We narrowed to 117 results for: PEP;
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TypeProtocol...Millipore Sigma 12072 Antipain, Millipore Sigma 10791 Leupeptin, Millipore Sigma L2884 Aprotinin, Millipore Sigma...sterilize 1000X protease inhibitor cocktail 25 mg leupeptin 50 mg antipain 250 mg benzamidine 25 mL of 2 mg...
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Neuronal labeling with Spaghetti Monster
TypeBlog Post...neuronal circuits. Epitope tags are short antigenic peptide sequences, attached to a protein of interest (POI...simian virus 5-derived epitope (V5), the synthetic peptide FLAG, the synthetic streptavidin-binding strep-... -
Hot Biosensors 2022: Year-End Roundup
TypeBlog Post...Addgene’s catalog is a new class of sensors for neuropeptides and neurotransmitters. Motivated to develop ...GPCRs recognizing various neurotransmitters, neuropeptides and other ligands - almost A to Z! - from ATP... -
Antibodies 101: Single Chain Fragment Variables (scFvs)
TypeBlog Post...recombinant antibody. They are ~25 kDa single polypeptides that contain the variable light chain (VL) and... two chains are connected by a flexible linker peptide that is usually 15-20 amino acids long and made... -
Using Phosphoserine to Study Protein Phosphorylation
TypeBlog Post...extension and translation of the pSer-incorporated polypeptide. These three components were shown to effectively...proportional to a single phosphate moiety on the peptide of interest. After confirming pSer incorporation... -
27 Hot Plasmids from 2016
TypeBlog Post.... Mark Howarth’s lab recently engineered a new peptide/protein pair, SnoopTag/SnoopCatcher, that can be...link desired proteins together via a spontaneous isopeptide bond. When used in combination with their previously... -
Celebrating the 2024 Nobel Prize in Chemistry
TypeBlog Post...like high resolution Ras sensors and crosslinked peptides proposed as a scaffold for a new generation of... -
Antibodies 101: Affinity Tags
TypeBlog Post...This is where affinity tags come in – they are peptides which do not naturally exist in most expression... -
Viral Vectors 101: Parts of the AAV Packaging Plasmid
TypeBlog Post...type tropism. Introducing changes and/or short peptides sequences into these variable loops is one method... -
Another Pathway into Cells: iTOP
TypeBlog Post...transduction strategy is to fuse a cell penetrating peptide (CPP) to a protein of interest thereby giving it... -
K. phaffii: Rising to the Occasion
TypeBlog Post...of K. phaffii lacks certain terminal mannose glycopeptides (which most other yeast possess) that are problematic... -
New Neuroscience Tool: The iGluSnFR3 Glutamate Sensor
TypeBlog Post...the pMinDisplay backbone is fused to a specific peptide segment (PDGFR TMD) that anchors the sensor to ... -
Visualizing Translation at the Single Molecule Level
TypeBlog Post... translated, the SunTag sites on the nascent polypeptide recruit scFV-GFP, generating green puncta that... -
Antibodies 101: Monoclonal Antibodies
TypeBlog Post...protein. Animals are first injected with a small peptide that represents a short, unique amino acid sequence... -
A Practical Approach to Choosing the B(right)est Fluorescent Protein
TypeBlog Post... the protein of interest by a 2A self-cleaving peptide (Goedhart et al, 2010). An explanation of the assay... -
Antibodies 101: Polyclonal Antibodies
TypeBlog Post...want to generate antibodies against, or a unique peptide sequence derived from this protein. At the same... -
Choosing the B(right)est Fluorescent Protein: Aggregation Tendency
TypeBlog Post...homodimerization tendency. This assay employs a peptide derived from cytochrome p450, CytERM, to direct... -
Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics
TypeBlog Post...any exogenous sequences and regenerates a native peptide bond at the split site. Figure 1: The base... -
RANbodies: Reporter Nanobody Fusions
TypeBlog Post...are secreted from cells due to their IgGκ signal peptide sequence, making them easy to harvest from cell... -
Visualizing Protein Turnover In Situ
TypeBlog Post...protein synthesis: incorporation of puromycin into peptide chains." Proceedings of the National Academy of...