...that only further impairs the patient. When I first met AJ and his family, he did not seem much different...

...will get the research funded,” she says. Baradaran met Addgene Executive Director Joanne Kamens several ...for Biomedical Research. Drug development was something Stegmeier has been interested in for a long time...

...need just from reading up on a topic, but I haven’t met any.” A few other tips: “Learn HTML, write a lot ...

... contribute more. Assuming basic needs are being met (i.e. enough pay and benefits to live on), the factors...Good relationships with clients and colleagues Sometimes this means you have to fire a good performer if...when making decisions, assignments and polices. Sometimes the boss has to be the boss and make difficult...deadlines (there’s that need for flexibility, sometimes taken to an extreme by scientists) Are highly ...

...it? how did you get there? Many of those people I met through AWIS (Association for Women in Science); ... and embedding individual diversity to get from gamete to a visibly complex - and unique - adult. My initial...

...Using the Indirect ELISA Method Antibody Validation Using the Indirect ELISA Method You may also like... ...of each well at 450 nm on the spectrophotometer. Use the spectrophotometer to generate a standard curve... we list the specific equipment, reagents, and methods that we use in our lab at Addgene. Equipment and...an automated ELISA plate washer. Equipment Spectrophotometer compatible with 96-well plates 1–10 µL single...PBS, 1X pH 7.4, VWR 45000-446 TMB (3,3',5,5'-tetramethyl-[1,1'-biphenyl]-4,4'-diamine) substrate kit, ...

...between neuroscience and other fields. For instance I met one researcher studying how the microbiome can affect...engineering E. coli metabolism, maybe you’ll be able to apply your knowledge of metabolism to better understand...there were also people ready to discuss their VR helmets, gait tracking technologies, and brain monitors...receptors to the brain, and probing how fatty acid metabolites affect brain function. These scientists pulled...

...speed while ensuring our rigorous QC standards are met, we are taking a unique hybrid approach compared ...Mediated by SARS Coronavirus Spike Proteins. In: Methods in Molecular Biology. Humana Press, pp 331–338 ...available at Addgene Read about SARS-CoV-2 detection methods Resources on Addgene.org Find all plasmids from...

...lysosomes. Though a number of biosensors existed that met some of the criteria necessary for studying endosomes...activate the proteasome. Clift et al. tested multiple methods for TRIM-Away delivery. For small cell populations... recent Nature Communications article “SRpHi ratiometric pH biosensors for super-resolution microscopy...be photostable over a broad pH range. The four biometric probes successfully targeted endosomes under a...modules to repress or activate gene expression, sometimes incorporating chemically inducible approaches ...

...Industry interviews, however, were more structured. I met with several individual and/or small groups, usually...have a theme too, which is something not inherently captured by the STAR method. I like to think of the ...stories to tell in your interview The STAR interview method is a popular way to answer behavioral interview...situation, task, action, and results. While this method seems simple, I found it hard to remember to describe...interview. Each interview is an opportunity to learn something and then apply it to the next interview. Perhaps...underdressed, but read emails from HR carefully because sometimes companies have relaxed dress codes for interviews...

...hint of CRISPR editing in human embryos has been met with a storm of media coverage. But the paper published...

...Symposium A year ago, trainees from around Boston met to discuss their visions for a successful and sustainable...

...Aims section highlights each milestone that must be met in order to deem your proposal successful. Typical..., if not all, of the limitations of current methodologies. This section sets the stage for why your funded...

...order for FRET to occur several constraints must be met. The donor and acceptor fluorophores must be compatible... determine whether two proteins are within 10 nanometers of each other, or measure the tension in the ...Nicolas. "FRET-based molecular tension microscopy." Methods 94 (2016): 33-42. PubMed PMID: 26210398. Additional...

...attend the annual NPA meeting to learn more. Having met many people at that conference, I later became involved...

.... I had no idea this was happening even though I met with X on a weekly basis! The silence and maybe even... opportunities. I still need to check myself sometimes to make sure I am delegating well, but it does...

...compliment the STEM curriculum. As it happened, I’d met some of the 8th grade science teachers socially and...minds, but I think many of the students learned something. Based on the initial guidelines I received from...

...all ages, learned a lot about the dinosaurs, and met wonderful people. When I started volunteering, I ...experience taught me something about myself and my future career. If it wasn’t something I liked – well, I...through developing and leading a budding nonprofit, something I had zero experience with before founding CINSO...

...above this level, and certain job duty tests must be met. In the Department of Labor’s general guide to the...

... collaborating with your neighboring lab, you've met interesting people at conferences and you have successfully...questions become popular. There are only so many methods and machines one team can learn so linking together...associations and specific projects where you share methods and equipment between lab groups and departments...- if you are aware of a problem or a need for something, usually someone else has realized this too. Begin... about your ideas and don’t be afraid to make something concrete happen. You may have more willing collaborators...