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We narrowed to 208 results for: pme i

Showing: 101 - 150 of 208 results

  1. Finding and Joining Your Dream Lab

    Type
    Blog Post
    ...this PI looking for in a new researcher?,” “How do I convince the PI to choose me?” We asked three leading...how it will be before you take the position. Am I choosing the right mentor? Make sure your advisors...neighbors during the interview process. What if I want a non-academic career path?  If you are considering...source software, hardware, wetware, technology development, etc). Second, the potential hire visits the ...identifying a lab that fits both work style and development needs. These two factors will provide a solid...

  2. Cancer, Inflammation and Immunity - Harnessing the Body’s Defenses to Fight Cancer

    Type
    Blog Post
    ...defeat it we need to attack it from all fronts!”  Here I aim to compile a few highlights from the conference...Methods to study autophagy can be found here. Fun fact: I also learned that Professor Kroemer often functions...from the poster sessions During the poster session I got a chance to talk with Dr. Friedmann-Morvinski ...CAR T cells and PD-1 modulators. An exciting development in immunotherapy is the combination of the CRISPR...PMID: 28225754. 6. Marumoto, Tomotoshi, et al. "Development of a novel mouse glioma model using lentiviral...

  3. Bringing Sustainable Practices to the Lab: Easy Action Items

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    Blog Post
    ...room and don’t have to keep buying more freezers. I am pretty sure that the world will go on without my...the same. Long-term lab conservation goals Lastly, I think it’s a good balance to have both short-term ...reduce your consumption in the lab: Turn off your equipment at the end of the day and when not in use. You...making dinner at night, so why leave your lab equipment on? This applies to water baths, computers, and...

  4. Management for Scientists: What Makes a Good Manager Anyway?

    Type
    Blog Post
    ...the common mode of operating.  Managing scientists I should add a few special words about managing scientists...Biotechnology article “Managing knowledge-intensive workers”. I have added a few comments of my own to this list. ...work, opportunities to pursue and problems to solve (I think this is true of most people, not just scientists...training or teaching others, research, and policy development. Every worker at every level does better with...

  5. Choosing a Good Mentor for Scientists

    Type
    Blog Post
    ...Cepko to learn about her mentoring style. How can I find out if an advisor is a good mentor? You have ... more resources to help you choose a good mentor, I have provided a downloadable PDF at the end of this...scientists think, “Oh, but that won’t happen to me – I am different.”  Unfortunately, a bad mentor can be...practice successful grant writing, lab budgeting, equipment acquisition, public speaking and personnel management...

  6. Sharing is Caring: But How to Distribute Open Hardware?

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    Blog Post
    ... Open source hardware builds   Since I started my PhD, I have been convinced that most microscopy ...a number of new platforms (such as openUC2, which I am a co-founder of), that aim to support the community...taking these limitations into account in the development process. Widely used, commercially available ...Squid: Simplifying Quantitative Imaging Platform Development and Deployment." bioRxiv. 2020. Diederich, B....

  7. 5 Uncommon Ways to Gain Career Skills During an Uncommon Time

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    Blog Post
    ...   3. Start something In almost every career talk I present, I’m asked what Addgene “looks for” when hiring... The online dictionary gives two definitions that I like: “the ability to assess and initiate things independently...start? 4. Do informational interviews (on a schedule) I hope you all know by now that people get jobs at least...wait for it to be over to work on career skills development. Back in March, we posted these 25 great tips...

  8. Negative Can Be Positive: Open AAV Data with Addgene

    Type
    Blog Post
    ...consider my first challenge when I started working with AAV vectors. I wanted to use an AAV vector to express...effort to test and optimize our vector - overall, I spent two years testing and reformulating our AAV ...from people trying to answer the exact same question I was: What AAV vector is best for my specific system...Vectors. Molecular Therapy - Methods and Clinical Development, 18(September), 98–118. https://doi.org/10.1016...

