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...of our viral vectors blog posts Find tips for preparing AAVs Download our viral vectors 101 eBook Resources...

...CRISPR Plasmids Find More Tools for Combinatorial Engineering on Our SynBio Pages ...

...for positions both inside and outside academia. Inspiring future scientists At Addgene, we’re incredibly... a scientist and what benefits did they provide during your education? Let us know in the comments section...

...experience working on and later chairing the Advocacy Committee. I’m now furthering my leadership experience ...abilities we often hear that scientists should acquire during their PhD and postdoctoral training. Addgene executive...more substantial experience can be achieved by volunteering for a professional organization or a scientific...Vision and Ophthalmology Research (ARVO). Hopefully sharing my experience will show you how you can get started...started on your own development as a leader. Chairing a committee I was Chair of the Advocacy Committee...updated some sections of the NPA’s website. By volunteering, you can gain experience working with other ... my verbal and written communication skills. Volunteering on these committees was real work; meetings ...

... start a peer mentoring group? "What Makes a Good Mentor?" Form Your Own Peer Mentoring Group: A How-...presentation skills (as much as you can), form a peer mentoring group to get real feedback Write whenever you ...How-To Guide for Scientists Download our eBook: Mentoring for Scientists [PDF] ...

...about her mentoring style. Set goals with a mentor: The secret of accountability To make a mentoring relationship...want more great advice on mentoring, click below to download the "Mentoring for Scientists" ebook, which... the fifth and final post in the Addgene Blog Mentoring for Scientists Series. The entire series and additional... If you have been following the posts in this Mentoring for Scientists series, you have: realized the ...adding formality and active goal setting to your mentoring relationships, so that you can reap rewards in...Joanne's Reddit AMA Add a little formality to mentoring Just getting together and chatting with another...about their career path is a great start to a mentoring relationship, but if you don’t take this past ...

...brain at a level of abstraction that enables the engineering of its function." Once scientists can do that...big questions that motivate his interest in neuroengineering, and some of the optogenetic tools that are...are still missing. Optogenetic tools for neuroengineering Addgene: For those who may not be experts, can...set of neurons, maybe we could treat patients suffering from Parkinson's, chronic pain, deafness, or other... neuroengineering...

...article in the Addgene Blog Mentoring series will cover 2 of the 7 mentoring questions I set out to answer...programs A formal mentoring program can be a great way to get started with mentoring. Read this Catalyst...Catalyst Report “Making Mentoring Work” to learn about what makes a good mentoring program. If your organization...also a great place to practice your mentoring skills. Online mentoring can work if both parties are diligent...my upcoming mentoring post in which I describe how to organize a successful peer mentoring circle. Asking...School researcher Connie Cepko to learn about her mentoring style. Mentors at home and away We should all ...organization has a mentoring program, consider tapping into it and helping make it work by being an educated...

...gene expression, reporter plasmids and genome engineering of bacteria....Controlled Expression Reporter Plasmids Genome Engineering You may also like… Genetic Code Expansion Collection...if your proteins of interest interact, they will bring both FPs together and you will be able to detect...Molecular Biology Experiments Return to top Genome Engineering Modifying the bacterial genome by knocking out...investigating basic bacterial gene biology or engineering metabolic pathways to synthesize a target metabolite...CRISPR Plasmids page . Additional Addgene Genome Engineering Resources Learn more about CRISPR with Addgene's...Consortium page contains a variety of reagents for engineering and expressing zinc finger proteins. Addgene ...

... molecular biology technologies: from genome engineering and gene expression to reporter systems. Addgene...biology kit for GoldenBraid cloning and multigene engineering, including CRISPR/Cas9 parts. Golden Gate (GoldenBraid...MoClo Plant Parts III: Transformation & Genome Engineering Kit Collection of MoClo Level 0 parts for plant...biology kit for GoldenBraid cloning and multigene engineering, including CRISPR/Cas9 parts. CRISPR Diego Orzaez...the expression of microRNAs (miRNAs) or small interfering RNAs (siRNAs) to achieve gene silencing in plants...Time with Transient Plant Leaf Transformations Engineering the Plant Genome Using CRISPR/Cas9 CRISPR-mediated...

...thaw of antibodies and suggests preparing single-use aliquots before storing at -20 °C. Each antibody catalog...production, all antibody preps are titered and undergo stringent quality control by Addgene or a trusted quality...distribution. Details about our production protocols, titering methods, and quality control are described below...Coomassie staining, the sample is separated via a denaturing SDS-PAGE gel alongside serial dilutions of a ...each order based on the total items in the order, ensuring each item ships safely and most economically. ...

