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We narrowed to 74 results for: acr Anti-CRISPR

Showing: 41 - 60 of 74 results

  1. Plan Your Experiment

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    Collection
    ... Science Guides CRISPR Guide Plan Your Experiment Plan Your CRISPR Experiment Jump To: Plan ...information on the wide range of CRISPR technologies, visit our CRISPR Guide . There are many different...framework to get you started using CRISPR in your research and setting up CRISPR experiments. We will focus on...systems utilized for CRISPR/Cas gene editing. This image summarizes ways that the CRISPR/Cas system can be...transcription elongation. CRISPR Activation : Target the transcription start site. CRISPRa requires dCas9 to ...Needs a good antibody References Cong, L., & Zhang, F. (2015). Genome engineering using CRISPR-CAS9 system...Learn how to plan a basic CRISPR experiment, including selecting the edit type, designing and cloning...

  2. CRISPR Guide

    Type
    Guide
    ...naturally-occurring Acr (Anti-CRISPR) proteins can help control CRISPR activity. Acr proteins have evolved...Guide CRISPR Guide Jump To: CRISPR Guide Overview CRISPR Basics Small Edits Large Edits Genome-Wide Screening...target tissue. Browse Plasmids: Anti-CRISPR Small Precision Edits CRISPR can be used to make small, very...Figure 7: Overview of CRISPR transposases. Browse Plasmids: CRISPR transposases CRISPR Recombinases and Integrases... using CRISPR libraries. What Are Pooled Lentiviral CRISPR Libraries? Pooled lentiviral CRISPR libraries...References Website CRISPR plasmids and resources at Addgene CRISPR 101 eBook (3rd edition) CRISPR software and... Addgene Blog CRISPR topic page How to design your gRNA for CRISPR genome editing CRISPR 101: Homology...

  3. Four Base Editing Reporters to Monitor and Enrich Editing in Real-time

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    Blog Post
    ...other CRISPR reporters Browse our CRISPR blog posts Resources on Addgene.org Browse the CRISPR guide... Conversion: A Versatile Assay for the Quantification of CRISPR/Cas9-mediated Genome Editing. Molecular...the genome, but they’re inefficient compared to CRISPR/Cas9 edits that rely on double strand DNA breaks...converted into BFP (blue fluorescent protein) upon CRISPR/Cas9 homology directed repair (HDR) (Glasser et...guide Find CRISPR plasmids for your research ...editing by APOBEC-Cas9 Though the ACE reporter quantifies APOBEC-Cas9 efficiency in real-time, it relies...Harris Lab created a panel of eGFP reporters to quantify the on-target DNA editing efficiency of APOBEC-Cas9...

  4. 22 Hot Plasmid Technologies from 2014

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    Blog Post
    ...For more information on these new CRISPR screening tools, see our CRISPR/Cas Plasmids: Pooled Libraries ...Lentiviral CRISPR Libraries Enable Genome-Scale, Knockout Screening. Also, check out our updated CRISPR-Cas ...CRISPR-Cas resources at www.addgene.org/CRISPR/ to browse CRISPR plasmids, watch informational videos, download...for background information on CRISPR technology? See our improved CRISPR-Cas Guide. Wang et al., Science...Tet-inducible CRISPR activators and repressor plasmids. (Kearns et al., Development. 2014..) CRISPRs for Xenopus.... 2014..) Our CRISPR-Cas collection of plasmids updates frequently, so visit our CRISPR pages often to...Microbiology 2011 Feb 22;11:38. Lentiviral CRISPR libraries for knockout screening New systems have...

  5. Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME

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    Blog Post
    ...using the CRISPR-Cas9 system CHIME Article contributed by Aliyah Weinstein  Listen to the CRISPR podcast...require custom nucleases for each gene of interest. CRISPR-Cas9 tools have been used in immunology for in ...vitro screens of immune cell activity. However, a CRISPR-based tool for in vivo functional analysis of immune...Harvard Medical School developed a solution: CHIME, a CRISPR-Cas9 system that can edit genes in any immune lineage...regulators of post-transcriptional gene expression across the majority of cellular pathways. Despite several...efficient gene deletion in B cells, T cells, macrophages, and dendritic cells, with no deleterious effects...immune response other than what would be the anticipated effect of the targeted gene. CHIME can also be...

