We narrowed to 840 results for: Met;

...will get the research funded,” she says. Baradaran met Addgene Executive Director Joanne Kamens several ...for Biomedical Research. Drug development was something Stegmeier has been interested in for a long time...

...to work! Set up similarly to the online class, we met as a group for sessions covering individual portions... new technique, then type up the materials and methods. Over time that portion of the paper will be generated...

...booming, but, over the years, many researchers we’ve met with have asked if we can help them share and distribute...

...that only further impairs the patient. When I first met AJ and his family, he did not seem much different...

... contribute more. Assuming basic needs are being met (i.e. enough pay and benefits to live on), the factors...Good relationships with clients and colleagues Sometimes this means you have to fire a good performer if...when making decisions, assignments and polices. Sometimes the boss has to be the boss and make difficult...deadlines (there’s that need for flexibility, sometimes taken to an extreme by scientists) Are highly ...

...Overview Quantification Methods Capture Methods Visualization Methods Cell Sorting Methods Multiplex Assays ...Antibody Applications - Cell Sorting Methods Flow cytometry Flow cytometry, used commonly in immunology, allows... through the indirect detection method. In the direct detection method, the signaling molecule is conjugated...Antibody Applications - Quantification Methods Quantification methods, such as western blots or ELISAs, are...output measured. This method can be performed using the indirect detection method via a conjugated secondary...sample. Controls for quantification methods Quantification methods should be run with: a positive control...included. Antibody Applications - Capture Methods Capture methods such as immunoprecipitation (IP) and chromatin...

... Temperature Genetically encoded ratiometric fluorescent thermometer A highly-sensitive genetically encoded...sensors for metabolites like fructose-1,6-bisphosphate , cyclic di-GMP , or S-adenosyl methionine Reporters...Fluorescent Biosensors Fluorescent Proteins: Biosensors Metal Ions Small Molecules Neurotransmitters Cell Physiology...plasmids and AAV Packaged on Request for many more. Metal Ions Target Description Article PI Calcium jGCaMP8...neuronal populations and microcompartments. Nat Methods. 2019 Jul;16(7):649-657. Douglas Kim , GENIE Project...mice with improved GCaMP calcium indicators. Nat Methods. 2009 Dec;6(12):875-81. Loren Looger Calcium NEMO... with large dynamics and high sensitivity. Nat Methods. 2023 Jun;20(6):918-924. Yubin Zhou Calcium Twitch...

...eukaryotic cells. Pairs from methanogens like Methanosarcina barkeri and Methanosarcina mazei are orthogonal ...David Tirrell 63177 pMarsL274G methionyl-tRNA synthetase (L274GMmMetRS) M. musculus azidonorleucine (Anl...Bacterial TAG Ryan Mehl 89189 pMaRSC methionyl-tRNA synthetase (L274GMmMetRS) M. musculus azidonorleucine (Anl...Mehl 163915 pRSF-ChPylTMSK ChPylTMSK N6-(((Trimethylsilyl)methoxy)carbonyl)-L-lysine (TMSK) Bacterial TAG...200226 pMega-MaFRSA PylRS M. alvus meta-trifluoromethylphenylalanine Bacterial TAG Alanna Schepartz 201922...G1 PylRS Methanogenic archaeon Bacterial Ahmed Badran 209739 pAB228i24 G1 PylRS Methanogenic archaeon ...TAG Abhishek Chatterjee 220800 EF1a-L274GMmMetRS-T2A-mCherry MetRS M. musculus azidonorleucine (Anl) Mammalian...

...Using the Indirect ELISA Method Antibody Validation Using the Indirect ELISA Method You may also like... ...of each well at 450 nm on the spectrophotometer. Use the spectrophotometer to generate a standard curve... we list the specific equipment, reagents, and methods that we use in our lab at Addgene. Equipment and...an automated ELISA plate washer. Equipment Spectrophotometer compatible with 96-well plates 1–10 µL single...PBS, 1X pH 7.4, VWR 45000-446 TMB (3,3',5,5'-tetramethyl-[1,1'-biphenyl]-4,4'-diamine) substrate kit, ...

...delivery The first of these methods is RNA delivery of Cas and gRNA. This method involves using in vitro ...endosomal approach. Similar to the mRNA method described above, this method is also transient, as the RNPs will... edit using DNA-based methods like sequencing or digital PCR , RNA-based methods like qPCR , and/or protein-based...protein-based methods like a Western blot . Validation Methods There are several common ways to verify...cells. The system may be delivered using viral methods, as DNA or mRNA encoding the Cas enzyme and gRNA...Delivery Using Plasmids The most straightforward method to deliver a CRISPR system into mammalian cells... efficiently transfect. However, note that this method is most useful for cell lines that you can transfect...

...it? how did you get there? Many of those people I met through AWIS (Association for Women in Science); ... and embedding individual diversity to get from gamete to a visibly complex - and unique - adult. My initial...

... editing eukaryotic cells — at least, until they met the directed evolution expertise of the Samuel Sternberg...

