We narrowed to 217 results for: Apple fluorescent protein

...a green fluorescent protein, a red fluorescent protein, a fancy new green fluorescent protein, some affinity...different proteins of interest (or one self-labeling tag and a fluorescent protein) enables applications like...new-and-improved fluorescent protein you want to try, just swap in a new fluorescent ligand and see the...provide many advantages over fluorescent proteins, opening up new applications.   Figure 1: Live-...plasmids containing the same protein of interest tagged with different fusion proteins for different experiments...tags, another version with that same red fluorescent protein somebody made because they forgot it was ...Just express the fusion protein and add a specific ligand! A self-labeling protein gives you the flexibility...

...Photoactivatable Fluorescent Proteins Fluorescent Protein Timers Return to top Protein Interactions Förster...Strains for Protein Expression Protein Expression Epitope Tags Protein Tags Return to top Protein Visualization... Split Fluorescent Proteins for Studying Protein–Protein Interactions Return to top Controlled Expression...that can be used for protein purification, protein visualization, study of protein interactions, controlled...Bacterial Expression Systems Protein Purification Protein Visualization Protein Interactions Controlled Expression...plasmids that contain fluorescent protein (FP) tags so you can express your protein of interest fused to... Additional Addgene Protein Visualization Resources Visit our Fluorescent Protein Guide to find an extensive...

...to label loci, such as fluorescence in situ hybridization (FISH) or fluorescently labeled TALEs, CRISPR.... Each ortholog was fused to a different fluorescent protein to create three colors. The specificity of...authors estimate that a minimum of 150-200 fluorescent protein molecules is necessary for a detectable signal...Addgene. A protein-tagging system for signal amplification in gene expression and fluorescence imaging. ... fluorescent protein...week! One of the newest applications is a colorful system that uses fluorescently labeled Cas9 to label ...modifications and applications Ma et al. developed this technique using standard fluorescence microscopy, and...

... blog Fluorescent Proteins 101: Fluorescent Biosensors Resources on Addgene.org Fluorescent protein...short), where a fluorescent protein is inserted into one of the loop regions of any G-protein-coupled receptor...NIR calcium reporter Near-infrared (NIR) fluorescent proteins promise better performance for in vivo use...calcium indicator using small monomeric NIR fluorescent proteins (miRFP670nano and miRFP720) and the Ca2+...engineering of potassium binding proteins, they developed a family of fluorescent potassium indicators with ...requirements (Lu et al. 2022). By fusing two bright fluorescent proteins with different thermal quenching rates (...protein plasmids and resources Fluorescent protein guide: Biosensors Collection ...

...how protein turnover is regulated in neurons. Protein turnover is the combined effect of protein synthesis...visualize the localization of protein synthesis. Alternatively, fluorescent protein tags can be used to facilitate...labeled with fluorescent probes that enable visualization of the location of the protein-protein interaction...out other protein tagging strategies Additional Resources on Addgene.org Fluorescent protein plasmids...observed with fluorescence microscopy. This application of AHA labeling is known as fluorescence noncanonical...you visualize protein turnover within a neuron? For my PhD I studied a synaptic protein that is linked...control the abundance of proteins in cells. Homeostatic regulation of protein abundance is essential for...

...Experiments Beware of Fluorescent Protein Aggregation Brush up on Your Fluorescent Protein Basics Resources...Browse Empty Plasmids to Fluorescent Protein Fusions Check out Our Fluorescent Protein Guide ...targeting a fluorescent donor to your protein is to hardwire the molecule directly into the protein’s peptide...incorporation of fluorescent probes into protein: hexahistidine-tag-mediated fluorescent labeling with (...during protein translation (Ermolenko et al., 2007). All that is required is that the proteins/nucleic...synthesized target protein molecules contain the donor and, more importantly there is no fluorescence background...resulting from nonspecific donor labeling. Fluorescent proteins (FPs) are the gold standard for genetically-encoded...

... 101: Green Fluorescent Protein Resources on Addgene.org Browse our Fluorescent Protein Collection Find...multiple fluorescent protein sequences to develop diverse fluorescent reporters is a useful application of ... sensors. Given the expanding palette of fluorescent proteins and the availability of powerful imaging...been used to produce complex fluorescent reporter constructs. These fluorescent reporters can be used to ... modules used in this method include fluorescent proteins, promoters, enhancers, polyadenylation signals...constructs were created, each containing a unique fluorescent protein with minimal spectral overlap between them...Table 1). In each construct, 3 copies of the fluorescent protein were fused in-frame to a tethering partner...

