We narrowed to 888 results for: TRI

...BGHpA (Alias: CN5140) Expression of CoChR-EGFP in striatal cholinergic neurons Jonathan Ting Viviana Gradinaru...BGHpA (Alias: CN4825) Expression of iCre(R297T) in striatal medium spiny neurons Jonathan Ting Viviana Gradinaru...protein) plus separate nuclear FLAG epitope in striatal cholinergic neurons Jonathan Ting Viviana Gradinaru...BGHpA (Alias: CN4361) Expression of jGCaMP8s in striatal indirect pathway medium spiny neurons (D2 MSNs...BGHpA (Alias: CN4360) Expression of jGCaMP8s in striatal direct pathway medium spiny neurons (D1 MSNs) ...BGHpA (Alias: CN4035) Expression of CoChR-EGFP in striatal indirect pathway medium spiny neurons (D2 MSNs...BGHpA (Alias: CN4033) Expression of CoChR-EGFP in striatal direct pathway medium spiny neurons (D1 MSNs) ...

...KILLER, KILLER/DR5, TRAIL-R2, TRAILR2, TRICK2, TRICK2A, TRICK2B, TRICKB, ZTNFR9 TNFRSF10C tumor necrosis factor... to a specific trigger. Symbol Name Synonyms ACVR1B activin A receptor, type IB ACTRIB, ACVRLK4, ALK4,... A receptor, type IIA ACTRII, ACVR2 ACVR2B activin A receptor, type IIB ACTRIIB, ActR-IIB, MGC116908 ACVRL1...secretagogue receptor - GIP gastric inhibitory polypeptide - GIPR gastric inhibitory polypeptide receptor...FMRFAL NPPA natriuretic peptide precursor A ANF, ANP, ATFB6, CDD-ANF, PND NPPB natriuretic peptide precursor...

... Maguire and colleagues, other researchers have tried to modify it to improve yields. For example, transfecting...for isolating exo-AAVs rely on differential centrifugation steps to separate cell culture supernatant ...

...started learning proper cell culture technique, I tried to find an app that could help me with calculations...information in the app is restricted to the NEB inventory only, the restriction enzyme sequence resource...This post was contributed by guest blogger Sean D. Stacey. Opinions on the apps discussed in the article...comes in handy when I use protocols that require restriction enzymes or PCR. The app contains a double digest...digest tool that is only helpful for NEB restriction enzymes since the instructions use NEB buffers. Nonetheless...name to discover all the information about that restriction enzyme or share it via email with a colleague...colleague. The app is also great if you’re adding a restriction site to a primer, you only need to skim the alphabet...

...H1 ALS Patrick Aebischer 10881 pCCL cPPT PGK EGFP WPRE LTR H1 shSOD1mismatch SOD1 H1 ALS Patrick Aebischer...CMV Parkinson's Patrick Aebischer 36070 pAAV asyn S87A/S129A SNCA CMV Parkinson's Patrick Aebischer 36071... neuron disease Trina Schroer 51221 CMV-CC2 DCTN1 CMV ALS, motor neuron disease Trina Schroer 51412 CMV-p150...EGFP OPTN GFP CMV ALS Beatrice Yue 68839 pOPTN1-209-EGFP OPTN GFP CMV ALS Beatrice Yue 68840 pOPTN1-424...EGFP OPTN GFP CMV ALS Beatrice Yue 68841 pOPTN217-577-EGFP OPTN GFP CMV ALS Beatrice Yue 68842 pOPTN217-...EGFP OPTN GFP CMV ALS Beatrice Yue 68843 pOPTN425-577-EGFP OPTN GFP CMV ALS Beatrice Yue 68846 pR96L-EGFP...pR96L-EGFP OPTN GFP CMV ALS Beatrice Yue 68847 pD474N-EGFP OPTN GFP CMV ALS Beatrice Yue 68848 pE478G-EGFP OPTN...