...Parkinson's Kalle Gehring 45970 pGEX-parkin 77-465 PRKN GST tac Parkinson's Kalle Gehring 45971 pGEX-parkin...Parkinson's Kalle Gehring 45972 pGEX-parkin 219-465 PRKN GST tac Parkinson's Kalle Gehring 45973 pGEX-parkin...Parkinson's Kalle Gehring 45974 pGEX-parkin W403A PRKN GST tac Parkinson's Kalle Gehring 45975 pGEX-parkin...Parkinson's Kalle Gehring 45976 pGEX-parkin C431S PRKN GST tac Parkinson's Kalle Gehring 45977 pGEX-parkin...Parkinson's Kalle Gehring 227353 pGEX-parkin V224A PRKN GST, HRV 3C tac Parkinson's Kalle Gehring 227354 pGEX-parkin...Parkinson's Kalle Gehring 227355 pGEX-parkin R256C PRKN GST, HRV 3C tac Parkinson's Kalle Gehring 227356 pGEX-parkin...Parkinson's Kalle Gehring 227357 pGEX-parkin W403A PRKN GST, HRV 3C tac Parkinson's Kalle Gehring 227358 pGEX-parkin...

...without looking at what they’re delivering and where they’re delivering their payload to.   Figure...anniversary celebration, we’ve been finding and sharing stories unique to our repository and resources....prioritization happens for good reasons. We began offering our viral vector service because we realized that... are available quickly. Beyond the service This brings us to the final piece of the viral vectors service...our AAV Data Hub as a community platform for the sharing of that exact data. Here, requestors can upload...their experimental data in a standardized format, sharing how well a given viral vector worked — or didn’...save time in selecting experimental conditions. Sharing our experiences Addgene’s plasmid repository gave...

...distribution is recovering from the pandemic-related slowdown. More scientists are sharing plasmids and ...are able... Thanks to everyone for being awesome during a tough time. We're all going to get through this...This is a time when we need as much open science sharing as we can get. The effects of Addgene’s brief...Addgene Team because they’re all doing amazing work during this difficult time. One function that rose to ...had to figure out a way to maintain our services during the crisis and maintain our workforce for when ...enough funds to make most of our payroll for the spring and early summer months. We are also extremely ...for grant-driven research being done in all labs sharing materials via Addgene. Perhaps if each of many ...

... a mug or reusable water bottle, you’d probably bring in a mug or reusable water bottle instead of buying...newsletter Help other scientists save resources by sharing plasmids Find plasmids at Addgene ...

... genome engineering in bacteria, alongside more established tools such as Cas9-recombineering, CRISPRa...and bind target DNA sequences using RNA-DNA base pairing. Separately, TnsABC forms a transposase complex... two transposase proteins (TnsB and C). While requiring fewer components for RNA-guided DNA integration...).  CRISPR-transposons for bacterial genomes engineering In the Sternberg group’s initial report of VchINT.... For transposons to be specifically recognized during transposition, genetic cargos must be flanked by...currently studies CRISPR biology and CRISPR genome engineering technologies. Message or follow him on twitter...high-efficiency, multiplexed bacterial genome engineering. Nat Biotechnol. https://doi.org/10.1038/s41587...

...roles. Therefore, institutions are responsible for ensuring that either all affected scientists are paid above...here) pushing for exemptions for postdocs, and lowering of the proposed salary level for exemption. Discussions... are institutions doing? Institutions are now preparing to comply with the changes to the law on Dec 1st...of 340 institutions with postdocs in science, engineering and health from the NSF’s 2014 Graduate Student...University of Delaware has a minimum of $36,360, requiring an increase of 30.6%. We have attempted to check...

...removal helps prevent the invading genome from “recovering” its original sequence. If you’re looking to ... High Fidelty Cas9 Mutants Browse All Genome Engineering Posts Additional Resources on Addgene.org Check... Mary Gearing...

...base pairing, it has promise to be host-independent, beyond the oft published metabolic engineering realm...summarizes Zalatan JG, et al.'s recent paper, "Engineering Complex Synthetic Transcriptional Programs with...of research has shifted in recent years toward tinkering with regulatory networks and multiplex gene control... ‘biofactories’, most successes in metabolic engineering have been limited to the overexpression of various...scaffolds follows the rules of Watson-Crick base pairing, allowing for rational, straightforward design....scRNA design is readily apparent in metabolic engineering. The authors elegantly demonstrated the potential...violacein, a distinctive pigmented protein. By altering the transcriptional programs, the authors sequentially...

...talk, which focused on the history of genome engineering from ZFNs through TALENs and CRISPR-Cas nucleases...huge impact on this field. Advances in genome engineering & CRISPR technology That afternoon's workshop...workshop, entitled "Advances in Genome Engineering", brought us 8 short talks on every conceivable way of examining...more parts-oriented synthetic biology. I've been answering biology customer service questions for nearly ...scientists are tackling this type of genetic engineering is a testament to how amazing the downstream ...downstream payoff could be. Though I attended an engineering school as an undergraduate, I never fooled myself ... The talks we heard Monday night represented engineering first and foremost, from the custom software ...