... able to distribute materials containing FLEx technology. Read the genOway press release for more information...

...retroviral enChIP system using CRISPR technology. Can you describe this technology and some of its potential applications...development of new biological technologies. The locus-specific ChIP technologies consisting of iChIP and enChIP... to develop new technologies. Majority of them are eager to just "import" technologies developed abroad...The Fujii lab specializes in developing novel technologies to analyze molecular mechanisms of genome functions...extensively with enChIP systems. Can you describe this technology and its advantages? Fujii: In the last several...years, my lab has been working on development of technologies for biochemical analysis of genome functions... chromatin immunoprecipitation (ChIP) (iChIP) technology, which uses locus-tagging and affinity purification...

... AAV purification with iodixanol gradient ultrafugation Overview: Iodixanol gradient ultracentrifugation...brief overview of how iodixanol purifications works, shows how to create the iodixanol gradient layers, and...master iodixanol AAV purification. If you’re new to creating gradients, practice making the iodixanol layers...feel for layering the iodixanol solutions and let me double-check that the iodixanol gradient solutions ...and freeze/thaw the lystate four times in a 95% ethanol and dry ice bath to release the virus particles...then treated with benzonase immediately before iodixanol purification. This approach gives me a bit of ...uses a gradient of different concentrations of iodixanol to separate out contaminants from an impure AAV...

...transformation technologies like those pioneered by Ewen Mullins of Ireland’s Teagasc.  Plant biotechnology is full...there are a lot of unexplored areas in plant biotechnology that are ripe with opportunity. We’ve decided...One of the biggest difficulties that plant biotechnologists face is public perception of genetic modification... crops has burdened this emerging engineering technology. Monoculture and chemical spraying, mainstays...point that people cannot imagine any use for GM technology other than to sustain those techniques. We feel... current benefits and future potential of the technology to the public in an open, responsible manner....this challenge directly by developing a plant biotechnology product specifically for consumer sales. Flowers...

...that had Platforms with a capital ‘P’ focused on technology ... has been critical to the Broad’s success ...biological questions, you need a tremendous amount of technology development alongside it.” Doench starting working...Perturbation Platform and encompasses a variety of technologies to functionally investigate the human genome...genome. Doench says he enjoys working on technology development under the Platform model at the Broad Institute...Institute because they are “not just developing technology for its own sake,” but work in true collaboration...history of RNAi left him a bit skeptical of the technology at the start of the CRISPR craze, but he says...justified… it has been just an incredibly useful technology.” He goes on to explain that, “everything that...

...recombinant DNA technologies that are now commonplace in laboratories. As scientific technologies rapidly progress...computers, cell phones, and cars become more technologically advanced leaving earlier versions obsolete,...perusing classic papers or are brainstorming new technological innovations. Let’s take a look at three historical... use of Sanger sequencing. Building upon this technology, radiolabelled nucleotides were replaced with... DNA sequencing machines (9). Today, similar technologies, sometimes referred to as sequence DNA as it...properties of nucleotides (8). Compared to older technologies nanopore-based DNA sequencing machines are relatively... is projected that nanopore-based sequencing technologies could one day sequence an entire genome for ...

...Scientist at the Broad Institute, John Doench. CRISPR technology has made it easier than ever both to engineer...is true when designing gRNAs for using CRISPR technology – the “best” gRNA depends an awful lot on what...Gene knockout by NHEJ Gene knockout with CRISPR technology is usually accomplished by Cas9-mediated dsDNA... Created with BioRender.com. Two newer technologies offer an alternative to HDR for introducing ... another target C or A in the window. Another technology, prime editing (reviewed in Anzalone et al., ... although these are still early days for this technology, and the user may need to optimize numerous parameters...CRISPRa (activation) and CRISPRi (inhibition) technologies – a nuclease-dead Cas9 (dCas9) is directed near...

