...tool to advance our knowledge of brain-visceral organ signaling. The problem  Previously, there were... for monitoring how both the brain and visceral organs interact during various behaviors. Either tissue...common neuroscience tools into the brains of model organisms, including the Anikeeva lab’s work on a similar...applications are to expand the tool to different brain-organ axes. Alternatively, the system could be used to...initiative plasmid collection More resources on the Addgene blog Plasmid tools for microbiome studies Channel....org/10.1038/s41587-023-01833-5 Resources on Addgene.org Optogenetics guide Chemogenetics guide BRAIN...

...application. Even within an organization, there can be variance. At Addgene, our content team, where writing...asks for strong organizational skills. You might think, “I’d be good at that! I organized my lab data so...explain why you are interested in working for that organization or group. This is particularly important for...title of the position] at [name of the company/organization]. I am a [adjective that describes you] scientist...updating this post, I took an informal poll of Addgene hiring managers. All of them stated that a good...that particular job, your commitment to the organization's mission, or your deep interest in the lab's... in May 2024.     Additional Resources on the Addgene Blog Learn to Develop Your Professional Network...

...also our mission here at Addgene! Along with other open-science organizations, we support scientists in...their CV or grant applications. A number of organizations (such as ASAPbio) advocate for preprints, and...manuscript to a journal for formal peer review. Some organizations, including some journals, also support open ...general public (say, policymakers or community organizations) to use your work but don’t think they’ll have...the manuscript prior to submission. Preprints at Addgene Preprints are all about sharing scientific knowledge...significantly sooner. Plus, authors can include the Addgene ID for their deposited plasmids in the final journal...10.1371/journal.pbio.3000116 More resources on the Addgene blog Summer SciComm Series: Modes of Communication...

...plants available through Addgene. In addition to the plasmids described above, Addgene carries several useful... ease of use and utility in a wide variety of organisms.  While much of current CRISPR research focuses... plant species, including commonly used model organisms like Arabidopsis and Medicago truncatula and several...universal functionality of the CRISPR system in most organisms, some plant-specific changes to CRISPR components... are available to academic researchers through Addgene. CRISPR components for plant genome engineering...experimental design principles developed in other model organisms (see our CRISPR guide for common CRISPR principles...sequence) is consistent between plants and other organisms, but the promoter used to drive gRNA expression...

...critical in the business world. Companies and organizations that avoid all conflict can fall into “groupthink...individuals involved, and also for the labs, organizations, or businesses as a whole. When I’m attempting... sharing gets more challenging the bigger an organization gets, and creating new avenues of communication...should be communicated is necessary in a large organization, because confirming that every person has read... a common source of conflict. For instance, at Addgene, there are two teams whose primary role is customer...attempting to resolve conflicts within a given organization, it can help tremendously to have as much context... solutions that benefit themselves and their organization. Resolving conflicts is great, but avoiding ...

... available in your lab or organization, and approvals from the organization overseeing your work. Good... on the Addgene blog Mouse Modeling Part 1 Mouse Modeling Part 2 Five Popular Model Organisms Additional...the peritoneal cavity, or the space surrounding organs in the abdomen. They are a popular choice for administering...and to avoid potential off-target pokes to nearby organs. Restraint: scruffing Location: peritoneal cavity...Additional resources on Addgene.org Genome Engineering Guide CRISPR Mammalian Expression plasmids Cre-Lox plasmids...

...your current organization but also important to build relationships outside your organization. The mentor...mentors all around you. This third article in the Addgene Blog Mentoring series will cover 2 of the 7 mentoring...what makes a good mentoring program. If your organization has a mentoring program, consider tapping into...upcoming mentoring post in which I describe how to organize a successful peer mentoring circle. Asking someone...

... the kits Addgene offers to kickstart these applications, no matter what your model organism is. MoClo...across organisms. When using the MK system, many of the parts can be used in a variety of organisms, saving...019-46171-2. Resources on Addgene.org Modular Cloning (MoClo) Guide Addgene’s eBook Collection More resources...MoClo kit recommendations for different modal organisms and applications.    Plant applications We...starter for your general plant cloning experiments. Addgene also has two additional MoClo plant kits: Plant...libraries and tools available for certain model organisms and backgrounds, MoClo can be invaluable for more... complex editing and screening experiments in organisms that don’t yet have these tools built out. While...

