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We narrowed to 389 results for: ARIA

Showing: 101 - 150 of 389 results

  1. Four Base Editing Reporters to Monitor and Enrich Editing in Real-time

    Type
    Blog Post
    ... et al., 2016). For TREE they engineered a BFP variant that undergoes conversion to GFP after being targeted...changes the histidine to a tyrosine generating a GFP variant (GFPY66) that has a shifted emission spectra. Thus...the test by infecting HEK-293 cells with the BFP variant, a BE4am-Cas9 base editor, and sgRNA vectors for...the Wang and Brafman lab uses an engineered BFP variant that converts to GFP after a base editing event...

  2. Hot Biosensors 2022: Year-End Roundup

    Type
    Blog Post
    ...for synaptic glutamate response. They generated variants optimized for different applications (v82 has ... and powerful high-throughput screen to select variants based on performance metrics specifically for ...BeadScan platform for screening candidate biosensor variants for performance under two-photon fluorescence ...heat with B-gTEMP  Measuring local temperature variations and heat transfer at the subcellular level requires...

  3. Viral Vectors 101: AAV Serotypes and Tissue Tropism

    Type
    Blog Post
    ...shell packages the vector’s genetic material, and variations between serotypes can change how the viral particle...currently understand it. Tropism depends on many variables, so be sure to check the literature for previous...D. V., & Karpova, A. Y. (2016). A Designer AAV Variant Permits Efficient Retrograde Access to Projection...An Introduction to AAV Viral Vectors 101: AAV Variables that Matter Viral Vectors 101: Systemic Capsids...

  4. A Control for All Seasons

    Type
    Blog Post
    ...response recorded would be due to natural biological variation. This information is critical for correctly interpreting...of the same sample and are included to assess variability in the experimental protocol. In the experiment...results vary considerably, then the high degree of variability in the process could make it difficult to obtain...assess naturally occurring random biological variability. If one were studying the effects of tau kinase...

  5. A Primer on Optogenetics: Introduction and Opsin Delivery

    Type
    Blog Post
    ...any optogenetic experiment. There are numerous variants for the different types of opsins available and...cell-type specificity, the kinetics of the opsin variant, and other particularities that best suit your ...these reviews for a discussion of the different variants [1, 2, 4]. And, of course, the best way to learn...learn about the many different opsin variants and how they can be used is to consult the many primary research...

  6. New Viral Vectors - Summer 2024

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    Blog Post
    ...also added AAV1 and AAV5 preparations of several variants of a new optogenetic tool called PdCO, deposited...offering AAV1 and AAV5 preparations of several variants of a new optogenetic tool called PdCO.  This is...Hub What is a Virus (and a Viral Vector)?  AAV Variables that Matter...

  7. The CRISPR Software Matchmaker: A New Tool for Choosing the Best CRISPR Software for Your Needs

    Type
    Blog Post
    ...Pasteur, Paris) that changed genome editing in the malaria community for the better (Nat. Biotechnol., 2014...Plasmodium falciparum (the parasite that causes malaria). I attribute their success to technical expertise...the software piñata has been filled with slight variations on a theme without many resources to review them...Ghorbal, Mehdi, et al. "Genome editing in the human malaria parasite Plasmodium falciparum using the CRISPR-Cas9...

  9. Optogenetics + CRISPR, Using Light to Control Genome Editing

    Type
    Blog Post
    ...photoactivatable repressor (dCas9). The activity of all variants was reversible and repeatable. Since these initial...CRISPR–Cas9 activity switching via a novel optogenetic variant of AcrIIA4 (CASANOVA). As shown in the figure below...Publication System nickname Photoactivatable moiety Cas9 variatn CRISPR protein application Split Systems / Light-induced...Engineering of temperature- and light-switchable Cas9 variants. Nucleic Acids Research 44: . https://doi.org/...