...please read on to learn more about this exciting technology from the folks at Transposagen. This post was...engineering approaches. Most importantly for this technology, however, is that the transposase also enables... with no known upper limit [1]. The piggyBac™ technology can be used for numerous applications, including...of interest. Fortunately, by combing nuclease technology with the piggyBac™ system, these challenges can... DNA using the piggyBac™ transposon selection technology using a site-specific nuclease to stimulate homologous...of using piggyBac™, you could use recombinase technology (Cre/loxP) to remove the donor cassette. While...summary, the piggyBac™ transposon is a powerful technology that may be used for a variety of research applications...

...acid-guanidinium-phenol solution that combines the homogenization solution and phenol addition of the ...extraction protocol with guanidinium thiocyanate-phenol-chloroform. In this method, samples are still homogenized...using cesium-chloride gradients, water-saturated phenol, sodium acetate, and chloroform are added to the...centrifugation (not ultracentrifugation!), the layers of phenol and chloroform separate, and RNA is retained in...aqueous layer is extracted and RNA can then be isopropanol precipitated. This method reduced the time it...laboratory situation or tissue collection method, so biotechnology companies have marketed several products that...of RNA Isolation by Acid Guanidinium Thiocyanate–Phenol–Chloroform Extraction. Analytical Biochemistry ...

...simple technology,” Doudna further explained. “That’s why we’re seeing adoption of the technology in different...University.  There can be no doubt that CRISPR/Cas9 technology has been a breakthrough for the genome-editing...CRISPR for complex genetic diseases As CRISPR technology becomes increasingly more cost effective and ...of those off-target mutations when using this technology. When CRISPR therapies are being given in vivo... “A glance at genome editing with CRISPR-Cas9 technology.” Current genetics. https://doi.org/10.1007/s00294... Khatibi et al. 2021.  “CRISPR Genome Editing Technology and its Application in Genetic Diseases: A Review...Review.” Current pharmaceutical biotechnology. https://doi.org/10.2174/1389201021666200621161610 4. Uddin...

...media change into methanol and peroxisomal autophagy can be induced by removing methanol from the media....protein animal feed. Due to increasing prices of methanol and advancements in other biomass production techniques...Preferred carbon source Glucose Glucose Glucose, methanol, glycerol Heterothallic mating Efficient... a methylotropic yeast, meaning it can utilize methanol as its sole source of carbon and energy (as well... its powerful promoter of a gene important for methanol usage. This gene promoter, AOX1, was co-opted ...all eukaryotic cells and peroxisomes are where methanol metabolism occurs in methylotrophic organisms....Riley, R., et al., Comparative genomics of biotechnologically important yeasts. Proc. Natl. Acad. Sci.,...

...learn more about “... recent findings in basic immunology, cancer-immune cell interactions, cancer immunotherapy... conference was that Cancer Researchers and Immunologists are coming together to tackle the challenge ... from the conference which reflect how cancer/immunology researchers are preparing and carrying out this...inflammation and immunosurveillance have a look at our Immunology Research Plasmids and Resources. Studying the...the use of optogenetic tools is expanding to immunological research. Here the Zhou group presented Opto-CRAC...to calcium. We’d love to expand our cancer and immunology resources. If you have a great new plasmid you...Plasmids Browse Immunology Plasmids Find Kits for Cancer Research  ...

... CRISPR-based cytosine base editing. Nature Biotechnology, 41(5), 663–672. https://doi.org/10.1038/s41587...activity and therapeutic application. Nature Biotechnology, 38(7), 892–900. https://doi.org/10.1038/s41587...concurrent adenine and cytosine editing. Nature Biotechnology, 38(7), 861–864. https://doi.org/10.1038/s41587...engineered Cas9-cytidine deaminase fusions. Nature Biotechnology, 35(4), 371–376. https://doi.org/10.1038/nbt...optimization and ancestral reconstruction. Nature Biotechnology, 36(9), 843–846. https://doi.org/10.1038/nbt...editors generated from TadA variants. Nature Biotechnology, 41(5), 686–697. https://doi.org/10.1038/s41587...editor with low off-target activity. Nature Biotechnology, 41(5), 673–685. https://doi.org/10.1038/s41587...

