We narrowed to 614 results for: TIL

...on the chilled 96-well freezer block in the BSC until ready to use. Prepare the Master Mix Place a ddPCR...pipette tips directly in the center of the well and tilt to a 45° angle. Count to 20 while slowly and gently...Lift the tips ~1 mm. Touch the side of the well and tilt the pipette tips at a 45° angle. Count to 20 while...

... used for ddPCR immediately or stored at -20 °C until ready to use. Preparing for ddPCR Thaw samples, ...pipette tips directly in the center of the well and tilt to a 45° angle. Count to 20 while slowly and gently...Lift the tips ~1mm. Touch the side of the well and tilt the pipette tips at a 45° angle. Count to 20 while...

...recently deposited 2 Bacillus subtilis libraries with Addgene – the B. subtilis Single Gene Deletion Library—Kanamycin...target specific neuronal subtypes was not available until now. In order to target specific neuronal subtypes...to light. Robert Campbell’s lab illustrates the utility of PhoCl in a variety of settings. For instance...the podcast Segement on the pPSU Plasmids B. subtilis knockout and knockdown libraries The Gross Lab... Essential gene Knockdown library. Although B. subtilis has a fully sequenced genome, many applications... to advance our collective understanding of B. subtilis gene functions and interactions. The single-gene...single-gene deletion library is derived from the B. subtilis 168 strain and contains knockouts for all 3984 genes...

... of the agar and gently spread over the surface until the liquid is mostly absorbed (there is a very small...onto the agar and gently spread over the surface until the liquid is mostly absorbed. The spreading of ...

...break through the resolution barrier while still utilizing fluorescence microscopy, is another option....more accessible imaging equipment, ExM protocols utilize relatively inexpensive reagents and are readily...fluorescent proteins (Gambarotto, et al. 2019; Tillberg, et al. 2016). That being said, the anchoring,... between them to ensure the expanded images are still a good representation of reality. Third, expansion...secondaries, can improve signal strength (Sherry and Stiles, 2022). Fourth, signal continuity can appear reduced.../doi.org/10.1371/journal.pbio.3001020  Chen F, Tillberg PW, Boyden ES (2015) Expansion microscopy. Science...https://doi.org/10.1098/rsta.2021.0110  Sherry DM, Stiles MA (2022) Improved fluorescent signal in expansion...

...will remove bases from the 3' end of the cut site until the first G is reached (shown in blue), at which...causing the enzyme to perform exonuclease activity until the first "G" in the sequence. Because the polymerase...

...within an E. coli cell, but vectors can be widely utilized across many different cell types and each one ...can post-translationally modify proteins yet they still share many of the same technical advantages that..., a well-defined genetic system, and a highly versatile DNA transformation system.Unlike most other microorganisms...of these vectors. S. pombe plasmids oftentimes utilize an ARS to aid in high transformation efficiency...markers for yeast Historically, scientists have utilized auxotrophic selection rather than antibiotic selection...auxotrophic strain/ selection element pairs must be utilized or a new combination needs to be created in advance...improvements in antibiotic selection have made utilizing the more traditional drug selection methods feasible...

...water. While stirring, slowly add hydrochloric acid until the solution clears. Check the pH of the solution...dislodge the cells. Incubate the cells for 18 h, or until the following morning. The following morning, carefully...

...have waited. Both tasks have some utility, but I lost all the utility (fun) of eating with friends by splitting... meeting up with friends, but didn’t realize it until it was too late. I didn’t spend enough time thinking... two main things: How useful the task is (its utility) How much time it will take you to complete the...should err on the side of things that have the most utility; i.e. those things that will be most useful for...option. Even if the full experiment fails, you’ll still have made small accomplishments each day that should...and think about what they mean in terms of the utility of your tasks. In my cell splitting anecdote, I...hood may have prevented me from splitting cells until late in the day anway. There are a few things I’...