  10. Year of the Camelid: Antibody Style

    Type
    Blog Post
    ...developing transgenic mice that express camelid variable domains (aka nanomice), which gives researchers...modifications made to generate the nanomouse. (VHH = variable domains of heavy chain only antibodies) (B) Nanomice... camelid mice and llamas neutralize SARS-CoV-2 variants. Nature 595:278–282. https://doi.org/10.1038/s41586...

  11. Sensing Neuronal Dopamine

    Type
    Blog Post
    ... sensor. This sensor was engineered from a GFP variant and a DA receptor such that GFP fluoresces when...when the receptor is bound by DA. Two variants of the sensor, dLight1.1 and dLight1.2, exhibit high (submicromolar...GRABDA sensor based on a similar strategy: a GFP variant bound to a DA receptor, such that the GFP fluoresces...

  12. New Viral Vectors - Winter 2025

    Type
    Blog Post
    ...AAV1 Biosensor Marianne Fyhn New viral prep AAV-hSyn-Ribo-jGCaMP8m AAV1 Biosensor Marianne Fyhn New viral...Hub What is a Virus (and a Viral Vector)?  AAV Variables that Matter...

  14. Choosing Your Fluorescent Proteins for Multi-Color Imaging

    Type
    Blog Post
    ... are mTagBFP2, EGFP or one of the improved GFP variants, mRuby2 or TagRFP-T, and an infrared fluorescent...In mammalian cells, one of the improved folding variants of EGFP like mEmerald or Clover is probably best...measurements. Here, EGFP outperforms the improved folding variants, presumably due to the lower growth temperature...

  15. Hot Plasmids - February 2022

    Type
    Blog Post
    ...Here, Liu et al. engineer a chimeric single chain variable fragment (scFv), grafting the binding region of...optimizing enzyme activity using an enhanced Cas12a variant (enAsCas12a), minimizing off-target effects by ... reducing recombination by finding acceptable variations in DR sequences. Figure 2: comparison ...

  16. Luminescent Imaging with Nano-lanterns

    Type
    Blog Post
    ...technology. Nano-lanterns contain a Renilla luciferase variant fused to an FP; when supplied with a luciferase...BRET). Photons emitted by a Renilla luciferase variant are used to excite the fused fluorescent protein...Addgene. Suzuki, Kazushi, et al. “Five colour variants of bright luminescent protein for real-time multicolour...

  17. Behind the scenes: Addgene’s new search engine and more

    Type
    Blog Post
    ...created a normalization mechanism to “collapse” variations and synonyms of the same term that appear in ...promoter “Syn”, that, when selected, will match variations present in the data such as synapsin, hsyn synapsin...synapsin, hSynap. Matching We capture multiple variations of the same information in our search indices...

  18. Targeted Mutagenesis with EvolvR

    Type
    Blog Post
    ...scale by screening diverse libraries of genetic variants. Several methods for generating mutant libraries...provides a new approach for generating novel genetic variants in bacteria. Read on to learn about the key components... other three bases, which limits the level of variation that can be introduced.   Diversification is...

  19. Hot Plasmids - May 2022

    Type
    Blog Post
    ...continuation of that work, they have found at least two variants that display increased neuronal expression in ...side effects, by allowing the user to select for variants that infect the CNS while selecting against those...subclones within a single well reduced well-to-well variability, while the readout was able to be deconvoluted...

  20. CRISPR 101: Cas9 vs. The Other Cas(s)

    Type
    Blog Post
    ...from countless rounds of optimization. Dozens of variants exist with unique cutting features and evolved...engineering endeavors. For more information on Cas9 variants, check out our blog post on choosing a Cas9 enzyme... detection tool for rare, often hard to detect variants such as single nucleotide polymorphisms. Fast...

  23. Antibodies 101: Affinity Reagents

    Type
    Blog Post
    ... are created through enzymatic cleavage of the variable region (the antibody region that binds to the ...immunogenic version is needed.  Single-chain fragment variables (scFvs) are ~27kD engineered fusion proteins similar...instead of cleavage. They are comprised of the variable heavy and light chain regions, connected by a ...

