We narrowed to 848 results for: Met
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TypeBlog Post...to work! Set up similarly to the online class, we met as a group for sessions covering individual portions... new technique, then type up the materials and methods. Over time that portion of the paper will be generated...
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Using Addgene's New Viral Service for Your Research
TypeBlog Post...booming, but, over the years, many researchers we’ve met with have asked if we can help them share and distribute... -
Running for Rare Disease, Running for FOP, Running for AJ
TypeBlog Post...that only further impairs the patient. When I first met AJ and his family, he did not seem much different... -
Management for Scientists: What Makes a Good Manager Anyway?
TypeBlog Post... contribute more. Assuming basic needs are being met (i.e. enough pay and benefits to live on), the factors...Good relationships with clients and colleagues Sometimes this means you have to fire a good performer if...when making decisions, assignments and polices. Sometimes the boss has to be the boss and make difficult...deadlines (there’s that need for flexibility, sometimes taken to an extreme by scientists) Are highly ... -
Antibody Validation Using the Indirect ELISA Method
TypeProtocol...Using the Indirect ELISA Method Antibody Validation Using the Indirect ELISA Method You may also like... ...of each well at 450 nm on the spectrophotometer. Use the spectrophotometer to generate a standard curve... we list the specific equipment, reagents, and methods that we use in our lab at Addgene. Equipment and...an automated ELISA plate washer. Equipment Spectrophotometer compatible with 96-well plates 1–10 µL single...PBS, 1X pH 7.4, VWR 45000-446 TMB (3,3',5,5'-tetramethyl-[1,1'-biphenyl]-4,4'-diamine) substrate kit, ... -
CASTing Off for New Shores in Human Genome Editing
TypeBlog Post... editing eukaryotic cells — at least, until they met the directed evolution expertise of the Samuel Sternberg... -
Science Careers: Unruly Interests Feed Many Paths
TypeBlog Post...it? how did you get there? Many of those people I met through AWIS (Association for Women in Science); ... and embedding individual diversity to get from gamete to a visibly complex - and unique - adult. My initial... -
Synthesized by Ginkgo Bioworks, Shared by Addgene: SARS-CoV-2 Plasmids for Many Expression Systems
TypeBlog Post...speed while ensuring our rigorous QC standards are met, we are taking a unique hybrid approach compared ...Mediated by SARS Coronavirus Spike Proteins. In: Methods in Molecular Biology. Humana Press, pp 331–338 ...available at Addgene Read about SARS-CoV-2 detection methods Resources on Addgene.org Find all plasmids from... -
A Novice's Perspective on Neuroscience - SfN2017
TypeBlog Post...between neuroscience and other fields. For instance I met one researcher studying how the microbiome can affect...engineering E. coli metabolism, maybe you’ll be able to apply your knowledge of metabolism to better understand...there were also people ready to discuss their VR helmets, gait tracking technologies, and brain monitors...receptors to the brain, and probing how fatty acid metabolites affect brain function. These scientists pulled... -
How to Prepare for an Industry Interview
TypeBlog Post...Industry interviews, however, were more structured. I met with several individual and/or small groups, usually...have a theme too, which is something not inherently captured by the STAR method. I like to think of the ...stories to tell in your interview The STAR interview method is a popular way to answer behavioral interview...situation, task, action, and results. While this method seems simple, I found it hard to remember to describe...interview. Each interview is an opportunity to learn something and then apply it to the next interview. Perhaps...underdressed, but read emails from HR carefully because sometimes companies have relaxed dress codes for interviews... -
Hot Plasmids April 2018 - Protein Degradation, Nanoscopy, FIRE-Cas9, and Yeast Expression Tools
TypeBlog Post...lysosomes. Though a number of biosensors existed that met some of the criteria necessary for studying endosomes...activate the proteasome. Clift et al. tested multiple methods for TRIM-Away delivery. For small cell populations... recent Nature Communications article “SRpHi ratiometric pH biosensors for super-resolution microscopy...be photostable over a broad pH range. The four biometric probes successfully targeted endosomes under a...modules to repress or activate gene expression, sometimes incorporating chemically inducible approaches ... -
Human Germline Editing Using CRISPR
TypeBlog Post...hint of CRISPR editing in human embryos has been met with a storm of media coverage. But the paper published... -
The Future of Research Symposium Boston 2015
TypeBlog Post...Symposium A year ago, trainees from around Boston met to discuss their visions for a successful and sustainable... -
5 Steps to Writing Grant Proposals for Grad Students
TypeBlog Post...Aims section highlights each milestone that must be met in order to deem your proposal successful. Typical..., if not all, of the limitations of current methodologies. This section sets the stage for why your funded... -
Fluorescent Proteins 101: Introduction to FRET
TypeBlog Post...order for FRET to occur several constraints must be met. The donor and acceptor fluorophores must be compatible... determine whether two proteins are within 10 nanometers of each other, or measure the tension in the ...Nicolas. "FRET-based molecular tension microscopy." Methods 94 (2016): 33-42. PubMed PMID: 26210398. Additional... -
Gaining Leadership Skills Volunteering at a Professional Organization
TypeBlog Post...attend the annual NPA meeting to learn more. Having met many people at that conference, I later became involved... -
SciComm: Taking Science to Elementary and Middle Schools
TypeBlog Post...compliment the STEM curriculum. As it happened, I’d met some of the 8th grade science teachers socially and...minds, but I think many of the students learned something. Based on the initial guidelines I received from... -
Transferable Skills Guide: Managing a Team
TypeBlog Post.... I had no idea this was happening even though I met with X on a weekly basis! The silence and maybe even... opportunities. I still need to check myself sometimes to make sure I am delegating well, but it does... -
Experimenting with New Careers while in Grad School
TypeBlog Post...all ages, learned a lot about the dinosaurs, and met wonderful people. When I started volunteering, I ...experience taught me something about myself and my future career. If it wasn’t something I liked – well, I...through developing and leading a budding nonprofit, something I had zero experience with before founding CINSO... -
What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program
TypeBlog Post...Enabling Discovery Through Genomic Tools) Program and I met scientists from 21 different labs who clearly also...access to genotypic study of diverse organisms. Methods for genetic manipulation still need to be developed..., microbes, viruses, and fungi for which these methods are presently unavailable.” As stated by one of... funded labs to rapidly disseminate protocols, methods, data, and tools that result from their work. Researchers...diverse and they inhabit all habitats - even 50 meters from Old Faithful in 60 °C soils. Dendrobatidae...using Xenopus frog cell lines to develop robust methods for frog gene editing. They edit in a cell line...species monogamous. Anolis sagrei (Doug Menke) No methods were available to create targeted gene mutations...