...10.1186/s12870-020-02388-2 Resources on Addgene.org Addgene’s Modular Cloning Guide EcoFlex MoClo Kit...modular cloning? Modular cloning, or MoClo, is an organization scheme for multi-part DNA assembly. Simply put...other non-Golden Gate cloning methods. MoClo’s organization schema MoClo can be broken down into three hierarchical...with characterized behaviors are available from Addgene and other sources; second, characterizing a part...many guides/parts delivered) in certain model organisms. In mammals, most CRISPR libraries are assembled...targeting constructs and delivering them to these organisms via another optimized platform. Gene Stacking ...MoClo Kit MoClo Plant Parts Kit Resources on the Addgene blog High Complexity Golden Gate Assembly with...

...Production Specialist at Addgene. He is the audio engineer and producer for the Addgene Podcast.     Additional...research and to keep useful discoveries coming. At Addgene, we’re huge supporters of science communication...from Science in the News, a graduate student organization that works to promote science communication....non-scientists. These programs are all run and organized by graduate student researchers. That’s right,...Additional Resources on the Addgene Blog Check Out our Interview with Science Rapper Tom McFadden Learn about...

...Fluorescent reporters are not as well suited for imaging organs located deep in the body, like the gastrointestinal...the reporter and fail to pinpoint what tissue or organ this signal is coming from.   Acoustic reporter...where the bacteria localize in relation to internal organs. The limit of detection for arg1 expressing ECN...research? arg1 and arg2 plasmids are available from Addgene! References 1 Bourdeau, R.W., Lee-Gosselin, A....PubMed PMID: 29300010. Additional Resources on the Addgene Blog Learn more about in vivo imaging tools The...Acoustic reporter genes for noninvasive imaging of microorganisms in mammalian hosts. Nature, 553, 86-90. PubMed...fluorescent protein reporter gene Resources on Addgene.org Find plasmids for in vivo imaging with fluorescent...

...educating the public and organizing events. I contacted local professional organizations and nonprofits. I volunteered...Central Indiana Science Outreach, or CINSO. We organize fun science events for adults and professional...communication, editing, marketing, and website organization. My short experience with my blog helped immensely...establishments such as museums, professional organizations, and other nonprofits to gain support for CINSO...more people to quit the lab. Resources on the Addgene Blog Read Joanne Kamen's Blog Series on Mentoring...Browse All of Our Career Posts Resources on the Addgene.org Find Plasmids for Your Research Learn About ...

...Resources on the Addgene Blog Learn about Synthetic Biology with BioBuilder Use Addgene Videos to Learn...School Students Explore Careers at Addgene Resources on Addgene.org Find Yeast Expression Vectors Check... a multi-gene pathway to study and a reference organism where deletion strains are available. Then, staff...clone similar sequences from a less-well studied organism into overexpression plasmids. Students next transform...transform deletion strains from the reference organisms with the overexpression plasmids. Finally, students...Saccharomyces. cerevisiae, makes a great reference organism for the project because it is non-pathogenic, ...these plasmids, which have been deposited with Addgene, to successfully analyze Met protein complementation...

...in flies Resources on Addgene.org Find fluorescent protein plasmids at Addgene Read our Molecular Biology...specifically manipulate a cell type of interest in an organism, all cells of that type are affected. Previous...recombination outcome. Find SPARC plasmids at Addgene To more precisely control the amount of labeling...versatile and can be applied across cell types and organisms by replacing the transcription factor binding ...the PhiC31 recombinase can work in other model organisms including mouse and fish, SPARC is likely generalizable...s41592-018-0184-y Additional resources on the Addgene blog Learn about Gateway cloning Read about genome...

...the bench, I have worked for seven different organizations in a variety of science policy roles including...Big P policy from a post in non-governmental organizations, universities, professional societies, and industry...is non-governmental work happening at large organizations who have to develop policies for their communities...on a neglected disease area. Depending on the organization, these changes to policy could shift how society...your time in graduate school through outreach organizations and science blogs. Other avenues for skill development...development include leadership of extracurricular organizations, working in student government, and teaching...Twitter at @Steph_Guerra Additional resource on the Addgene blog: Browse our blog posts on science career ...

