...coding sequence for a green fluorescent protein (GFP). The success of the A-to-G conversion could be seen...through the success of the change in expression of GFP, for the stop codon should have changed from “TGA...cytometry is used to sort populations by mCherry and GFP expression to check for ABE’s. b) Quantification ... synthetic biology tool. These systems allow researchers to understand bacterial gene clusters that produce...

...plasmids Tagging human transcription factors with GFP Kevin White’s lab recently deposited a set of CRISPR...plasmids to tag human transcription factors with GFP. As part of the Encyclopedia of DNA Elements (ENCODE...series will help you find new CRISPR tools for your research.     Improved nucleic acid sequence detection...human genome, these donor plasmids will help researchers map transcription factor localization and binding...

...levels similar to that of a single AAV expressing GFP. The lab tested the base editors in vivo and achieved...voltage indicators, however, fall short compared to GFP-based sensors in terms of response and brightness...different labs and experimental systems in their own research. Cyanobacteria, a phylum of photosynthetic bacteria...

...considerably faster, with expression levels comparable to GFP during early embryogenesis (Figure 3). mScarlet-I3...through the middle of each embryo are shown, comparing GFP, wrmScarlet (mScarlet), and the new mScarlet3 and... focal adhesion marker paxillin (visualized with GFP). Reprinted with permission from Zhong et al, 2024...shorter than many popular fluorescence markers like GFP (510 nm), allowing co-imaging. And because bioluminescent...pathways and spontaneous mutations.  Here, the researchers were able to target multiple genes in M. abscessus...catalyze the luminescence reaction (Figure 5). The researchers used the reporter to visualize biologically relevant...

... from the default GFP (green) to RFP, CFP, or YFP fluorescence. In Brainbow 2.1, GFP is tagged with a ...and activate fluorescence of the downstream OFP, GFP, or RFP. Cells that express the construct but do ...generates cells that randomly express either nuclear GFP, membrane CFP, cytoplasmic YFP, or cytoplasmic RFP...exist for a variety of species, cell types, and research applications. Labeling neurons with Brainbow The...incorporates the highly photostable fluorophores mOrange2, EGFP, and mKate2 (red) to solve the problematically low... used alongside common, existing tools for fly research that use Flp recombination. In addition to the...small population size of CFP++ cells permitted researchers to longitudinally map the turnover of Langerhans...

... PS: they’re free. 3. Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection by ...Joachim Goedhart shares some strategies for generating GFP fusion proteins. From considering protein size and...10 blog posts from the year. 10. Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage...plasmids every quarter. The “hot plasmids” from last March were quite popular and represent the diversity of...academic job interview including defining your research vision, organizing your vision into a chalk talk...many model organisms people are using for their research. In this blog post, we’ve covered five model organisms...yeast, zebrafish, and worm. 4. Early Career Researcher Toolbox: Free Tools for Making Scientific Graphics...

...section. The GFP story has its own Franklin in Doug Prasher, who originally isolated the GFP gene but was...the identification of Green Fluorescent Protein (GFP) and its development into an essential tool in biology...they research, how they conduct their research, and in some cases, if they conduct their research. Even...inherent brokenness of scientific publishing and research funding. I’ve been aware of these problems since...

...vectors (eg GFP, Flag, YFP, Strep, TEV-V5) with either blasticidin or puromycin selection. Researchers at the...Since the discovery of GFP over 50 years ago, the growing spectrum of fluorescent proteins (FPs) has ...repair pathway, a new user-defined DNA sequence (like GFP) can be inserted at the DSB. CRISPR has allowed scientists...NanoLuc), fluorescent proteins (TagGFP2, TagBFP, TagRFP, and T2A-TurboGFP-PEST), and small epitope tags ...library containing several types of fluorescent (EGFP, tagRFP, mCherry) and nonfluorescent (Halo, SNAP...

...viral prep pAAV-CAG-GFP AAV9-X1.1 Controls Edward Boyden New serotype pAAV-hSyn-EGFP AAV PHP.eB Controls...AAV9. You can now target these cells with AAV-CAG-GFP packaged in AAV9-X1.1!  We also have eight new AAV-PHP.eB...enhancers that enable a whole host of (awesome) research not easily carried out before. We have a bunch...Controls Jonathan Ting New viral prep pAAV-CaMKIIa-EGFP AAV1 Controls Bryan Roth New serotype pAAV-Ef1a-DIO...

...substitutions to LanYFP and the addition of the enhanced GFP (EGFP)-type termini. mNeonGreen was reported as the ... to 3 times brighter than the most commonly used GFPs and YFPs. Its excitation maxima is at 506 nm and...partnership with Allele Biotechnology which allows researchers to deposit plasmids containing the fluorescent...rather than cyan and named the protein mTFP1. The researchers recommend using mTFP1 with a 445/30 nm excitation...protein. In vitro, mWasabi is 1.6-fold brighter than EGFP and is photostable. It also has narrow excitation...it does not significantly excite at 400 nm while EGFP does. Find mWasabi plasmids here! One of the most...function of the protein that they are fused to. The researchers constructed 22 different mTFP1 fusions to both...