...thanks to these technologies but many questions remain. The use of other technologies such as optogenetics...could also be tracked by cell sorter giving immunologists the ability to count cells and statistically...different immune cells. These methods allowed immunologists to develop a spatiotemporal view of an immune... time. This technique was first Introduced to immunology by three papers in the journal Science in 2002...optogenetics and CRISPR/Cas9 will help immunologists create even better tools and models to further our understanding...infected with Toxoplasma gondii." The Journal of Immunology 182.10 (2009): 6379-6393. PubMed PMID: 19414791...interfollicular region of lymph nodes." Nature immunology 9.2 (2008): 155-165. PubMed PMID: 18193049.  ...

...insecticidal proteins to the development of renewable biotechnology products and biosensors for herbicides as well...a bacterial expression system for renewable biotechnology products Dr. Idah Sithole-Niang, University ... Lecturer and a Plant Molecular Biology and Biotechnology expert. In order to preserve Cassava yields ...Robertson aims to make use of the CRISPR/Cas9 technology to engineer plants that are able to destroy DNA...Associate Professor (Right), focuses on molecular biotechnology for improving expression of nutritionally and...Bao Ton (center) is interested in applying gene technology to bacteria and plants. Her research involves...water contaminants and to subsequently develop technologies to protect fresh water repositories in Mexico...

...activity mapping with STARR-seq   Although many technologies have been developed for the study of enhancer...available from Addgene in both human and fly versions. Arnold, et al. Science. 2013. PubMed PMID: 23328393 Wu... and mutant library generation in several biotechnologically and clinically relevant bacterial species...SnoopCatcher The development of recombinant DNA technology has allowed molecular biologists to fuse fragments..., such as the food and beverage industry, the bioethanol industry, and the pharmaceutical industry, depend...through Addgene. Stovicek, et al. J Ind Microbiol Biotechnol. 2015. PubMed PMID: 26376869 Jensen, et al. FEMS...modification in Caenorhabditis elegans CRISPR/Cas9 technology has revolutionized genome editing in multiple...

... Osaka University have developed Nano-lantern technology. Nano-lanterns contain a Renilla luciferase variant...but it has precluded widespread adoption of the technology. In 2012, Saito et al. described the first Nano-lantern...luciferase (RLuc8) with Venus. This luminescent technology is based on the principle of bioluminescence ...constructs use the brightest characterized luciferase, NanoLuc, and its substrate furimazine. In addition to the...optogenetic system. Biosensors based on Nano-lantern technology are also very robust, with an eNL-based calcium...ratio. Despite the many benefits of Nano-lantern technology, there are a few drawbacks associated with luminescent... not be appropriate for use with Nano-lantern technology. Multicolor imaging using Nano-lanterns requires...

...Harshana S De Silva Feelixge. Gene therapy technologies hold great promise for improving or potentially...Rapid advances in next generation sequencing technologies have allowed scientists to quickly identify ...Almost all patients have achieved persistent immunological reconstitution with a normally functioning T...been challenging. A variety of vector delivery technologies have been investigated including lentiviruses... delivery tools such as nanoparticles. These technologies can have serious adverse effects, and their ...discovered frequently and recent advances in AAV technology have enabled us to generate new and refined forms...consideration, recent advancements in various AAV technologies have vastly improved AAVs’ potential to become...

... post was contributed by Kevin Esvelt, a Wyss Technology Development Fellow at the Wyss Institute and ...widespread trust in scientists to develop new technologies with care and humility. Nothing would damage...could not be trusted to handle such a powerful technology responsibly. An accidental release would be particularly...Finally, gene drives are an inherently collective technology: because they necessarily alter the shared environment...Thank you to our guest blogger! An inventor of technologies that harness evolution, Kevin Esvelt studies...ensuring that gene drives and other collective technologies are developed safely and transparently.  For...101: a blog series on the basics of CRISPR-Cas technology CRISPR Resources from Addgene   ...

...implications of using these technologies, but also the rapidity of the technologies’ adoption by the scientific...amongst the Nobel prize winners for developing the technology. Though these case studies are “old,” they lead... learning addresses the dilemma of creating a technology that could eventually replace scientists themselves...Addgene’s role in the rapid dissemination of these technologies, proposing that “Addgene, with its easy, fast...CRISPR-Cas plasmids, has been a key driver of the technology.” As an Addgene vet of 12 years, I’m very familiar...decline in the competitiveness of U.S. science and technology.” This last section of the book, Future Science...

