Hot Plasmids - June 2020 - Barcoded CRISPR Library, Sparse Cell Labeling, Calcineurin Reporter, and DNA Staining Dye Alternative
Type
Blog Post
...manipulations such as injection of precise titrations of AAV or heat-shocking the organism.
The SPARC system uses.... https://doi.org/10.1101/788679
A destabilized GFP reporter for calcineurin activity in yeast
Article...reporter consisting of a quickly-degraded destabilized GFP variant fused to a 4X CDRE repeat. In addition to... Ca2+ (bottom panels) equipped with destabilized GFP calcineurin reporter (blue) and stained with PI (...death. Image from Diessl et al., 2020.
Find the GFP reporter calcineurin activity here
Diessl et al.,...fluorescent protein to the DNA. They created plasmids for purification of tTALE-eGFP and tTALE-mCherry.
In comparison...
Figure 1: Initial steps in the CRISPR-pooled screening using sgRNAiBAR. Image from Zhu et al., 2019.
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