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We narrowed to 117 results for: PEP;

Showing: 41 - 60 of 117 results
  1. Neuronal labeling with Spaghetti Monster

    Type
    Blog Post
    ...neuronal circuits. Epitope tags are short antigenic peptide sequences, attached to a protein of interest (POI...simian virus 5-derived epitope (V5), the synthetic peptide FLAG, the synthetic streptavidin-binding strep-...
  2. Hot Biosensors 2022: Year-End Roundup

    Type
    Blog Post
    ...Addgene’s catalog is a new class of sensors for neuropeptides and neurotransmitters. Motivated to develop ...GPCRs recognizing various neurotransmitters, neuropeptides and other ligands - almost A to Z! - from ATP...
  3. Antibodies 101: Single Chain Fragment Variables (scFvs)

    Type
    Blog Post
    ...recombinant antibody. They are ~25 kDa single polypeptides that contain the variable light chain (VL) and... two chains are connected by a flexible linker peptide that is usually 15-20 amino acids long and made...
  4. Using Phosphoserine to Study Protein Phosphorylation

    Type
    Blog Post
    ...extension and translation of the pSer-incorporated polypeptide. These three components were shown to effectively...proportional to a single phosphate moiety on the peptide of interest.  After confirming pSer incorporation...
  5. 27 Hot Plasmids from 2016

    Type
    Blog Post
    .... Mark Howarth’s lab recently engineered a new peptide/protein pair, SnoopTag/SnoopCatcher, that can be...link desired proteins together via a spontaneous isopeptide bond. When used in combination with their previously...
  6. Antibodies 101: Affinity Tags

    Type
    Blog Post
    ...This is where affinity tags come in – they are peptides which do not naturally exist in most expression...
  7. Another Pathway into Cells: iTOP

    Type
    Blog Post
    ...transduction strategy is to fuse a cell penetrating peptide (CPP) to a protein of interest thereby giving it...
  8. K. phaffii: Rising to the Occasion

    Type
    Blog Post
    ...of K. phaffii lacks certain terminal mannose glycopeptides (which most other yeast possess) that are problematic...
Showing: 41 - 60 of 117 results