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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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Showing: 41 - 60 of 164 results

  1. Lab Automation at Addgene

    Type
    Blog Post
    ...400 vials in 35 minutes compared to a lab assisstant filling about 200 vials in 45 minutes by hand. How ...

  2. Tips for Technical Support Calls

    Type
    Blog Post
    ...number of messages in our thread or remove a good 5 minutes from our phone call. If your concern is based...get. There are questions that are answerable in minutes and questions that could take weeks to resolve ...

  3. Plasmids 101: FLEx Vectors

    Type
    Blog Post
    ...recombinases.   Figure 1 (modified from Schnutgen et al. Nat Biotech 2003): Basic FLEx switch will...population.    Figure 2 (modified from Schnutgen et al. Nat Biotech 2003): In the presence of Cre...

  5. 10 Basic tips for mammalian cell culture

    Type
    Blog Post
    ...air and beyond Turn on the hood for at least 15 minutes before you start any tissue culture work and make... to achieve a consistent growth rate. For every minute spent organizing, an hour is earned (Benjamin Franklin...

  6. Cloning Mammalian Cells with the Agarose Method

    Type
    Blog Post
    ...can pick 48 clones from a 10 cm dish in 15- 20 minutes- a far more rapid pace than using cloning rings...placing on the wrist or cheek. Within less than a minute, when it is only warm but not hot- and still liquid...

  7. Which Fluorescent Protein Should I Use?

    Type
    Blog Post
    ...create the chromophore. This can be from a few minutes after it is translated to a few hours. For example...However, for most FPs it is a few seconds to a few minutes. Photostability can be affected by experimental...

  10. Plasmids 101: Shuttle Vectors

    Type
    Blog Post
    ...the host organism being unable to synthesize a nutrient required for growth. Before delivery of the vector...cells are grown in media that supplies the key nutrient. The vector provides the missing synthesis machinery...

  11. Using Video to Share Your Science: We Share Science

    Type
    Blog Post
    ...WeShareScience is a free platform for sharing short, 3 to 5 minute videos about research. Videos can be shared by ...communicating research. Short videos of 3 to 5 minutes provide more than enough time for researchers to...

  14. 10 Steps to a Perfect Science Talk

    Type
    Blog Post
    ...to see how many in my audiences have given a 50 minute talk (not a lab meeting) in the previous year. ...urge to say everything I allow 1 slide for every 1 minute of talk length, and then I cut a few more slides...

  17. 2018 #DeckTheLab Contest Winners

    Type
    Blog Post
    ...stopped working on our reports/lab stuff for a few minutes to create this lovely winter scene out of stuff...
Showing: 41 - 60 of 164 results