...assay (such as Sanger sequencing). Click here for a useful reference. I have used 200ng to 500ng of 293FT ...

... gRNA in a wide variety of mammalian cell lines Useful for difficult to transfect cell types, can also...

...invaders, and this system is proving to be just as useful (if not more so) for research applications, eclipsing...

...target site to examine for scarring. This may be useful to characterize the non-deleted allele in monoallelic...

...gRNA Design Tools CRISPR Blog Posts CRISPR is a useful tool for genetic screening experiments, due to ...

...Addgene's guide to plasmids and strains useful for expanding the genetic code in bacteria and mammalian...

...AAV vector for nuclear SYFP2 labeling in neurons. Useful for nuclear isolation and scRNA-seq applications...

...will be most useful for achieving your goals. However, you should have a good mixture of useful tasks that...but moderately useful tasks at the expense of time spent on the long and highly useful tasks. The quick...need of a few ideas, I hope you’ll find this post useful. After a bit of research and thinking about my ... should be thinking about two main things: How useful the task is (its utility) How much time it will...that will take longer. Completing the quick and useful tasks will swiftly give you the wonderful feeling...when I write them down. Some people also find it useful to write out their to-do lists at the end of the...time I have blocked off for each project and how useful each item is. For instance, if I don’t have draft...

...DKFZp434N1928, FLJ35755, IL-17RD, IL17RLM, MGC133309, SEF IL17RE interleukin 17 receptor E FLJ23658, MGC71884...

... the block? The Cas family of proteins has many useful genome engineering enzymes, each with their own... RNA instead of DNA. This makes it particularly useful in generating transient edits of signaling molecules...of Cas13 makes it perfect for targeting mRNA and useful as a mechanism of transient knockdown of gene expression...very similar to Cas13 and has been proposed to be useful for the same applications (RNA knockdown, RNA editing...abilities of the other Cas family members make for useful implements in the scientist toolbox. If your genome...

...engineered mouse models provide researchers with useful and adaptable tools to perform basic and preclinical...reproducibility of experiments. Inbred mice are particularly useful for understanding a specific effect related to ...the human population. Outbred strains of mice are useful for comparing dominant and recessive traits and...their impact on diseases or treatments, but are not useful for genetic engineering. Examples of outbred strains...insertion. However, CRISPR/Cas9 systems are more useful for generating knockout mice. Knockout mice Knockout...

... its atomic coordinates. These wire models were useful in that they not only traced the 3-D path of the...believing. And in that sense, models can be both useful, and dangerous. Subscribe to CRISPR Blog Posts ...new book A Crack in Creation. Making models more useful through flexibility It is common knowledge in the...community that All models are wrong….Some models are useful. While a physical model of a protein might represent...structure. While this first Cas9 model is certainly useful, it is also wrong in at least one serious way. ...

...Lentiviruses are useful and efficient tools to introduce your gene of interest into cells. Unlike gamma-retroviruses...most frequently asked lentiviral questions. This useful resource from Addgene Depositor (and lentiviral...specifically for transient expression (find some useful backbones here) or from viral transduction. While..., transient expression of viral constructs is a useful tool as it provides a means to quickly check that...transduction to a particular cell type or tissues; this is useful in gene therapy applications where a distinct subset...

...plasmids in the Addgene catalog would be broadly useful to the research community. It does, however, necessitate...necessitate us first identifying broadly useful vectors, a process that isn’t as simple as it seems. Take...piece of the viral vectors service puzzle: the usefulness of each viral prep in practice. The number of...shows how many scientists believe a vector would be useful in their experiment…but, even for our blue flame... it doesn’t actually show you if the vector was useful for the requestors or how it performed. Addgene...

...Zebrafish (Figure 1) were initially recognized as a useful vertebrate model based on their straightforward... to help parse and visualize expression data in useful ways. Zebrafish mutants In the 1990’s, two revolutionary...organisms by introducing foreign DNA into the genome is useful for a variety of applications. For example, specific...highlight some of the features that make zebrafish useful, I’ll outline two recent lineage tracing approaches...resources and transparency of the embryo—have proven useful in a variety of research applications. Lineage ...

...glycosylation patterns. Surface labeling is particularly useful for classifying and sorting cells by identifying...surface labels. Here, I will introduce you to a few useful labeling and staining techniques beyond standard... for antibodies to enter the cell (Figure 3). A useful side effect of the fixing/permeabilization (fix...maintaining a clean cell surface, dump gating is a useful way to get rid of undesired cell populations. Dump...not on antibodies but on small molecule dyes, are useful, most notably when it comes to viability and proliferation...

...apps you’ve found useful in the comments section to make this post even more useful to your colleagues...second look. 5 Great Apps for Lab Life This very useful post was contributed by guest blogger Sean Stacey... technique to screen for proteins that could be useful for a wide variety of reasons. 3) I would love ...

...well-annotated collection of genes that can provide a useful starting point for developing a model and an assay...pooled library. The individual plasmids will be useful when screening for phenotypes, such as cell-non-autonomous...pooled setting. The individual plasmids will also be useful for imaging or gene expression assays necessitating...biological community will find these reagents to be useful. Happy screening! Many thanks to our guest blogger...

...the necessary expertise to clone them, it can be useful to produce cell lines that overexpress the target...in a given cell line. For flow cytometry, it is useful to produce a sample with mixed expression, with...Cell Differentiation Molecules (HCDM) can be quite useful. HCDM, among other things, works to test flow ...providers for the same clone. This can be particularly useful when designing larger multicolour panels, where...is suitable for your flow experiment. We find it useful to start with a screening procedure in easily genetically...

...they mean is no small task. As a scientist, it is useful to make literature searching and reading a regular...long list of titles for something that is actually useful to you. When embarking on a new literature search...paper-reading, even starting out at 1 paper/week will be useful. Check out the hashtag #365papers on Twitter for...own digital library for the full-text version of useful papers. For many, this collection is purely electronic...