...FLEx vector design Recombinase-dependent vectors utilize pairs of specific sequences, lox (recognized by...expression, low levels of spurious expression were still observed in many systems. So what is the cause of...of DIO/FLEx vectors, the cause of these events, until recently, was not known. A recent paper from Ed ...post-translational modifications, and other factors that AAV utilizes to gain entry into the cell (Dudek, AM. 2018)....in order to minimize off-target expression while still achieving adequate expression in your cells of interest...recombination rate (0.1% and 0.13% respectively) still required a 200-300 fold dilution to minimize off-target...accuracy and reproducibility of the data and can instill greater confidence when interpreting the outcome...

...water. While stirring, slowly add hydrochloric acid until the solution clears. Check the pH of the solution...disturb the cells. Incubate the cells for 18 h, or until the following morning. The following morning, carefully...

...joining the conversation on Reddit, but it wasn’t until one of our blog writers posted his article (Developing...00 am and Joanne didn’t have to start answering until 5 pm). The ability to see the questions first makes...that I didn’t have the direct link to send people until the day of the AMA. This made it a little difficult...alter my intro to reduce the number of potentially hostile posters (this advice included leaving out the term...career path development--but the science trainees still have to lead on their own development. Please see...early in the day. Although I didn’t start answering until 5pm, frankly, I was typing far before five. Fortunately...your own professional development etc. database. Still have questions? I am easy to reach and happy to ...

...large container holding a small volume of liquid, tilt the container holding the liquid so that it's easier...second stop to release the liquid. Pro-Tip Dispense until the first stop, wait, and then dispense to the second...

... engineering. When compared to that collective utility, it’s easy to fault the lack of features in a single... can be used to aid design. These databases are still young and will require a collaborative effort from...arbitrary PAM definitions. These newer tools may still prove somewhat useful for the latest NTT targeting...protein, Cpf1. Separately, on-target efficacy is still a concept that only a few tools are trying to solve... workbenches such as MacVector or Benchling. Utility functions: These functions help speed up the sgRNA...single-method design”. Trends: The most common utility functions are batch design or multiplex features...use. The R based package CRISPRseek offers great utility and coupled with Bioconductor should be the first...

... with the recipient plasmid's MCS. However, you still need to avoid restriction enzymes that cut within...colonies are a result of uncut empty plasmid, you will still have colonies when you do not add ligase. If the...

...don't reflect true biology. Fluorescent fusions utilizing bulky tags can disrupt normal cell biology and...knock-ins maintain the native protein context, but they still depend on antibody-based detection or western blotting...senescence, and apoptosis. Work by Kamio et al. (2025) utilized HiBiT to explore TP53 biology. They first identified...extracellular RNA editors Tracking factors that utilize exosomal transport is quite challenging. Work by...permeability of the LgBiT protein, it only binds HiBiT still present on the cell surface. EGF treatment resulted...trafficking of the receptor. Peach et al. (2021) utilized HiBiT to address these challenges, exploring the...Engineered luciferase reporter from a deep sea shrimp utilizing a novel imidazopyrazinone substrate. ACS Chemical...

...the mandate has been removed, institutions should still go ahead with raises, as for years there have been...planning to raise salaries prior to the injunction are still honoring these plans. We are now collecting data...following the injunction, 59% of all postdocs are still expected to receive salary raises - down from the...to get a salary increase prior to the injunction still won’t after the injunction (see below). Of the ...salaries, the only postdocs we knew for certain to still be getting their raise were: postdocs at institutions...University of Louisville in Kentucky, the raises are still going ahead as planned. As we are receiving data...

...from one cell to another). Bacteria need special fertility genes to be capable of conjugation, and these ...penicillin resistance plasmid can grow in spite of a hostile Penicillium fungus trying to kill them off. While...possess plasmid-borne toxins like colicins and subtilisins to kill other bacteria in the area, freeing up...systems that allow tuning of metabolic pathways. Fertility (F) plasmids: microbial sharing F plasmids contain...shifted over the years, and new classifications are still being created as whole-genome sequencing becomes...Gateway cloning relies on genes found on an E. coli fertility plasmid that also encodes a toxin, CcdB, and the...

...Transformation Summary PCR based cloning is incredibly versatile and allows for nearly any piece of DNA to be placed...colonies are a result of uncut empty plasmid, you will still have colonies when you do not add ligase. If the...

...the sample concentration is still too low, repeat the centrifugation until the volume of the sample is...