...experiment, consider co-transfecting with GFP. This allows you to sort for GFP-positive cells and to enrich for...and their applications can be exhausting. A quick search for "CRISPR", short-hand for Clustered Regularly...such as: Sander JD & Joung JK, Nature Biotech, 2 March 2014. Or browse the articles related to the most...

...imaging with this set are mTagBFP2, EGFP or one of the improved GFP variants, mRuby2 or TagRFP-T, and an... / em 527 nm) can be imaged together but CFP and GFP (ex 488 nm / em 507 nm) show some crosstalk between...mammalian cells, one of the improved folding variants of EGFP like mEmerald or Clover is probably best; mNeonGreen...and have reported brightness measurements. Here, EGFP outperforms the improved folding variants, presumably... was a fellow at the Bauer Center for Genomics Research at Harvard University.       ...

...sequence. Using a variety of GFP and RFP fusions as their test genes, the researchers at the BIOFAB showed that...various combinations of protein tags (His, Avi, and GFP) as well as protease sites to facilitate protein ...terminators of varying strengths centered within GFP/RFP bicistronic reporters characterized in Cambray...Hopefully you know that, if you’re an academic researcher at a nonprofit institution, you can order plasmids...variety of model systems, thereby opening your research to a variety of experimental opportunities. The... predictable protein expression in bacteria, researchers at the BIOFAB and the Endy Lab created this kit... purification process by making it easy for a researcher to quickly generate N- and C-terminal epitope...

...regulation. To create iGluSnFR, the Looger lab wedged GFP between two halves of a bacterially derived periplasmic...increase in fluorescence intensity from the inserted GFP. After characterizing iGluSnFR in vitro, showing ...Loren Looger’s lab at HHMI Janelia Research Campus to give researchers the ability to monitor glutamate ...generation of iGluSnFR, the Looger lab swapped eGFP for sfGFP to create SF-iGluSnFR. This improved variant...iGluSnFR3 To improve upon the SF-iGluSnFR, the researchers did the following: Improve sensor’s photophysical.... In addition to the original PDGFR TMD, the researchers introduce iGluSnFR3 in two additional anchoring...Stargazin including a terminal PDZ ligand. The researchers recommend using the SGZ for in-culture work only...

...binding scFv that’s fused to GFP. When the multiple copies of the scFv-GFP fusion bind the SunTag scaffold...scFv that’s fused to a fluorescent protein such as GFP. The HA frankenbody works just like an antibody-based...application for basic and translational research.  Basic research applications of scFvs: The SunTag system...Plasmid Collection Find antibodies for neuroscience research from the Trimmer lab Check out Addgene's latest...

...each recruits a different fluorescent protein (BFP, GFP, or RFP) to the genomic loci of interest. Pairs of...hairpin sequence (sgRNA-Sirius-8XPP7) to recruit GFP to the loci of interest. To determine how the sgRNA-Sirius...plasmids for visualization Find plasmids for your research Read our CRISPR guide ...

...AAV-FLEX-Arch-GFP CAG Arch GFP Cre dependent 1, 9 Boyden 28305 pAAV-FLEX-ArchT-tdTomato CAG ArchT tdTomato...soCoChR-GFP Syn CoChR (soma-targeted) GFP Constitutive 9 Boyden 107712 pAAV-hSynapsin-FLEX-soCoChR-GFP Syn...pAAV-hsyn-Jaws-KGC-GFP-ER2 Syn Jaws GFP Constitutive 5, 8, rg* Boyden 84445 pAAV-CAG-FLEX-rc [Jaws-KGC-GFP-ER2] CAG...dependent 5 Boyden 99039 pAAV-CamKII-ArchT-GFP (PV2527) CaMKII ArchT GFP Constitutive 1, 5, 9, rg* Boyden ...* Deisseroth 58880 pAAV-Syn-ChR2(H134R)-GFP Syn ChR2/H134R GFP Constitutive 8, rg* Boyden 100054 pAAV....Constitutive 9 Harvey 59170 pAAV-Syn-Chronos-GFP Syn Chronos GFP Constitutive 1, 5, rg* Boyden 59171 pAAV-...Chrimson, ChRmine, ChroME, ChETA, C1V1 (t/t) Inhibitory Arch and variants eNpHR and variants Jaws stGtACR iC+...

...anti-FLAG-FB-GFP binding to FLAG-tag H2B for nuclear localization. (c) Applications of anti-FLAG-FB-GFP labeling...screening technique, researchers identified a synthetic lethal interaction between MARCH5 and WSB2 genes using...This set of new anti-FLAG frankebodies includes mEGFP, mRuby2, iRFP713, SNAP-tag, and HaloTag fusions,...fusions, while the anti-HA frankenbodies include mEGFP, mCherry, and HaloTag. Figure 1: (a) Anti-FLAG...have primarily used Cas9 to knock out genes, researchers at the Genetic Pertubation Platform at the Broad...screens because of its lowered editing rate. This research aimed to increase the potential of Cas12a genetic...

...interest (analyte), and accumulation of ATP activated GFP expression (receptor), then the transducer could ...plate reader, or any other instrument which measured GFP levels and converted that signal into a readable ... of a biosensor Some of the biosensors used in research are very sophisticated, but at their core they...

... constitutive promoter upstream of GFP increased expression of GFP six-fold, but a stabilized promoter...Researchers express genes of interest from plasmids in order to study gene function or to engineer cells...