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We narrowed to 629 results for: EMB

Showing: 41 - 60 of 629 results

  1. Antibody Guide

    Type
    Collection
    ...Transfer proteins from the gel to a membrane. Incubate the membrane with an unconjugated primary antibody...block the membrane to remove excess antibody and prevent unwanted binding. Incubate the membrane with a ...Wash and block the membrane. Activate the HRP with a substrate kit. Image the membrane using either X-ray...tears, saliva, mucus) where it protects mucosal membranes Dimer with a valency of 4 IgA1; IgA2 IgD Coexpressed...short peptide linkers. Diabodies can be used for assembling protein nanostructures. Figure 2: An antibody...recognizes your primary antibody source species. Remember that recombinant antibodies will also have a source...

  2. Plant Plasmids and Resources

    Type
    Collection
    ...e.g. maize and Arabidopsis) and vectors for easy assembly of one or more gRNA expression cassettes - Qi-Jun...intronized Cas9 series of vectors and DNA modules for assembly of CRISPR/Cas9 constructs - Sylvestre Marillonnet...fluorescent seed selection and multiplex CRISPR/Cas9 assembly for fast generation of multiple Arabidopsis mutants...creating plant expression vectors: A modular plasmid assembly kit for multigene expression, gene silencing, ...Parts II and Infrastructure Kit for hierarchical assembly of plant multigene constructs - Johannes Stuttmann...cloning chassis and set of functional parts for assembling plant transformation vectors - Daniel Voytas....

  3. Arf GTPase Family

    Type
    Collection
    ...regulators of membrane traffic, they also play critical roles at cilia, centrosomes, the plasma membrane, lipid... collection of ARF family plasmids. ARF family members include regulatory GTPases, GEFs, and GAPs. Plasmid...regulatory GTPases have long been characterized as members of the RAS superfamily, though now have been argued...activity. Figure 1: Locations of ARF superfamily members. Figure from Szutl, et al., 2019. Browse ARF GTPase...2016). Tracing the archaeal origins of Eukaryotic Membrane-Trafficking System building blocks. Molecular ...

  4. Adenovirus Plasmids

    Type
    Collection
    ...Adenoviral A block for the AdenoBuilder genome assembly system. Block 6 with deletion in E3 and insertion...Adenoviral A block for the AdenoBuilder genome assembly system; consolidates the regions present in pAd5...Adenoviral A block for the AdenoBuilder genome assembly system. Block 1 with deletion in E1 and insertion...adenovirus serotype 5 (HAdV5) genome that can be assembled in a one-tube reaction. ID Kit Description PI ... Plasmids contain modular inserts for seamless assembly of multiple mutations, alterations, or transgene...

  5. Microbiology Resources

    Type
    Collection
    ...deposited tools and resources, or read our guide on assembly standards. Browse Plasmids by Microbe System Plasmids...chromosomes - Endy and Shearwin Labs Ralstonia-GG-Kit : Assembly of Ralstonia solanacearum species complex (Rssc...lipolytica Golden Gate tool kit : Integrative vector assembly of up to three transcription units in the oleaginous...circuit design in E. coli - Freemont Lab MoClo-YTK : Assembly of single and multi-gene constructs for S. cerevisiae...perform your synbio experiment. Synthetic Biology Assembly Standards Guide Bacterial Expression Systems :...

  6. COVID-19 Resources

    Type
    Collection
    ...envelope protein, E; nucleocapsid protein, N; and membrane protein, M), including plasmids for protein production...Anderson et al., 2020). BSG plasmids - (CD147), transmembrane glycoprotein of the immunoglobulin superfamily...proteolysis of SARS-CoV Spike protein, activating its membrane fusion function (Bosch et al., 2008). TACE plasmids...Llamas Required - Synthetic Nanobodies Against Membrane Proteins Return to top Pooled Libraries Pooled... in a new window) Content last reviewed on 26 September 2025. Do you have suggestions for other plasmids...

  7. Zinc Finger Consortium Reagents

    Type
    Collection
    ...method Modular Assembly Engineering Reagents - Engineer zinc finger arrays via Modular Assembly method Nuclease...finger proteins deposited by Zinc Finger Consortium members like the Joung Lab, Voytas Lab, and Wolfe Lab Plasmid... engineered zinc finger technology. Consortium members Keith Joung and Daniel Voytas have deposited at...

  8. Optogenetics Plasmid Collection

    Type
    Collection
    ...plasmids encode for microbial opsins, which are membrane channels and pores derived from algae and archaebacteria...ChR2) from Chlamydomonas reinhardtii . ChR2 is a membrane channel that opens in response to blue light and...properties as well as improve the expression and membrane targeting in host cells. Other microbial opsins...Promoter PI Return to top Content last reviewed: 25 September 2025 Do you have suggestions for other plasmids...

  9. Resolute Plasmid Collection

    Type
    Collection
    ...human genome with approximately 450 identified members. Many SLCs have mutations associated with disease... window) is a public-private partnership of 13 members from industry and academia financed by the Innovative...generate antibody/ high-affinity ligands. Data is embedded in an integrated knowledgebase that features, ...Superti-Furga CRISPR knockout library targeting human transmembrane transporters. Feedback Please send your feedback...