  6. CRISPR Plasmids - Empty gRNA Vectors

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    ...Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts CRISPR requires... CRISPR Empty gRNA Vectors CRISPR Plasmids: Empty gRNA Expression Vectors Browse CRISPR Plasmids...plasmids and therefore are not limited to a single CRISPR function. Alternatively, gRNA-only plasmids can...gRNA sequence, such as BsbI or BsaI Cas9 (or other CRISPR) system that the vector was designed to be used...to be used with. crRNAs used with Cpf1 or other CRISPR systems are also listed here and denoted as such...enzymes? CasPEDIA is an encyclopedia of Class 2 CRISPR systems with wiki entries describing enzyme activity...Plasmids Expression System Description PI Multiplex CRISPR/Cas9 Assembly System Kit Mammalian A system for...

  7. Viral Vectors 101: Preparing Pooled Libraries

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    Blog Post
    ...screening, and validation of CRISPR libraries. The Genome Engineering using CRISPR/Cas Systems Forum- Moderated...Lentiviral CRISPR Libraries- A nice overview of what to consider when using pooled lentiviral CRISPR libraries...but we do distribute several of our most popular CRISPR pooled libraries and one of our most popular barcoding...Resources and references References Genome-scale CRISPR-Cas9 knockout and transcriptional activation screening...Practical Considerations for Using Pooled Lentiviral CRISPR Libraries. Curr Protoc Mol Biol. 2016 Jul 1;115..., Brigham MD, Sanjana NE, Zhang F. Genome-scale CRISPR-Cas9 knockout and transcriptional activation screening...conditions to optimize include incubation time, antibiotic conditions, and amount of polybrene. (psst…didn...

  8. Plasmids 101: Modular Cloning Applications and Kits

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    Blog Post
    ...more of these kits, as there are many options! CRISPR CRISPR-Cas can harness the power of MoClo to expand...repurposed for CRISPR applications (gene insert = Cas9 + gRNA), Addgene distributes specialized CRISPR kits too...including conditional gene expression, multiplexed CRISPR, and chromosomal integration of larger expression...targetability and effectiveness. Perhaps the most intuitive CRISPR MoClo application is to assemble multiple unique...edit optimization, and more. While there are many CRISPR-Cas libraries and tools available for certain model...biologists, Addgene has not one, not two, but three CRISPR MoClo kits for plants that work for both monocots...MoClo can help you achieve it! More recently, a CRISPR MoClo kit termed Fragmid was developed for mammalian...

  9. Addgene: The First Twenty Years

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    Blog Post
    ...And when CRISPR arrived in 2012, our repository was rapidly able to support sharing of CRISPR constructs...information across the globe.  “Read the Addgene guide: https://www.addgene.org/guides/crispr/” — SirHamhands...responding to a request for “any suggestion for CRISPR online course for beginners” on r/labrats, Reddit...library of antibody educational resources, including our new Antibodies 101 eBook, the Antibody Guide, and...ready-to-use recombinant antibodies service, part of the Neuroscience AntiBody Open Resource (NABOR) platform...comprised of 1302 plasmids, was shared with researchers across the globe. As the pandemic slowed, Addgene began...-use viral vectors were useful for researchers wanting high quality reagents, and we realized we could...

  10. Exploring Applications of the Bioluminescent HiBiT Tag

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    Blog Post
    ...function of tagged proteins and facilitates precise CRISPR-based tagging, or low-levels of ectopic expression...range of workflows, from classic overexpression to CRISPR knock-in, letting you track endogenous protein ...detail on whether the authors incorporated HiBiT by CRISPR tagging at the endogenous locus or used other expression...kinase essential for cell proliferation signaling. CRISPR knock-in HiBiT-tagged EGFR cells allowed researchers...Illuminating Transmembrane Proteins Using HiBiT CRISPR Cell Lines. Promega Notes. https://www.promega....illuminating-transmembrane-proteins-using-hibit-crispr-cell-lines/ Shibata, C., Otsuka, M., Shimizu, T...native protein context, but they still depend on antibody-based detection or western blotting, methods that...

