Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

We narrowed to 172 results for: TOX;

Showing: 41 - 60 of 172 results

  1. Cre-lox system

    Type
    Collection
    ...low levels to reduce toxicity; See PI page for other recombinases that are less toxic in Drosophila hsp70...recombination is a very useful tool, Cre expression can be toxic in some systems, notably Drosophila. Read Addgene's...

  2. Lentivirus Plasmids

    Type
    Collection
    ...particle) and toxicity of the gene product (i.e., if the gene encoded by the transfer plasmid is toxic to mammalian...

  3. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ...apicomplexan organism Toxoplasma gondii to the infection of human fibroblasts. Toxoplasma mutants were generated...Mülleder M, et al. F1000Res. PubMed PMID: 27830062 Toxoplasma CRISPR knockout pooled library Parasites from... and livestock diseases such as malaria and toxoplasmosis. Despite their importance to global health, ...

  6. Tetracycline Inducible Expression

    Type
    Collection
    ... may be needed. Or, when inducing a potentially toxic protein you may need extremely minimal background...and tTA. Dox has good tissue distribution, low toxicity, a known half-life (24 hours), and is relatively...

  8. Plasmids 101: Origin of Replication

    Type
    Blog Post
    ...want to make a lot of your plasmid? Is the gene toxic in high amounts? It is always good to keep in mind...they contain large inserts or genes that create a toxic product. The E. coli strain Most E. coli strains...

  9. Top Requested Lentivirus and AAV of 2016

    Type
    Blog Post
    ...using this plasmid Cas9 expression is typically cytotoxic, which makes generation of high titer Cas9 lentiviruses...lentiviruses challenging. This cytotoxicity also causes growth rate variability when using lentiCas9-blast...

  10. All in a Twist: dsRNA

    Type
    Blog Post
    ...advancement over previous chemical versions with toxicity to many organisms. Fig. 1: Exogenous sources... to local dsRNA production. In the same vein, genotoxic stress, such as chemotherapy treatment or ionizing...

  13. Plasmid Tools for Microbiome Studies

    Type
    Blog Post
    ...demonstrated that it can also detect expression of toxin mRNA from Clostridium difficile. As mRNA expression...methods, the ability to detect mRNA transcripts of toxins and other transcripts of interest is a benefit ...

  14. General Transfection

    Type
    Protocol
    ...µg of DNA into 500 µL of Opti-Pro SFM. Pro-Tip Endotoxins can inhibit transfection; therefore, plasmid ...plasmid DNA purification should include an endotoxin removal step. For high quality plasmid DNA, the plasmid...

  15. Hot Plasmids February 2024

    Type
    Blog Post
    ...cells remain difficult to genetically edit due to toxicity from electroporation or poor cellular entry with...frequency (nearly 100% in some cases) and minimal toxicity, including in clinically-relevant lines such as...

  16. Plasmids 101: Protein Expression

    Type
    Blog Post
    ...extremely useful if a desired protein product is toxic to cells at high concentrations. Despite their advantages...lower expression may be required if your protein is toxic to E. coli. Of all the vectors, pET, under the control...

  17. Lentivirus Production

    Type
    Protocol
    ... optimized for each transfer plasmid. Pro-Tip Endotoxins can inhibit transfection; therefore, plasmid ...plasmid DNA purification should include an endotoxin removal step. For high quality plasmid DNA, the plasmids...

  18. Your Lentiviral Plasmid FAQs Answered

    Type
    Blog Post
    ...increased particle stability; however, the protein is cytotoxic precluding the long-term expression of lentiviral...vectors in producer cell lines. Identifying less toxic envelope proteins or envelope proteins that target...

  19. Plasmids 101: Control Plasmids

    Type
    Blog Post
    ...correct? Is the purified plasmid DNA viable? Or cytotoxic? How and where did things go wrong? The use of...reagents or the transfection process itself has any cytotoxic effects on the target cells. Another type of transfection...

  20. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

    Type
    Blog Post
    ...produce viral proteins and die, either through cytotoxicity or the immune response. The second strategy ...expression. In cell lines that can produce HIV-1 toxic proteins, CRISPR/Cas9 SAM caused apoptosis, indicating...
Showing: 41 - 60 of 172 results