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We narrowed to 46 results for: Dre recombinase

Showing: 41 - 46 of 46 results

  1. Delivery Methods for Generating iPSCs

    Type
    Blog Post
    ...lentiviral vector, but transient expression of Cre recombinase allows for deletion of the inserted transgenes...mice. Cre-deletable or Tet-inducible lentiviruses address some of these concerns, but overall integrating...

  2. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ...species. Indeed, in order to use MAGE, the λ Red recombinase enzymes need to be expressed and the native methyl-directed...plasmids (dubbed pORTMAGE) expresses the λ Red recombinase enzymes, as well as a dominant-negative mutator...system needs to be repressed in the host strain. To address this problem, Csaba Pál’s lab has created a set...

  3. Using AAV for Neuronal Tracing

    Type
    Blog Post
    ...conditional mutant mouse brain that expressed Cre recombinase only in pyramidal cells of the hippocampal region...viral proteins VP1, VP2, and VP3. To date several hundred variants, or serotypes, have been found naturally... an anterograde tracer and injected at several hundred sites across the entire mouse brain. Traced neurons...PubMed Central PMCID: PMC2698947. Merkel, S.F., Andrews, A.M., Lutton, E.M., Mu, D., Hudry, E., Hyman, ...

  4. CRISPR Guide

    Type
    Guide
    ...Plasmids: CRISPR transposases CRISPR Recombinases and Integrases Recombinases, and their subfamily integrases...mechanisms, including transposons, integrases, and recombinases, with Cas enzymes. CRISPR Transposases Transposon... large scale edits. These proteins, like Cre recombinase or phage derived serine integrases, insert donor...lab , pairs prime editing with a large serine recombinase (LSR). A prime editor is used to install the ...evoBxb1 or eeBxb1 are evolved versions of the recombinase with improved integration efficiencies in mammalian...& Hsu, P. D. (2022). Systematic discovery of recombinases for efficient integration of large DNA sequences...locus. Chromosome painting requires delivery of hundreds of gRNAs with target sites throughout the chromosome...

  5. Molecular Biology Reference

    Type
    Guide
    ... Guide . CRISPR Plasmids , Cre-lox and Other Recombinases , TALEN Plasmids and Kits , Genome Engineering...would be necessary to isolate a sequence of a few hundred or a few thousand base pairs from the entire human...puzzle. A scientist would need to run potentially hundreds of Sanger sequencing reactions on different pieces...limited to shorter reads of DNA, usually a couple hundred base pairs at most. Long-read sequencing technologies...

  6. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    ...of sequence-specific enzymes (e.g. nucleases, recombinases) which irreversibly excise or invert a specific...for detection of pre-mRNA splicing   The Gideon Dreyfuss lab has developed rapid-response luciferase (firefly...
Showing: 41 - 46 of 46 results