...terminal repeat (ITR) sequence Inverted terminal repeats (ITRs) are what makes an AAV transfer plasmid an...(2019) A User’s Guide to the Inverted Terminal Repeats of Adeno-Associated Virus. Human Gene Therapy Methods...

... and human cell lines (Nishimura et al., 2009, Natsume et al., 2016).  The Kanemaki lab previously developed...conditional alleles of a few essential proteins (Natsume et al., 2016). Now, the Kanemaki lab has created...

...with the content we store in them: music, emails, chats, pictures, and apps. Throughout my time as a graduate...universally useful. 4. Chemical Safety Data Sheets by ThatsMyStapler Safety always comes first with research! It...

...this promoter-gene combo are two long terminal repeats (LTRs). These LTRs define the limit of what will...constructs. Sandwiched between two inverted terminal repeats (ITRs) is the promoter and cargo, in that order...

...informal settings like lab meetings, Twitter, coffee chats with potential collaborators, blogging and more....more. These each have different goals, formats, and processes, and they provide different amounts of access...

...viral handling. Gloves are always required and lab coats are either recommended or required in BSL-1 and ...require or recommend disposable gowns over your lab coats and double gloving, so that, if needed, a layer ...

...Monomeric StayGold variants By Mike Lacy In 2022, Atsushi Miyawaki and colleagues identified a highly bright...endothelial cells across diverse mice strains and rats. Figure from Chen et al (2023) under CC-BY license...

...instances I felt like I was going back to previous formats but I learnt to overcome the personal frustration...myself as a scientist. I learnt to wear different hats and came to understand the responsibilities that...

...fluorophores (Karki et al., 2021; Lee et al., 2011; Matsuoka et al., 2023), but these effects were very small...5367–5372. https://doi.org/10.1021/nl202950h.  Matsuoka, R., Kimura, S., Miura, T., Ikoma, T., & Kusamoto...

...pancreatic differentiation) in mouse embryos (Miyatsuka et al.) Distinguish between models for trafficking....2 (2009): 118-126. PubMed PMID: 19136976. 5. Miyatsuka, Takeshi, Zhongmei Li, and Michael S. German. ...

...persistent transgene expression in certain cell types (Tatsis et al., 2007), potentially because some of the ...doi.org/10.1038/s41586-023-05949-1. PMID: 36996871. Tatsis, N., Fitzgerald, J. C., Reyes-Sandoval, A., Harris-McCoy...

... Can be distributed in digital or analog media formats. Cognitively demanding format. Equipment, expense...incorporate visuals if needed. Traditional media formats are quite competitive while digital can be oversaturated...

...causes a shift towards antidepressant behavior in rats in the forced swim test as seen by increasing the...hippocampus evokes antidepressants-like effects in rats." Psychoneuroendocrinology 46 (2014): 78-87. PubMed...

...second-order structure of the AAV genome, caused by repeats and self-complementarity of the AAV genome and ...binding sites between the primers and the AAV genome repeats. Primer annealing can be affected by the presence...

..., a pooled library of scFvs is displayed on the coats of bacteriophages, a process which links genotype...pooled library of scFvs that are displayed on the coats of bacteriophages. This library is incubating with...

...indeed the culprit in egg white injury by feeding rats purified avidin (Kresege et al., 2004). Avidin is...de, Rodriguez, P., Bonte, E., Krijgsveld, J., Katsantoni, E., Heck, A., … Strouboulis, J. “Efficient biotinylation...

...do identify as Democrats more often than Republicans, the March is not about Democrats or Republicans ...

...large inserts (>7 kb)(Berg et al., 1989), direct repeats (Ribeiro et al., 2009), and at high copy numbers...plasmid recombination mediated by 28 bp direct repeats. Mol Biotechnol. 2008 Nov;40(3):252-60. doi: 10.1007...

...Academic Mentors.  What is workplace bullying? Threats to professional status – including belittling opinions...intimidating use of discipline or competence procedures Threats to personal standing – including undermining personal...

...stop codon) and closed (including a stop codon) formats. The collection is compatible with both standard...kit (GGT) that streamlines the assembly of TALE repeats into a single-tube, 3 day process by utilizing ...hard-to-study genes in hard-to-study cell types. Natsume T, et al. Cell Rep. 2016. PubMed PMID: 27052166...