  9. Using CRISPR/Cas9 to Edit Disease Out of the Genome

    Type
    Blog Post
    ...editing techniques is its ease of use.  “I was really excited when I saw these two papers,” said the University...conditions. Remaining hurdles in CRISPR biology “I think it is absolutely possible to use CRISPR to cure... It is speeding the process of the discovery/development of genetic targets and RNA therapies. [6]. In...

  10. New CRISPR Web Resources and #12DaysofCRISPR Recap

    Type
    Blog Post
    ... able to edit RNA in mammalian cells with CRISPR? I certainly didn’t, but now we can with Cas13b/ADAR ...from the REPAIR (RNA Editing for Programmable A to I Replacement) system. Like RNA targeting systems, RNA... to make it easy for you to keep up with new developments (and, of course, find plasmids that will be ...CRISPR History provide a great overview of the development of CRISPR into a varied research toolbox, as ...Plasmids and Resources and CRISPR History and Development for Genome Engineering  ...

  11. New CRISPR Tools: Cas7-11 and PASTE

    Type
    Blog Post
    ...phylogenetically classified as the more complex Class I systems that contain multiple subunits, but the more...its ancestors. They classified the system as Class I, Type III-E, and then used this information to identify...03886-5. Epub 2021 Sep 6. PMID: 34489594. Eleonora I. Ioannidi, Matthew T. N. Yarnall, Cian Schmitt-Ulms...and cellular biology, this happens through the development of tools that exploit and expand our current ...which we're featuring in today's blog. Cas7-11 Development and Discovery As part of their quest to find,...Cas systems opens up the potential of further development of low-toxicity RNA editing tools.   Fig...embryonic stem cells and glioblastoma cells. PASTE Development and discovery The first generation of CRISPR ...

  12. New Tools Enable CRISPRa for Neuroscience Applications

    Type
    Blog Post
    ...CRISPRa, the Day lab singled out two Bdnf isoforms (I and IV) for investigation. Co-transduction of primary...hundreds of differentially expressed genes between Bdnf I-overexpressing neurons and Bdnf IV-overexpressing ...Among the upregulated genes common between the Bdnf I and Bdnf IV overexpression variants were other immediate-early...University of Rochester Medical Center. During development, complex genetic programs specify and assemble...technological gap, a trio of recent papers describe the development of CRISPR activation (CRISPRa) tools for neuroscience...recently circumvented by the Yang Lab with the development of a Suntag-p65-HSF1 (SPH) transgenic mouse (...regulates neuronal physiology by promoting the development of neurons and synapses as well as long-term ...

  13. Sonic Hedgehog? Sleeping Beauty? Learn About the Genes Behind Addgene’s Conference Room Names

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    Blog Post
    ...protein after a vegetable substitute for spaghetti? I actually couldn’t find the specific origins of the...does not form properly (Azplazu and Frasch, 1993). I could not find the exact origin of this gene name ...contains bagpipe from the Deplancke lab. Conclusion I hope you enjoyed this tour of Addgene’s new conference...primarily involved in development. These genes are known to play a role in neural development, sex determination... a transcriptional co-repressor important in development. Groucho family proteins are found in flies, ...Hedgehog, an important regulator of embryonic development The largest conference room at Addgene is named...rather to an extremely important gene in animal development. To understand where sonic hedgehog got its name...

  14. Google Forums Round Up: First Impressions of NgAgo

    Type
    Blog Post
    ...how does NgAgo stack up thus far? A few weeks ago, I was going through NgAgo google forums where people... dig more into what researchers felt about NgAgo, I conducted a survey and asked researchers if they had...people are having difficulty with the NgAgo system. I encourage you to share your results and protocols ...More time is required for its optimization and development before it can truly be pitted against CRISPR ...Patrick D., Eric S. Lander, and Feng Zhang. "Development and applications of CRISPR-Cas9 for genome engineering...

  15. Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection

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    Blog Post
    ...invisible information into actionable signals. Let's say I wanted to detect every primary metabolite in a cell...sensor domains for everything you could imagine. That I think is the next priority; we need the keyboards ... programming language is put into practice? Endy: I think it's important to understand that these platforms...with a publication in Science describing the development of “transcriptors,” transistor-like digital genetic...engineered RNA switches, but we've not yet seen the development of enough sensor domains. Ultimately, we'll need...

