We narrowed to 84 results for: ALP
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TypeBlog Post... We now have another model of Cas9 – a flexible alpha-carbon backbone model made of nylon – that more ...
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New Tools Enable CRISPRa for Neuroscience Applications
TypeBlog Post...promoters, including calmodulin-dependent kinase II alpha (aCaMKII; excitatory neurons) and Synapsin-1 (pan-neuronal... -
Plasmids 101: Stringent Regulation of Replication
TypeBlog Post... Mizobuchi, K. (1998). Copy number control of IncIalpha plasmid ColIb-P9 by competition between pseudoknot... -
Data Freedom: The Expansion of Data Sharing in Research Publications
TypeBlog Post...diseases. His spare time is spent trying to raise the alpha isoform of GSK-3 to the same (deserved) stature ... -
Plasmids 101: The Promoter Region – Let's Go!
TypeBlog Post...mammalian expression from human elongation factor 1 alpha Constitutive Tends to give consistent expression... -
Fluorescent Proteins 101: When GFP lets you down
TypeBlog Post...successfully generated several functional heterotrimeric G-alpha subunit fusions that do not tolerate N- and C-terminal... -
Hot Plasmids: Summer 2024
TypeBlog Post...peptides (3HB: 11-nm 3-helix bundle; SAH: 60-nm single alpha helix) and target capture module (SpyTag-GFP nanobody... -
CRISPR 101: Epigenetics and Editing the Epigenome
TypeBlog Post...Transcriptional repression DNA Methyltransferase 3 Alpha (DNMT3A) Vlatka Zoldoš’ lab has deposited pdCas9... -
Plasmids 101: Repressible Promoters
TypeBlog Post...expression system for sphingomonads and other Alphaproteobacteria. Appl Environ Microbiol. 2013 Nov;79(21):... -
Plasmids 101: Restriction Cloning
TypeBlog Post...ligation reaction into competent cells such as DH5alpha or TOP10. If using much less total DNA (<1ng) ... -
Mycoplasma Contamination: Where Does It Come From and How to Prevent It
TypeBlog Post...human speech near the laminar airflow, the human scalp, sneezing, coughing, etc. Also, a constant in-flow... -
Lambda Red: A Homologous Recombination-based Technique for Genetic Engineering
TypeBlog Post...Recombineering, EcoSal Plus 2016. doi:10.1128/ecosalplus. PubMed PMID: 27223821. 4. Sharan SK, Thomason... -
Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2: Reprogramming and Transdifferentiation
TypeBlog Post...Thorel, F., et al., Conversion of adult pancreatic alpha-cells to beta-cells after extreme beta-cell loss... -
Immunology Research Plasmids and Resources
TypeCollection...MGC126714, MIP-4a, MIP-4alpha, SCYA26, TSC-1 CCL27 chemokine (C-C motif) ligand 27 ALP, CTACK, CTAK, ESKINE...interferon, alpha 10 MGC119878, MGC119879 IFNA13 interferon, alpha 13 - IFNA14 interferon, alpha 14 LEIF2H...interferon, alpha 16 - IFNA17 interferon, alpha 17 IFNA, INFA, LEIF2C1 IFNA2 interferon, alpha 2 IFNA, INFA2...IFNA5 interferon, alpha 5 INFA5 IFNA6 interferon, alpha 6 - IFNA7 interferon, alpha 7 IFNA-J IFNA8 interferon...activator subunit 1 (PA28 alpha) IFI5111, MGC8628, PA28A, PA28alpha, REGalpha PSME1 proteasome (prosome...activator subunit 1 (PA28 alpha) IFI5111, MGC8628, PA28A, PA28alpha, REGalpha PSME2 proteasome (prosome... -
Trimmer Lab NeuroMab Collection