  25. Viral Vectors 101: Producing Your rAAV

    Type
    Blog Post
    ...genome. While ddPCR is superior in precision and variability, qPCR is the more widely used method due to the...Deng, D., Menezes, K., Londono, L., Arjona, K., Parianos, M., Truong, V., Rostonics, E., Hernandez, A.,...Packaging PlasmidParts of the AAV Transfer PlasmidAAV Variables That Matter AAV protocols on addgene.org  AAV ...

  26. Polymerase Chain Reaction Overview and Applications

    Type
    Blog Post
    ...gene expression profiling, studying copy number variation, and molecular diagnostics.  Digital droplet PCR...concentration Gene expression profiling Copy number variations Quantify RNA levels Molecular Diagnostics NGS...of starting material AAV Titrations Allelic Variations Quantifying low abundance targets Monitoring ...

  28. Finding nucleic acids with SHERLOCK and DETECTR

    Type
    Blog Post
    ..., scientists have utilized CRISPR-Cas9 protein variants, Cas13, and Cas12a, to develop simple, portable...targets in one reaction, Cas enzymes, such as variations of Cas13 and Cas12a, are combined with different...CRISPR Trans Reporter Cas12a (Cpf1) is a CRISPR Cas variant that can also cleave dsDNA similarly to Cas9 (Zetsche...

  29. Quick Guide to Near-Infrared Fluorescent Proteins

    Type
    Blog Post
    ...This post was contributed by guest blogger Daria Shcherbakova, a faculty member at Albert Einstein College... experiment!   Thank you to our guest blogger! Daria Shcherbakova is a faculty member at Albert Einstein...optogenetic tools. Follow her recent research @daria_gift_shch.     References and resources References Shemetov...

  30. The Fluorescent Vegetables in Aptamer Soup

    Type
    Blog Post
    ...Gram-positive bacteria (Kellenberger et al., 2013). Other variants of Spinach include Baby Spinach, a miniaturized...short non-coding RNAs (Autour et al., 2018). Three variants of Mango, coined Mango II, III, and IV, were then...high binding affinity to TO1-biotin. Two of these variants exhibit increased brightness compared to Mango...

  31. Antibodies 101: Antibody Engineering and Directed Evolution

    Type
    Blog Post
    ...Antibodies can be broken up into smaller parts, the variable regions can be altered for specific functions,...IgG: immunoglobin G; ScFv: single-chain fragment variable; sdAb: single-domain antibody (often referred ...19px;font-family: helvetica;","color":"#FFFFFF","variant":null,"font":"helvetica","style":"font-family: ...

  32. RNA Interference in Plant Biology: New Tools for an Old Favorite

    Type
    Blog Post
    ...approach are thought to be off-target effects and variable silencing efficiency. To address these shortcomings...screening method was limited in two substantial ways: variable silencing efficiency and labor-intensive processes...sufficient degree remain alive. This likely reduces variability in the RNAi output and trivializes downstream...

  33. Antibodies 101: Validation

    Type
    Blog Post
    ...and specificity. However, there are countless variables that impact an antibody’s affinity and specificity...very similar proteins. Additionally, they require variable expression of your target across samples - it ...it is hard to distinguish between specific, invariable expression and non-specific, background signal, ...

  34. Sequencing Options for CRISPR Genotyping

    Type
    Blog Post
    ... sensitivity Sanger Sequencing Provided 20% (variable) Sequence-level data Low-throughput, inappropriate... Targeted Amplicon Sequencing Provided 0.01% (variable) Sequence-level data, extremely sensitive Do not...sequencing: a comparison of methodologies for KRAS variant detection." Biotechniques 53.3 (2012): 182-188....

  35. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    ...ensures that all screened plasmids contain a gene variant* and is amenable to both high and low throughput... recently developed a mitochondrially-targeted variant of PSmOrange, a photoswitchable fluorescent protein... Osaka University have developed two new color variants of their Nano-lantern technology, first developed...are designed to complement the original yellow variant, with all 3 having distinct emission spectra suitable...whereby photons emitted by a Renilla luciferase variant are used to excite a fused fluorescent protein,...