...pAAV-hSyn-dF-HA-KORD-IRES-mCitrine kappa-opioid receptor (KOR) - Inhibition IRES mCitrine Cre-dependent 8 Bryan...-DIO-HA-hM3D(Gq)-IRES-mCitrine hM3D(Gq) - Activation HA fusion and IRES mCitrine Cre-dependent 8 Bryan...-DIO-HA-hM4D(Gi)-IRES-mCitrine hM4D(Gi) - Inhibition HA fusion and IRES mCitrine Cre-dependent 8 Bryan...pAAV-CaMKIIa-HA-hM4D(Gi)-IRES-mCitrine hM4D(Gi) - Inhibition HA fusion and IRES mCitrine none 2, 8 Bryan Roth ...pAAV-GFAP-HA-rM3D(Gs)-IRES-mCitrine rM3D(Gs) - Activation HA fusion and IRES mCitrine none 5 Bryan Roth 50478...228902 pAAV-CAG-DIO-HA-HCARD-P2A-mCitrine HCAD(Gi) - Inhibition mCitrine Cre-dependent 8 Bryan Roth No available...element Tag Fusion tags mCherry HA Non-fusion tags mCitrine EGFP dTomato Activity Cre-dependent Flp-dependent...

...-CDS issues. Prohibited Restriction Sites: EcoRI, XbaI, SpeI, PstI Restriction Sites to Avoid: NotI For...Coli genome. Prohibited Restriction Sites: EcoRI, SpeI, NheI, PstI Restriction Sites to Avoid: PvuII, ... use of assembly standards – combinations of restriction sites which preserve a standard prefix and suffix...assembly standards most commonly used by part registries, including the specific prefix and suffix sequences... reverse complimented for primer design. The restriction enzymes used for assembly are the prohibited ... Employs high-efficiency enzymes. Prohibited Restriction Sites: EcoRI, BglII, BamHI, XhoI For more info...giving in-frame scar encoding Thr-Ala. Prohibited Restriction Sites: EcoRI, XbaI, SpeI, PstI For more info,...

...Expression mCitrine 516 529 59 5.7 Monomer (A206K) mCitrine-N1 - Mammalian Expression mCitrine-C1 - Mammalian...Sea urchin pET mCitrine LIC cloning vector (u-mCitrine) - Bacterial Expression mCitrine-pBAD - Bacterial...Expression mAmetrine 406 526 26 6 1.8 min Monomer (A206K) pBad-mAmetrine - Bacterial Expression mAmetrine-N1 ...Mammalian Expression pCS2+8CmCitrine - Zebrafish/Xenopus/Worm/Sea urchin pCS2+8NmCitrine - Zebrafish/Xenopus...mAmetrine-N1 - Mammalian Expression mAmetrine-C1 - Mammalian Expression LSSmOrange 437 572 23 5.7 2.3 h Monomer pLSSmOrange-C1...

...acceptor) FRET Patrick Daugherty 18084 54856 pBad-mAmetrine1.1 tdTomato-pBAD mAmetrine1.1 (donor) tdTomato...Roger Tsien 54553 54723 mTFP1-pBAD mCitrine-pBAD mTFP1 (donor) mCitrine (acceptor) FRET/Dual FRET Robert...ECFP (donor) Citrine (acceptor) FRET Wolf Frommer 65617 pFLIP42 mCerulean (donor) Citrine (acceptor) FRET...being used for their distinct features. Addgene distributes many plasmids that can help researchers plan ...library for the generation of FRET biosensors for ratiometric measurements. The MoBiFc Toolkit (Addgene #1000000222...Sphingomonas sp. , Caulobacter crescentus , Paracoccus denitrificans , Methylobacterium extorquens Julia Vorholt ...

...opens in a new window) ClinicalTrials.gov - a list of Rett syndrome clinical trials (Link opens in a new...in a new window) PMID: 17289941 2010 - Allyson Muotri's lab develops the first iPSC model for Rett syndrome...: 26944080 (Link opens in a new window) Alysson Muotri Q83X C247T Q83X M Fibroblasts & iPSC (Link opens...: 26944080 (Link opens in a new window) Alysson Muotri c.806delG 806delG G269Afs*20 M Fibroblasts & iPSC...new window) HRV Metrics Toolbox - algorithms for data analysis to calculate heart rate variability (Link...in Australia: a review of the epidemiology. J Pediatric . 148(3):347-352. (Link opens in a new window)...Return to top Help us improve this resource by contributing your plasmids! To get started with your deposit...

...tools are abundant! In this post, we will review tried-and-true protein expression control tools as well...

