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We narrowed to 189 results for: lentiviral

Showing: 81 - 120 of 189 results

  1. Hot Plasmids and Viral Preps - January 2021

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    Blog Post
    ...flexibility. Find the engineered prime editors here A lentiviral CRISPR knockout library that targets human epigenetic...the scale of your CRISPR screens by using this lentiviral CRISPR human genome-wide library deposited by...

  2. Fluorescent Protein Guide: Empty Backbones

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    ... mRFP Tsoulfas Lab Lentiviral Plasmids - Fluorescently tag your gene for lentiviral expression Verkhusha...Prone to dimerization pLV-Azurite - Mammalian Lentiviral expression pB18cmBFPAzurite - Bacterial Expression...pCAG-GFP - Mammalian Expression pLV-eGFP - Mammalian Lentiviral Expression Ac5-STABLE1-neo - Insect Expression... Mammalian Expression pLV-mCherry - Mammalian Lentiviral Expression pCS2+8CmCherry - Zebrafish/Xenopus...

  4. How to Deposit Your Plasmids with Addgene

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    Blog Post
    ...categories (e.g., for a lentiviral vector to be used in mammalian cells, check Lentiviral and Mammalian Expression...be prone to recombination in bacteria) such as lentiviral, retroviral, and AAV plasmids, we recommend the...

  5. Plan Your Experiment

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    Collection
    ...high efficiency Lentiviral transduction Cas9 and gRNA can be present in a single lentiviral transfer vector...components to make lentiviral particles (for details about lentivirus, see our Lentivirus Guide ) Stable,...

  6. Hot Plasmids - November 2023

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    Blog Post
    ...components to reprogram delivery vehicles like lentiviral vectors, CreVLPs, and eVLPs for in vivo and in...cytoplasm and can be fluorescently labeled. Using lentiviral vectors to stably or inducibly express tagged... to target multiple delivery systems, such as lentivirus and MMLV gag, to specific cell types. It can ...

  8. New Tools Enable CRISPRa for Neuroscience Applications

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    Blog Post
    ...by the Day Lab, who developed neuron-optimized lentiviral vectors for in vitro and in vivo CRISPRa (Savell...neuronal physiology. The Day Lab’s neuron-optimized lentiviral vectors can be found here. High-throughput screening...Cas9 activators can be delivered to the CNS by lentivirus, which has a larger packaging capacity than AAV...Savell et al., 2019). This dual lentivirus system pairs ubiquitous expression of sgRNA(s) and mCherry (... such as EF-1α and CAG. Figure 2: Dual lentivirus system with ubiquitous expression of sgRNA (top...

  9. Deciphering the Mysteries of Behavior with Viral Vectors

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    Blog Post
    ...depression. The researchers found that injecting lentiviral vectors expressing BDNF in the hippocampus causes...Chandrasekar, and Jean-Luc Dreyer. "Selective lentiviral-mediated suppression of microRNA124a in the hippocampus...floating. In the same paper, the authors used lentivirus to introduce an shRNA to downregulate expression...

  12. CRISPR 101: Multiplex Expression of gRNAs

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    Blog Post
    ...Multiple Lentiviral Expression Systems (MuLE) Kit: This kit can be used to create lentiviral vectors expressing...CRISPR/Cas9-based genome engineering from a single lentiviral vector.” Nucleic Acids Research 42(19) (2014)...

  16. Viral Prep Service for Plasmids and Pooled Libraries

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    ...In-Stock Preps We offer commonly used AAV and lentiviral vectors as in-stock preps. These high-titer preps... libraries. Viral preparation options include lentivirus and AAV. Viral...Order a high-quality and ready-to-ship AAV or lentivirus prep for your next experiment today! Browse AAV...

  17. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

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    Blog Post
    ... with HIV-1. For two HIV-1 strains, Cas9/gRNA lentiviral expression significantly reduced the HIV-1 copy...PubMed Central PMCID: PMC4778041. Find CRISPR and lentiviral packaging plasmids used in this publication at...people worldwide are currently infected with the lentivirus HIV-1. Today, HIV-1 can be controlled with antiviral...

  19. CRISPR Plasmids and Resources

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    ...Request ready-to-use viral preps of select CRISPR lentiviral plasmids. CRISPR Plasmid Kits : Find groups of...

