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We narrowed to 253 results for: CIL

Showing: 41 - 60 of 253 results

  1. Pathways Over Time Plasmids Engage Students in Functional Genomics Research

    Type
    Blog Post
    ...strains are ∆ura3 mutants and cannot grow without uracil supplementation (6). Expression of MET/Met genes... isolate transformed strains on media that lack uracil, but contain methionine. (This allows cells carrying...Call to Action, Washington, DC. 2. President's Council of Advisors on Science and Technology [PCAST] (...

  2. Tips from the Repository Trenches: Using Barcodes to Track Samples

    Type
    Blog Post
    ...Addgene’s repository. For long-term storage in our facilities, every sample is transferred to a 2D barcoded...Pir1. The majority of plasmids contain either an ampicillin or kanamycin resistance gene. Every step along...kept at Addgene and another is sent to a storage facility). The third tube is used to fulfill orders. Breaking...

  3. Viral Production at Addgene

    Type
    Blog Post
    ...performed by the facility that produced the viral vector lot. Contact us to determine which facility produced ...collaboration with viral vector manufacturing facilities, such as the University of Pennsylvania Vector...

  4. CRISPR 101: Targeting RNA with Cas13a (C2c2)

    Type
    Blog Post
    ...with the Cas13a molecule through a uracil-rich stem loop and facilitates target binding and cleavage through...system, Cas9 uses both a tracrRNA and crRNA to facilitate binding and cleavage of target DNA respectively...

  5. Harnessing Bacterial Toxins for Allelic Exchange

    Type
    Blog Post
    ... lab harnessed a group of bacterial toxins to facilitate efficient recombineering (Khetrapal et al., 2015...reduce transcriptional readthrough into the MCS to facilitate the cloning of toxic products. To allow manipulation...system (Lazarus et al., 2019). Additionally, to facilitate modification of strains where conjugation may...

  6. PiggyBac-ing Through the Genome Editing Field

    Type
    Blog Post
    ...and a transposase. The piggyBac™ transposase facilitates the integration of the transposon specifically...achieved using the piggyBac™ transposase, which facilitates direct integration of the donor-transposon (carrying...standard homology directed repair template to facilitate the selection of cells containing your desired...

  8. Immunology Research Plasmids and Resources

    Type
    Collection
    ...8 CKLFSF8, CKLFSF8-V2 CNTF ciliary neurotrophic factor HCNTF CNTFR ciliary neurotrophic factor receptor... IL1RRP2 IL1RN interleukin 1 receptor antagonist ICIL-1RA, IL-1ra3, IL1F3, IL1RA, IRAP, MGC10430 IL2 interleukin...IL1RRP2, IL36R IL1RN interleukin 1 receptor antagonist ICIL-1RA, IL-1ra3, IL1F3, IL1RA, IRAP, MGC10430 IL2 interleukin...

  9. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ...develop better strategies for combating cancers. To facilitate innovative cancer research, the Reference Reagents...express the integrated gene. These vectors contain uracil-excision based (USER) sites, flanked by ADH1 and...by the Church lab, termed “dCas9-VPR”, which facilitates robust activation of target genes (20-40x greater...compensating for histidine (HIS3), leucine (LEU2), uracil (URA3), methionine (MET17), and lysine (LYS2) deficiencies...

  10. Antibodies

    Type
    Collection
    ...distributes ready-to-use recombinant antibodies to help facilitate scientific research. We are building a collection...

  12. What's New in CRISPR - Spring 2019

    Type
    Blog Post
    ...editing The best studied Cas12b nuclease from Alicyclobacillus acidoterrestris (AacCas12b) exhibits optimal...cells. The Feng Zhang lab identified a Cas12b from Bacillus hisashii, BhCas12b that is functional at 37 °C...

  13. Save Time and Money by Making Your Own Competent Cells

    Type
    Blog Post
    ...yourself time if you're transforming a plasmid with ampicillin resistance.  After a plasmid is introduced to...transformed cells are being plated on agar with ampicillin selection.  Notes and tips  We’ve found that...

  14. Mammalian RNAi Tools

    Type
    Collection
    ...RISC includes argonaute protein 2 (Ago2), which facilitates cleavage of the target. RNAi using siRNA follows...

  15. SARS-CoV-2 Pseudotyped Virus

    Type
    Collection
    ...BSL-3) agent, SARS-CoV-2 requires specialized facilities for the study of the live virus. Alternatively...

  17. Easing the Protein Purification Process with pCri

    Type
    Blog Post
    ... Protein Tags. Beyond E. coli: purifying from Bacillus subtilis and Pichia pastoris pCri also includes...other than E. coli, specifically the bacterium Bacillus subtilis and the yeast Pichia pastoris. The improved...

  18. Networking and Inspiring Young Scientists at Freshers Fairs

    Type
    Blog Post
    ...Society was founded in 1944 with the goal of facilitating networking between chemistry students, especially...over 170 members in its first year. The society facilitates opportunities to learn about Synthetic Biology...

  20. Scientists Map the SARS-CoV-2-Human Interaction Network

    Type
    Blog Post
    ...Additionally, they have an IRES-Puro marker to facilitate stable cell clone selection. Expression is driven...QBI UCSF. They are particularly interested in facilitating collaborative science, and making it accessible...
Showing: 41 - 60 of 253 results