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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 241 results for: Xpa

Showing: 41 - 60 of 241 results

  1. CRISPR 101: Cytosine and Adenine Base Editors

    Type
    Blog Post
    ...made improvements to base editors, including: expanding targeting scope improving editing efficiency decreasing...new base editors with distinct PAM sequences, expanding the number of available target sites for base ...development of near-PAMless Cas9 variants significantly expands the library of accessible genomic targets for base...variants, BE3 editing window variants   Greatly expands the number of target loci for base editing Rees...2019). Continuous evolution of base editors with expanded target compatibility and improved activity. Nature...

  2. Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone

    Type
    Blog Post
    ...they meet other Mentors and Singletons, and vastly expand their networks as they naturally find people with...briannadoesbio) June 22, 2019 Favorable conditions - Expansion of networks The last stage is Exponential Growth...mentoring in STEM, including Addgene’s Joanne Kamens. Expanding Binning Singletons to other scientific conferences...such mentoring programs, scientists can build and expand their networks and further their career development...

  3. Validated gRNA Sequences

    Type
    Collection
    ...gRNA sequence spreadsheet". Thanks for helping us expand and improve our resources! Download validated gRNA...

  4. AAV Packaged on Request is Here!

    Type
    Blog Post
    ...AAV Packaged on Request, an expansion of our viral vectors service, has officially launched! As of today... excited for all the ways this much-requested expansion will allow us to better serve our community. If...If you’re curious about the service, read on.  Expanding our viral vector service Addgene has offered in-stock...

  5. Providing Plasmids to Researchers in Developing Countries: Addgene and Seeding Labs Team Up

    Type
    Blog Post
    ...Seeding Labs, a nonprofit founded in 2007, strives to expand the opportunities and scientific resources available...first step; going forward, Seeding Labs hopes to expand its activities by facilitating connections to mentorship...regions like South America and Africa, but we need to expand our efforts to find new and creative ways to reach...

  6. Hot Plasmids and Viral Preps - July 2021

    Type
    Blog Post
    ...physiology, development, and disease. In order to expand their model’s impact, organoid users have recently...months: Our selection of GCaMP8 AAV preps has expanded. Check out the new selection of ready-to-use AAV9... INTRSECT 2.0 calcium sensor available in AAV8! Expand your imaging experiments with the Cre-OFF/FLP-ON...

  7. New and Upcoming Viral Vectors - December 2019

    Type
    Blog Post
    ...for light mediated neuronal control and we are expanding our viral service to make these readily available... experiment. The Addgene viral service has now expanded to offer many of our control plasmids in new serotypes... is vital in studying neural networks. We have expanded our calcium sensor collection to include our first...

  8. 15 Hot Plasmids from 2017

    Type
    Blog Post
    ...).This toolbox adds new neuronal tools to the expanding gateway compatible vectors currently shared amongst...Podcast Segment    Photocleavable protein PhoCl expands optogenetics toolbox Increasingly popular Optogenetics...proteins (FP) for cellular imaging is constantly expanding. Case in point - check out the growing list of...new base editors with distinct PAM sequences, expanding the number of available target sites for base ...

  9. Addgene’s Viral Service - Why Virus? Why Now?

    Type
    Blog Post
    ...hundreds of scientists about various ways we might expand Addgene’s services. There was an overwhelmingly... to facilitate the formation of partnerships to expand the service and figure out what we can afford to...educational content via the Addgene Blog and our expanding website resources. I hope this gave you the impression...

  10. Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity

    Type
    Blog Post
    ...Our previous blog post The PAM Requirement and Expanding CRISPR Beyond SpCas9 described the PAM specificity...toolkit available to researchers will continue to expand. Final thoughts Since the first use of Cas9 to ..., et al. "Engineered CRISPR-Cas9 nuclease with expanded targeting space." Science 361.6408 (2018): 1259...

  11. Addgene: The First Twenty Years

    Type
    Blog Post
    ...celebrating Addgene's 10 year anniversary.   Expanding our work In March 2015, Addgene reached the 500,000...over 114,600 viral preps. And in 2025, we’ll be expanding our viral vectors service to include AAV Packaged...slowed, Addgene began to think about how we could expand our support for the research community once more...

  12. CRISPR 101: Cas9 vs. The Other Cas(s)

    Type
    Blog Post
    ...enzyme. Fast facts – PAM requirement: NGG and an expanding number of others. Best for: a wide range of DNA...scientist toolbox. If your genome engineering goals expand beyond generating frameshift mutations or single...my CRISPR experiment? The PAM requirement and expanding CRISPR beyond spCas9 Cas proteins More resources...

  13. Transferable Skills Guide: External Collaborations

    Type
    Blog Post
    ...below). Expand: Collaboration opportunities outside an organization The next step is to expand your efforts... to start close to your comfort zone and slowly expand from there. Start small: Collaboration opportunities...

  14. CRISPR-mediated Plant Base Editors

    Type
    Blog Post
    ...plant base editors. Plant CBE and ABE with expanded PAM compatibility All of the base editors mentioned.... Hua, Kai, Xiaoping Tao, and Jian‐Kang Zhu. "Expanding the base editing scope in rice by using Cas9 variants.... PubMed PMID: 29867128. 11. Li, Chao, et al. "Expanded base editing in rice and wheat using a Cas9-adenosine...

  15. Using Phosphoserine to Study Protein Phosphorylation

    Type
    Blog Post
    ...modified through phosphorylation (1). However, the expansion of studies on protein phosphorylation in an array...PubMed PMID: 15790858. 5. Park, Hee-Sung, et al. "Expanding the genetic code of Escherichia coli with phosphoserine... Marc J., et al. "Genomically recoded organisms expand biological functions." Science 342.6156 (2013):...

  16. Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands

    Type
    Blog Post
    ... of more stable, brighter, and specific dyes, expanding the capabilities of fluorescence-based techniques...® system, the new Janelia Fluor HaloTag Ligands expand the self-labeling capabilities of this platform...Rampioni, G., Visca, P., & Kolmakov, K. (2023). Expanding the microbiologist toolbox via new far-red-emitting...

  18. 2022 and 2023: Reflections and Looking Forward

    Type
    Blog Post
    ...information sharing, and research reproducibility while expanding our suite of materials and services to support...many exciting things planned in 2023, from the expansion of our Data Hub to process improvements in every...

  20. Embracing Serendipity: A Crucial Element in the PhD Journey

    Type
    Blog Post
    ...inviting serendipity into your research lies in expanding the scope of your exploration. While it is essential...diverse literature. By delving into new topics and expanding your knowledge base extensively, you open doors...
Showing: 41 - 60 of 241 results