  36. CRISPR 101: Multiplex Expression of gRNAs

    Type
    Blog Post
    ... with the Gersbach lab plasmids, multiple Cas9 variants are available: wt humanized Cas9, D10A nickase...clone your own entry vectors containing other Cas9 variants to use with the MuLE system. Multiplexing from...Topic Page Get Advice on Choosing The Proper Cas9 Variant for Your Experiment Learn About Multiplexing with...

  38. The 12 Days of CRISPR

    Type
    Blog Post
    ...CRISPR, Addgene gave to me: Functional Genetic Variants Revealed by Massively Parallel Precise Genome ...CRISPR, Addgene gave to me: High-fidelity Cas13 variants for targeted RNA degradation with minimal collateral...

  39. Technical Design of a Western Blot

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    Blog Post
    ... plasmids and have very little batch-to-batch variation. Confirmation of sequence can be done from relatively...antibodies Higher non-specific binding; lot-to-lot variability Some recombinant antibodies Not always ...antibodies Higher non-specific binding; lot-to-lot variability Recombinant multiclonal antibodies Not ...

  40. Editor's Choice, July 2016

    Type
    Blog Post
    ...editing when compared to the most commonly used Cas9 variant, SpCas9. These properties, combined with other ...in the post, may make Cpf1 the preferred CRISPR variant going forward.   Special Delivery Fluorophore Targeting...

  41. Troubleshooting and Optimizing a Western Blot

    Type
    Blog Post
    ...antibodies, which have much lower lot-to-lot variability. If you’re running an already-developed assay...Add sonication step to protein lysis Splice variants Check literature Posttranslational modifications...use a less sensitive exposure kit No signal variation in highly expressed proteins Antibody dilutions...

  42. Your Top Requested Plasmid in 2016!

    Type
    Blog Post
    ...GFP-tagged and un-tagged versions of SpCas9 and further variants replace SpCas9 with SpCas9n (nickase), which nicks...homology directed repair. You can find all of these variants, as well as links to the Zhang Lab's Nature Protocols...

  43. Seeing Red: Simple GFP Photoconversion

    Type
    Blog Post
    ...imaging process worked well with the common GFP variants EGFP, mGFP4-T, and S65T GFP, and no red color ...enthusiasts! Addgene has empty backbones containing GFP variants used by Sattarzadeh et al., as well as a wide ...

  46. Antibodies 101: Fc Effector Functions

    Type
    Blog Post
    ...antibodies in the lab, we focus on a lot on the variable domain and not so much on the constant, or Fc,...Casadevall, A. (2016). Ig Constant Region Effects on Variable Region Structure and Function. Frontiers in Microbiology...

  47. Viral Vectors 101: Parts of the AAV Packaging Plasmid

    Type
    Blog Post
    ...far been identified: AAV1-11. Nine regions of variability in the cap gene which differ between the AAV ...changes and/or short peptides sequences into these variable loops is one method for changing a capsid’s tropism...

  48. Sleeping Beauty Awakens for Genome Engineering

    Type
    Blog Post
    ...Zsuzsanna Izsvak’s lab screened many SB tranposase variants for hyperactivity in mammalian cells. Their efforts...inducible expression vectors were developed into 16 variants with different selection markers (blasticidin,...

  49. Antibodies 101: Avoiding the Mouse on Mouse Mess in IHC

    Type
    Blog Post
    ... antibody that is a Fab fragment, single-chain variable fragment (scFv), or a nanobody is one potential...Affinity Reagents Antibodies 101: Single-Chain Variable Fragment (scFvs) Resources on Addgene.org Recombinant...

  50. New and Upcoming Viral Vectors - December 2019

    Type
    Blog Post
    ... plasmids now packaged in AAVrg. jGCaMP7s, the variant with the most sensitive response to action potentials...to measure glutamate levels. iGluSnFR.A184S, a variant with higher affinity to glutamate, has been packaged...
Showing: 101 - 150 of 389 results