  16. Plasmids 101: Cre-lox

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    Blog Post
    ...translocation event is generated at the loxP sites. How can I use Cre-lox? The Cre/lox system is a well-established...successfully introduce Cre into cells of interest. What if I need two separate recombination events? One potential... 1. Bacteriophage P1 site-specific recombination. I. Recombination between loxP sites. Sternberg, N. and...cell or tissue types, during certain stages of development, or by making the Cre inducible (such as with...

  17. Twenty Years of Sharing: Expanding Accessibility

    Type
    Blog Post
    ...stopping by to say, “Everything I know about virus, I learned from Addgene,” or “I learned to clone from the...materials, we noticed a large percentage of these shipments arrived in India at room temperature. Partnering...distributor for that country. In China, direct shipments weren’t able to clear import control. Partnering...

  18. A Primer on Optogenetics: Introduction and Opsin Delivery

    Type
    Blog Post
    ... and experimental limitations and considerations? I recently spent two months in the lab of Antonello ...behavioral experiments. I’m not an electrophysiologist so I cannot speak directly on this topic but, in numerous...neural pathways involved in addiction (such as the lab I work in), an obvious behavioral experiment to test...injections until they are accurate and reproducible. I also highly recommend spending the money on a high... one of the key scientists who pioneered the development of optogenetics, has genetically manipulated ...nrn3171. PMID: 22430017. 2. Fenno L, et al. The development and application of optogenetics. Annual review...

  19. FPbase: A new community-editable fluorescent protein database

    Type
    Blog Post
    ...effort to collate some of the current “best” FPs, and I collaborated with him on a web-based interactive visualization...FP spectra (and rapidly growing, with your help!), I believe this represents one of the largest available...for future development of FPbase FPbase is still very much in the early stages of development, and there...

  20. Of Myc and Men

    Type
    Blog Post
    ...step “...proved to be quite easy to achieve…” - but I think we can all agree that it sounds less than ideal...9E10 antibody remains a trusty antibody partner; as I write this, there are close to 10,000 citations for...0092-8674(87)90086-9  Schüchner S, Behm C, Mudrak I, Ogris E (2020) The Myc tag monoclonal antibody 9E10... mysterious, microscopic world of cells. The development of antibodies as tools allowed us to detect and...

  21. Dos & Don'ts When Publishing a Scientific Manuscript

    Type
    Blog Post
    ...writing up your research? What type of journal should I choose? Writing a research paper for publication in...to include in your manuscript. What information do I need to include in my paper? More and more journals...post was contributed by Maaike Pols, PhD, a Developmental Editor at F1000Research.  With many changes ...figures and results. This is a relatively new development, and it allows reviewers and other researchers...You to Our Guest Blogger! Maaike Pols is a Developmental Editor for F1000Research, responsible for assessing...

  22. Supporting Rare Disease Research with the 2020 BeHEARD Awards

    Type
    Blog Post
    ..., and Dr. Angela Lin got me interested in TS when I was a newly independent faculty member studying sex...partners can regulate ion channels in the brain.  “I have a long standing interest in the biology of GPCRs...Deficiency Syndromes (CCDS) that present as global developmental delays or autism spectrum disorder. It is estimated...creatine deficiency, which is characterized by development delays, speech delays, seizures, and other symptoms... enhance our ability towards CTD therapeutic development,” says Iyer. Since the RESOLUTE plasmid collection...Though there are IL-36 receptor inhibitors in development, there’s still a lot of mysteries surrounding...in Turner syndrome Turner syndrome (TS) is a developmental disorder where one of the X chromosomes is partially...