...in the malaria community for the better (Nat. Biotechnol., 2014). Given the timing, it shouldn’t be a ...yet. As a genome editing tool, the CRISPR/Cas9 technology has been surrounded by a whirlwind of research... sgRNAcas9. Top tools for transferring CRISPR technology to a new organism: For obvious reasons you can...developed with the purpose of applying CRISPR technologies to new organisms and will help you whether you...Report your findings to help everyone else. CRISPR technology has reached into so many different communities...falciparum using the CRISPR-Cas9 system." Nature biotechnology(2014). PubMed PMID: 24880488. 2. Hsu, Patrick...specificity of RNA-guided Cas9 nucleases." Nature biotechnology 31.9 (2013): 827-832. PubMed PMID: 23873081....

...CRISPR-Cas nucleases in human cells." Nature biotechnology 31.9 (2013): 822-826. PubMed PMID: 23792628....on-and off-target genome editing." Trends in biotechnology 33.2 (2015): 132-140. PubMed PMID: 25595557....RNA-programmed Cas9 nuclease specificity." Nature biotechnology 31.9 (2013): 839-843. PubMed PMID: 23934178....off-target cleavage by CRISPR-Cas nucleases." Nature biotechnology 33.2 (2015): 187-197. PubMed PMID: 25513782....of zinc-finger nuclease specificity." Nature biotechnology 29.9 (2011): 816-823. PubMed PMID: 21822255....integrase-defective lentiviral vectors." Nature biotechnology 33.2 (2015): 175-178. PubMed PMID: 25599175....breaks induced by engineered nucleases." Nature biotechnology 33.2 (2015): 179-186. PubMed PMID: 25503383....

...contributed by Steph Guerra, a AAAS Science and Technology Policy Fellow at the Veterans Health Administration...roles and my current work as a AAAS Science and Technology Policy Fellow (STPF) at the Veterans Health Administration...these resources compiled by the AAAS Science and Technology Policy Fellowship Program to learn more about...could understand how to make new treatments and technologies accessible to patients. And what better place...guest blogger Steph Guerra, a AAAS Science and Technology Policy Fellow at the Veterans Health Administration... Guerra, PhD, is currently a AAAS Science and Technology Policy Fellow at the Veterans Health Administration...particularly interested in driving scientific and technological innovation that is accessible and equitable ...

... of Rogue Bioethics. On November 25, the MIT Technology Review dropped a bombshell report. A scientist... is unethical to use scientifically unproven technologies to edit a human embryo, or have a woman carry...controversial and ethically fraught rapidly emerging technology. We could do this for CRISPR/Cas9 – or any other...germline editing, we will initially limit the technology to research centers engaged in long-term, mandatory...review and decide on the future of genome editing technology. We must stop punting decisions on scientific... inform her work on emerging issues in novel technologies, including self-driving vehicles, synthetic ...

...Gram Negative. Technology Networks (2019) https://www.technologynetworks.com/immunology/articles/gram-...

...treatment can lyse cells. Alcohols, like ethanol or methanol, fix and permeabilize in a single step by...sample (1). When combined with cell sorters, this technology can be used to isolate a specific subset of cells...and further analyze that subset of cells. An immunologist, for example, might be interested in the lymphocytes...cell into distinct populations (Figure 2). An immunologist who studies B cells, might then gate the B cell... Cytometry: An Overview. Current Protocols in Immunology 120: . https://doi.org/10.1002/cpim.40 Picot ...retrospective, fundamentals and recent instrumentation. Cytotechnology 64:109–130 . https://doi.org/10.1007/s10616...

...you want to read more content related to CRISPR technology, subscribe to our CRISPR blog content.   In this... dCas12f to VP64. Read the paper in Nature Biotechnology Find the Cas12f-GE plasmids Programmed genome...of target genes and cell types. Their Nature Biotechnology paper showcases an improved design of the guide...editing efficiency. Read the paper in Nature Biotechnology Find the epegRNA plasmids Enhanced prime editing... paired pegRNAs.  Read the paper in Nature Biotechnology Find the PRIME-del plasmids  Deletion and replacement...expression in the liver.  Read the paper in Nature Biotechnology Find the PEDAR plasmids   Additional resources...

