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We narrowed to 172 results for: NAP

Showing: 41 - 60 of 172 results
  1. Deciphering the Mysteries of Behavior with Viral Vectors

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    Blog Post
    ...involved in neurogenesis, neuronal survival, and synaptic plasticity. Several studies have found reduced...stimulation to emulate optogenetic treatment of synaptic pathology." Science 347.6222 (2015): 659-664. ... Van Huijstee AN, Mansvelder HD. Glutamatergic synaptic plasticity in the mesocorticolimbic system in ...
  2. New Tools Enable CRISPRa for Neuroscience Applications

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    Blog Post
    ...kinase II alpha (aCaMKII; excitatory neurons) and Synapsin-1 (pan-neuronal) promoters (Figure 1b), and sgRNAs... Neuron-specific expression is mediated by the synapsin 1 (SYN) promoter, which was superior in its ability...physiology by promoting the development of neurons and synapses as well as long-term memory formation. The Bdnf...
  3. Designing Your Chalk Talk for the Academic Job Interview

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    Blog Post
    ... Many thanks to our guest blogger, Erik Snapp! Erik Lee Snapp received his PhD in Molecular Microbiology...This post was contributed by Erik Snapp, the Director of Student and Postdoctoral Programs at the Janelia...
  4. 9 tips for a successful postdoctoral experience

    Type
    Blog Post
    ... Many thanks to our guest blogger, Erik Snapp! Erik Lee Snapp received his PhD in Molecular Microbiology...This post was contributed by Erik Snapp, the Director of Student and Postdoctoral Programs at theJanelia...
  5. Special Delivery: Fluorophore Targeting for FRET Studies

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    Blog Post
    ...-DnpK dinitrophenyl hapten Mammalian Cells pANAP AnapRS Mammalian Cells Note: This is not an extensive...fluorescently label antibodies, and purification is a snap using thin layer chromatography (Fessenden, 2009...
  6. A Primer on Optogenetics: Introduction and Opsin Delivery

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    Blog Post
    ... a particular neurotransmitter release or post-synaptic current. Fortunately, if your lab focuses primarily...expressing, generic neuronal promoters such as synapsin or camKIIα drive most of the constructs. As an...neuronal death if allowed to express for too long (synapsin was privately communicated to me as one of these...
  7. Antibody Validation for Flow Cytometry

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    Blog Post
    ...400,000 cells were stained with the indicated Synaptotagmin-1 antibodies and corresponding Multi-rAb CoraLite...You can see an example of their work on their synaptotagmin 1 page. The data produced is available on the...to selecting high-performing antibodies for Synaptotagmin-1 (Uniprot ID P21579) for use in western blot...
  8. Top Requested AAV of 2017: pmSyn1-EBFP-CRE

    Type
    Blog Post
    ...encoding Cre recombinase under the control of the synapsin promoter. It can be used to drive Cre expression...AAV. Because Cre expression is controlled by the synapsin promoter, it and therefore mCherry will only be...
  9. New and Upcoming Viral Vectors - September 2019

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    Blog Post
    ...controls: pAAV-hSyn-DIO-EGFP (50457-AAV5): The Synapsin promoter directs broad, neuronal expression. AAV...all our GABA sensor AAV inventory here. pAAV.hSynap.iGABASnFR (112159-AAV1) Optogenetics Optogenetics...
  10. Visualizing Protein Turnover In Situ

    Type
    Blog Post
    ...turnover within a neuron? For my PhD I studied a synaptic protein that is linked to neurodegeneration. The...normal cell function, and, because I was studying a synaptic protein, I wanted to measure it’s levels in its...
  11. Experimenting with New Careers while in Grad School

    Type
    Blog Post
    ... myidp.sciencecareers.org mentioned museums. Indianapolis is home to one of "The world’s biggest and best...children’s museum", The Children's Museum of Indianapolis. I drove passed these dinosaurs every day. They...
  12. Plasmids 101: Degron Tags

    Type
    Blog Post
    ...the presence of a specific protease inhibitor, asunaprevir, the viral protease can no longer self-cleave...components are 1) a SMASh-tagged protein and 2) asunaprevir (small molecule). dTAG The degradation tag (dTAG...
Showing: 41 - 60 of 172 results