...marker at non-physiological levels. Scientists have tried varied approaches to combat these issues. One method...maintained when grown on media containing the missing nutrient. Scientists can exploit these host mutations by...auxotrophy. When grown on media NOT containing the nutrient, the host cells will die unless they have incorporated...  Marcy Patrick...

...these elusive teachers, supporters and advisors? I tried to start a more formal mentoring program at my company...They all found the support of the group to be intrinsic to their success. Their stories made it clear ...Goal setting is easier with more perspectives contributing to the process Senior people (of which there...

...undergo stringent quality control by Addgene or a trusted quality control partner before distribution. Details...Production Antibody Production Many antibodies distributed by Addgene have been produced in-house by Addgene...contacting [email protected] . Some of the antibodies distributed by Addgene were developed by the Institute for..., antibodies undergo a buffer exchange using centrifugal columns. The final formulation buffer is phosphate-buffered.... Only antibody samples with >90% purity are distributed. Quality Control Each lot of antibody undergoes...

...fingers that recognize various nucleotide triplets. With some trial and error, ZF arrays are able to recognize...zinc finger (ZF) nuclease. Zinc fingers recognize triplets and the FokI nuclease operates as a dimer, cutting...their limitations, however. Not every nucleotide triplet has a corresponding zinc finger, and interactions... targets are confined to sequences composed of triplets with corresponding zinc fingers, potential targetable...genome are every 500 bp. TAL effectors have some restrictions (for example, the target must start with a T...

...light orange, that bind to a specific antigen, triggering an immune response. The stem of the Y structure...single antibody signal. Here, HRP is used as a colorimetric, in which the reaction produces a colored product...the amount of enzyme present in each well. In colorimetric assays, protein amount can be determined by ..., lyse, and then collect protein extract by centrifugation or affinity columns. Mix samples with a primary...immobilized on agarose or magnetic beads. Using centrifugation (for agarose beads) or a magnet (for magnetic... your samples using permeabilization, antigen retrieval, and/or sectioning. Block to reduce non-specific...tissue sections are assayed and the extracellular matrix remains intact. The signaling molecule may be a...

...for efficient recombination? A10: The most we've tried to insert so far has been 1 kb. We used homology...template after performing gDNA purification and have tried conditions with 5% DMSO or without 5% DMSO. My genomic...template, you could then: Confirm your HR by doing Restriction Fragment Length Polymorphism (RFLP) (see Figure...

...Addgene distributes ready-to-use viral preps made from plasmids in our collection! AAV are distributed as ...NeuroMab/Trimmer Lab Recombinant mAb Plasmid Collection : The NeuroMab Facility and James Trimmer have produced...Description PI 65417-AAV8 pAAV-hSyn-dF-HA-KORD-IRES-mCitrine AAV packaging vector for hSyn driven KOR DREADD...recombination Ofer Yizhar Antibodies Addgene distributes ready-to-use recombinant antibodies made from...that are currently in our repository. AAV are distributed as 100 µL of purified viral particles in solution...

...subjected to quality control by Addgene before being distributed to customers. Details about our production protocols...control are described below. AAV Production AAV distributed by Addgene has been produced either in-house ...preparations are purified by iodixanol gradient ultracentrifugation and concentrated to purity and titers adequate...preparations is assayed by comparing the relative stoichiometric ratios of the viral capsid proteins VP1, VP2...containing lentivirus is first cleared by low-speed centrifugation and then by filtration through a 0.45 µm polyethersulfone...

...selection. See plasmid 21916 for neomycin selection. Dmitri Wiederschain 11578 pSico 3rd Conditional (Cre-lox...shRNA. See Szulc et al. (2006) for more variants. Patrick Aebischer and Didier Trono 11795 pLL3.7 3rd Expresses...selection. See plasmid 21915 for puromycin selection. Dmitri Wiederschain 36084 pLV-mCherry 3rd CMV driven expression...expressing envelope plasmid. Arthur Nienhuis and Patrick Salmon 17532 pLTR-G Expression of VSV-G glycoprotein...VSV-G-expressing envelope plasmid. Arthur Nienhuis and Patrick Salmon 22501 pHEF-VSVG VSV-G-expressing envelope...