...toxins, cell viability, and tumor metastasis. One of the most popular methods for conducting genome-wide screens...activation through cytosine demethylation DNMT3A — repression through cytosine methylation MQ1 — repression through... to conventional methods for genomic purification, CRISPR-based purification methods are more straightforward... currently no defined measure of fidelity, so sometimes they will be referred to as increased fidelity...Cas9’s proofreading capabilities. No matter the method, increased fidelity enzymes lead to less off-target... editing is a “search and replace” gene editing method in which Moloney Murine Leukemia Virus Reverse ...Although lentiviral libraries are the most popular method for CRISPR screening, they are not suitable for...

...difficult to decide which method to use. Addgene's blog post Delivery Methods for Generating iPSCs provides...combinations A more efficient method to generate integration-free human iPS cells. Nat Methods. 2011 May;8(5):409...an overview of several different reprogramming methods with the goal of helping readers choose a strategy...strategy suited to their research. Once you know which method you would like to use, browse Addgene's collection...cell into another via transdifferentiation. This method of cell lineage conversion can be faster, bypasses... stem cells (iPSCs). Sort the table by delivery method, species, or PI and then select the article link...link to find more information. Delivery Method Species Description Article PI Lentivirus Human Expression...

...pOpen-EcoRIM M.EcoRI DNA methyltransferase 165517 pOpen-Eco31IA Eco31IA DNA methyltransferase 165518 pOpen-NotIM...pOpen-NotIM M.NotI DNA methyltransferase 165527 pOpen-Eco31IB Eco31IB DNA methyltransferase 165536 pOpen-PstIM...pOpen-PstIM M.PstI DNA methyltransferase 165538 pOpen-SfiIM M.SfiI 165538 DNA methyltransferase 165547 pOpen-SapIM1...pOpen-SapIM1 M1.SapI DNA methyltransferase 165557 pOpen-SapIM2 M2.SapI DNA methyltransferase 165558 pOpen-EcoRVM...pOpen-EcoRVM M.EcoRV DNA methyltransferase 165577 pOpen-XbaIM M.XbaI DNA methyltransferase 165578 pOpen-NcoIM ...pOpen-NcoIM M.NcoI DNA methyltransferase 165520 pOpen-T4 PNK T4 PNK polynucleotide kinase 165521 pOpen-lambda red...

...Patrick Daugherty 18084 54856 pBad-mAmetrine1.1 tdTomato-pBAD mAmetrine1.1 (donor) tdTomato (acceptor) FRET...gene biology or engineering metabolic pathways to synthesize a target metabolite, genetic tools like CRISPR-Cas9...within the cell, you will need to tag it with something that makes it easy to see under the microscope...library for the generation of FRET biosensors for ratiometric measurements. The MoBiFc Toolkit (Addgene #1000000222...Caulobacter crescentus , Paracoccus denitrificans , Methylobacterium extorquens Julia Vorholt 84693 pMyNT-kan Acetamidase...pLC290 pR/cmtO p -isopropyl benzoate (cumate) Methylobacterium extorquens Christopher Marx 44448 pLC291 pR... pR/TetO Anhydrotetracycline (aTc) Methylobacterium extorquens Christopher Marx 122635 pPEPZ-Plac Plac...

...criteria have disease-causing mutations in the gene methyl-CpG binding protein 2 ( MECP2 ). MECP2 Rett syndrome...is not clear, however, the ability to bind to methylated DNA and recruit known co-repressors or other ...NID demonstrating the importance of binding to methylated DNA and the recruitment of the NCoR complex for...below: Base Editing RNA Editing CRISPR-based DNA methylation editing system - Shawn Liu and Rudolf Jaenisch...clinical trials (Link opens in a new window) HRV Metrics Toolbox - algorithms for data analysis to calculate...caused by mutations in X-linked MECP2, encoding methyl-CpG-binding protein 2. Nat Genet . 23, 185–188....new window) PMID: 16905679 Cuddapah et al. 2014. Methyl-CpG-binding protein 2 (MECP2) mutation type is ...

...including histone acetylation/demethylation, and cytosine methylation/demethylation....activation through cytosine demethylation DNMT3A — repression through cytosine methylation MQ1 — repression through...through cytosine methylation with improved kinetics LSD1 — repression through targeted removal of H3K4...H3K4 and H3K9 methylation Epigenetic modification is typically targeted to a specific promoter or enhancer...

...lysate method. Purity Purity of AAV preparations is assayed by comparing the relative stoichiometric ratios...Overview of Addgene's viral production methods and quality control for lentivirus and AAV. Viral...Production Viruses are generated using standard methods that have been optimized for each specific virus...Details about our production protocols, titering methods, and quality control are described below. AAV Production...higher than those achieved using the standard qPCR method. Titering by the University of Pennsylvania Vector...gene expression and/or function. These data are sometimes reported or posted on the material page for the...

...speed while ensuring our rigorous QC standards are met, we are taking a unique hybrid approach compared ...Mediated by SARS Coronavirus Spike Proteins. In: Methods in Molecular Biology. Humana Press, pp 331–338 ...available at Addgene Read about SARS-CoV-2 detection methods Resources on Addgene.org Find all plasmids from...