...fluorophores to proteins, pioneers of this technique were able to directly detect protein/protein interactions...Day RN, Davidson MW. Fluorescent proteins for FRET microscopy: monitoring protein interactions in living... CFP-YFP was the first to be used to study protein-protein interactions and several other pairs have been... can be used to create your choice of fluorescent fusion protein with your gene of interest: Plasmid... filters chosen for the donor fluorescence and the acceptor fluorescence. Additionally, this method could... the fluorescence decay time of the donor. When FRET occurs between the pairs, donor fluorescence is quenched...Articles About FRET: Piston DW, Kremers GJ. Fluorescent protein FRET: the good, the bad and the ugly. Trends...

...The Brainbow-1.0 construct contains three fluorescent proteins: RFP (red), YFP (yellow) and M-CFP (membrane...Brainbow-1.1 scales up the system to four fluorescent proteins, with recombination events specified by ...the principles remain the same. The first fluorescent protein in the construct, OFP (orange), is expressed...tandem invertible segments, each with two fluorescent protein coding sequences in opposite orientations...once Cre is removed from the system, the fluorescent protein whose coding sequence is closest to the promoter...Although the fluorophores used are fluorescently distinct, their protein sequences are highly homologous,...above. First, they screened a variety of fluorescent proteins to find those with ideal characteristics...

...permuted green fluorescent protein (GFP), and nLightR uses a red fluorescent protein, mApple. Kagiampaki ...findings that red fluorescent proteins tend to be less sensitive than green fluorescent proteins. nLightR did...the Addgene blog Fluorescent proteins 101: Fluorescent Biosensors Which Fluorescent Microscopy Technique...Plasmids Find Optogenetics Plasmids Visit the Fluorescent Protein Guide Pages ..., J., & Li, Y. (2019). A Genetically Encoded Fluorescent Sensor for Rapid and Specific In Vivo Detection...S., & Steinegger, M. (2022). ColabFold: Making protein folding accessible to all. Nature Methods, 19(6...neurons expressing nLightG or nLightR before/after application of NE (10 μM) and corresponding pixel-wise ΔF...

...tagging of every protein in the C. elegans genome. Although they tested fluorescent protein fusions, this...elegans even easier - now, one can generate a fluorescent protein fusion, transcriptional reporter, and loss-of-function...cassette (SEC). To make it possible fluorescently label proteins in C. elegans, they inserted the SEC...SEC into a synthetic intron between a fluorescent protein (FP) and a 3xFLAG tag (see construct schematic...located in this synthetic intron, and only the fluorescent protein and 3xFLAG-tag sequences are inserted into...repair template plasmid to create fluorescent and 3xFLAG-tag fusion proteins. The repair template and Cas9...Roller phenotype. These animals contain a fluorescent protein fusion for the targeted gene. After initial...

... Cre-lox Resource Guide Fluorescent Protein Resource Guide Fluorescent Protein Blog Posts ...Other proteins may also be utliized as scaffolds, including the set of red fluorescent proteins. The T-DOGGs...scaffold system is likely applicable to many types of proteins. For proteins with a modular structure,... similarly to GFP, but commonly used red fluorescent proteins dsRed, mCherry, and TdTomato do not induce...and derivatives GFP and YFP, but not red fluorescent proteins, as seen with T-DDOGs. Like T-DDOGs, Cre-DOG...., et al. “A nanobody-based system using fluorescent proteins as scaffolds for cell-specific gene manipulation...Caussinus, E., Kanca, O., and Affolter, M. “Fluorescent fusion protein knockout mediated by anti-GFP nanobody...

...Plasmid Collections Fluorescent Proteins Subcellular Localization Fluorescent Proteins: Subcellular Localization...marker protein is fused to EGFP, you may want to fuse your protein to a red fluorescent protein like mCherry... different fluorescent protein markers? Check out the Michael Davidson Fluorescent Protein Collection ... Michael Davidson Fluorescent Protein Collection Addgene Blog: Fluorescent Proteins Content last reviewed...pattern, you should fuse your protein of interest with a fluorescent protein that has a different emission...overlap between your fusion protein and the marker fusion protein using fluorescence microscopy, you can assess...Find plasmids encoding fluorescent fusion proteins with known subcellular localization patterns for colocalization...

