We narrowed to 66 results for: OAT
-
TypeBlog Post...can be officially regulated. I did not try to sugarcoat the potential dangers of people using the technology...
-
Tips for arabidopsis transformation
TypeBlog Post...flowers inside. 50 mL falcon tubes or plastic weigh boats are both great options for this, depending on the... -
Lab to Office Culture Shock
TypeBlog Post...through microscopes, and sometimes you even wear a lab coat! These are the things the rest of the world imagines... -
Antibiotic Resistance: An Old Solution but a New Problem
TypeBlog Post...becomes available. For routine infections, like strep throat, testing for antibiotic resistance isn’t commonly... -
Tips for CRISPR Gene Editing in Mice
TypeBlog Post...are several variants of the CRISPR/Cas9 system floating around, and many pre-designed plasmids containing... -
SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas
TypeBlog Post...primers. These are then immobilized with streptavidin coated beads. Fluorescently labelled dCas9 complexes containing... -
Proximity Labeling: A Powerful Tool for Protein Complex Purification and Proteomic Mapping
TypeBlog Post...proteins that are tagged with biotin using avidin coated purification techniques. Due to the high affinity... -
Plasmids 101: Biotinylation
TypeBlog Post...easily purified using columns or beads that are coated with avidin. The biotin-avidin interaction has... -
Fluorescent Proteins 101: When GFP lets you down
TypeBlog Post...). RNA production can be detected by using a MS2 coat protein that specifically detects a specific RNA... -
Finding Your Perfect Job After University
TypeBlog Post... too easy to see ‘real’ science as wearing a lab coat and working at the bench, but the reality is a little... -
A Guide to Getting Started in Undergrad Research
TypeBlog Post... like Albert Einstein and Bill Nye in their lab coats handling glassware with brightly colored hazardous... -
Antibodies 101: Introduction to Antibodies
TypeBlog Post...antibodies are derived from animals, such as rabbits or goats; the animals are injected with the antigen that ... -
With an Eye Towards the Future, We Look Back at the March for Science
TypeBlog Post...out, marched with Truth. She was a woman in a lab coat. The march opened with speeches but it is how the... -
Selecting Your Plasmid Purification Kit
TypeBlog Post...vacuum. In a bead-based approach, you mix specially coated magnetic beads into your sample and use magnets... -
Optogenetics + CRISPR, Using Light to Control Genome Editing
TypeBlog Post... RNA (gRNA), while the CRY2PHR-activator fusion floats freely, depicted in the figure below. Once triggered... -
Using AAV for Neuronal Tracing
TypeBlog Post...differences in viral uptake by cells and to the uncoating step in the viral life cycle. Using AAV for neuronal... -
Weighing Reagents Protocol
TypeProtocol...prior to handling the materials. Equipment Weighing boats or weighing paper Balance Scoopula or spatula Container...the reagent you will be weighing out, a weighing boat or weighing paper to contain the reagent while on...larger than the capacity of your balance or weighing boat, you can weigh out the reagents in batches. For ...your container after each time. Place the weighing boat or weighing paper onto the balance. These are used...paper could stick to the surface. Tare the weighing boat or paper. You need to do this because you don’t ...t want to include the weight of the weighing boat or paper into your measurement. Hit the tare button ... the material you are weighing onto the weighing boat or paper. If you weighed out too much material, ... -
Antibody Guide
TypeGuide...primary conjugated antibody. Overview of application Coat antigens to the well of a plate, either by charge...] [primary source secondary recognizes], e.g. , “goat anti-rabbit.” Choose a secondary that recognizes... -
Water Bath Protocol
TypeProtocol... Many items may float in the water bath when they are placed inside. Water bath floats can be used to ...Disinfectant Thermometer Water bath weights and floats Reagents None needed Procedure Ensure that the ...on the side of the tube. This way, as items are floating, you do not need to necessarily maneuver the bottles... -
Personal Protective Equipment (PPE) for BSL-1 and BSL-2 Labs
TypeProtocol... for tips on using PPE in the lab. Equipment Lab coat Close-toed shoes Gloves Eye protection and face ...face shields, as needed Guidelines Your lab coat should be buttoned to protect your body and clothing from... gloves in a biohazard waste container. Your lab coat and gloves should not be worn in "common areas" ...