...for all ages. Organize your data and prepare publications or your dissertation Organize data that you...began working remotely recently? Most of us at Addgene began working from home last week to flatten the...new. We usually have yoga class twice a week at Addgene HQ, but now we’re sharing links to online yoga ...before bed will help you prepare for sleep. At Addgene, our mindfulness group is sticking with their Headspace...virtually connected with the people in your life. At Addgene, we’ve started virtual activities like team lunches...societies hosting virtual networking events. Download Addgene’s Science Career guide to learn more about career...begin working on these drafts and for a few model organisms, Micropublication will accept negative data or...

...  Check Out How Addgene Thinks About Developing Lab Management Software    Organizing and sharing your...share your lab protocols online Addgene Website: http://www.addgene.org/ If you’re working with plasmids...Recently I learned that Addgene’s pLKO.1 cloning protocol is viewed around 3,000 times each month. I ...protocols. Since we began sharing this protocol on the Addgene website in December 2006, the pLKO.1-TRC cloning... mobile) tools are available for scientists to organize and share their scientific protocols. Here are...plasmids and you decide to share them using Addgene’s repository, you can also share your associated protocols...gRNA_Cloning Vector (deposited by George Church’s lab). Addgene has also cataloged a variety of useful molecular...

... at Addgene.org Order Plasmids from Addgene! Find Free and Open Educational Resources at Addgene! ...official by registering BosLab inc. as a nonprofit organization in the state of Massachusetts. Because we’re...local companies and BosLab has grown into an organization consisting of over twenty members and volunteers...microbes. We’ve ordered a few yeast plasmids from Addgene and are eager to do our first yeast transformations...Angela Kaczmarczyk! Angela Kaczmarczyk is a co-organizer at BosLab, a community biotech laboratory in Somerville... @ZebraElement     Additional Resources on the Addgene Blog Experimenting in an Open Source Lab: from...

...an essential coenzyme that is required by all organisms for life. Biotin is only synthesized by plants...enzymes catalyze the transfer of carboxyl groups to organic acids to produce important cellular metabolites...post-translational protein modification. Most organisms have less than five enzymes in the entire proteome...for biotinylating only one lysine in the entire organism on the biotin carboxyl carrier protein (BCCP)....is unaffected by changes in pH, temperature or organic solvents. Uses of biotin in molecular biology Biotin... plasmids that contain both AviTag and BirA at Addgene! Oligonucleotide labeling Biotin can be added to...PubMed PMID: 8245422. Additional resources on the Addgene blog In vivo biotinylation of bacterial fusion...

... time to read them. Organizations: Accounts for nonprofits, research organizations, and universities often...hashtags to library entries, which can help you organize and search for papers later. Pro tip Learn more...search terms will show up in your feed. Feeds are organized by papers published each day, week, or month. ...scientific literature. Additional resources on the Addgene blog Learn why scientists should be on Twitter...

... use.  View the Addgene Plasmid Request Data Dashboard! Figure 1: The Addgene Requests Dashboard...I was excited to see that Addgene has recently added a new feature for depositing labs to its website... data on the date, material, requesting PI & organization, and country. This information can be of personal...default. Depositors can download their CSV file from Addgene by following these instructions, and then uploading... 2: Insert showing the upload function for the Addgene Requests Dashboard After the upload, the name...displays the data in a standardized way. Since Addgene encourages the public sharing of aggregated data...data only, information on requesting PI(s) & organization(s) is not shown on the dashboard. Key features...

...your organization Public Speaking Time management Managing a team Conflict resolution Organization Leadership... as Customer Support and Operations Manager at Addgene. In this role, I balance my daily tasks, meet cross-team...than you thought! When change happens at your organization, when your boss needs someone to step up, when...students, managing inventories in the lab, or organizing a research project, one can assume that you’ve...former Customer Support and Operations Manager at Addgene and is interested in nonprofit management and international...development.       Additional Resources on the Addgene Blog Get tips on writing a good cover letter Resume...likely collected some great time management and organizational skills. If you are responsible for working ...

...Awareness Organization (ADAO) are working toward such a ban, and the president of the organization, Linda...Additional Resources on the Addgene Blog Find out More About Cancer and Addgene's Cancer Collection Pages... has spread from where it originated to another organ in the body. Such misdiagnosis and late discovery...tumors from the peritoneum and any other abdominal organs to which it may have spread. Then, the surgeon ... Screening in Ghana Additional Resources on Addgene.org Check Out Our Cancer Ressearch Collection Find...

