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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 443 results for: DUR

Showing: 41 - 60 of 443 results

  1. A Look Back at One Year of Plasmid Sharing for COVID-19 Research

    Type
    Blog Post
    ...detect SARS-CoV-2 nucleic acids. Mammalian targets During the 2003 SARS outbreak, Hyeryun Choe’s lab identified...inhibitors of SARS-CoV-2 entry and become upregulated during viral infection.  Expression of SARS-CoV-2 viral...repository for many years while many were developed during the COVID-19 pandemic. Our mission to accelerate....434840 Tsang J, LaManna CM (2020) Open Sharing During COVID-19: CRISPR-Based Detection Tools. The CRISPR...Resources page Stay up-to-date on Addgene's operations during the pandemic Find SARS-CoV-2 pseudovirus plasmids...

  2. Simplify Cloning with in vivo Assembly

    Type
    Blog Post
    ... cloning procedures. Use high fidelity DNA polymerase This will minimize random errors during PCR: we ...bacterial recombination pathway to allow any cloning procedure to be performed using a simple two-step, 2 hr ...be immediately adopted by any laboratory. All procedures are performed using the same three step protocol... greater homology, so performing more complex procedures can be aided by extending homologous sequences...

  3. How Do I Place an Addgene Order Using a PO Number?

    Type
    Blog Post
    ...will be able to enter your PO number as payment during checkout. Placing your order online has some benefits...Add all your items to your cart. Click checkout. During the payment step of checkout, you will have the... invoice to the billing address that you listed during checkout. This invoice will reference your Addgene...Addgene order number and PO number that you entered during checkout. At this time, your organization will ...

  4. Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins

    Type
    Blog Post
    ...large scale cell mobility are essential processes during embryonic development. These are required for a...)? How do blood monocytes patrol blood vessels during immune responses (Auffray et al. 2007, Finsterbusch...Dynamics of neutrophil migration in lymph nodes during infection." Immunity 29.3 (2008): 487-496. PubMed...Jackson G., et al. "Macrophage and T cell dynamics during the development and disintegration of mycobacterial...Dynamic imaging of CD8+ T cells and dendritic cells during infection with Toxoplasma gondii." PLoS Pathog ...

  5. Multifunctional Neural Fibers: Modulation from Gut to Brain

    Type
    Blog Post
    ...how both the brain and visceral organs interact during various behaviors. Either tissue samples were harvested...physically hooked up to invasive, cumbersome systems during experiments – neither ideal for the intrepid researcher...affect their function (the gut twists and contorts during the digestion of food, while brain tissue is relatively...System for Wireless, Programmable Neuromodulation During Free Behavior. 2020 42nd Annual International Conference...

  6. Advice on Career Paths and the Green Card Process for International Researchers and Entrepreneurs

    Type
    Blog Post
    ...based green card applications and approved 91,023 during the first 3 quarters of 2017. As an international...have different roles, titles, and responsibilities during their careers - being aware of this can help take...Networking is extremely important for candidates during a job search and it is even more critical for international...for Neuroscientists to network with other members during the poster sessions, panels, exhibit floor, etc...and connect with people who could be beneficial during a career search. Doing these simple things will...

  7. Antibody Validation for Flow Cytometry

    Type
    Blog Post
    .... We find it useful to start with a screening procedure in easily genetically modified cell types (top..., D., McDowell, I., Southern, K., Reintsch, W., Durcan, T. M., Brown, C., Bandrowski, A., Virk, H., Edwards... C. (2023). Scaling of an antibody validation procedure enables quantification of antibody performance...., Benaliouad, F., Gileadi, O., McBride, H. M., Durcan, T. M., Edwards, A. M., Healy, L. M., Robertson...Implementation of an antibody characterization procedure and application to the major ALS/FTD disease gene...

  8. Revamp Your Lab Meetings With Creative Virtual Collaboration

    Type
    Blog Post
    ...Discussing data with colleagues can be more difficult during the pandemic era, with fewer opportunities for ...   Implement new tools for creative interaction During Zoom meetings, different tools can be used to promote...collaborative cloud tools such as Google Docs and Sheets during Zoom lab meetings to chip in ideas and take minutes...some degrees of belonging that was probably lost during these weird times of shift working and social distancing...

  9. Finding and Joining Your Dream Lab

    Type
    Blog Post
    ...other to be physically present at certain times during the day? We asked three lab heads how they go about...will provide a solid foundation on which to thrive during time spent as a graduate student or postdoc. Although...chosen research field is important, the end goal during this time is to be trained to think and execute...to ask lab heads, lab members and lab neighbors during the interview process. What if I want a non-academic...

