the lab? Does a frenetic atmosphere make you edgy or energized?   Are you an only child and...

  29. Mentoring for Scientists: I Have a Mentor, What Now?

    Type
    Blog Post
    ...online). Mentee: Make a list of topics/skills you are interested in exploring. Mentor:  Make a list of topics...your career development as your mentor. How do you make the most of this new relationship? Consider adding...take this past the chatting stage you will rarely make any change in your skill sets or activate the helpful...that need work or growth. Here are some ideas for making your meetings productive: Discuss logistics if...communicate between meetings (email, phone, etc.)? Make agendas for your meetings, take informal minutes...goals with a mentor: The secret of accountability To make a mentoring relationship really productive, the ...many mentoring meetings to hone the goals and to make them “SMART.” SMART goals are Specific, Measurable...

  30. Will You Be My Mentor? Finding and Asking for Mentoring Support

    Type
    Blog Post
    ...Read this Catalyst Report “Making Mentoring Work” to learn about what makes a good mentoring program. ...guidance. Second, I will offer some advice on how to “make the ask” once you have found someone you admire ...professional presence, approach them for advice on what makes them that way. Is there a senior person whose career...Choose 1-2 tactics to try. One of my favorites is making the pledge to have lunch or coffee with someone...able to: Help identify the skills you will need to make a change Provide perspective on core values at another...mentoring program, consider tapping into it and helping make it work by being an educated mentee. Other opportunities...and time. Consider this option but be cautious and make sure to choose one that comes highly recommended...

  31. Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection

    Type
    Blog Post
    ...BioBrick parts to make more materials: to make more fuel, to make more chemicals, to make more medicine. ... of DNA, transcription of DNA to make RNA, translation of RNA to make proteins. That's central to everything...systems, what do you want to make? So, tell us readers, what do you want to make?   References: Jerome Bonnet...BioBricks Foundation is seeking to accomplish is to make the engineering of biology much easier. So you could...much fun and so incredibly useful that we wanted to make them free to use by everybody. (For more on these...really plugging into the "stuff sector" if you will, making chemicals and materials, and we anticipate plugging...imagination. The question is: what can you use biology to make? If you think about the BIOFAB collection and really...

  32. 10 Steps to a Perfect Science Talk

    Type
    Blog Post
    ...let’s chat about giving science talks and how to make them serve you well. The happy byproduct might just...results in the order they were performed does not make for a memorable presentation anywhere but in a lab...knowing your audience is that it can help you avoid making mistakes. I once made a joke about social science... story. Using stories is one of the best ways to make you and your presentations engaging and memorable...you are more likely to remember the point.   What makes a story? A story is a narrative connecting cause...all the time, you just need to figure out how to make it suspenseful. 4. Start preparing your talk early...them. Of course, if some of your work does relate, make sure to include that material, but don’t force it...

  33. A Guide to Designing a Scientific Poster: Content, Layout, and More

    Type
    Blog Post
    ...off your work at a conference, you need to first make your poster. While a poster generally contains the...participants can identify you. You may also wish to make creative use of a footer in your poster. This is...sections, so you can include a lot more. Font size: Making your poster readable While we are talking about...This strategy helps your sections stand out, and makes the entire poster more navigable.  Choose your content...that it will display your data most prominently, making it the star of your presentation. Portrait. This...potentially more catchy, but it can be trickier to make figures fit nicely into circular shapes rather than...color you select for the background, you’ll want to make sure that the color of your font is compatible. ...

  34. Negotiating Work and Life: How to Find the Joy

    Type
    Blog Post
    ...volunteer to lead a charitable cause.  While I can’t make it “all better” for you, I can offer some tips and...that end, here are my personal 10 commandments for making it all work – most of the time. #1 Thou shalt find... so will your work. Nobody wins, and you need to make a change. #2 Thou shalt sometimes put work ahead...management tools and tactics to plan your work and make this happen. Be realistic and: Your co-workers ...done your way. Be patient with yourself; you will make progress over time. #6 Thou shalt throw money at...and deliver your groceries. Do anything you can to make your life easier and to have more time to relax ...enthusiastically foster independence Your partner can make his or her own dinner if you are going away to a...

