Skip to main content
Addgene
Showing: 41 - 60 of 288 results

  1. Caltech Systemic Capsids

    Type
    Collection
    ...version of the AAV decision tree can be found at the bottom of this page. For more in depth descriptions of...

  2. RUBY-Red Siliques

    Type
    Blog Post
    ...genome. (Bottom panel, right) A highly expressing RUBY plant is a vibrant red/magenta while (bottom panel...developing seeds from a homozygous RUBY plant. (Bottom panels) RUBY expression can vary from different...

  3. Viral Vectors 101: Inverted Terminal Repeats

    Type
    Blog Post
    ...their RBE and T-shaped hairpin loop sequences (Wilmott et al. 2019). Most ITR sequences are from AAV2 ...cross-packaged with capsids from multiple AAV serotypes (Wilmott et al., 2019), and different combinations of ITR...doi.org/10.3389/fmed.2023.1106085. PMID: 36817775. Wilmott, P., Lisowski, L., Alexander, I. E., & Logan, G...

  4. Technical Design of a Western Blot

    Type
    Blog Post
    ...has a less… distinctive smell. BME smells like rotting eggs but has a longer shelf life when stored properly...time; increased reagents Good luck and happy blotting! Many thanks to Addgenies Ashley Waldron, Meghan...blog Antibodies 101: The Basics of Western BlottingAntibodies 101: Selecting the Right AntibodyAntibodies...single protein controls in semi-quantitative immunoblotting. Journal of Neuroscience Methods, 172(2), 250...

  5. Antibodies 101: Stripping and Reprobing Western Blots

    Type
    Blog Post
    ...PVDF) membrane, which is sturdier and retains the blotted protein better than a nitrocellulose membrane. ...secondary antibody. This is the one time in western blotting that you’re looking for a “blank” result! If some...Resources on the Addgene blog The Basics of Western Blotting Find other tips and tricks for molecular biology...

  6. High Complexity Golden Gate Assembly with NEB’s DAD

    Type
    Blog Post
    ..., Ong, J. L., Kucera, R. B., Langhorst, B. W., Bilotti, K., Pryor, J. M., Cantor, E. J., Canton, B., Knight...10.1021/acssynbio.8b00333 Pryor, J. M., Potapov, V., Bilotti, K., Pokhrel, N., & Lohman, G. J. S. (2022). Rapid....1c00525 Pryor, J. M., Potapov, V., Kucera, R. B., Bilotti, K., Cantor, E. J., & Lohman, G. J. S. (2020). ...

  7. Getting to Know Expansion Microscopy

    Type
    Blog Post
    ...and genetically encoded fluorescent proteins (Gambarotto, et al. 2019; Tillberg, et al. 2016). That being..., parasites, and more (Bertiaux, et al. 2021; Gambarotto, et al. 2019; M’Saad, et al. 2020). Plus, it’...https://doi.org/10.1016/j.neuron.2010.11.021  Gambarotto D, Zwettler FU, Guennec ML, et al (2019) Imaging...

  8. Analyzing CRISPR Editing Results with ICE from Synthego

    Type
    Blog Post
    ...clicking the Download Analysis Data button on the bottom right of the analysis screen. ICE analysis details...representation in the edited pool. The black vertical dotted line represents the cut site, and yellow + symbol...with the red underlined PAM. The vertical black dotted line represents the cut site. Cutting and error-prone...

  9. Using Video to Share Your Science: We Share Science

    Type
    Blog Post
    ...in popularity over the last decade. Research by Scott Spicer at the University of Minnesota helps illustrate...about the research is of interest. In his research, Scott Spicer concludes that “… the emergence of the video...numbers." Nature 525.7569 (2015): 306-307. 3. Spicer, Scott. "Exploring video abstracts in science journals:...

  10. Antibodies 101: Reading a Flow Plot

    Type
    Blog Post
    ...realize events are plotted on an invisible Cartesian plane (right.) Each event is plotted by the amount of...antibodies.) Each event read by the machine is plotted as a dot on an X,Y axis to generate a dot plot ...

  11. Plasmids for Stem Cell Research

    Type
    Collection
    ... pii: cells9112451. doi: 10.3390/cells9112451. Ottosson Stem Cell Research Plasmids This table contains...

  12. A History of Genome Engineering in Popular Culture

    Type
    Blog Post
    ...products. But it also made SciFi director Ridley Scott wonder what would happen if these corporations would...genetic engineering to exploit human beings. Ridley Scott directed Blade Runner (1982). It is a dystopian ...00)01250-6 Ran FA, Hsu PD, Wright J, Agarwala V, Scott DA, Zhang F (2013) Genome engineering using the ...

  13. A Needle in a Base-Stack: Cas9 Structural Biology

    Type
    Blog Post
    ... the REC lobe and doubles back on itself at the bottom of the image. Stem loops 1 and 2 extend horizontally...compared to the guide RNA-bound state (PDB ID 4ZT0, bottom), seen from the same angle. Box highlights the ...s41586-022-04470-1 Cofsky, J. C., Soczek, K. M., Knott, G. J., Nogales, E., & Doudna, J. A. (2022). CRISPR-Cas9...

  16. New and Upcoming Viral Vectors - December 2019

    Type
    Blog Post
    ...AAVrg pAAV-mDlx-NLS-mRuby2 112677 AAV1, AAV2 pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP 27056 AAVrg...114471 AAV5 pAAV-Ef1a-fDIO mCherry 112677 AAV2 pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP 50457 AAV2...

  19. New and Upcoming Viral Vectors - September 2019

    Type
    Blog Post
    ...expression in forebrain GABAergic interneurons. pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP (112677-AAV5...) pAAV-hSyn-hChR2(H134R)-mCherry (26976-AAV5) pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP (112677-AAV5...
Showing: 41 - 60 of 288 results