... available from Addgene as pools provided in two sets, each with 4 gRNAs per kinase, so screens can be...do not lend themselves to screening in a pooled setting. The individual plasmids will also be useful for...individual plasmids separately. Additionally, this set can be used for cherry-picking individual gRNAs that...targeting the mouse and human kinomes. While any subset of the genome is limited by definition, the kinome...plasmids contain the same 3-4 gRNAs found in the first subset of the pooled library. The individual plasmids ...

...single gene, as well as a target site common to a set of similar sequences. Organisms include human, mouse...cloning All-in-one plasmid containing expression cassette for NmCas9 and BsmBI site for cloning in gRNA:...

... and career fit. Most internships share a common set of criteria such as citizenship status, academic ...letter of support.        5. Application submission  Set a calendar reminder for every deadline and shoot ...an in-person interview. Formulate and practice a set of common questions with a peer or mentor. Several...prepared mind” by Louis Pasteur These tips are a set of common sense approaches that will prepare you ...

...of cellular targets by: Mike Lacy Adding to their set of anti-HA frankenbodies (Zhao et al. 2019), Tim ... Hiroshi Kimura’s labs have recently published a set of anti-FLAG frankenbodies, a flexible, modular tool...cells, and use it in a variety of applications. This set of new anti-FLAG frankebodies includes mEGFP, mRuby2...expression vectors (3570 lanes/50 mLs) coexpressing a set of either 10, 30, 50, and 100 kD proteins or 20, ...Every few months we highlight a subset of the new plasmids and viral preps in the repository through ...perform multiplex editing within a single expression cassette is advantageous to Cas9’s larger expression requirements...2: comparison of Cas 9 vs. Cas 12a expression cassettes when encoding multiple guides. While two guides...

...participated in our annual #Deckthelab contest. The bar was set very high from last year’s impressive entries, but...hoarding used tip boxes for a lab supply tree and set a challenge for her lab mates to create the best-...

... if there is statistical significance among data sets. Significance is a numerical value which quantifies... about outliers or data spread within those data sets. Measures of dispersion – standard deviation/.... 2). Assumptions – characteristics of a data set; the ‘rules’ a statistical test assumes your data...parametric tests. Parametric tests are suitable for data sets that “play by the rules” and which we have certain...example, if an ANOVA analysis on a five-sample data set yields statistical significance, you won’t know if...comparisons are performed. For example, if your alpha is set at .05, then 5% of the time the statistical test ...

...meetings is to give honest, constructive feedback, set tangible research and career goals, and celebrate...techniques to finally figuring out a tough experiment, sets a positive and encouraging tone for the meeting....overwhelmed or confused about what’s coming next. Goal setting and career planning help alleviate the anxiety ...suitable careers based on your current interests and skillset. With this information in hand, you can then formulate...

...suggestions into a final version of the document. Set and manage expectations When the researchers involved...Therefore, it’s crucial that everyone meets early on to set expectations for the output that will come from the...other accountable to these expectations once they’re set by checking in about them at your recurring meetings...

...easy to pipette samples in and out of it, you can set up a procedure for it using the Biomek FxP. For example... example, we added a cooling block to our set up. The cooling block is used to keep our cells from warming...transformations in the same amount of time by hand! Setting up a basic protocol on the Biomek FxP is pretty...the shortest amount of time. Racks of 48 vials are set up on the bed of the Liquid Handler and we program...

...festivals. Or engage with non-scientists in an unusual setting: Discuss your latest research in a local pub at...yourself. Pick a topic that is close to your heart, set up a blog using a site like WordPress and start writing...involve the public in your research projects, you can set up a citizen science project. Answering the big science...Museum has downloadable resources to enable you to set up your own citizen science project. Science communication...

...took some playing around to figure out all the settings and tool options, but I found Vectr to be fairly...Biorender watermark, which is usually ok for informal settings like lab meeting presentations. You can download...entire internet for scientific graphics. The tools settings let you tailor your search to particular types...

...LEGO laboratory sets so we had to purchase the big LEGO classic box, the Big Bang Theory set, and then use...potential is endless One night, while opening a LEGO set with my husband, I thought about how the limitless...

...cytometry to analyze your samples, it is necessary to set up a sequence of gates to be able to select and precisely...cell or on its surface. Bear in mind, the voltage setting of the laser impacts the magnitude of FSC, SSC,...cytometer's software will allow you to adjust this setting as needed. To figure out the appropriate voltages...default, a sample on the flow cytometer (before you set the first gate) will be represented through FSC-A...-A vs. SSC-A, so most people use that setting. Figure 2: As the cell passes through the laser beam...

...biological sciences—whereas industry postdocs have a set time limit. At AstraZeneca, this is three years. ...easier to make that transition from an academic setting—especially if job applications require teaching...There are “difficult personalities” in every work setting and no manager will ever be perfect, but the chances...good manager do tend to be higher in an industry setting. Scientific community and mentorship: As an industry...

...relative to a 'normal' sample with known copy number set as the expression baseline. Copy number is then determined...sample relative to your normal control - you can set up your calculations so that your assay output number...numbers should be as small as reasonably possible and set using the variance seen within multiple runs of the...CAR15.) Each droplet contains a primer and probe set for both the gene of interest and the reference sequence...

...Differentiating male and female flies is essential for setting up genetic crosses. At the end tip of their abdomens... hatching from their pupal case (~9 days after setting up the genetic cross). You can tell when pupae ...Animals are diploid except for their germ cells (sets of genomic material are contributed by mother and...meiosis. This can sometimes be a handy feature when setting up genetic crosses. More importantly, this knowledge...

...since primers are insert dependent   PCR set-up Setting up colony PCR reactions is nearly identical...primers to detect the presence of your insert; 2) set up a standard PCR reaction (primers, dNTPs, polymerase...antibiotic for selection.   Lysing bacteria and setting up PCR reactions: The remaining bacteria-water ...know there could be something wrong with the PCR setup and/or the primer design. Use a negative control...

...she's learned to prioritize projects, and how she sets metrics for success. The importance of thinking ...of recognizing and leveraging your unique skills set while also adapting to needs as they arise. Tips ...

...right corner of PubMed to navigate to your account settings. Next, click on the link in the “NCBI Site Preferences...badges you can add and use your new PubMed account to set-up emails to alert you when papers are published ...

...to NHEJ knockout. Base editors can make a limited set of mutations. Repress or Interfere (CRISPRi) Reduce...