...plasmids, such as VSV-G (Vesicular Stomatitis Virus glycoprotein), however, may be used across both systems.  ...Envelope VSV-G Vesicular stomatitis virus G glycoprotein; Broad tropism envelope protein; Pseudotyped...

...incubate the sample with your antibody.  Both polyclonal and monoclonal antibodies can work well in ChIP...Antibodies 101: Immunoprecipitation  Antibodies 101: Polyclonal Antibodies Antibodies 101: Introduction to Antibodies...

...pOpen-UDG UDG Uracil DNA glycosylase 165541 pOpen-Fpg Fpg Formamidopyrimidine DNA glycosylase 165550 pOpen-T4 ...pOpen-T4 PGD T4 PGD (T4 pyrimidine DNA glycosylase, aka T4 Endonuclease V) 165540 pOpen-T7EI T7 Endonuclease ...

...encodes a single envelope protein called rabies glycoprotein (G). G-protein-coated RABV infects cells via...AAV Vector for TVA Expression AAV for Rabies Glycoprotein (G) expression  Find Retrograde AAV ...

... Ingredient Concentration or % Glycine or Glycine-HCl 25–200 mM SDS 0.1%–1% (w/v) ...

...). mAbs and rAbs have higher homogeneity than polyclonal antibodies because of their immunoglobin component...circumvents the batch-to-batch variability found in polyclonal antibodies, creating a potentially unlimited ...

...or supplying additional carbon sources such as glycerol or glucose (in moderation) may also serve to increase...fresh colony Sub-culturing directly from a frozen glycerol stock or agar stab may lead to loss of the plasmid...

...constructs were then combined to create a single polycistronic 15 kb insert. The final construct was then introduced...degree of identity, so not suited to generate polycistronic containing multiple identical polyadenylation...

...allows for the following to occur: A) Multiple polyclonal secondary IgGs can bind to different epitopes... Reagent Pros Cons Antibodies Bivalent Polyclonal Can be labeled with multiple dyes Commercially...

...While monoclonal and polyclonal antibodies are readily available from several sources, fewer sources ...Antibodies Plasmid-based Recombinant Antibodies Polyclonal Antibodies Resources on addgene.org Addgene's...

...Expensive.   Kanamycin Kanamycin, or kan, is an aminoglycoside which inhibits protein synthesis. The kan resistance...The NPTII gene confers resistance to several aminoglycosides, including G418 which is commonly used for ...

...such as 0.1M glycine pH 2.5 and immediately neutralized with Tris pH 8-8.5. Low pH glycine is very effective...

...But be careful — antibiotic mechanisms vary! Aminoglycoside antibiotics also block protein synthesis, and...protects against any of them. But different aminoglycosides work on different host species: kanamycin targets...

...General expression mRNA Mammalian promoter from phosphoglycerate kinase gene.  Constitutive  Widespread expression...mRNA  Strong yeast expression promoter from glyceraldehyde 3-phosphage dehydrogenase Constitutive  Very...

...avidin, is found in the egg white. Avidin is a glycoprotein that can bind four molecules of biotin. ...purified avidin (Kresege et al., 2004). Avidin is a glycoprotein that consists of four identical subunits that...

... a natural coenzyme, that strongly binds the glycoprotein, avidin or a similar protein, streptavidin. ...various cell surface proteins that contain a monoglycine residue at the N terminus with a small peptide...

...terminators can be used to precisely tune your polycistronic E. coli expression constructs. Some applications...and Quantification of Ribosome Inhibition by Aminoglycoside Antibiotics in Living Bacteria Using an Orthogonal...

...frequently alter their DNA by adding methyl groups, glycosyl groups, and other modifications to their bases... the interactions between bacteriophage-encoded glycan binding proteins and carbohydrates." Current opinion...

... single vector. sgRNAs are expressed as polycistronic glycine tRNA-gRNA genes (PTGs) and then PTG cassettes...Gateway recombination-based cloning of complex polycistronic lentiviruses. Can express Cas9 with up to 3 ...

...EAAC1 Rat Mouse IgG2a 206573 Anti-Glycine receptor Alpha3 [N424/45R] Glycine receptor Alpha3 Human Mouse IgG2a... IgG2a 206574 Anti-Glycine receptor Alpha3L [N424/48R] Glycine receptor Alpha3L Human Mouse IgG2a 206575...receptor Mouse Mouse 206774 Glycine receptor Alpha3 scFv [N424/45] N424/45 scFv Glycine receptor Alpha3 Human...