We narrowed to 144 results for: LYC

...single colony and do not subculture directly from glycerol stocks, agar stabs, or liquid cultures. Freshly...higher plasmid yield that colonies streaked from a glycerol stock. Incubation for 12-16 hours tends to give...

...one epitope. (It can sometimes be an issue with polyclonal antibodies, so it's good to keep epitope availability...Antibodies 101: Monoclonal Antibodies Antibodies 101: Polyclonal Antibodies Antibodies 101: Introduction to Antibodies...

...for antibody production have several drawbacks. Polyclonal antibodies produced in animals may be variable...potential for further engineering as compared to polyclonal or monoclonal antibodies. You can learn more ...

...plasmids, such as VSV-G (Vesicular Stomatitis Virus glycoprotein), however, may be used across both systems.  ...Envelope VSV-G Vesicular stomatitis virus G glycoprotein; Broad tropism envelope protein; Pseudotyped...

...Cassettes transfection gRNAs expressed from a polycistronic transcript  Requires co-transfection of Cas9...Cassettes transfection gRNAs expressed from a polycistronic transcript Requires co-transfection of Cas9  ...

...incubate the sample with your antibody.  Both polyclonal and monoclonal antibodies can work well in ChIP...Antibodies 101: Immunoprecipitation  Antibodies 101: Polyclonal Antibodies Antibodies 101: Introduction to Antibodies...

... Ingredient Concentration or % Glycine or Glycine-HCl 25–200 mM SDS 0.1%–1% (w/v) ...

...encodes a single envelope protein called rabies glycoprotein (G). G-protein-coated RABV infects cells via...AAV Vector for TVA Expression AAV for Rabies Glycoprotein (G) expression  Find Retrograde AAV ...

...). mAbs and rAbs have higher homogeneity than polyclonal antibodies because of their immunoglobin component...circumvents the batch-to-batch variability found in polyclonal antibodies, creating a potentially unlimited ...

...or supplying additional carbon sources such as glycerol or glucose (in moderation) may also serve to increase...fresh colony Sub-culturing directly from a frozen glycerol stock or agar stab may lead to loss of the plasmid...

...While monoclonal and polyclonal antibodies are readily available from several sources, fewer sources ...Antibodies Plasmid-based Recombinant Antibodies Polyclonal Antibodies Resources on addgene.org Addgene's...

...Expensive.   Kanamycin Kanamycin, or kan, is an aminoglycoside which inhibits protein synthesis. The kan resistance...The NPTII gene confers resistance to several aminoglycosides, including G418 which is commonly used for ...

...constructs were then combined to create a single polycistronic 15 kb insert. The final construct was then introduced...degree of identity, so not suited to generate polycistronic containing multiple identical polyadenylation...

...allows for the following to occur: A) Multiple polyclonal secondary IgGs can bind to different epitopes... Reagent Pros Cons Antibodies Bivalent Polyclonal Can be labeled with multiple dyes Commercially...

...But be careful — antibiotic mechanisms vary! Aminoglycoside antibiotics also block protein synthesis, and...protects against any of them. But different aminoglycosides work on different host species: kanamycin targets...

...such as 0.1M glycine pH 2.5 and immediately neutralized with Tris pH 8-8.5. Low pH glycine is very effective...

...General expression mRNA Mammalian promoter from phosphoglycerate kinase gene.  Constitutive  Widespread expression...mRNA  Strong yeast expression promoter from glyceraldehyde 3-phosphage dehydrogenase Constitutive  Very...

...avidin, is found in the egg white. Avidin is a glycoprotein that can bind four molecules of biotin. ...purified avidin (Kresege et al., 2004). Avidin is a glycoprotein that consists of four identical subunits that...

...terminators can be used to precisely tune your polycistronic E. coli expression constructs. Some applications...and Quantification of Ribosome Inhibition by Aminoglycoside Antibiotics in Living Bacteria Using an Orthogonal...

... a natural coenzyme, that strongly binds the glycoprotein, avidin or a similar protein, streptavidin. ...various cell surface proteins that contain a monoglycine residue at the N terminus with a small peptide...