  23. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

    Type
    Blog Post
    ...efficiencies in high-throughput libraries My labmates and I wanted to find a way to predict the efficiencies of... Weller, J., Peets, E. M., Pallaseni, A., Kuzmin, I., Raudvere, U., Peterson, H., Liberante, F. G., & ...Damodharan, T., Balázs, Z., Janjuha, S., Ioannidi, E. I., Böck, D., Steensel, B. van, Krauthammer, M., & Schwank... Allam. Continued training led to the recent development of PRIDICT2.0 (Mathis et al., 2024). The additional...

  24. A Deep Dive into BioBuilder

    Type
    Blog Post
    ...of students and teachers in the U.S. and beyond. “I want to learn more about Synthetic Biology. Can you...students are asking. Teachers are also asking, “How do I engage my students in real-world lab activities? I...and collaboration. One module currently under development comes from the Caltech lab of Professor Richard... internships. A summer teacher professional development program that trains nearly 100 teachers each ...

  25. AAV Q&A with Tim Miles

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    Blog Post
    ...AAVs that have successful transduction in microglia? I have been using the AAV.PHP.eb for neuron, oligodendrocyte...immune suppression to allow AAV re-administration but I do not think the field has settled on ‘best practices...Re-administration is a challenge for AAV due to the development of neutralizing antibodies in animal models. ...design in remarkably creative ways. The continued development of more and smaller CRISPR tools is an exciting...

  26. Year of the Camelid: Antibody Style

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    Blog Post
    ...the UN describes. In honor of the Camelid family, I wanted to take a moment to revisit some of the ways...discovery of heavy-chain only antibodies in a camel. And I suspect we will only continue to see their impact ...sdAbs come from? In the early years of sdAbs development, the process started with immunizing a camelid...

  27. Management for Scientists: Delegating is Key

    Type
    Blog Post
    ... for failure by delegating is a bad idea. How do I make sure it goes well? As with most managerial responsibilities...deadlines. Agree on a way to review project progress. I recommend putting a regular check-in meeting on the...Goals with Your Mentor through an Individual Development Plan Resources on the Addgene Website Are You...

  28. Open Resources and Plasmid Tools For Studying C. elegans

    Type
    Blog Post
    ...all over the world. When I was at the 22nd International C. elegans meeting, I was again reminded of the...zebrafish articles published there as well.   Worm Development Dynamics Database (WDDD) The WDDD aims to acquire...computational methods to better understand animal development (Kyoda et al., 2012).  C. elegans plasmid tools...PMC6576255. Kyoda, Koji, et al. "WDDD: worm developmental dynamics database." Nucleic acids research 41...conditional protein depletion in C. elegans." Development 142.24 (2015): 4374-4384. PubMed PMID: 26552885...

  29. Multiplex Genome Editing with CRISPR-Cpf1

    Type
    Blog Post
    ...Zetsche, B., Gootenberg, J., Abudayyeh, O., Slaymaker, I., Makarova, K., Essletzbichler, P.,  Zhang, F. (2015... New Tool for CRISPR Genome Editing Which Cas9 Do I Choose for My CRISPR Experiment A Match Made in Heaven...Note: Cpf1 is also called Cas12a. There’s a new development for CRISPR-Cpf1 genome editing!  A recent paper...editing with CRISPR-Cpf1 is one of the latest developments in CRISPR technologies.  It’s a simple and effective...

  32. AAV Production in HEK293 Cells

    Type
    Protocol
    ...your gene of interest Triton X-100 Benzonase/DNAse I (Millipore 71205-3) 40% Polyethylene Glycol 8000 (...am): Transfect cells Day 7 (am): Harvest cells Equipment Class II, Type A2 Biological Safety Cabinet 0.5...

  33. Quick Guide to Working with Drosophila Part 3: Genome Engineering in Flies

    Type
    Blog Post
    ...with Drosophila as an experimental model organism. I then described the Gal4/UAS system used by geneticists...transformed bacteria, but you might be wondering, “How do I know which of my flies have the desired integration...as basic as survival. For those interested in development, organs of interest, such as the intestines, ...