...rapidly decode the complexity? The CombiGEM-CRISPR technology was developed with the goal of providing an easy-to-use...This blog post will introduce you to this new technology, and guide you through the basics of CombiGEM-CRISPR...particularly interested in developing and applying new technologies to interrogate and understand complex biological... off-target effects of CRISPR-Cas9." Nature biotechnology (2016). PubMed PMID: 26780180. PubMed Central...lentiviral CRISPR-guide RNA library." Nature biotechnology 32.3 (2014): 267-273. PubMed PMID: 24535568....combinatorial genetics in human cells." Nature biotechnology (2015). PubMed PMID: 26280411. PubMed Central...

...University in Lisbon, Portugal. She leads the Glycoimmunology group based at the Research Unit on Applied...reported that PMM2-CDG patients suffer from immunological defects. These are characterized by a predisposition...play an essential role in almost every aspect of immunology, participating in events such as cell development... J., dos Reis Ferreira, V. & Videira, P. A. Immunological aspects of congenital disorders of glycosylation...winners! Paula Videira is the team leader of the Glycoimmunology group at UCIBIO, FCT-UNL, Portugal. Her main...role of glycans. Follow her on Facebook: @glycoimmunologygroup.     Michael McMurray is an Associate Professor...

...about Mammalian shRNA Tools for RNAi! Cre-Lox technology CAG-GFP-IRES-CRE (Plasmid #48201) This retroviral... and Cre-recombinase. The Cre-lox system is a technology that can be used to induce site-specific recombination...knockout, and selection marker removal. CRISPR technology pdCas9-humanized (Plasmid #44246) Expression ... the pLKO.1 vector can be found here. Cre-Lox technology Puro.Cre empty vector (Plasmid #17408)   This...lentiviral prep from the Addgene viral service. CRISPR technology lentiCRISPR v2 (Plasmid #52961) This is a 3rd...Maetzig T, et al. Gammaretroviral Vectors: Biology, Technology and Application. Viruses. 2011. PubMed PMID: ...

... hood with a disinfectant (most people use 70% ethanol, but you can also use Incidin wipes) before and...everything that’ll be brought under the hood with 70% ethanol, including your glove-covered hands! You should...spills do occur, they should be wiped with 70% ethanol immediately. Finally, avoid sticking pipettes into...Most cell culture media contain the pH indicator phenol red (pH range 6.0 (yellow) - 8.0 (red)). Blood ...is particularly interested in Glycobiology and Immunology. You can find Sana on LinkedIn.   References ...et al. "Gene expression profiling analysis of bisphenol A-induced perturbation in biological processes...

... covers a brief review of different basic BE technologies and their adaptation for plant genome editing...State University Kutubuddin Molla is an Ag-biotechnologist in the National Rice Research Institute, Cuttack...CRISPR-Cas9 cytidine deaminase fusion." Nature biotechnology 35.5 (2017): 441. PubMed PMID: 28346401. 6. ...with a Cas9-cytidine deaminase fusion." Nature biotechnology 35.5 (2017): 438. PubMed PMID: 28244994. 7. ...fusion of nCas9 and human APOBEC3A." Nature biotechnology (2018). PubMed PMID: 30272679. 8. Hua, Kai, ...scope in rice by using Cas9 variants." Plant biotechnology journal (2018). PubMed PMID: 30051586. 9. Hua...

...group developed the original iteration of this technology in 2007. Used to label neurons in the cerebellum...cerebellum of mice, the team appropriately named their technology Brainbow. Brainbow 1.0 used the fluorophores ... promoter. Zebrabow is an extremely versatile technology as recombination can be induced in developing...cells. Tetbow optimizes Brainbow for current technologies Tetbow (Sakaguchi et al., 2018) overcame the...multiple fields of view, whereas previous Brainbow technologies were useful for discriminating neurons within... continue to develop new iterations of these technologies, expect to see systems that use newer, more ...

