We narrowed to 165 results for: LOB;
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TypeBlog Post...Garland Science; 2001. Structural variation in immunoglobulin constant regions. https://www.ncbi.nlm.nih....Science; 2001. The distribution and functions of immunoglobulin isotypes. Available from: https://www.ncbi....
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Hot Plasmids - May 2022
TypeBlog Post... system has a much lower error rate. For a one kilobase insertion, the researchers reported an editing...AAV system. dCas9-SSAP will make the editing of kilobase-scaled sequences more efficient and less risky... -
Addgene: The First Twenty Years
TypeBlog Post...of CRISPR constructs and information across the globe. “Read the Addgene guide: https://www.addgene.org...plasmids, was shared with researchers across the globe. As the pandemic slowed, Addgene began to think ... -
Reproducibility for Everyone: Lessons from an Open Science Collaboration
TypeBlog Post...organize a workshop for community scientists at the Global Community Bio Summit at the MIT Media Lab. As a...Levelling Up: Reproducibility for Everyone Addgene: a global resource working to enhance scientific reproducibility... -
Celebrating 15 Years of Scientific Sharing
TypeBlog Post... quickly. Hiring is hard but as a 3 year Boston Globe and BBJ Best Place to Work with a positive, supportive...making a difference. We serve scientists around the globe and our own staff is starting to reflect this diversity... -
Antibodies 101: Choosing the Right Isotype
TypeBlog Post...antibodies used for detection bind to non-target immunoglobulins naturally present in the tissue sample. There...antibody fragments to block the endogenous immunoglobulins and reduce the background staining but they... -
CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems
TypeBlog Post...to replace genes in the difficult-to-engineer Sulfolobus islandicus rod-shaped virus 2 (Mayo-Muñoz et ...Anti-CRISPR-Based and CRISPR-Based Genome Editing of Sulfolobus islandicus Rod-Shaped Virus 2. Viruses 10:695... -
Twenty Years of Addgene Sharing: CRISPR
TypeBlog Post...positive impact on science and scientists around the globe. Our first story focuses on a technology that has...CRISPR tools are developed and used all over the globe. As of 2023, we have CRISPR plasmids deposited from... -
Multiplexed Capture of Promoter-enhancer 3D Chromatin Structures Using CRISPR
TypeBlog Post...interactions at a promoter in the well-characterized beta globin locus. The two methods had similar rates of specificity...interactions at five sites in the well-characterized beta-globin locus were analyzed with CAPTURE 2.0, with two ... -
CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics
TypeBlog Post...reality, cellular DNA is a complex three-dimensional globule of chromatin. Chromatin is a combination of coiled...secondary structures (loops) and tertiary structures (globules) to achieve exquisite compaction—on the order ... -
New CRISPR Tools: Cas7-11 and PASTE
TypeBlog Post...deletions and small insertions, leaving a several kilobase-sized gap in the gene editing field. The AbuGoot...now developed PASTE, which can “drag and drop” kilobases’ worth of DNA with CRISPR-driven precision into... -
Overcoming the AAV Size Limitation for CRISPR Delivery
TypeBlog Post...AAV. At 984 amino acids in length, Cas9 from Campylobacter jejuni (CjCas9) is the smallest Cas9 ortholog...editing with a small Cas9 orthologue derived from Campylobacter jejuni. Nat Commun 8: . https://doi.org/10.1038... -
With an Eye Towards the Future, We Look Back at the March for Science
TypeBlog Post...studies viruses including HIV and is interested in global health policy. He is also chair of the postdoctoral... climate change. All the scientists – including global warming skeptics – insisted that more data and ... -
Advice on Career Paths and the Green Card Process for International Researchers and Entrepreneurs
TypeBlog Post...services to people located in all 50 states and globally. Casseus Law performs in-depth vetting of a candidate...Letter Resources on Addgene.org Careers at Addgene Global Plasmid Distribution ... -
15 Hot Plasmids from 2017
TypeBlog Post...recently deposited the Tol2 Gateway-Compatible Toolobox, which will allow scientists to quickly and easily...altered stoichiometry impacted reprogramming and global gene expression patterns. Polycistronic cassettes... -
MAPK Plasmids
TypeCollection...collaboration with numerous research labs from around the globe, Addgene has assembled a MAPK plasmid collection... -
Fluorescent Protein Guide: In Vivo Imaging
TypeCollection...as signals below 650 nm are highly absorbed by hemoglobin and cause undesirable background signals. Near-infrared... -
CRISPR Plasmids - Cascade-Cas3
TypeCollection...degradation. This degradation can continue for many kilobases in one or both directions from the target region... -
Resolute Plasmid Collection
TypeCollection...the pace of research in the field of SLCs to the global benefit of basic academic research as well as applied... -
Nuclear Receptor Signaling Atlas (NURSA) Plasmid Guide: Nuclear Receptors
TypeCollection...collaboration with research labs from around the globe, Addgene has assembled a Nuclear Receptor plasmid...