  34. A Practical Approach to Choosing the B(right)est Fluorescent Protein

    Type
    Blog Post
    ...that will be treated in this series of posts are (i) brightness, (ii) photostability and (iii) aggregation...the Addgene Blog Which Fluorescent Protein Should I Use? Choosing Your Fluorescent Proteins for Multicolor...check all possible combinations of biology and equipment. Because the performance of a fluorescent protein...

  35. Antibodies 101: Secondary Antibodies

    Type
    Blog Post
    ...And while I have it on good authority that a complete Western blot can be run in a single day, I really ... while providing needed flexibility in assay development. Researchers who understand how and why to use...

  36. Save Time with Transient Plant Leaf Transformations

    Type
    Blog Post
    ...pSB96 - pL2_pSB90_2x35S::fLUC-I::tNOS & pSB166 - pL2_pSB90_tMAS::rLUC-I::pMAS. Fluorescent proteins (FP...specific cell types are missing in roots. The development of transgenic hairy roots takes at least six ...scientists do transient leaf transformations The development of transgenic plants is a common problem in non-model...might provide some useful guidelines for the development of transient transformation systems for other...

  37. CRISPR 101: Cas9 vs. The Other Cas(s)

    Type
    Blog Post
    ...Deletions in Human Embryonic Stem Cells Using Type I CRISPR-Cas. Molecular Cell, 74(5): 936-950.e5 (2019... on the Addgene Blog CRISPR 101: Which Cas9 do I choose for my CRISPR experiment? The PAM requirement...can read about Cas7-11 in our blog post on its development and use. Fast facts – PAM requirement: protospacer...

  38. Lab Automation at Addgene

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    Blog Post
    ...find out how you can get one. As a starting point, I would recommend conferences like Lab Robotic Interest...electronic but it is one of the most helpful machines I have used in the lab. It’s especially useful if you...DNA as opposed to in live bacteria. For these shipments, we’ve needed to find a way to purify DNA from...

  39. Corralling and Cornering the Goldilocks Antibody

    Type
    Blog Post
    ... work for your application? Like many scientists, I have spent hours sifting through comments and papers...is a public service to the science community, and I am excited to be a part of it! If you are interested... a wrong section. The antibody screening and development of a focused reference bring us one step closer...

  40. Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards

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    Blog Post
    ... vector that encodes both Cas9 and sgRNA. What if I want to work with gene drives? Then do so! It's a ...Suzuki, D. T. Genetic Control of Insect Populations: I. Cage Studies of Chromosome Replacement by Compound...contributed by Kevin Esvelt, a Wyss Technology Development Fellow at the Wyss Institute and Harvard Medical...

  41. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    ...101: An Introduction to AAV References Asencor, A. I., Dvoryanchikov, G., Makhoul, V., Tsoulfas, P., & ...., Keast, J. R., Miller, C. T., Fox, A. S., Chiu, I. M., & Gradinaru, V. (2022). Engineered AAVs for non-invasive....ymthe.2019.02.013 Huang, Q., Chan, K. Y., Tobey, I. G., Chan, Y. A., Poterba, T., Boutros, C. L., Balazs...Azari, B., Zheng, Q., Beddow, T., Zhao, B., Tobey, I. G., Moncada-Reid, C., Eid, F.-E., Walkey, C. J., ..., J., Zheng, Q., Kozareva, V., Beddow, T., Tobey, I. G., Pacouret, S., Chen, A. T., Chan, Y. A., Ryvkin...Brown, A. R., Shin, N., Kim, Y., Toong, N., Kaplow, I. M., Wirthlin, M., Zhang, X., Phan, B. N., Fox, G.... L., Krunnfusz, A., King, E. M., Ye, S., Solomon, I. H., Tabebordbar, M., & Sabeti, P. C. (2023). Systemic...

  42. xCas9: Engineering a CRISPR Variant with PAM Flexibility

    Type
    Blog Post
    ... be flat out better than SpCas9. I want everyone to test it because I want to know the answer", says Liu...changes compared to SpCas9 in parentheses. The development of xCas9 3.6 and 3.7 shows that we have only ...