...hours of culture, when glucose is abundant and ethanol concentrations are low, pADH1 displays low promoter...activity of the strong yeast promoter pTEF). As ethanol accumulates, it binds to the repressor, enabling...engineered pADH1 variants that are less sensitive to ethanol, including the “middle” ADH1 promoter pADH1m, which... which maintains activity during the late ethanol consumption phase of yeast culture. Repressible Binary... regulated expression in mammalian cells. BMC Biotechnol 6, 43 (2006). https://doi.org/10.1186/1472-6750...expression system for Bacillus. Appl Microbiol Biotechnol. 2019 Jan;103(1):303-313. doi: 10.1007/s00253...

...CRISPR and epigenetics As with many TALEN-based technologies, the advent of CRISPR has made targeting much...modification is certainly not the only CRISPR-based technology designed to alter gene expression. Fusing dCas9... genes from promoters and enhancers. Nature Biotechnology 33:510–517 . https://doi.org/10.1038/nbt.3199...regulatory elements in the human genome. Nature Biotechnology 35:561–568 . https://doi.org/10.1038/nbt.3853...programmable TALE-TET1 fusion proteins. Nature Biotechnology 31:1137–1142 . https://doi.org/10.1038/nbt.2726...modifications at endogenous enhancers. Nature Biotechnology 31:1133–1136 . https://doi.org/10.1038/nbt.2701...

...wasn’t that easy. In the 1980s, gene targeting technology was invented to introduce specific changes into...into the mouse genome. With these early technologies, a researcher would first introduce a mutation into...transgenic offspring. Although powerful, this technology is cumbersome to use, not that efficient, often...first exposure to CRISPR when he used this new technology to engineer mouse models (Wang et al., 2013, ...others around the world. Yes, finally, we have a technology, CRISPR, that is simple in concept, straightforward...for CRISPR-Cas9–mediated gene inactivation. Nat Biotechnol 32:1262–1267 . https://doi.org/10.1038/nbt.3026...

...University of Science and Technology in Shijiazhuang, defended the technology in a news article in Nature..., NgAgo. As you might expect with any nascent technology, researchers are having trouble getting NgAgo...in this informative post. Interestingly, this technology may make some uses of Site Directed Mutagenesis...scientists. You can find all of our August posts in chronological order here: Site Directed Mutagenesis by PCR...

...who recently received a Ph.D. in Biology and Biotechnology from Worcester Polytechnic Institute. What is...With the meteoric rise and diversity of CRISPR technologies, one might incorrectly relegate RNAi to history...requires the presence of dsRNA. In plants, CRISPR technologies are predominantly used to disrupt function at... throughput survival screen found that either technology was equivalently precise, but each method recovered...Orr recently earned his Ph.D. in Biology and Biotechnology from Worcester Polytechnic Institute where he...Cas9 and RNAi screens for essential genes. Nat Biotechnol 34:634–636 . https://doi.org/10.1038/nbt.3567...

...reverse transcriptase Article contributed by Will Arnold  Listen to the MarathonRT podcast segment Researchers...potential to provide improvements to existing technologies based on polymerase processivity. Please find...focuses on proteomics: developing and applying new technologies to quantify proteins in tissues, cells, and ...separate plasmid. When cells are treated with biotin–phenol and H2O2, APEX2 labels electron-rich amino acid...-APEX2 fusion.  This Sontheimer lab used this technology, which they call C-BERST (dCas9–APEX2 biotinylation...

... vectors: Enhancement in vector stability." Biotechnology and bioengineering 94.2 (2006): 322-329. PubMed...applying osmotic pressure and sodium butyrate." Biotechnology and bioengineering 42.5 (1993): 601-610. PubMed... stress." Advances in animal cell biology and technology for bioprocesses (1989): 272-278. 4. Ozturk, ...growth, metabolism, and antibody production." Biotechnology and bioengineering 37.10 (1991): 989-993. PubMed...improves adenovirus production." Journal of biotechnology 157.1 (2012): 228-236. PubMed PMID: 22154564...