We narrowed to 107 results for: EGF
-
TypeBlog Post...for miRFPnanos, 35 kDa for miRFPs, and 27 kDa for EGFP) and a possibility to be used as internal tags due... normalization to fluorescence of co-transfected EGFP. e Originally reported as a monomer16, IFP2.0 was...
-
Multiple Plasmids at a Low Price: Kits from Addgene
TypeBlog Post...et al 2015). He has also constructed a library of eGFP-tagged human ORF clones to allow testing and comparison...be conveniently performed through the N-terminal eGFP tag carried on all constructs. The Cell-Free Protein... -
Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons
TypeBlog Post... and sequence overlap (coral mOrange2, jellyfish EGFP, and sea anemone mKate2.) They then successfully... is retained in Brainbow-3.0, but with mOrange2, EGFP and mKate2 as the fluorophores; mOrange2 is the ... -
Seeing Red: Simple GFP Photoconversion
TypeBlog Post...process worked well with the common GFP variants EGFP, mGFP4-T, and S65T GFP, and no red color was observed... -
The Michael Davidson Collection: One-Stop Shop for Fluorescent Proteins
TypeBlog Post...sun, from the standard oldies but goodies (e.g., EGFP and YFP) to the new and improved fruit colors (e.g... -
Choosing Your Perfect Empty Backbone
TypeBlog Post... allow you to visualize YGOI in vivo (try pcDNA3-EGFP). If you can get away with generating transiently... -
Hot Plasmids - June 2020 - Barcoded CRISPR Library, Sparse Cell Labeling, Calcineurin Reporter, and DNA Staining Dye Alternative
TypeBlog Post... They created plasmids for purification of tTALE-eGFP and tTALE-mCherry. In comparison to intercalating... -
New Neuroscience Tool: The iGluSnFR3 Glutamate Sensor
TypeBlog Post...second generation of iGluSnFR, the Looger lab swapped eGFP for sfGFP to create SF-iGluSnFR. This improved variant... -
FPbase: A new community-editable fluorescent protein database
TypeBlog Post...relating to that specific protein (for instance: EGFP). Users can “favorite” certain proteins, or create... -
A Practical Approach to Choosing the B(right)est Fluorescent Protein
TypeBlog Post... QY (EC*QY, sometimes normalized to the value of EGFP). The higher the number, the higher the theoretical... -
Multiplex Genome Editing with CRISPR-Cpf1
TypeBlog Post...lentiviral transduction One vector expresses Cas9, eGFP, and up to 4 gRNAs Each gRNA requires its own promoter... -
Plasmids 101: Knockout/Knock-In Plasmids
TypeBlog Post...protein with GFP, as seen in blue flame plasmid OCT4-eGFP-PGK-Puro from the Jaenisch lab. Figure... -
Multiplexed Capture of Promoter-enhancer 3D Chromatin Structures Using CRISPR
TypeBlog Post...lentiviruses delivering 1) a BioTAP-tagged dCas9 and an eGFP tag, and 2) gRNAs Number of loci targeted One ... -
Fluorescent Tagging of Endogenous Genes with SapTrap
TypeBlog Post...library containing several types of fluorescent (EGFP, tagRFP, mCherry) and nonfluorescent (Halo, SNAP... -
Light Sheet Fluorescence Microscopy
TypeBlog Post... nerve afferents labelled with a AAV8 expressing eGFP under the human ubiquitin C promoter. Anatomical... -
Plasmids 101: Multicistronic Vectors
TypeBlog Post...Multicistronic Element Expression Type MSCV-IRES-EGFP IRES Retroviral pMSCV-pBabeMCS-IRES-RFP IRES Retroviral... -
Minigenomes - a Safe Way to Study Dangerous Viruses Like the Ebola Virus
TypeBlog Post...non-viral) reporter gene (e.g. firefly luciferase or eGFP) flanked by the EBOV-specific gene start and gene... -
Hot Biosensors 2022: Year-End Roundup
TypeBlog Post...RTKs of interest, they create sensors for FGFR1, EGFR and ErbB2. And orthogonal sensors with different... -
Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection
TypeBlog Post...monomers in mammalian cells include: mTurquoise2, mEGFP, mNeonGreen, mScarlet(-I), and mCherry. Keep the... -
Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior
TypeBlog Post...incorporates the highly photostable fluorophores mOrange2, EGFP, and mKate2 (red) to solve the problematically low...