...Nonetheless, the initial release caused a storm as the organization left open the question of how much data was ...to make the underlying data easily available, organized in a form that can be understood by others, balanced...each individual video. To PLOS’s credit, the organization subsequently clarified what the policy intent...researchers. Yet accurate and comprehensible organization of data is essential for efficient science. ...as SlideShare, Figshare and Dataverse. Just as Addgene helps with DNA reagents, these resources can offload...David Crotty (The Scholarly Kitchen) Read more Addgene blog posts about scientific sharing ...

... including the teams at Addgene, ComSciCon, and many more great organizations. At ComSciCon, our primary...your foot in the door Resources on Addgene.org Check out how Addgene uses videos for science communication...respective fields. To help attendees launch new organizations that will extend opportunities to practice science... create them.  Seek out collaborations with organizations that practice science communication or start...entertainment industry.       Additional resources on the Addgene Blog Learn about careers in science communication...

...Protocols Check Out How Addgene Develops Lab Management Software Resources on Addgene.org Find Protoctols ... and do not necessarily represent the views of Addgene. I think it’s safe to assume that anyone reading...me with my research in terms of preparedness, organization, and sharing data. Here are five FREE science...Archives will help both you and your lab stay organized allowing you to upload anything from protocols...came across the Gibco Cell Culture app. Gibco organizes its app into four categories: tools, analytics...100percentSDS.     Additional Resources on the Addgene Blog Learn How to Keep a Lab Notebook for Bioinformatic...

...engineer a specific function in these multicellular organisms. However, the rewards are great if you take the...are needed to increase the body of knowledge. Organizations like Open Plant can then build on this research...plant circadian clock. Petunias have been a model organism for plant research for decades, and the biochemical...limited distribution. We hope that will change with Addgene’s expansion into plant synthetic biology. There ...tool for plant engineering, please share it with Addgene to both build the plant synthetic biology community...on Twitter Like RevBio on Facebook Read more Addgene blog posts about synthetic biology ...

...Regional High School Resources at Addgene  Read more Inside Addgene Blog Posts Get Career Advice from...that Addgenies represent. They kicked off their day with breakfast and an introduction to Addgene with...to start networking! Learning about careers at Addgene Afterwards, the students split into two groups ...groups for an in-depth look at some of the teams at Addgene. An introduction to plasmids with Director of Biology...with the “comfortable surroundings” of the new Addgene office space.  Thanks Abigail, we think it’s pretty...pretty great, too! Thanks to MassBioEd for organizing such a great day, we look forward to hosting more...more students next year! Does your lab or organization do work to help improve K-12 education? Let us ...

...multiplex gene editing in a variety of cells and organisms. However, lengthy genetic payloads and insufficient...processing mechanism, which is conserved in all living organisms and has previously been used for multiplex Cas9...nuclease editing, in virtually any cell type or organisms of interest with only minimal genetic payloads...detailed manual and online design examples of using Addgene plasmids hCtRNA_FT (#186715) and hCtRNA_VT (#186716...References and Resources More resources on the Addgene blog CRISPR 101: Multiplex expression of gRNAs ...

...opportunity. That's how Addgene was founded. That's how my position and my team at Addgene was created.”- Eric...discussed on the Addgene blog, consider writing a guest post. Additional resources on the Addgene blog Read...Resources on Addgene.org Find job listings at Addgene Get more career insight from Addgenies ...downloadable guide: the Addgene Science Career Guide. We also asked Addgenies what their favorite career...planning meetings, and finding the best way to organize your time. At a conference, you could be honing...  We touch upon these topics frequently in the Addgene blog. In fact, we have so many blog posts about...find yourself asking, "Why doesn't this product/organization/position exist yet?", you are in the early stages...

...the more detailed questions the organizers wanted to touch on in organizer David Riglar’s blog post. Essentially... that there are many companies and organizations (including Addgene), that need people who are data oriented...Addgene recently attended and sponsored the Future of Research (FOR) Boston Symposium 2015. I personally...fun and surprisingly exhausting experience. As Addgene believes many of the topics discussed during the...Symposium On the FOR website, the FOR Boston 2015 organizers indicate that the main question they wanted to...skills beyond those required at the lab bench. At Addgene we try to do our part in boosting awareness of ...problems within the research environment itself. At Addgene we hope that all parties involved in making academic...

