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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 253 results for: CIL

Showing: 41 - 60 of 253 results

  1. Plasmids 101: Screening Strategies Used in Plasmid Cloning

    Type
    Blog Post
    ...contains an ampicillin resistance marker in the backbone. A selection on agar containing ampicillin will yield...on this below). Plating the transformation on ampicillin plates containing X-gal allows you to identify...

  2. Fluorescent Proteins 101: Luciferases

    Type
    Blog Post
    ... small (19 kDa) luminous shrimp (Oplophorus gracilirostris) luciferase and engineered to be extra bright...Luciferin ATP No Luminous shrimp(Oplophorus gracilirostris) Natural 455 19 Coelenterazine None Yes NanoLuc... NanoLuc®(derived from Oplophorus gracilirostris) Engineered  462 19 Furimazine None Optional AkaLuc...

  3. Plasmids 101: Yeast Vectors

    Type
    Blog Post
    ...efficient mechanism of homologous recombination to facilitate simple gene replacement/mutation. Yeast expression...L-hisitidine no S. cerevisiae  no yes   URA3 pyrimidine (uracil) yes - Grow with 5-FOA. S. cerevisiae yes - This...are used in the growth media. ura4+ pyrimidine (uracil) yes - Grow with 5-FOA*. S. pombe  no no *FOA in...

  4. Pathways Over Time Plasmids Engage Students in Functional Genomics Research

    Type
    Blog Post
    ...strains are ∆ura3 mutants and cannot grow without uracil supplementation (6). Expression of MET/Met genes... isolate transformed strains on media that lack uracil, but contain methionine. (This allows cells carrying...Call to Action, Washington, DC. 2. President's Council of Advisors on Science and Technology [PCAST] (...

  5. Tips from the Repository Trenches: Using Barcodes to Track Samples

    Type
    Blog Post
    ...Addgene’s repository. For long-term storage in our facilities, every sample is transferred to a 2D barcoded...Pir1. The majority of plasmids contain either an ampicillin or kanamycin resistance gene. Every step along...kept at Addgene and another is sent to a storage facility). The third tube is used to fulfill orders. Breaking...

  6. Antibodies

    Type
    Collection
    ...distributes ready-to-use recombinant antibodies to help facilitate scientific research. We are building a collection...

  7. CRISPR 101: Targeting RNA with Cas13a (C2c2)

    Type
    Blog Post
    ...with the Cas13a molecule through a uracil-rich stem loop and facilitates target binding and cleavage through...system, Cas9 uses both a tracrRNA and crRNA to facilitate binding and cleavage of target DNA respectively...

  8. Viral Production at Addgene

    Type
    Blog Post
    ...performed by the facility that produced the viral vector lot. Contact us to determine which facility produced ...collaboration with viral vector manufacturing facilities, such as the University of Pennsylvania Vector...

  9. Harnessing Bacterial Toxins for Allelic Exchange

    Type
    Blog Post
    ... lab harnessed a group of bacterial toxins to facilitate efficient recombineering (Khetrapal et al., 2015...reduce transcriptional readthrough into the MCS to facilitate the cloning of toxic products. To allow manipulation...system (Lazarus et al., 2019). Additionally, to facilitate modification of strains where conjugation may...

  10. PiggyBac-ing Through the Genome Editing Field

    Type
    Blog Post
    ...and a transposase. The piggyBac™ transposase facilitates the integration of the transposon specifically...achieved using the piggyBac™ transposase, which facilitates direct integration of the donor-transposon (carrying...standard homology directed repair template to facilitate the selection of cells containing your desired...

  11. Mammalian RNAi Tools

    Type
    Collection
    ...RISC includes argonaute protein 2 (Ago2), which facilitates cleavage of the target. RNAi using siRNA follows...

  12. SARS-CoV-2 Pseudotyped Virus

    Type
    Collection
    ...BSL-3) agent, SARS-CoV-2 requires specialized facilities for the study of the live virus. Alternatively...

  14. Antibody Plasmid Collection

    Type
    Collection
    ...amber codons in scFvs A new expression vector facilitating production and functional analysis of scFv antibody...

  15. Worm Expression Resources

    Type
    Collection
    ...Seydoux lab website . Co-CRISPR plasmids that facilitate detection of genome-editing events. Developed...

  16. CRISPR Plasmids - Bacteria

    Type
    Collection
    ...44251 pgRNA-bacteria BBa_J23119 SpeI + HindIII Ampicillin none, need Cas9 plasmid Qi Do you have suggestions...

  17. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ...develop better strategies for combating cancers. To facilitate innovative cancer research, the Reference Reagents...express the integrated gene. These vectors contain uracil-excision based (USER) sites, flanked by ADH1 and...by the Church lab, termed “dCas9-VPR”, which facilitates robust activation of target genes (20-40x greater...compensating for histidine (HIS3), leucine (LEU2), uracil (URA3), methionine (MET17), and lysine (LYS2) deficiencies...

  18. Arf GTPase Family

    Type
    Collection
    ...membrane traffic, they also play critical roles at cilia, centrosomes, the plasma membrane, lipid droplets...

  19. Save Time and Money by Making Your Own Competent Cells

    Type
    Blog Post
    ...yourself time if you're transforming a plasmid with ampicillin resistance.  After a plasmid is introduced to...transformed cells are being plated on agar with ampicillin selection.  Notes and tips  We’ve found that...

  20. What's New in CRISPR - Spring 2019

    Type
    Blog Post
    ...editing The best studied Cas12b nuclease from Alicyclobacillus acidoterrestris (AacCas12b) exhibits optimal...cells. The Feng Zhang lab identified a Cas12b from Bacillus hisashii, BhCas12b that is functional at 37 °C...
Showing: 41 - 60 of 253 results