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Showing: 41 - 58 of 58 results

  6. Zhang Lab CRISPR Page

    Type
    Collection
    ...The PX552 plasmid (#60958) contains pAAV-U6::sgRNA(SapI)_hSyn-GFP-KASH-bGH (SpGuide acceptor). This is a...targeted plasmids. The plasmid can be digested using SapI, which will reveal sticky ends to enable the rapid...

  9. Antibodies 101: Flow Cytometry

    Type
    Blog Post
    ...permeabilization with detergents such as triton-x, saponin, or tween. Take care when using strong detergents...

  10. Arf GTPase Family

    Type
    Collection
    ...) GAP Arfgap3 26286 516 GAP Asap1 5080 1129 GAP Asap2 8853 1006 GAP Asap3 55616 903 Bacterial (pET) GAP...

  11. Worm Expression Resources

    Type
    Collection
    ...along with links to protocols, can be found below: SapTrap-SEC kit for C. elegans genome engineering - Bob...kit can be found at the Goldstein Lab website . SapTrap CRISPR/Cas Toolkit - Erik Jorgensen Lab. A collection...Efficiency Fluorescent Tagging of Endogenous Genes with SapTrap Even more elegant: Single injection CRISPR/Cas9...

  12. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ...none S. pyogenes Gersbach PX552 60958 Mammalian/AAV SapI none S. pyogenes Zhang pgRNA-humanized 44248 Mammalian...backbone)-pCBh-Cre-WPRE-hGHpA-ITR 60229 Mammalian/AAV SapI none S. pyogenes Zhang AAV:ITR-U6-sgRNA(backbone...2A-EGFP-KASH-WPRE-shortPA-ITR 60231 Mammalian/AAV SapI none S. pyogenes Zhang AAV:ITR-U6-sgRNA(backbone...pEFS-Rluc-2A-Cre-WPRE-hGHpA-ITR 60226 Mammalian/AAV SapI none S. pyogenes Zhang pLKO5.sgRNA.EFS.GFP 57822...

  13. 15 Hot Plasmids from 2017

    Type
    Blog Post
    ...compatible with two-photon microscopy using Ti-Sapphire lasers. pBAD-RDSmCherry1 is a red-shifted variant...

  14. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...Yuste Voltage Ultrafast fluorescent voltage sensor ASAP2f Subcellular Imaging of Voltage and Calcium Signals...sensor for detection of single action potentials (ASAP1) High-fidelity optical reporting of neuronal electrical...

  15. Immunology Research Plasmids and Resources

    Type
    Collection
    ...DSHP, EBVS, FLJ18687, FLJ92177, IMD5, LYP, MTCP1, SAP, XLP, XLPD SH2D1B SH2 domain containing 1B EAT2 SH3BP2...ARTS-1, ARTS1, ERAAP, ERAAP1, KIAA0525, PILS-AP, PILSAP ERAP2 endoplasmic reticulum aminopeptidase 2 FLJ23633... defensin, beta 4 DEFB-2, DEFB102, DEFB2, HBD-2, SAP1 EDN1 endothelin 1 ET1, HDLCQ7 EDN2 endothelin 2 ... defensin, beta 4 DEFB-2, DEFB102, DEFB2, HBD-2, SAP1 DKK1 dickkopf homolog 1 (Xenopus laevis) DKK-1, ...

  16. Guide to Using Pooled Libraries

    Type
    Guide
    ...and control cells Generate a list of gRNAs that disappear with addition of the selection mechanism Positive...

  17. Plasmid Collections

    Type
    Collection
    ...established a common place for deposited plasmids. ASAP Discovery Consortium ARF GTPase Family BioBricks...
Showing: 41 - 58 of 58 results