We narrowed to 114 results for: CHL

1. Plasmids 101: Antibiotic Resistance Genes

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   ...elongation step) Bacteriostatic 10 ug/mL Chloramphenicol   Binds 50S ribosomal subunit; inhibits peptidyl...

2. Hot Plasmids June 2018 - Reverse transcriptase, nanobody, and protein-DNA interaction tools

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   ...genomic locus: Caspex  Article contributed by Susanna Bachle  Listen to the Caspex podcast segment Steven Carr...

3. Plasmids 101: Origin of Replication

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   ...amplifying more copies with the addition of chloramphenicol – make sure your growth conditions aren't working...

4. Viral Vectors 101: Parts of the AAV Transfer Plasmid

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   ...Woodard K, Cepko C, Guerin KI, Rego MA, Ersing I, Bachle SM, Kamens J, Fan M (2019) Adeno-Associated Virus...

5. Hot Plasmids - June 2020 - Barcoded CRISPR Library, Sparse Cell Labeling, Calcineurin Reporter, and DNA Staining Dye Alternative

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   ...sgRNA iBAR library Article contributed by Susanna Bachle Figure 1: Initial steps in the CRISPR-pooled...

6. A New Optogenetic Tool Based on AraC Controls Gene Expression with Blue Light

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   ...References and Resources References Romano E, Baumschlager A, Akmeriç EB, Palanisamy N, Houmani M, Schmidt...

7. Hot Plasmids - September 2019 - CRISPR, Auxin Inducible Degradation, MerMAID Channelrhodopsins, and Genome Looping

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   ...optogenetic toolbox Article contributed by Susanna Bachle Anion-conducting channelrhodopsins (ChR) are ion...

8. Summer SciComm Series: A PhD in Science Communication

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   ...poring over my grandparents’ medical textbooks by torchlight under the covers. In high school, I went to all...

9. Plasmids 101: Gram Negative and Positive Bacteria

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   ...types. In water, the dye dissociates into CV+ and Chloride (Cl-) ions. When the negatively charged iodine...

10. Addgene-Seeding Labs plasmid grant round 3: microbial drug resistance and biofilms, antidiabetic plant components, visualizing brain injury - and a glowing robot fish

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    ...By Susanna Bachle and Ina Ersing Seeding Labs and Addgene collaborate to provide scientific resources...

11. R Bodies: Membrane-Rupturing Microscopic Tools

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    ...Publication at Addgene 4. Petroni, G., Spring, S., Schleifer, K.-H., Verni, F. & Rosati, G. Defensive extrusive...

12. Plasmids 101: Transformation, Transduction, Bacterial Conjugation, and Transfection

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    ...in the lab. This is commonly done using calcium chloride which permeabilizes the cell membrane so the bacteria...

13. Expanding the Targeting Scope and Editing Efficiency of Adenine Base Editors

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    ... Susanna Bachle...

14. The Many Reasons for Irreproducible Research- and a Vaccine to Eradicate It

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    ...is in citizen science and behavioral ecology of cichlid fishes. Find David online at https://orcid.org/...

15. Antibodies 101: ChIP

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    ... with a PCR purification kit or with a phenol-chloroform extraction.    Fig. 1: Schematic overview...

16. Hot Plasmids December 2018 - C. elegans RNAi, TurboID, Detecting Viral Outbreaks, and Protein Binding Specificity Tools

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    ...active LacZ tetramer.  This tetramer cleaves chlorophenol red-b-D-galactopyranoside on the assay paper...

17. Cancer, Inflammation and Immunity - Harnessing the Body’s Defenses to Fight Cancer

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    ... Susanna Bachle...

18. Multiplex Genome Editing with CRISPR-Cpf1

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    ...Abudayyeh, O., Slaymaker, I., Makarova, K., Essletzbichler, P.,  Zhang, F. (2015). Cpf1 Is a Single RNA-Guided...

19. Addgene's Tips for Plasmid Quality Control

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    ...expected full plasmid sequences (examples include Benchling, Serial Cloner, and Snapgene), and save these ...

20. Working with Nuclear Receptors

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    ...reporter gene has over the years evolved from chloramphenicol acetyltransferase to luciferase, the basic ...