  43. Important Considerations in Optogenetics Behavioral Experiments

    Type
    Blog Post
    ...-stimulation (ICSS) and place preference. The lab I work in (the Kreek lab) focuses on the neurobiology...opsins." Nature methods 9.2 (2012): 159-172.5. Goshen I, et al. Dynamics of retrieval strategies for remote...high-light sensitivity optical neural silencer: development and application to optogenetic control of non-human...enlightenment age for zebrafish neurobiology." Developmental neurobiology 72.3 (2012): 404-414. PubMed PMID...

  44. Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

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    Blog Post
    ...technologies are still being developed today. In this post, I will highlight the evolution of the  Brainbow multicolor...Rotkopf S, Alexandre C, Bell DM, Dickson BJ, Salecker I. Nature Methods. 2011 Mar;8(3):260-6. doi: 10.1038... were very time-intensive, necessitating the development of a new technique. In a structure as tightly...lineage tracing; “Zebrabow” was used to trace the development of the corneal epithelium. For scientists interested...interested in neuroscience and development, Brainbow is a valuable tool to mark and follow single cells... Ciruna B, Sanes JR, Luchtman JW, Schier AF. Development. 2013 Jul;140(13):2835-46. doi: 10.1242/dev.094631...

  45. Which Fluorescence Microscopy Technique is Best for Me?

    Type
    Blog Post
    ...should I use for my experiment? When I am contemplating which microscope to use for a new sample I always...can be used to overcome this (8). The recent development of tissue clearing techniques (9) now allows ...

  46. New Optogenetic Tools for Cytoskeleton and Membrane Control

    Type
    Blog Post
    ...journal.pgen.1010765.  Berlew, E. E., Yamada, K., Kuznetsov, I. A., Rand, E. A., Ochs, C. C., Jaber, Z., Gardner,....7554/eLife.84364.  Meiring, J. C. M., Grigoriev, I., Nijenhuis, W., Kapitein, L. C., & Akhmanova, A. ...critical when, say, the gene is essential during development and a knockout is not feasible or when the protein...Rab proteins during a key stage of zebrafish development (Aljiboury et al., 2023). Rab family proteins...formation during zebrafish left-right organizer development. PLoS Genetics, 19(5), e1010765. https://doi....

  47. Great Results Start with Great Standard Curves

    Type
    Blog Post
    ... the concentration of the unknown sample.  How do I make the standards for a standard curve? You create...should be within 15% of the expected value. How do I plot and use a standard curve? To create a standard...depending on a number of factors, including the user, equipment, assay incubation time, etc., which can vary even...

  48. The Challenges of Cell Culture

    Type
    Blog Post
    ...the American Type Culture Collection aka ATCC where I work. It is virtually impossible to work in cell culture...virology without acquiring materials from this bank. I am often asked, “What is the difference between materials...backup liquid nitrogen store to prevent loss from equipment malfunctions. One Master vial can then be thawed...

  49. CRISPR 101: Cytosine and Adenine Base Editors

    Type
    Blog Post
    ...Gaudelli et al., 2017 Adenine base editors (ABE7.10)   A→I (A→G) editing with high product purity and low off-target..., H. A., Packer, M. S., Badran, A. H., Bryson, D. I., & Liu, D. R. (2017). Programmable base editing of...Murray, R., Packer, M. S., Rinaldi, C., Slaymaker, I. M., Yen, J., Young, L. E., & Ciaramella, G. (2020...elements were the starting point towards the development of the first cytosine base editors from Alexis... base editing scope and efficiency Since the development of BE3, many research groups have made improvements...guanine (Thuronyi et al., 2019). Following the development of adenine base editors (discussed next!), RNA...constrained by PAM availability in the genome. So the development of near-PAMless Cas9 variants significantly expands...

  50. Addgene's Top Blog Posts from 2020

    Type
    Blog Post
    ... Store Your Plasmid DNA? by Paolo Colombi Should I store my plasmid in TE, Tris, or water? And what about...blog post with creative ways to continue career development virtually. 6. Quantifying DNA? Here are Five...
Showing: 101 - 150 of 208 results