  11. Hot Plasmids: Summer 2024

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    Blog Post
    ...s Neuroscience Antibody Collection to find a recombinant anti-GFAP [N206A/8R] antibody shared by James...well as antibodies for identifying other cell types in the nervous system!  Find recombinant anti-GFAP [...N206A/8R] antibody here!   Figure 5: Free-floating rat brain sections stained with Anti-GFAP. The...Genome-wide programmable transcriptional memory by CRISPR-based epigenome editing. Cell, 184(9), 2503–2519...months we highlight some of the new plasmids, antibodies, and viral preps in our repository through our...CHARM: A compact epigenetic silencer Recombinant antibodies for marking astrocytes MagIC-Cryo-EM for sample... determination on magnetic beads for scarce macromolecules in heterogeneous samples. bioRxiv 2024.01.21.576499...

  12. PRIDICT: Predicting Efficiencies of Prime Editing Guide RNAs

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    Blog Post
    ...Your gRNA for CRISPR Genome Editing PRIME Editing: Adding Precision and Flexibility to CRISPR Editing Resources...Resources on Addgene.org CRISPR: PRIME Edit Collection The CRISPR Guide Empty gRNA Expression Vectors...highlighted how chromatin substantially affects prime editing outcomes across many genomic locations. In...2023), their predictive power is sometimes limited across different targets due to variations in chromatin...learning prediction of prime editing efficiency across diverse chromatin contexts. Nature Biotechnology...

  13. Five Popular Model Organisms, Part 2

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    Blog Post
    ...101 blog series Learn more about CRISPR and genome editing in our CRISPR 101 blog series Resources on Addgene.org... in gene modification tools and methods such as CRISPR have vastly improved scientists’ ability to use...use in the axolotl including plasmids for use in CRISPR and Tol2 transposase induced transgenesis.  Xenopus...Xie, Z., Zhang, C., & Xing, X.-H. (2018). Pooled CRISPR interference screening enables genome-scale functional...regulate regeneration and wound healing are conserved across species, studying the axolotl may reveal the potential...10.1111/j.1365-313x.2009.04086.x Krämer, U. (2015). Planting molecular functions in an ecological context with...

  14. AAV Q&A with Tim Miles

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    Blog Post
    .... The continued development of more and smaller CRISPR tools is an exciting area for future AAV application...- a primer for beginners Adenoviral delivery of CRISPR/Cas9 Tips for a first time AAV user Resources ...and somewhat more reliable quantitation. Differences in sample treatment across methods can affect reported...advise that users keep titering method consistent across samples within a project. Targeting Your AAV Q4...e.g. first at 2 months, then at 4 months? Or do antibodies become a prohibitive problem? A: Re-administration...for AAV due to the development of neutralizing antibodies in animal models. All of our engineered AAV are...

  15. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

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    Blog Post
    ...purposes, like creating stable cell lines, delivering CRISPR/Cas9 to cells for genome editing, and introducing... is gradually reduced. For example, after using CRISPR/Cas9 to edit a gene, the expression of Cas9 is ...High-frequency off-target mutagenesis induced by CRISPR-Cas nucleases in human cells. Nature Biotechnology...molecular biology for over three decades. Widely used across various research applications, they have become...lentiviral vectors, the lentiviral genome is distributed across multiple plasmids. For simplicity, we’ll use the...isolating genomic DNA from transduced cells and quantifying the integrated viral DNA along with a reference...detecting vector particle components or performing quantitative PCR titration of vector DNA in samples harvested...