  35. Typing CRISPR Systems

    Type
    Blog Post
    ...doi.org/10.1038/nrmicro3569 Makarova, K. S., Wolf, Y. I., Iranzo, J., Shmakov, S. A., Alkhnbashi, O. S.,...Moya-Beltrán, A., Makarova, K. S., Acuña, L. G., Wolf, Y. I., Covarrubias, P. C., Shmakov, S. A., Silva, ...type III, and type IV. Type I Type I CRISPR systems make up the majority of Class 1, and are the most common..., F. E., Moeller, L., Kocalar, S., Oshiro, R., Makarova, K. S., Macrae, R. K., Koonin, E. V., & Zhang,...doi.org/10.1126/science.adi1910 Chylinski, K., Makarova, K. S., Charpentier, E., & Koonin, E. V. (2014...doi.org/10.1134/s0006297921100114 Koonin, E. V., & Makarova, K. S. (2019). Origins and evolution of CRISPR-Cas.../doi.org/10.1098/rstb.2018.0087 Koonin, E. V., Makarova, K. S., & Zhang, F. (2017). Diversity, classification...

  36. March for Science

    Type
    Blog Post
    ...community: Diversity makes the difference  Association for Psychological Science: Diversity Makes Better Science...formally, governments are the organizations that make and enforce the rules of the lands we live on. Rules...cannot declare that gravity change direction and make things fly up when we drop them. Scientific realities...to an earlier analogy, no amount of shouting will make an apple fall up. As scientist and politician Rush...will fall up instead of down when dropped will not make it happen. In a time when a top official can be ... the job of having evidence”, scientists want to make sure having evidence is the job of everyone in government...Immigration Pollution Healthcare Science education To make scientists relatable (17, 20). This is an effort...

  37. Dos & Don'ts When Publishing a Scientific Manuscript

    Type
    Blog Post
    ... information makes your manuscript more valuable to the research community. It also makes it more likely...Materials and methods section of their manuscript to make resources easier to identify, find back, and share...enough information to replicate your experiments, making research more reproducible and your results more...reviewers and other researchers to access the data and make up their own minds on whether the data analysis ...reliability of science publications. Peer reviewing To make sure your manuscript is perceived as a reliable ...ts when publishing a scientific manuscript Dos: Make sure your paper fits the scope of the journal you...Guidelines of the journal you are submitting to and make sure your manuscript fits the house style and prerequisites...

  38. Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility

    Type
    Blog Post
    ...organizations have been working to provide tools that make it easier to share in-depth information about reagents...FPbase are working hard to enable researchers by making it easier to find information about research tools... more descriptive and interactive protocols that make it much easier to learn new experimental techniques...Electronic lab notebooks also play a role here by making it easier to retain searchable, thorough notes ...techniques, an important component of reproducibility is making sure that others can understand your analyses and...data analysis while other organizations like Dryad make it easier for researchers to share data thereby ...published data if you need to. Similarly, FigShare also makes it easier to share data through their curation of...

  39. Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More

    Type
    Blog Post
    ... to produce constructs and edit genomes so as to make plants, and agricultural crops in particular, resistant...general. We are funded by a charity and since we are making all the tools for plant research here and validating...like for people to use them. The MoClo Plant Kit makes it really easy to clone genes for expression in ... the time. Now there are many, so if you want to make a 10-gene construct, you don’t have to worry about...plants, including many things people already use. It makes the switch to Golden Gate Cloning easier because...pathogens. It’s also easy to express genes in, which makes it a good laboratory workhorse. Tips & tricks Addgene... that you need from the kit and getting started making your own constructs with Golden Gate MoClo. The...

  40. Same Addgene, New Look - Why We Redesigned Our Homepage & Mascot

    Type
    Blog Post
    ...Did you see Blugene’s make-over and our new homepage? If you've visited Addgene’s homepage recently, ...messaging, as well as simplifying the page, and making it all look clean and easy to use.  Easily search...global navigation and menus over the past year, making it much easier to get around our site.  Community...navigation, we also wanted to emphasize the values that make Addgene unique: community, sharing, and fun. The...reagents and knowledge with one another, and Addgene make this process easy and fun every day.  With these...three values with each element. We also set out to make sure the page looked clean, professional, and modern...exploration throughout the site.  Blugene gets a make-over You may also have noticed another big update...
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