Addgene-Seeding Labs Plasmid Grant: Accelerating Science Globally - 5 Scientists, 4 Countries & 3 Continents!
Type
Blog Post
... Susanna Bachle...
We narrowed to 114 results for: CHL
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Plasmids 101: Antibiotic Resistance Genes
TypeBlog Post...elongation step) Bacteriostatic 10 ug/mL Chloramphenicol Binds 50S ribosomal subunit; inhibits peptidyl... -
CRISPR Guide
TypeCollection...alternatives Abudayyeh, O. O., Gootenberg, J. S., Essletzbichler, P., Han, S., Joung, J., Belanto, J. J., Verdine...Gootenberg, J. S., Abudayyeh, O. O., Lee, J. W., Essletzbichler, P., Dy, A. J., Joung, J., Verdine, V., Donghia..., O. O., Slaymaker, I. M., Makarova, K. S., Essletzbichler, P., Volz, S. E., Joung, J., Van Der Oost, ... -
Plasmids 101: Origin of Replication
TypeBlog Post...amplifying more copies with the addition of chloramphenicol – make sure your growth conditions aren't working... -
A New Optogenetic Tool Based on AraC Controls Gene Expression with Blue Light
TypeBlog Post...References and Resources References Romano E, Baumschlager A, Akmeriç EB, Palanisamy N, Houmani M, Schmidt... -
Viral Vectors 101: Parts of the AAV Transfer Plasmid
TypeBlog Post...Woodard K, Cepko C, Guerin KI, Rego MA, Ersing I, Bachle SM, Kamens J, Fan M (2019) Adeno-Associated Virus... -
Hot Plasmids - June 2020 - Barcoded CRISPR Library, Sparse Cell Labeling, Calcineurin Reporter, and DNA Staining Dye Alternative
TypeBlog Post...sgRNA iBAR library Article contributed by Susanna Bachle Figure 1: Initial steps in the CRISPR-pooled... -
Hot Plasmids June 2018 - Reverse transcriptase, nanobody, and protein-DNA interaction tools
TypeBlog Post...genomic locus: Caspex Article contributed by Susanna Bachle Listen to the Caspex podcast segment Steven Carr... -
Hot Plasmids - September 2019 - CRISPR, Auxin Inducible Degradation, MerMAID Channelrhodopsins, and Genome Looping
TypeBlog Post...optogenetic toolbox Article contributed by Susanna Bachle Anion-conducting channelrhodopsins (ChR) are ion... -
Summer SciComm Series: A PhD in Science Communication
TypeBlog Post...poring over my grandparents’ medical textbooks by torchlight under the covers. In high school, I went to all... -
Plasmids 101: Gram Negative and Positive Bacteria
TypeBlog Post...types. In water, the dye dissociates into CV+ and Chloride (Cl-) ions. When the negatively charged iodine... -
Addgene-Seeding Labs plasmid grant round 3: microbial drug resistance and biofilms, antidiabetic plant components, visualizing brain injury - and a glowing robot fish
TypeBlog Post...By Susanna Bachle and Ina Ersing Seeding Labs and Addgene collaborate to provide scientific resources... -
R Bodies: Membrane-Rupturing Microscopic Tools
TypeBlog Post...Publication at Addgene 4. Petroni, G., Spring, S., Schleifer, K.-H., Verni, F. & Rosati, G. Defensive extrusive... -
The Many Reasons for Irreproducible Research- and a Vaccine to Eradicate It
TypeBlog Post...is in citizen science and behavioral ecology of cichlid fishes. Find David online at https://orcid.org/... -
Plasmids 101: Transformation, Transduction, Bacterial Conjugation, and Transfection
TypeBlog Post...in the lab. This is commonly done using calcium chloride which permeabilizes the cell membrane so the bacteria... -
Antibodies 101: ChIP
TypeBlog Post... with a PCR purification kit or with a phenol-chloroform extraction. Fig. 1: Schematic overview... -
Expanding the Targeting Scope and Editing Efficiency of Adenine Base Editors
TypeBlog Post... Susanna Bachle... -
Hot Plasmids December 2018 - C. elegans RNAi, TurboID, Detecting Viral Outbreaks, and Protein Binding Specificity Tools
TypeBlog Post...active LacZ tetramer. This tetramer cleaves chlorophenol red-b-D-galactopyranoside on the assay paper... -
Cancer, Inflammation and Immunity - Harnessing the Body’s Defenses to Fight Cancer
TypeBlog Post... Susanna Bachle... -
Multiplex Genome Editing with CRISPR-Cpf1
TypeBlog Post...Abudayyeh, O., Slaymaker, I., Makarova, K., Essletzbichler, P., Zhang, F. (2015). Cpf1 Is a Single RNA-Guided...