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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 149 results for: LYC

Showing: 41 - 60 of 149 results

  1. Viral Vectors 101: Gamma-Retroviral Packaging Systems

    Type
    Blog Post
    ...plasmids, such as VSV-G (Vesicular Stomatitis Virus glycoprotein), however, may be used across both systems.  ...Envelope VSV-G Vesicular stomatitis virus G glycoprotein; Broad tropism envelope protein; Pseudotyped...

  2. Antibodies 101: ChIP

    Type
    Blog Post
    ...incubate the sample with your antibody.  Both polyclonal and monoclonal antibodies can work well in ChIP...Antibodies 101: Immunoprecipitation  Antibodies 101: Polyclonal Antibodies Antibodies 101: Introduction to Antibodies...

  3. Rabies and Neuronal Tracing

    Type
    Blog Post
    ...encodes a single envelope protein called rabies glycoprotein (G). G-protein-coated RABV infects cells via...AAV Vector for TVA Expression AAV for Rabies Glycoprotein (G) expression  Find Retrograde AAV ...

  5. Corralling and Cornering the Goldilocks Antibody

    Type
    Blog Post
    ...). mAbs and rAbs have higher homogeneity than polyclonal antibodies because of their immunoglobin component...circumvents the batch-to-batch variability found in polyclonal antibodies, creating a potentially unlimited ...

  6. Immunology Research Plasmids and Resources

    Type
    Collection
    ...MGC149868, hCS-L CSPG5 chondroitin sulfate proteoglycan 5 (neuroglycan C) MGC44034, NGC CTF1 cardiotrophin 1...MGC99678 GPHA2 glycoprotein hormone alpha 2 A2, GPA2, MGC126572, ZSIG51 GPHB5 glycoprotein hormone beta ...subunit ADTB3, ADTB3A, HPS, HPS2, PE AZGP1 alpha-2-glycoprotein 1, zinc-binding ZA2G, ZAG B2M beta-2-microglobulin...osteosarcoma viral oncogene homolog AP-1, C-FOS GSK3B glycogen synthase kinase 3 beta - HRAS v-Ha-ras Harvey ...laevis) DAND4, MGC119894, MGC119895, MGC96951 CGA glycoprotein hormones, alpha polypeptide CG-ALPHA, FSHA, ...MIF macrophage migration inhibitory factor (glycosylation-inhibiting factor) GIF, GLIF, MMIF MLN motilin...gfg-1 OGFR opioid growth factor receptor - OGN osteoglycin DKFZp586P2421, OG, OIF, SLRR3A OPRD1 opioid receptor...

  7. Plasmids 101: Optimizing Plasmid Yields

    Type
    Blog Post
    ...or supplying additional carbon sources such as glycerol or glucose (in moderation) may also serve to increase...fresh colony Sub-culturing directly from a frozen glycerol stock or agar stab may lead to loss of the plasmid...

  8. MXS Chaining

    Type
    Blog Post
    ...constructs were then combined to create a single polycistronic 15 kb insert. The final construct was then introduced...degree of identity, so not suited to generate polycistronic containing multiple identical polyadenylation...

  9. Plasmids 101: Secondary Nanobody Toolbox

    Type
    Blog Post
    ...allows for the following to occur: A) Multiple polyclonal secondary IgGs can bind to different epitopes... Reagent Pros Cons Antibodies Bivalent Polyclonal Can be labeled with multiple dyes Commercially...

  10. Antibodies 101: Producing Recombinant Antibodies

    Type
    Blog Post
    ...While monoclonal and polyclonal antibodies are readily available from several sources, fewer sources ...Antibodies Plasmid-based Recombinant Antibodies Polyclonal Antibodies Resources on addgene.org Addgene's...

  11. Plasmids 101: Choosing an Antibiotic Resistance Gene

    Type
    Blog Post
    ...Expensive.   Kanamycin Kanamycin, or kan, is an aminoglycoside which inhibits protein synthesis. The kan resistance...The NPTII gene confers resistance to several aminoglycosides, including G418 which is commonly used for ...

  13. Plasmids 101: Shuttle Vectors

    Type
    Blog Post
    ...But be careful — antibiotic mechanisms vary! Aminoglycoside antibiotics also block protein synthesis, and...protects against any of them. But different aminoglycosides work on different host species: kanamycin targets...

  14. Plasmids 101: The Promoter Region – Let's Go!

    Type
    Blog Post
    ...General expression mRNA Mammalian promoter from phosphoglycerate kinase gene.  Constitutive  Widespread expression...mRNA  Strong yeast expression promoter from glyceraldehyde 3-phosphage dehydrogenase Constitutive  Very...

  15. Open Enzyme Collection

    Type
    Collection
    ...pOpen-UDG UDG Uracil DNA glycosylase 165541 pOpen-Fpg Fpg Formamidopyrimidine DNA glycosylase 165550 pOpen-T4 ...pOpen-T4 PGD T4 PGD (T4 pyrimidine DNA glycosylase, aka T4 Endonuclease V) 165540 pOpen-T7EI T7 Endonuclease ...

  17. Plasmids 101: Biotinylation

    Type
    Blog Post
    ...avidin, is found in the egg white. Avidin is a glycoprotein that can bind four molecules of biotin. ...purified avidin (Kresege et al., 2004). Avidin is a glycoprotein that consists of four identical subunits that...

  18. Multiple Plasmids at a Low Price: Kits from Addgene

    Type
    Blog Post
    ...terminators can be used to precisely tune your polycistronic E. coli expression constructs. Some applications...and Quantification of Ribosome Inhibition by Aminoglycoside Antibiotics in Living Bacteria Using an Orthogonal...

  19. Technique: Probe Phage Genomes for Host Binding Proteins

    Type
    Blog Post
    ...frequently alter their DNA by adding methyl groups, glycosyl groups, and other modifications to their bases... the interactions between bacteriophage-encoded glycan binding proteins and carbohydrates." Current opinion...

  20. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ... single vector. sgRNAs are expressed as polycistronic glycine tRNA-gRNA genes (PTGs) and then PTG cassettes...Gateway recombination-based cloning of complex polycistronic lentiviruses. Can express Cas9 with up to 3 ...
Showing: 41 - 60 of 149 results