TypeCollection...-GABA(A)R, Alpha4 [N398A/34R] GABA(A)R, Alpha4 Rat Mouse IgG2a 177544 Anti-GABA(A)R, Alpha2 [N399/19R]...GABA(A)R, Alpha4 scFv [N398A/34] N398A/34 scFv GABA(A)R, Alpha4 Rat Mouse 190552 GABA(A)R, Alpha2 scFv [N399...Mouse IgG2a 128634 Anti-Slo1/BKAlpha maxi-K+ channel [L6/23R] Slo1/BKAlpha maxi-K+ channel Mouse Mouse ...Mouse IgG2a 149456 Anti-GABA(A)R, Alpha5 [N415/24R] GABA(A)R, Alpha5 Human Mouse IgG2a 149458 Anti-Cavbeta2...Mouse IgG2a 164155 Anti-GABA(A)R, alpha6 [N229A/32R] GABA(A)R, alpha6 Mouse Mouse IgG2a 164156 Anti-Gamma-protocadherin-C3...Mouse IgG2a 177486 Anti-Slo1/BKAlpha maxi-K+ channel [L6/60R] Slo1/BKAlpha maxi-K+ channel Mouse Mouse ...Mouse IgG2a 177574 Anti-GABA(A)R, Alpha1 [N95/35R] GABA(A)R, Alpha1 Human Mouse IgG2a 177575 Anti-Collybistin... -
Protocol - pLKO.1 – TRC Cloning Vector
TypeProtocol...NEB #M0202S T4 DNA ligase buffer NEB #B0202S DH5 alpha competent cells Invitrogen #18258-012 Qiaquick gel...Transform 2 μL of ligation mix into 25 μL competent DH5 alpha cells, following manufacturer’s protocol. Plate ... -
Fluorescent Protein Guide: Subcellular Localization
TypeCollection...Microtubules alpha-tubulin mTurquoise2 Dorus Gadella 85045 pmScarlet_alphaTubulin_C1 Microtubules alpha-tubulin...Microtubules alpha-tubulin mCherry* Michael Davidson 12298 pIRESneo-EGFP-alpha Tubulin Microtubules alpha-tubulin...EGFP Anthony Brown 11908 pEGFP-N1 alpha-actinin 1 Actin Filaments alpha-actinin 1 EGFP Carol Otey 27676 ...mScarlet Dorus Gadella 85046 pmScarlet-H_alphaTubulin_C1 Microtubules alpha-tubulin mScarlet-H Dorus Gadella...Gadella 85047 pmScarlet-i_alphaTubulin_C1 Microtubules alpha-tubulin mScarlet-I Dorus Gadella 85054 pLifeAct_mScarlet_N1...mGold Francois St-Pierre 49149 mCh-alpha-tubulin Microtubules alpha-tubulin mCherry Gia Voeltz 89472 GFP-hChibby1...Beta-catenin EGFP Alpha Yap 67937 mouse E-cadherin GFP (423) Adherens Junctions E-cadherin EGFP Alpha Yap 71366... -
Neurodegeneration Plasmid Collection
TypeCollection..., V5 EF-1 alpha Parkinson's Mark Cookson 25081 pDEST51-LRRK2-R1441C LRRK2 His, V5 EF-1 alpha Parkinson's..., V5 EF-1 alpha Parkinson's Mark Cookson 25083 pDEST51-LRRK2-R1441H LRRK2 His, V5 EF-1 alpha Parkinson's..., V5 EF-1 alpha Parkinson's Mark Cookson 29398 pDEST51-LRRK2-R1398Q LRRK2 His, V5 EF-1 alpha Parkinson's..., V5 EF-1 alpha Parkinson's Mark Cookson 29400 pDEST51-LRRK2-T1343G LRRK2 His, V5 EF-1 alpha Parkinson's...30147 pCAX APPs-695-alpha APP CAG Alzheimer's Dennis Selkoe 30149 pCAX APPs-751-alpha APP CAG Alzheimer'...40822 EGFP-alphasynuclein-WT SNCA GFP CMV Parkinson's David Rubinsztein 40823 EGFP-alphasynuclein-A53T SNCA... pHM6-alphasynuclein-WT SNCA His, HA CMV Parkinson's David Rubinsztein 40825 pHM6-alphasynuclein-A53T ... -
Lentivirus Plasmids
TypeCollection...cloned into the plasmid. Nolan 12257 pWPXL 2nd EF-1alpha driven constitutive transgene expression, contains...gives you high expression. Trono 12254 pWPI 2nd EF-1alpha driven constitutive transgene expression and EGFP...EGFP coexpression. Trono 17619 EF.CMV.RFP 2nd EF-1 alpha promoter for transgene and CMV drives expression...similar plasmids. Elledge 21373 pHIV-EGFP 3rd EF-1alpha driven expression of cDNA and and EGFP co-expression... -
Allen Institute for Cell Science Plasmid Collection
TypeCollection...Matrix Adhesions 87421 TUBA1B-mEGFP AICSDP-4 mEGFP Alpha-tubulin Microtubules 87422 LMNB1-mEGFP AICSDP-10...Peroxisomes 101785 TUBA1B-mTagRFP-T AICSDP-28 mTagRFP-T Alpha-tubulin Microtubules 101786 ST6GAL1-mEGFP AICSDP...Transcription Factor 124607 ACTN2-mEGFP AICSDP-63 mEGFP Alpha-actinin-2 Sarcomeric z-disks 124608 NPM1-mTagRFP-T...