  10. Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number

    Type
    Blog Post
    ... a look at how the stabilized promoters perform during these types of fluctuations. When cells are dividing... can also cause changes in plasmid copy number. During the shift from exponential to stationary phase,...promoters were able to better modulate gene expression during shifts in growth rate as compared to a constitutive...To simulate the increase in plasmid copy number during the transition to stationary phase, Segall-Shapiro...

  11. It’s Bioelectric! An Exciting Interdisciplinary Field of Research

    Type
    Blog Post
    ...communication is briefly inhibited in the entire worm during regeneration, the worm creates two heads. Subsequent...channel. Upon activation, it stays open for a longer duration. Hyperpolarizing chloride channel SwiChr++ - A...low-intensity blue light pulse and stays open for a longer duration upon activation. p-mcherry-C1-iC++_CA - A slow...junction protein-mediated cues control polarity during planarian regeneration." Developmental biology ...

  12. Transferable Skills Guide: Cross-team Communication

    Type
    Blog Post
    ..., too. And remember, if you discover during grad school or during your postdoc that you enjoy and have...know what kind of plate they were talking about during the meetings. And “plate” certainly wasn’t the ...experimental protocols. Clarify: Asking for clarification during a meeting can help get everyone on the same page...

  13. Viral Vectors 101: Virus Safety

    Type
    Blog Post
    ...protection! When centrifuging viruses (a necessary step during production), there is a significant risk for aerosol... via needlestick or cut. Following all sharps procedures for your organization will significantly reduce...best to be well-rested and alert the day of the procedure. Mice move much more quickly than cell cultures...understandably induce some nerves. Following safety procedures and practicing safety culture, however, can let...

  14. Polymerase Chain Reaction Overview and Applications

    Type
    Blog Post
    ...temperature. Taq was first identified in the late 1960s during research at hot springs in Yellowstone National...programmed thermocycler. For more details on this procedure check out our PCR protocol page, protocol video... common for dNTPs and other reagents to run low during the final cycles which would slow or stop PCR amplification...this fluorescent dye, the concentration of the DNA during the PCR reaction cycles is continuously detected...

  15. Antibodies 101: Conventional vs Spectral Flow Cytometry

    Type
    Blog Post
    ...special considerations. Conventional flow cytometry During conventional flow cytometry, light emitted by a...distinguish these two fluorophores from one another during spectral unmixing.     Figure 2: The ...telling exactly how many Cy7 molecules are added during that particular batch. This can present particular...upon antibody binding, leading to discrepancies during unmixing. Additionally, it is critical to include...

  16. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    ... It may be helpful to incorporate regular feeds during this incubation time to maintain cell viability...consider increasing the number of washes performed during your harvest. Validation Recombinant antibodies...temperature shifts. Of course, we are also cautious during the aliquoting and storage process to prevent dropped...PMC7665837. L'Abbé D, Bisson L, Gervais C, Grazzini E, Durocher Y. Transient Gene Expression in Suspension HEK293...

  17. Plasmids 101: Choosing an Antibiotic Resistance Gene

    Type
    Blog Post
    ...plasmids, or express a gene contained in a plasmid. During the propagation and/or transformation process, ...never receive a copy of the plasmid or will lose it during division. These non-plasmid-containing bacteria...while amp only affects the cell wall synthesis step during cell division. This is nifty because the recovery...it will induce mutations in the host/plasmid DNA during selection if it’s not completely bound up. While...

  18. Plasmids 101: Codon usage bias

    Type
    Blog Post
    ...generally segregate with the cell pellet during purification procedures. Even if you produce a large amount...for the entire translation process. For example, during high growth rate conditions, E. coli preferentially...biosynthetic enzymes may be preferentially charged during amino acid starvation thus resulting in higher ...

  19. Transferable Skills Guide: External Collaborations

    Type
    Blog Post
    ...time I’ve had a role in organizing collaborations. During my graduate studies at Karolinska Institutet I ...what was expected, and keeping track of speakers. During your graduate or postdoctoral work there are loads.... I am glad that I was able to build experience during my graduate studies in regard to reaching out to...Transferable Skills Guide Developing Transferable Skills During Science Training Resources on Addgene.org Check...

  20. Lab to Office Culture Shock

    Type
    Blog Post
    ...who referred to her lab as her family three times during a 10 minute conversation. Leaving a tight-knit ... “I’m a scientist.”    What should you expect during the lab → office transition? I’ve already provided...science,” and virtually every person you talked to during the day was also a scientist. But now you also ...upsetting, there are ways to get back to the bench during your off hours. DIY bio labs are cropping up in...
Showing: 41 - 60 of 443 results