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We narrowed to 166 results for: LOB;

Showing: 41 - 60 of 166 results
  1. Hot Plasmids - May 2022

    Type
    Blog Post
    ... system has a much lower error rate. For a one kilobase insertion, the researchers reported an editing...AAV system. dCas9-SSAP will make the editing of kilobase-scaled sequences more efficient and less risky...
  2. Antibodies 101: Isotypes

    Type
    Blog Post
    ...Garland Science; 2001. Structural variation in immunoglobulin constant regions. https://www.ncbi.nlm.nih....Science; 2001. The distribution and functions of immunoglobulin isotypes. Available from: https://www.ncbi....
  3. Addgene: The First Twenty Years

    Type
    Blog Post
    ...of CRISPR constructs and information across the globe.  “Read the Addgene guide: https://www.addgene.org...plasmids, was shared with researchers across the globe. As the pandemic slowed, Addgene began to think ...
  4. Celebrating 15 Years of Scientific Sharing

    Type
    Blog Post
    ... quickly. Hiring is hard but as a 3 year Boston Globe and BBJ Best Place to Work with a positive, supportive...making a difference. We serve scientists around the globe and our own staff is starting to reflect this diversity...
  5. Antibodies 101: Choosing the Right Isotype

    Type
    Blog Post
    ...antibodies used for detection bind to non-target immunoglobulins naturally present in the tissue sample. There...antibody fragments to block the endogenous immunoglobulins and reduce the background staining but they...
  6. Twenty Years of Addgene Sharing: CRISPR

    Type
    Blog Post
    ...positive impact on science and scientists around the globe. Our first story focuses on a technology that has...CRISPR tools are developed and used all over the globe. As of 2023, we have CRISPR plasmids deposited from...
  7. New CRISPR Tools: Cas7-11 and PASTE

    Type
    Blog Post
    ...deletions and small insertions, leaving a several kilobase-sized gap in the gene editing field. The AbuGoot...now developed PASTE, which can “drag and drop” kilobases’ worth of DNA with CRISPR-driven precision into...
  8. Overcoming the AAV Size Limitation for CRISPR Delivery

    Type
    Blog Post
    ...AAV. At 984 amino acids in length, Cas9 from Campylobacter jejuni (CjCas9) is the smallest Cas9 ortholog...editing with a small Cas9 orthologue derived from Campylobacter jejuni. Nat Commun 8: . https://doi.org/10.1038...
  9. Fluorescent Protein Guide: Subcellular Localization

    Type
    Collection
    ...mGreenLantern Gregory Petsko 221898 mito-mStayGold_pTwistCMVBetaGlobWPRENeo Mitochondria Mitochondrial targeting...mGreenLantern Gregory Petsko 221899 mStayGold-aTubulin_pTwistCMVBetaGlobWPRENeo Microtubules alpha-Tubulin mStayGold...
  10. 15 Hot Plasmids from 2017

    Type
    Blog Post
    ...recently deposited the Tol2 Gateway-Compatible Toolobox, which will allow scientists to quickly and easily...altered stoichiometry impacted reprogramming and global gene expression patterns. Polycistronic cassettes...
  11. MAPK Plasmids

    Type
    Collection
    ...collaboration with numerous research labs from around the globe, Addgene has assembled a MAPK plasmid collection...
  12. CRISPR Plasmids - Cascade-Cas3

    Type
    Collection
    ...degradation. This degradation can continue for many kilobases in one or both directions from the target region...
Showing: 41 - 60 of 166 results