We narrowed to 91 results for: SHR

1. Hot Plasmids - September 2019 - CRISPR, Auxin Inducible Degradation, MerMAID Channelrhodopsins, and Genome Looping

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   ...CRISPRCas9 by engineering SpCas9 Article contributed by Shreya Vedantam CRISPR/Cas9 is widely used genome editing...

2. Viral Vectors 101: Gamma-Retroviral Packaging Systems

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   ...transfer plasmid encoding a transgene, sgRNA, or shRNA of interest: The transgene sequence is flanked by...

3. Viral Vectors 101: Viral Vector Elements

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   ...construct, to cells/organisms. This is often cDNA, shRNA, or something similar, and is contained on the transfer...

4. Synthesized by Ginkgo Bioworks, Shared by Addgene: SARS-CoV-2 Plasmids for Many Expression Systems

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   ...Last updated Jul 20, 2021. By Will Arnold and Shreya Vedantam Accelerating research by improving the ...

5. Enabling Precision Functional Genomics with the Target Accelerator Plasmid Collection

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   ...Variants Identifies Oncogenic Alleles. Kim E, Ilic N, Shrestha Y. et al. Cancer Discovery 2016. PubMed PMID: ...

6. Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME

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   ...response has been a longstanding challenge. Using shRNA, TALEN, or ZFN to edit immune cells poses challenges...

7. Addgene: The First Twenty Years

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   ...beginning Let’s take a quick flash back to 2004. “Shrek 2” was the most popular movie in theaters, the Razr...

8. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

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   ...encoded voltage indicator Article contributed by Shreya Vedantam Genetically encoded voltage indicators...

9. Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins

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   ...voltage indicator Archon Article contributed by Shreya Vedantam  Listen to the voltage indicator podcast...

10. New Tool for Lineage Tracing: The ClonTracer Library

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    ...protocols for in vivo screening with lentiviral pooled shRNA libraries. Later, Bhang says, “When we developed...

11. Progress Towards a PAM-Free CRISPR

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    ...location or efficiency. If you are just trying to shred a long non-coding RNA, cutting most anywhere within...

12. CRISPR in the Clinic

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    ...currently in clinical trials includes Cas3 (the shredder member of the Cas family) as part of a cocktail...

13. Viral Vectors 101: Virus Safety

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    ... GFP. Similarly, if the viral vector carries an shRNA to a gene essential for cellular function, this ...

14. Quickest Way to Deposit Plasmids: The Deposit Spreadsheet

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    ... Choose from: Encodes one insert, encodes gRNA/shRNA, or empty backbone. Purpose Answer “What does...

15. Getting Started with Behavioral Neuroscience: Standardizing Experiments and Using Viral Vectors

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    ...sequence targeting the gene is cloned into a viral shRNA expression vector and the vector particles are delivered...

16. Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!

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    ...matching your search terms with gene and guide RNA / shRNA inserts Resistance Marker: This indicates the selectable...

17. Your Lentiviral Plasmid FAQs Answered

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    ...variety of applications including cDNA expression, shRNA-mediated knockdown, Tet and Cre-regulated expression...

18. New CRISPR Tools: Cas7-11 and PASTE

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    ... were reported as up to 85%, broadly similar to shRNA and Cas13 systems in a range of mammalian cell lines...

19. Overcoming the AAV Size Limitation for CRISPR Delivery

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    ...Cas9 must be made smaller. Previous attempts to “shrink” Cas9 include the use of St1Cas9 (~3.3 kb) from...

20. What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program

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    ... less well known fungi (they are not yeast or mushrooms) but there are over 1,000 species. No transformation...