...multiplexing vectors at Addgene! Multiplexing in plants Qi-Jun Chen Lab Golden Gate/Gibson Assembly Multiplexing...

...designs. The second database, CrisprGE (published 27 Jun 2015), is noteworthy for its broad scope, curated...

...hSyn Parkinson's Jun Ding 185715 pAAV-hSyn-GFP-IRES-hSNCA SNCA GFP hSyn Parkinson's Jun Ding 185716 pAAV-hSyn-GFP-IRES-mSNCA...Parkinson's Jun Ding 185717 pAAV-hSyn-GFP-IRES-hSNCA (A11P/V70P) SNCA GFP hSyn Parkinson's Jun Ding 185718...Parkinson's Jun Ding 185719 pAAV-hSyn-GFP-IRES-hSNCA (S129D) SNCA GFP hSyn Parkinson's Jun Ding 185720...-GFP-IRES-hSNCA (A30P) SNCA Flag CMV Parkinson's Jun Ding 186012 pCMV Flag-LRRK2 K17A/K18A LRRK2 Flag ...

...MGC142142, SHIP, SHIP1, SIP-145, hp51CN JUN jun oncogene AP-1, AP1, c-Jun KRAS v-Ki-ras2 Kirsten rat sarcoma...LYK, MGC126257, MGC126258, PSCTK2 JUN jun oncogene AP-1, AP1, c-Jun KRAS v-Ki-ras2 Kirsten rat sarcoma...

...bfloGFPa1 and bfloGFPc1. Bomati et al., Sci Rep 2014 Jun 27;4:5469. MitoTimer plasmids to report mitochondrial...

...support reproducibility in science. Last Update: June 14, 2023 Workflow Timeline Day 1: Run SDS-PAGE and...

...available from Addgene are recommended for use in conjunction with the pLKO.1 TRC-cloning vector. Addgene Plasmid...

...effects of the injunction nationwide and, at exactly one month following the injunction, 59% of all postdocs...salary increase prior to the injunction still won’t after the injunction (see below). Of the institutions... known before the injunction, are raising salaries, even in light of the injunction. In addition, PIs ...per year. This post discusses how a nationwide injunction against the FLSA is affecting universities' decisions...below). However, on November 22nd a preliminary injunction against the FLSA updates was granted nationwide...salaries. What are institutions doing after the injunction? We are particularly interested in whether institutions...institutions planning to raise salaries prior to the injunction are still honoring these plans. We are now collecting...

... four types of cell junctions: adherens junctions, gap junctions, tight junctions, and desmosomes. Browse...Protein Collection . Return to top Cell Junctions Cell junctions are responsible for forming connections...Adherens Junctions Beta-catenin EGFP Alpha Yap 67937 mouse E-cadherin GFP (423) Adherens Junctions E-cadherin...EGFP-N1 Gap Junctions Connexin 43 EGFP Robin Shaw 49861 pDEST-mCherry-GJA1-20k-N1 Gap Junctions Connexin ... Gap Junctions Connexin 43 / GJA1 sfGFP David Spray 54959 mApple-VE-Cadherin-N-10 Tight Junctions VE-Cadherin...20 Adherens Junctions Beta-Catenin mCherry Michael Davidson 30313 pEGFP ZO1 Tight Junctions Zonula Occludens...Protein Blog Posts Background General Cytoskeletal Junctions Secretory Pathway Background The subcellular localization...

...at the ligation junction. Thus, all four bases at either side of the ligation junction were represented...containing one or more mismatches near the ligation junction with high efficiency (11, 21). Ligases are thought...discrimination at the upstream side of the ligation junction (the base pair providing the 3 ́-OH terminus to...Watson-Crick and mismatched) around the ligation junction in 16 wells of a 96-well plate, allowing 6 conditions...

...KCNIP2 KCNQ1 Examples of some genes encoding gap junction proteins cx43 GJB1 GJB2 Bioelectric states can...channels and even between many cells (through gap junction proteins which connect the cytoplasm of two cells...side should grow a head and a tail, and when gap junction-mediated electrical communication is briefly inhibited..., Néstor J., et al. "Long-range neural and gap junction protein-mediated cues control polarity during ...

... can see that those in teaching roles (such as adjuncts) and graduate and undergraduate students are exempted...this have not been received. Future actions for junior scientists and for academia Changes are afoot for... that tenured professors/mid-career scientists/junior faculty will all be most/least likely to have to...Research. He is interested in advocating for and with junior scientists, to help them navigate the scientific...

...meningitidis (Nm or Nme) NNNNGATT Campylobacter jejuni (Cj) NNNNRYAC Streptococcus thermophilus ... amino acids in length, Cas9 from Campylobacter jejuni (CjCas9) is even smaller than SaCas9 and is also...Park SW, Kim D, Kim K, Cho H-Y, Song DW, Lee KJ, Jung MH, Kim S, Kim JH, Kim JH, Kim J-S (2017) In vivo...small Cas9 orthologue derived from Campylobacter jejuni. Nat Commun 8: . https://doi.org/10.1038/ncomms14500...

...TJP1-mEGFP AICSDP-23 mEGFP Tight junction protein ZO-1 Tight junctions 91565 AAVS1-mEGFP AICSDP-35 mEGFP...109120 GJA1-mEGFP AICSDP-43 mEGFP Connexin-43 Gap junctions 109121 HIST1H2BJ-mEGFP AICSDP-52 mEGFP Histone...CTNNB1-mEGFP AICSDP-47 mEGFP Beta-catenin Adherens junctions 107579 RAB5A-mEGFP AICSDP-39 mEGFP Ras-related...

... IIS restriction enzymes and careful choice of junction sequences guided by experimentally-derived DNA...overhang observations, accurate “high-fidelity” junction sets for both the 12- and 24-fragment versions...lacZ cassette were designed and synthesized. In conjunction with BsaI-HFv2 (NEB #R3733), re-engineered to...

... amino acids in length, Cas9 from Campylobacter jejuni (CjCas9) is the smallest Cas9 ortholog characterized...Park SW, Kim D, Kim K, Cho H-Y, Song DW, Lee KJ, Jung MH, Kim S, Kim JH, Kim JH, Kim J-S (2017) In vivo...small Cas9 orthologue derived from Campylobacter jejuni. Nat Commun 8: . https://doi.org/10.1038/ncomms14500...

...ultimately leading to the formation of double Holliday junctions (dHJs). dHJs are four-stranded branched structures...resolved via cleavage in one of two ways - each junction resolution could happen on the crossing strand...orange arrows). If resolved dissimilarly (e.g. one junction is resolved on the crossing strand and the other...

...PMID: 22767332.  3. Kim, Dohoon, Chun-Hyung Kim, Jung-Il Moon, Young-Gie Chung, Mi-Yoon Chang, Baek-Soo...Central PMCID: 4176696. 2. Yusa, Kosuke, Roland Rad, Junji Takeda, and Allan Bradley. "Generation of transgene-free...19337237. PubMed Central PMCID: 2677165. 3. Jia, Fangjun, Kitchener D. Wilson, Ning Sun, Deepak M. Gupta...

...results of federally-funded research. 2)      In June, the White House honored 13 “Champions of Change... in celebration of open science. 3)      Also in June, the G8 Science Ministers issued a statement after...

...followed suit. Two Addgenies, Susanna Bachle and Yijun Zhang, recently attended virtual conferences (TAGC...pic.twitter.com/4MysvpzwkP — Samuel Guay (@SamGuay_) June 18, 2020 Final thoughts on virtual conferences...