  21. 28 Hot Plasmid Technologies from 2015

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    Blog Post
    ...to create a final lentiviral vector containing up to 5 different elements. Lentiviral vectors constructed... MuLE vectors for customized lentivirus production Finding a lentiviral vector backbone with just the...collection of plasmids comprising the multiple lentiviral expression (MuLE) system from the laboratory ... combinations of genetic elements in a single lentiviral vector provides a powerful tool to modify mammalian...routes in various conditions. The plasmids and lentiviral vectors encoding these proteins can also be used...the process of creating complex polycistronic lentiviruses. The MuLE toolbox contains a series of building-block...

  24. 22 Hot Plasmid Technologies from 2014

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    Blog Post
    ...., BMC Microbiology 2011 Feb 22;11:38. Lentiviral CRISPR libraries for knockout screening New systems...Pooled Libraries webpage or read our blog post,Lentiviral CRISPR Libraries Enable Genome-Scale, Knockout...CoChR have been deposited to Addgene, including lentiviral and AAV expression vectors for certain variants...purification of Cas9 protein in Rosetta E. coli. New Lentiviral CRISPR activator and repressor plasmids from ...upon the CRISPR family of plasmids by offering a lentivirus-based mechanism for sgRNA delivery and providing...

  27. Adenovirus Plasmids

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    ...Additional Resources: Adenovirus Guide Virus Biosafety Lentiviral Plasmids AAV Plasmids Viral Vectors 101 eBook...cell types with almost 100% efficiency. Unlike lentiviruses or other retroviruses , adenoviruses do not ...

  28. Retrovirus Plasmids

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    Collection
    ...Resources: γ-Retrovirus Guide Biosafety Guide Lentiviral Plasmids AAV Plasmids Viral Vectors 101 eBook...

  31. Single-cell tracking of lineage and identity with CellTag

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    Blog Post
    ...single-cell level with CellTag CellTagging is a lentiviral based tagging method that labels cells with heritable...reprogramming and differentiation Check out our lentivirus guide Sign up to receive our Viral Vectors 101...

  33. CRISPR Guide

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    ...CRISPR libraries What Are Pooled Lentiviral CRISPR Libraries? Pooled lentiviral CRISPR libraries are a heterogeneous...genome-wide screens using CRISPR involves pooled lentiviral CRISPR libraries. Figure 8: Overview of genome-wide...heterogeneous population of lentiviral transfer vectors. In most cases, each plasmid contains an individual...Figure 8A), then cloned in a pooled format into lentiviral transfer vectors (Figure 8B). Libraries come ...library is suitable for your experiment. Although lentiviral libraries are the most popular method for CRISPR...our pooled library guide and our blog post on lentiviral pooled libraries . Browse Libraries: CRISPR Pooled...CRISPR/Cas9-based genome engineering from a single lentiviral vector. Nucleic Acids Research , 42 (19), e147...

  34. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

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    Blog Post
    ...performing and analyzing a gene edit using Addgene’s lentiviral CRISPR tools. Schematic overview of CRISPR...stably expressing Cas9 via transduction with Cas9 lentiviral vector, lentiCas9-Blast (52962-LV, Figure 1)....Lines with Lentivirus Isolating a Monoclonal Cell Population by Limiting Dilution Lentivirus Production...of A549 cells transduced with Cas9 expressing lentivirus at the indicated multiplicity of infection (MOI...

  40. Viral Vectors 101: Types of viruses

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    Blog Post
    ...-studied lentivirus is HIV, which many lentiviral tools have been derived from. Lentiviruses have slightly... with immortalized cell line cultures. Lentivirus Lentivirus is a type of retrovirus and shares many ... commonly used lab viruses: gamma-retrovirus, lentivirus, adenovirus, and adeno-associated virus. Gamma-retrovirus...gamma-retrovirus, coming in around 9 kb. Unlike gamma, lentivirus can infect both dividing and non-dividing cells...nucleus as gamma-retroviruses do. Fun fact: Since lentivirus and gamma-retrovirus are so closely related, ...specific virus? We have you covered with guides on lentivirus, retrovirus, adenovirus, and AAV. If you’re wondering...
Showing: 81 - 120 of 189 results