  10. Malate Dehydrogenase CUREs Community Collection

    Type
    Collection
    ...community. MCC members gain access to mentors, virtual and local professional development, membership to a local...research opportunities to their students and other members of the MCC community. They support educators interested... CUREs, and opportunities to mentor future MCC members. Find a regional hub (Link opens in a new window...

  11. p53 Pathway

    Type
    Collection
    ...DR5 Tumor necrosis factor receptor superfamily, member 10b E2F-1 E2F transcription factor 1 Fas Fas cell...protein 3 KAI CD82 molecule Maspin Serpin family B member 5 MDM2 MDM2 proto-oncogene, E3 ubiquitin protein...nexin, plasminogen activator inhibitor type 1), member 1 PERP TP53 apoptosis effector PIDD p53-induced...G2 arrest mediator candidate Scotin Shisa family member 5 Sestrins SESN1 SESN2 SESN3 Sestrins 1, 2, or ...

  12. Zebrafish Plasmid Collection

    Type
    Collection
    ...developmental biology. With its rapid development (embryogenesis is complete in ~3 days), short generation time...ease of care and breeding, and transparency of the embryo and larvae, it is easy to culture large populations...Project - Access the latest reference sequence assembly for the zebrafish genome. Zebrafish Model Organism...information about when genes are active in developing embryos. zfRegeneration - A database for gene expression...

  13. Ras Pathway

    Type
    Collection
    ...Resources Background Ras is a small GTPase and is a member of the G protein (guanine nucleotide binding protein...Ras signaling cascade that starts at the plasma membrane. Cancer Impact Ras is ubiquitously expressed in...RASSF8 RASSF9 RASSF10 Ras association domain family member RB1 Retinoblastoma 1 RCE1 Ras converting CAAX endopeptidase... in brain RHO RHOA RHOB RHOC Ras homolog family member ROCK ROCK1 ROCK2 Rho associated coiled-coil containing...

  14. Protocols for Molecular Biology, Plasmid Cloning, and Viral Preps

    Type
    Protocol
    ...on an agarose gel Watch the Video! DNA Ligation Assemble DNA using DNA ligase Bacterial Transformation ... new window) Link to Video Restriction Cloning Assemble plasmids by restriction digest and ligation Cloning...Add a short stretch of DNA to a plasmid Gibson Assembly Combine overlapping DNA fragments in a single ...

  15. Synthetic Biology - Overview

    Type
    Collection
    ...SynBio Resources SynBio Home Browse All SynBio Assembly Standards SynBio Blog Genome Engineering SynBio...repressors, activators, and more. Also contains pre-assembled genetic circuits such as logic gates and higher...System (Plant) MoClo Modular Cloning Modular PUF Assembly Kit Optogenetics Pertz Lab cpFRET Kit Rinehart...

  16. Antibody Plasmid Collection

    Type
    Collection
    ...plasmid-based antibodies. Description Article PI One-step assembly of antibody heavy- and light-chain DNAs into a...making synthetic nanobodies (sybodies) against membrane protein targets. Synthetic single domain antibodies...antibodies for the conformational trapping of membrane proteins. Elife. 2018 May 24;7. and Generation of...

  17. Distribution to Industry

    Type
    Collection
    ...Type PI Description High Complexity Golden Gate Assembly Standards Kit Cloning Eric Cantor Kit for using...predict high accuracy junction sets for Golden Gate assembly. MoClo Toolkit Plant Expression Sylvestre Marillonnet...Marillonnet 95 plasmids for cloning and assembling standardized parts to create eukaryotic multigene constructs...

  18. AAV Production in HEK293 Cells

    Type
    Protocol
    ...drifted. Filter the solution through a 0.22 μm membrane. Aliquot 500–1000 μL into sterile tubes. Store... pH to ~8.5 Filter sterilize through a 0.22 μm membrane. Store at 4 °C. Considerations Before You Start...supernatant as follows: Filter through a 0.45 μm PES membrane. Add 40% PEG such that the final PEG concentration...

  19. CRISPR References and Information

    Type
    Collection
    ...collection of protocols, materials, and publications by members of the Division of Signaling and Functional Genomics...cells pCas9_GFP ; gRNA empty vector Protocol at StemBook O'Connor-Giles Fly: gRNA and ssODNs design and...pSpCas9(BB) (pX330) PDF, 330 KB Parrott NEBuilder Assembly of CRISPR vectors using ssDNA oligos p201G Cas9...

  20. Bacterial Expression Systems

    Type
    Collection
    ...periplasmic space between the inner and outer membranes. Targeting proteins to the periplasm reduces bacterial... in plants. The Zinc Finger Consortium Modular Assembly Kits (Addgene #1000000005, 1000000010) contain...Collection page to find plasmids and kits for efficient assembly of TALEN constructs. The Zinc Finger Consortium...
Showing: 41 - 60 of 629 results