  16. Twenty Years of Sharing: Expanding Accessibility

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    Blog Post
    ...Educational Resource Top Five by Views Guides CRISPR Guide Lentiviral Guide AAV Guide Optogenetics Guide... What is a Plasmid? How to Design Your gRNA for CRISPR Editing The Promoter Region - Let's Go! How to ...primers to help users get started with tools like CRISPR, lentivirus, and optogenetics — and shared the ...to identify and reduce barriers for scientists wanting to share their tools with Addgene.  In our next...materials both available and accessible to scientists across the globe?” By asking this question, and by listening...Addgene blog Twenty Years of Addgene Sharing: CRISPRTwenty Years of Sharing: Addgene's Viral Vector Service...

  17. Hot Plasmids: Summer 2025

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    Blog Post
    ... TnpB, so hot right now By Alyssa Shepard While CRISPR has become a household name, the search for smaller...more in our recent blog post on TnpB). Similar to CRISPR-Cas, TnpB is directed to genomic targets using ...months we highlight some of the new plasmids, antibodies, viral preps, and more in the repository through...fused to mVenus, mGold2s, or mGold2t. Bottom: Quantification of photostability. Image reused from Lee & ...ATLAS (anterograde transsynaptic label based on antibody-like sensors) changes that. Developed by the Arnold... Arnold Lab, ATLAS is based on an antibody-like protein AMPA.FingR (AF) that binds to AMPA receptor subunit...regions in mice, ATLAS mediated anterograde labeling across single synapses, a key attribute for neuronal tracing...

  18. Neurodegeneration Plasmid Collection

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    Collection
    ...Babu 128616 Anti-Kv1.1 K+ channel [K20/78R] KCNA1 CMV Episodic ataxia James Trimmer 128627 Anti-Kv4.3 K+ ...Harper 177511 Anti-Frataxin [N191/7R] FXN CMV Friedreich ataxia James Trimmer 177520 Anti-LRRK2/Dardarin...177537 Anti-Kv3.3 K+ channel [N375/67R] KCNC3 CMV Spinocerebellar ataxia James Trimmer 177546 Anti-PINK1...182104 Anti-LRRK2/Dardarin, N-terminus [N138/6R] LRRK2 CMV Parkinson's James Trimmer 182113 Anti-Kv3.3 ...188155 Anti-Kv4.3 K+ channel [K75/18R] KCND3 CMV Spinocerebellar ataxia 19 James Trimmer 188163 Anti-VAPA...188167 Anti-IP3 receptor, type 1 [L24/21R] ITPR1 CMV Spinocerebellar ataxia James Trimmer 188171 Anti-IP3...188177 Anti-IP3 receptor, type 1 [L24/1R] ITPR1 CMV Spinocerebellar ataxia James Trimmer 188185 Anti-IP3 ...

  19. SunTag and Fluorescent Imaging

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    Blog Post
    ...applicable across many areas of research. Beyond fluorescence, SunTag can also be used to improve CRISPR-based... how its localization and expression may change across various conditions. However, this system is far...GCN4. GCN4 recruits GFP fused to the cognate scFV antibody, which is expressed from a separate plasmid. This... track run lengths of the motor protein kinesin across microtubules. Tanenbaum et al. then tested their...

  20. Cancer and the Immune System: Deciphering the Relationship

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    Blog Post
    ... first ever CRISPR-based clinical trial in 18 lung cancer patients. This study used CRISPR gene editing...are a few of the reasons antibodies may result in a lack of response. Antibodies targeting the tumor-augmenting...effectively in mice. Chimeric antigen receptors T cells producing chimeric antigen receptors (CARs) on their...acquire genetic alterations and alter cell-surface antigen production to evade the immune system. 3. Escape...sidestep immune surveillance: Suppressing Function Antigen presenting cells (APCs) that would normally alert...regulates the expression of the tolerance-producing antigens enunciated above. Finally, signals released from... produces modified dendritic cells (DCs) and macrophages (M1 and M2) that help create welcoming environments...
Showing: 41 - 60 of 74 results