... work with Drosophila as an experimental model organism. I then described the Gal4/UAS system used by ...various plasmids for overexpression and knockdown. Addgene also has a number of insect vectors to consider...easy way to see what is available is to browse Addgene’s Collection under the insect expression system ...species of gene. Find Gal4 and UAS plasmids at Addgene Choosing a vector to generate a transgenic fly ... survival. For those interested in development, organs of interest, such as the intestines, can be dissected...have!) (4). The power of Drosophila as a model organism is in its amenability to genetic manipulation....PMCID: PMC3172133. Additional Resources on the Addgene Blog Learn about Using Cre in Drosophila Read ...

... al., 2024). They deposited their plasmid with Addgene, hoping to inspire others to explore the phenomenon...and optogenetic tools exist, humans (like many organisms) are opaque, which makes most biomedical applications...There is a variety of research on whether and how organisms respond to magnetic fields (Karki et al., 2021...sometimes seen controversy, and the interactions of organic molecules like proteins with magnetic fields generally...magnetic responses of cryptochrome proteins or organic fluorophores (Karki et al., 2021; Lee et al., 2011...doi.org/10.1038/nn.4265.  Additional resources on addgene.org Find Optogenetics Plasmids Find Biosensor Plasmids...

... can function to break membranes of different organisms. And finally, we demonstrated that R bodies can... wild type and mutant R bodies can be found at Addgene here. What can R bodies do for you? R bodies are...speculatively, R bodies might endow divergent organisms with new functions. For inspiration, we can look...Kanabrocki, J. A., Quackenbush, R. L. & Pond, F. R. Organization and expression of genetic determinants for synthesis...26814170. Find Plasmids from This Publication at Addgene 4. Petroni, G., Spring, S., Schleifer, K.-H., ...Central PMCID: PMC26518. Additional Resources on the Addgene Blog Read About Other Hot Plasmid Technologies...Plasmid Tools for the Repository Resources on Addgene.org Read our Hot Plasmids Articles Find Plasmids...

... Biology Resources on the Addgene Blog Deposit your plasmids with Addgene and make your work more accessible...our days more effective, and our schedules more organized. From the alarm in the morning, to the daily news...note-taking tools, others using excel sheets or organizing folders in complex ways, there are solutions ...institution, the transition is smooth as all data is organized within an ELN already. Investing some time in ...makes it much easier for lab members to manage and organize their work at every step of the research process...Twitter @scinoteELN Additional Resources on the Addgene Blog Learn about more tools for reproducibility...

...scientists to edit the genome of virtually any organisms on this planet, CRISPR-Cas systems were merely... to quickly detect the presence of infectious organisms (ex: SARS-CoV-2) and genetic mutations. By providing...ID PMID: 30337455. Additional resources on the Addgene Blog Learn more about DETECTR Browse all of the...Download our CRISPR cheat sheet Resources on Addgene.org Find the Cas14 plasmids here Checkout our CRISPR...

...’s team devised the plasmids now available in Addgene’s repository for use in neurons, both in culture...tissue. “The brain is such an amazing and dynamic organ,” Konermann explained. “It helps us adapt to influences...use is by no means limited to particular model organisms or cell types. “This tool is really flexible and... Kendall Morgan...

...stakeholders, the product managers collate and organize that information and form it into a roadmap--a... features, programs, or tools to build. After organizing the information, a software product manager works...everything running smoothly, you’ll want to be really organized. Often, product managers will have three or more...twitter @rgrubinstein. Additional resources on the Addgene blog Discover more science career options Check...

...This is the fourth post in the Addgene Blog Mentoring for Scientists Series. I have been thinking a lot...like to start a group mentoring program at your organization, contact me! I can provide a step-by-step, how-to... people from different labs, departments or organizations as it brings a diversity of perspective. Sometimes...transition. Schedule your first meeting and get organized. Discuss your general areas of interest and what...

...sharing Addgene is all about helping scientists share between labs, and people work hard at Addgene to make...Right from the start, when we founded Addgene nearly 10 years ago, lunch felt like a family meal. My ...sports, movies, and just about anything. As our organization grew, we continued this tradition. We now have...as one of their favorite parts about working at Addgene. We’re a small non-profit, so we can only provide...delicious) team building activity that is easy to organize. We’ll dedicate a post to these competitions in... started Hack Days. During Hack Days, teams at Addgene will forgo their regular To-Do lists (other than...Cross-team “Random Lunches” With more employees, Addgene has naturally divided into smaller functional teams...