...epitopes fused to the protein of interest are recognized and thus fluorescently labeled by scFv antibodies...works by bringing together an anchor protein and activator protein to drive transcription. The anchor consists...off all mRNA binding proteins as it travels along the mRNA to produce the protein product. The system,...RNA Imaging by Coat protein Knock-off, is made up of three components: a GFP protein that binds to the ...of a reporter mRNA via a PP7 coat protein (NLS-PCP-GFP) a RFP protein that binds to the 3’ UTR of the reporter... sites for the fluorescent proteins and an inducible promoter (Plasmid 64543or 64542) When the reporter...and the RFP proteins in the nucleus. During the first round of translation, the GFP proteins that are bound...

...cyan-excitable red fluorescent protein (mCyRFP1). Derived from cyan-excitable organge fluorescent protein (CyOFP1...on This New AAV Tool    mCyRFP1: Novel fluorescent protein that enables imaging of two biosensors simultaneously...as a donor in FRET (fluorescence resonance energy transfer) and FLIM (Fluorescence Lifetime Imaging Microscopy...Podcast Segment New bright monomeric red fluorescent protein - mScarlet! The Dorus Gadella lab has recently...recently developed a synthetic red fluorescent protein variant they call mScarlet. Bindels et al used a...evolution of new RFP variants The toolbox of fluorescent proteins (FP) for cellular imaging is constantly ...larger strategy to engineer improved red fluorescent proteins (RFPs) and, while the authors note that ...

...indicators (GECIs) are fluorescent proteins linked to calcium binding proteins (e.g., calmodulin or troponin...methods Many calcium reporters use a single fluorescent protein (FP), requiring only a simple measurement..... This sensor switches from green fluorescence to a red fluorescent state only by simultaneous (1) user-controlled...Structure-and mechanism-guided design of single fluorescent protein-based biosensors. Nature chemical biology...Resources on Addgene.org Addgene’s AAV Guide Fluorescent Proteins: Biosensors Addgene’s Viral Services  ...troponin C). Their fluorescent readouts are used to monitor calcium concentrations and transients in living... of the sensor, and (3) subsequent change in fluorescence only occurs at high calcium concentrations. ...

... Plasmid Collections Fluorescent Proteins Empty Backbones Fluorescent Proteins: Empty Backbones Blue/UV...Return to top Fluorescent Timers Fluorescent protein variants that change their fluorescence from the blue...Botman-Teusink Yeast Fluorescent Protein Collection Plasmid Kits Hamdoun Lab Fluorescent Protein Fusion Construct...Blog: Which Fluorescent Protein Should I Use? Addgene Blog: Choosing Your Fluorescent Proteins for Multi-Color...popular fluorescent proteins and empty backbones for expression and cloning of fusion proteins, along ...plasmid backbones with different fluorescent tags to create fusion proteins with your gene of interest. Plasmid...experiment. Tables are organized by color of the fluorescent protein. The content for this page was originally...

...encoding Cre-dependent expression of the fluorescent protein EYFP from the CAG promoter Viviana Gradinaru...AAV genome that ubiquitously expresses the fluorescent protein eYFP from the CAG promoter Viviana Gradinaru...hSyn1-eYFP An AAV genome that expresses the fluorescent protein eYFP from the hSyn1 promoter Viviana Gradinaru...

...sensors consist of a VSD fused to a single fluorescent protein (FP). This class of GEVI was the earliest... since rhodopsin proteins were initially evolved for ion transport, not fluorescence, they are not nearly...Chemogenetics Viral Vectors 101: Optogenetics Fluorescent Proteins 101: Introduction to FRET ... of fluorescence all have room for improvement. These sensors still have many useful applications, though... elements: a voltage-sensing component and a fluorescent output. The GEVI family of gadgets includes Ace...Chemigenetic sensors are better classified by their fluorescent reporter and the mechanism of their voltage sensing...rhodopsin functions as both the voltage detector and fluorescent signaler. The pro of this system is the kinetic...

...fluorophore binding aptamers and MS2-binding fluorescent proteins were the state of the art in RNA imaging...always on the lookout for better, brighter fluorescent proteins (FP) with improved tissue penetrance and...controls the activity of a microtubule-associated protein to regulate cell growth dynamics Article contributed...normally sits on microtubules to attract other proteins involved in their growth and extension. Now, they... domains. When both halves of the modified EB1 protein are overexpressed in cells, microtubule growth ...conformational changes, and as a consequence, EB1 protein function is inactivated by the separation of its...cellular dynamics. The photoinducible control of protein function is more specific and much faster than ...